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Influence of Some Packages of Technology on Pests Development on Chili Plants in Highland Area I Putu Sudiarta; Luciana Delavega; Ni Nengah Darmiati; Gusti Ngurah Alit Susanta Wirya; I Ketut Sumiartha; I Made Supartha Utama
Journal of Sustainable Development Science Vol 1 No 1 (2019)
Publisher : Dwijendra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.819 KB) | DOI: 10.46650/jsds.1.1.791.8-14

Abstract

Chili is a type of plant that can grow in various types of area, in lowland and highland areas and also can adapt well at a temperature of 24-27 ˚C. The purpose of this research in order to know the influence and effectiveness of the packages technology of Trichoderma sp. mixed with compost, rain shelter and pesticides to control the development of pests in cayenne chili plants and long chili in the highland area. This research was conducted from September 2017 to February 2018, in Pancasari Village, Buleleng Regency, Bali. The study used a Factorial Randomized Block Design with two factors and four times replications. The first factor was chili type (cayenne chili and long chilli), and the second factor was the use of technologies, compost plus Trichoderma sp., rain shelter, and pesticide. The variable was observed in this research were population of aphis, percentage of fruit fly and yields. The results showed that all treatment technologies effectively suppressed pests development when compared with control. The result show that the damage population of the highest aphis pest was found in control about 194 of Aphids, and population of aphids on pesticide treatment were not found. The result show that damage percentage of the highest fruit fly pest was found in control treatment with the percentage of 76,55% while the lowest percentage was found in pecticide treatment. The rain shelter treatment showed the highest yields were reached 405,87 gr and the control treatment showed the lowest yields were reached 12,03 gr.
Efektivitas Trichoderma sp. dan Gliocladium sp. untuk Pengendalian Penyakit Busuk Batang (Fusarium Oxysporum Sp.) pada Tanaman Vanili (Vanilla Planifolia) MEI NOVITA BR PARDEDE; GUSTI NGURAH ALIT SUSANTA WIRYA; KHAMDAN KALIMI
Agrotrop : Journal on Agriculture Science Vol 12 No 1 (2022)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2022.v12.i01.p06

Abstract

Effectiveness of Trichoderma sp. and Gliocladium sp. For Controlling Stem Rot (Fusarium Oxysporum sp.) in Vanilla (Vanilla planifolia). Vanilla (Vanilla planifolia Andrews) is one of the industrial plants that have very high economic value as an export commodity. One of the main obstacles to increasing vanilla production is stem rot disease. Stem rot disease caused by Fusarium oxysporum f. sp. vanillae became one of the main obstacles faced. This research on the effectiveness of antagonistic microbes aims to obtain the best antagonist microbes in suppressing stem rot disease in vanilla plants. The experiment was carried out in vitro at the Laboratory of Plant Diseases at Udayana University and in vivo at the Experimental Garden Greenhouse. The experiment used a randomized block design with six replications and four treatments, namely Control (without antagonistic microbes), G (Gliocladium sp. + compost), T (Trichoderma sp. + compost), and GT (Gliocladium sp. and Trichoderma sp. + compost). The results showed that T (Trichoderma sp. + compost) and GT (Gliocladium sp. and Trichoderma sp. + compost) able inhibit stem rot disease with the best percentage of 10%.
Identification and Characterization of Soft Rot Bacterial Pathogens on Phalaenopsis Orchid in Bali I Putu Wirya Suputra; Gusti Ngurah Alit Susanta Wirya; Nyoman Bintang Kartika Sari; I Gede Rai Maya Temaja; Ni Luh Putu Citra Innosensia
CROPSAVER Vol 5, No 1 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i1.39284

Abstract

The moth orchid (Phalaenopsis sp.) is one of the most popular orchids due to the various colors with distinctive shapes of the flowers. Soft rot disease caused by Pectobacteriaceae (SRP) family is commonly found infected this plant. The infected orchid showed pale-colored to blackish slimy rot. This research was conducted in three locations namely Denpasar, Badung, and Karangasem, and resulted in 10 candidates for pathogenic bacteria. The pathogenicity test of the pathogen candidates was carried out by injecting bacterial suspension into the orchid leaf tissue with the result of 6 bacterial isolates showing soft rot symptoms. Moreover, two specific primers Dda1F-Dda1R and Pcc3F-Pcc3R for Dickeya spp. and Pectobacterium spp. consecutively were used for the PCR test. The electrophoresis result of the PCR product identified the bacteria isolated from infected plants as Dickeya spp. Dickeya spp. showed white to yellowish-white colony color, with convex and circular colony form on PDA medium.
First report of Banana bunchy top virus on heliconia (Heliconia spp.) in Bali, Indonesia I Made Arimbawa; Gusti Ngurah Alit Susanta Wirya; I Putu Sudiarta
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12277-82

Abstract

Banana bunchy top virus (BBTV) mostly infects Musa spp. which causes banana bunchy top disease (BBTD), the most devastating viral disease in banana cultivation all over the world. During field survey in Sekar Bumi Tropical Farm located in Kerta Village, 15 heliconia plant samples showed green streak on lamina leaves that similar to common BBTD symptoms were collected. Further research was then conducted to identify molecular characteristic of BBTD in heliconia showing green streak. Molecular identification was conducted using polymerase chain reaction (PCR). Specific primers to amplified DNA target were used in this study: mRep/F (5’-GCGTGAAACGCACAAAAGGCC-3’) and mRep/R (5’-GCATACGTTGTCAAACCTTCTCCTC-3’). The expected 240 bp fragment target of partial master replication (mRep) gene was successfully amplified from ten out of 15 samples. Sequence analysis confirmed that the symptomatic heliconia samples were infected with BBTV and falls into the same clade with BBTV from the Asian Group.
Distribution and molecular characterization of Squash mosaic virus on cucumber in Gianyar, Bali Listihani Listihani; Ni Putu Pandawani; Tri Asmira Damayanti; Mimi Sutrawati; Dewa Gede Wiryangga Selangga; Ketut Ayu Yuliadhi; Trisna Agung Phabiola; Gusti Ngurah Alit Susanta Wirya
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12248-54

Abstract

Squash mosaic virus (SqMV) has been reported to infect cucumber plants in Java and cause a decrease in fruit quality and quantity. Until now, there is no information regarding Sqmv infection in Cucurbitaceae in Bