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PELATIHAN AKLIMATISASI BIBIT STROBERI HASIL KULTUR MERISTEM BAGI PETANI STROBERI PANCASARI R. Dwiyani; K.B. Susrusa; I.A.P. Darmawati; G.N.A.S. Wirya; I.G.A. Gunadi; N.N.A. Mayadewi; H. Yuswanti; K.A. Yuliadhi; T.A. Phabiola; R. Kawuri; Y. Fitriani
Buletin Udayana Mengabdi Vol 22 No 3 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i03.p10

Abstract

Abstracts The community service activity entitled "Training for Acclimatization of Strawberry Seeds from Meristem Culture for Pancasari Strawberry Farmers" has been completed. This activity was motivated by the problem of seeds by strawberry farmers in Pancasari, where farmers needed healthy seeds from tissue culture, because through propagation with runners, crop yields decreased drastically due to virus attacks. The solution is the use of seeds from meristem culture. Acclimatization training by farmers is important because removing seeds from culture bottles and then growing them on media ex vitro requires skills and understanding of acclimatization theory. The purpose of this service was to provide strawberry acclimatization skills to farmers, so that farmers can acclimatize themselves to meristem cultured strawberries produced by the Tissue Culture Laboratory of the Faculty of Agriculture Udayana University. This activity was based on the results of strawberry meristem culture research in 2019 funded by the ministry of research technology and higher education Republic of Indonesia through a national competitive grant. The results of the current community service concluded several things as follows: The training participants, especially farmers, have succeeded in carrying out their own acclimatization practice; Strawberry seedlings grown in the highlands (Pancasari) had better vigor than those grown in the lowlands (Denpasar). Keywords: acclimatization, meristem culture, Pancasari, strawberry, virus.
The Role of Mycorrhizae on the Growth of Banana Planlets of Cultivar Raja (Musa Paradisiaca C.V. Raja) from Bali at Post Acclimatization: Role of Mycorrhizae on the Growth of Banana Planlets Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta; Gunadi, I Gusti Alit; Darmawati, Ida Ayu Putri; Yuswanti, Hestin; Susrusa, Ketut Budi; AStiningsih, Ana Agung Made
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.11

Abstract

The effect of inoculation of a combination of the arbuscular mycorrhizal fungus of Glomus and Acaulospora on micropropagated banana plantlets cultivar Raja from Bali during the acclimatization phase was investigated. The experiment was conducted at the Experimental Station of the Faculty of Agriculture, Udayana University, Denpasar, Bali, Indonesia, over the period of March to June 2022. The objective of this research was to evaluate the impact of various doses of mycorrhizae on the growth of tissue culture banana plantlets that had undergone acclimatization for a period of six weeks. A total of five different doses of mycorrhiza were applied to the acclimatized plantlets, namely M0=without mycorrhiza and M5=5g mycorrhiza.kg-1, M10=10 g mycorrhiza.kg-1, M15=15 g mycorrhiza.kg-1, M20=20 g mycorrhiza.kg-1. A significant effect was discovered at M15; however, compared to M20, M20 gave better results for root length and fresh weight of the above-ground part, indicating the best dose was 20 g mycorrhiza kg-1. This study concluded that mycorrhiza has a very significant role in supporting the growth of micro propagated banana plantlets at post-acclimatization. Variables observed, such as the average number of leaves, stem diameter, above-ground plant fresh and dry weight, and root fresh and dry weight, increased with the application of AMF (Glomus and Acaulospora).
A new report of moss-camouflaging mantis Nanomantinae in Bali, Indonesia Sudiarta, I Putu; Wirya, Gusti Ngurah Alit Susanta; Selangga, Dewa Gede Wiryangga; Sanjaya, I Gusti Ngurah Prabu Wira; Arimbawa, I Made; Damastra, Garda Bagus
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.2231-6

Abstract

In 2019 we found moss praying mantis around the forest in the Bedugul Area of Bali, Indonesia. This species belongs to the family Mantidae, subfamily Nanomantinae, and predicted as member of  Calofulcinia genus based on diagnostics of morphological characteristics, behavior, and habitat. Information about the morphological characteristics and data from the molecular sequencing database of the genus Calofulcinia has been very limited. In this research, the barcoding DNA of mitochondrial Cytochrome Oxidase I (mtCOI) of the species is noted using paired primers LCO1490 and HCO2198. Based on the sequence analysis (identity matrix and phylogeny analysis), the moss mantis from Bali has low similarity and genetic relationship with other Mantodea species from GenBank database. The data indicate the moss mantis from Bali has not been reported previously. In addition, the distribution of moss mantis species in Bedugul, Bali, Indonesia has just been described and recorded.
First Report of Cladosporium dominicanum Zalar, de Hoog & Gunde-Cim. Infecting Whitefly on Ornamental Plants in Bali, Indonesia Sudiarta, I Putu; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Gargita, I Wayan Diksa; Yuliadhi, Ketut Ayu; Klett, Katrina
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86865

Abstract

As natural enemies, entomopathogenic fungi are essential for controlling certain plant pests, such as whitefly. Many types of entomopathogenic fungi can infect whiteflies, including those from the genera Aschersonia, Metarhizium, Beauveria, and Cladosporium. Currently, there is great interest in using entomopathogenic fungi as an environmentally friendly pest control in organic and sustainable agricultural systems. This study aimed to identify entomopathogenic fungus associated with whiteflies. Identification of entomopathogenic fungus was performed morphologically and molecularly using DNA barcoding with ITS-1 and ITS-4 as primers, in addition to sequencing and phylogenetic tree analysis. Whiteflies infected with entomopathogenic fungus were discovered on ornamental plants (Premna serratifolia, Ficus religiosa, and Ficus rumphii) in Denpasar, Bali, Indonesia from January to March 2023. We found that samples from the field had mycelium that was unbranched or sparingly branched with solitary conidiophores arising terminally from ascending hyphae or laterally from plagiotropous hyphae. Samples from PDA media had ramoconidia with darkened septa, straight and conidia in long branched chains, branching in all directions, conidia were obovoid, ovoid to limoniform. The fungal colonies had the characteristics of being grey-olivaceous, olivaceous-black, and the margins were grey-livaceous to white, gray olivaceous in colony center due to abundant sporulation. In addition, based on the BLAST nucleotide sequence from NCBI GenBank, the insect pathogen phylogenetic tree found in Denpasar, Bali, is in the same clade as Cladosporium dominicanum strain SCAU014, accession number KY827344.1. Based on morphological and molecular analysis the fungus that infects whiteflies on ornamental plants is C. dominicanum.
Occurrence of Cassava Lace Bug Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) in Bali, Indonesia Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Devi, Komang Saraswati; Ariyanta, I Putu Bawa; Wirya, Gusti Ngurah Alit Susanta; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Gargita, I Wayan Diksa; Wiguna, Putu Perdana Kusuma; Yuliadhi, Ketut Ayu; Devi, Putu Shinta
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87438

Abstract

Cassava Lace Bugs (CLB) are native pest of cassava (Manihot esculenta Crantz, Euphorbiaceae) to the Neotropical Region, mainly in Brazil. On the other hand, East Java was the first region in Indonesia to record the presence of CLB in 2021, however, it has not been reported in other regions in Indonesia. Therefore, the very importance to recognise the occurrence of CLB in other regions in Indonesia. Based on this, the research has been carried out starting with a field survey, observing behaviour of insect in the field and identify morphologically in the laboratory. The survey results show that the infestation of CLB has been found in lowland, medium, and highland areas in Bali. Symptoms of infestation on the upper leaf surface are small yellow spots with brownish variations. Based on the identification key, CLB from Bali Indonesia, shows a characteristic of a head with a pair of frontal spines. Based on this evidence, the CLB insect can be identified as Vatiga illudens (Hemiptera: Heteroptera: Tingidae). It is the first report of novel distribution areas for V. illudens in Bali, Indonesia. The results of this research are important because V. illudens is one of the main pests of cassava. 
The First Report of the Occurrence of the Root Mealybug Ripersiella multiporifera Jansen (2008) (Hemiptera: Coccoidea: Rhizoecidae) in Indonesia Devi, Komang Saraswati; Sudiarta, I Putu; Sunari, Anak Agung Ayu Agung Sri; Wirya, Gusti Ngurah Alit Susanta; Devi, Putu Shinta; Eka Wijayanti, Febri
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.89662

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Ripersiella multiporifera is a root mealybug species within the family Rhizoecidae that has distinctive bitubular pores on the dorsal and ventral. This species was first discovered by Jansen (2008) in the Netherlands during an import interception of Sansevieria sp. from Indonesia and Hoya kerrii from Thailand. This species was also found during inspections in Sicily (Italy) on the roots of Sansevieria trifasciata. In Indonesia, there are no reports of the existence of R. multiporifera, emphasising the need for research on its presence. This work was conducted the morphological method based on modified determination key and the molecular method based on MtCOI gene. The identified species was R. multiporifera, and this finding represents the first evidence of R. multiporifera's presence in Bali (Indonesia) which can be used as a reference for future research, especially in population control approaches. 
Jeruk Keprok Tejakula: Potensi Pengembangan Berdasarkan Analisis Kesesuaian Lahan di Kecamatan Tejakula, Kabupaten Buleleng Trigunasih, Ni Made; Rai, I Nyoman; Wijaya, I Nyoman; Wijana, Gede; Wirya, Gusti Ngurah Alit Susanta; Widhianthini, Widhianthini; Yudha, I Kadek Wisma; Utama, I Wayan Eka Karya; Wiraatmaja, Wayan
Bali Membangun Bali: Jurnal Bappeda Litbang Vol 5 No 1 (2024): April 2024
Publisher : Badan Riset dan Inovasi Daerah Provinsi Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51172/jbmb.v5i1.328

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Tujuan: Penelitian yang dilakukan bekerja sama dengan Badan Riset Inovasi Daerah (Brida) Provinsi Bali ini bertujuan untuk mengetahui potensi pengembangan jeruk keprok Tejakula dan sebarannya berdasarkan analisis kesesuaian lahan aktual dan potensial serta arahan pengelolaan lahan, dalam rangka pengembangan jeruk keprok Tejakula. Metode penelitian: Penelitian dilaksanakan di Kecamatan Tejakula Kabupaten Buleleng mulai Mei 2023 sampai November 2023. Metode yang digunakan dalam penelitian ini adalah metode survei dan wawancara. Metode klasifikasi kesesuain lahan ditetapkan dengan cara mencocokkan (matching) data karakteristik/kualitas lahan dengan kriteria syarat tumbuh berdasarkan pedoman Ritung et al. Penilaian kesesuaian lahan dilakukan sampai pada tingkat unit. Hasil dan pembahasan: Hasil penelitian menunjukkan bahwa kelas kesesuaian lahan aktual untuk tanaman jeruk keprok Tejakula tergolong S3 (sesuai marginal) sampai N (tidak sesuai) dengan factor pembatas curah hujan, tekstur, bahan kasar, KTK, N-total, P-tersedia, lereng dan bahaya erosi. Faktor pembatas setelah diperbaiki maka didapatkan kesesuaian lahan potensial tergolong S1 (sangat sesuai) sampai S2 (cukup sesuai) dengan faktor pembatas tekstur tanah. Implikasi: Perbaikan kesesuaian lahan potensial dapat dilakukan dengan upaya perbaikan struktur tanah dengan penambahan pupuk organik. Arahan penggunaan lahan pada lokasi penelitian dengan mengatur waktu tanam, penambahan pupuk organik, pupuk Urea, Pupuk TSP/SP36 dan menerapkan teknik konservasi yaitu dengan terasering dan tanaman penguat teras.
Symptoms variation and molecular characterization of Strawberry vein banding virus in Bali, Indonesia Phabiola, Trisna Agung; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12544-53

Abstract

Vein banding disease has affected young strawberry leaves in Bali over the past five years. Caused by Strawberry vein banding virus (SVBV), the disease is primarily associated with fruit size reduction, though its exact impact remains unclear. This study aims to assess symptom variation, disease severity, and the molecular characteristics of SVBV in Bali strawberry plants. Field observations and molecular identification were conducted using PCR with SVBV-specific primers targeting the CP gene. Samples were collected from ten locations in Bali, including Pancasari, Candikuning, Wanagiri, Gobleg, and Kembang Merta. SVBV DNA from Candikuning, Pancasari, and Kembang Merta was successfully amplified, confirming SVBV infection as the cause of vein banding symptoms. SVBV induces vein banding with upward and downward leaf curling. Disease incidence was highest in Pancasari 1 and 3 (80%) and lowest in Wanagiri 1 and Gobleg (20–28%). Disease severity ranged from 13% to 83%, with the highest recorded in Pancasari, Buleleng, and the lowest in Kembang Merta, Tabanan. Molecular analysis revealed that the SVBV isolate from Bali shares 99.3–100% sequence homology with Chilean isolates. Phylogenetic analysis showed clustering with SVBV isolates from the United States, Brazil, and Chile. This study provides the first molecular characterization of SVBV in Bali, contributing to a better understanding of its epidemiology and potential impact on strawberry production.
The Effect of media types on the growth of insect pathogenic fungi (Aschersonia placenta) Sudiarta, I Putu; Hartha , I Komang Gede Suweca; Yuliadhi , Ketut Ayu; Wirya, Gusti Ngurah Alit Susanta; Selangga, Dewa Gede Wiryangga; Suputra , I Putu Wirya; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12554-62

Abstract

Bali has favorable conditions for the production and expansion of citrus plantations. However, citrus cultivation in Bali often faces challenges, including reduced yield caused by pest infestations and pathogenic diseases. One significant pest affecting citrus plants is the whitefly. A potential method for controlling whiteflies is the use of natural enemies. The entomopathogenic fungus Aschersonia placenta is one of natural enemy that can effectively manage whitefly infestations. However, the succesful utilization of A. placenta requires a specialized approach, particularly in selecting an appropriate growth medium. This study aimed to investigate the impact of different growth media on the development of the entomopathogenic fungus A. placenta. The research began with field sampling, followed by the isolation and morphological identification of A. placenta to obtain a pure culture. Subsequently, media tests were conducted to evaluate fungal growth. Nine treatments were implemented to examine the impact of different media: (1) Water Agar (WA), (2) Potato Dextrose Agar (PDA), (3) Potato Sucrose Agar (PSA), (4) Water Agar + Weaver Ant Eggs Flour (WA-WAEF), (5) Potato Dextrose Agar + Weaver Ant Eggs Flour (PDA -WAEF), (6) Potato Sucrose Agar + Weaver Ant Eggs Flour (PSA-WAEF), (7) Water Agar + Cricket Flour (WA-CF), (8) Potato Dextrose Agar + Cricket Flour (PDA-CF), and (9) Potato Sucrose Agar + Cricket Flour (PSA-CF). The results demonstrated that incorporating cricket insect flour (Gryllidae: Orthoptera) or weaver ant egg flour (Oecophylla smaragdina) into potato sucrose media provided the most optimal growth environment for A. placenta.
Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
Co-Authors Abd. Rasyid Syamsuri Agung, I Dewa Agung Putra Anak Agung Istri Kesumadewi ARIYANTA, I PUTU BAWA AStiningsih, Ana Agung Made Chiharu Hongo Damastra, Garda Bagus DEBBIE OKTAVIANI DEPARI Devi, Komang Saraswati Devi, Ni Luh Putu Hartika Sinta Devi, Putu Shinta Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Ngurah Suprapta Dharmadiatmika, I Made Agus Dinarkaya, Shah Mahapati DWI WIDANINGSIH Eka Wijayanti, Febri Emilia Simpllisiu Ake Wangge GARGITA, I WAYAN DIKSA Gede Mekse Korri Arisena Gede Suastika Gede Suastika GEDE WIJANA GREGORY C. LUTHER Gunadi, Gusti Alit GUSTI AYU DWITA ANDRAWINA H. Yuswanti Hartha , I Komang Gede Suweca HERRY KUSUMA YUDHA HESTIN YUSWANTI I Dewa Gede Raka Sarjana I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I Gede Agus Adi Chandra I Gusti Agung Oka Hendrawati I GUSTI ALIT GUNADI I Gusti Alit Gunadi I GUSTI AYU ARI SANTIKADEWI I GUSTI AYU DEVI VALENIA SARI I GUSTI AYU KARISMAYATI I GUSTI NGURAH PRABU WIRA SANJAYA I GUSTI NGURAH RAKA I KADEK ARYARTHA I KETUT PURNA YASA I Ketut Siadi I KETUT SUMIARTHA I Komang Candra Giri Prayoga I Made Arimbawa I MADE DEDIK SETYADI I Made Sudana I MADE SUDANA I Made Sudana I Made Sudana I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I MADE SUPARTHA UTAMA I MADE WINANTARA I NENGAH ARTHA I NYOMAN DARMA YASA I NYOMAN RAI I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I Putu Sudiarta I PUTU WIRYA SUPUTRA I Wayan Diara I WAYAN RUSMAN I Wayan Susila I.G.A. Gunadi Ida Ayu Putri Darmawati Ida Bagus Gde, pranatayana JOKO MARIYONO K.A. Yuliadhi K.B. Susrusa KESUMADEWI, ANAK AGUNG ISTRI KETUT AYU YULIADHI Ketut Ayu Yuliadhi KETUT BUDI SUSRUSA Khairun Nisak Syahdu KHAMDAN KALIMI Khamdan Khalimi Klett, Katrina KOMANG ADI MAHARTHA Komang Adi Mahartha Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Satya Andrayuga Masahiro Shishido MEI NOVITA BR PARDEDE Mimi Sutrawati Muhammad Ikhsan Nulzaen N.N.A. Mayadewi NI KADEK NINA ARI SUCI NI KADEK SRI UTARI Ni Komang Budiyani Ni Luh Gde Sumardani NI LUH MADE INDAH MURDYANI DEWI Ni Luh Putu Citra Innosensia NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE INDRA PUSPAWATI Ni Made Intan Maulina Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE TRIGUNASIH NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ARI MAYADEWI NI NYOMAN DWI RESPITA NINGSIH Ni Putu Merthaningsih Ni Putu Pandawani NI PUTU RATIH SUDIARTINI NI WAYAN SUNITI Nyoman Bintang Kartika Sari Pradana, I Kadek Wira Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna Regina I. M BanoEt Retno Kawuri Rindang Dwiyani SANJAYA, I GUSTI NGURAH PRABU WIRA Sarjana, Dewa Gede Raka Selangga, Dewa Gede Wiryangga SHAH KANIGARA ASADDIARI SONIA ASHA HASARI SUGIARTA, DWI SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya TRI ASMIRA DAMAYANTI TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA UTAMA, I WAYAN EKA KARYA Wibisana, I Made Dicky Chandra WIDHIANTHINI WIDHIANTHINI Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wulandari, Ni Kadek Pingkan Y. Fitriani YUDHA, I KADEK WISMA Yuliadhi , Ketut Ayu Yuyun Fitriani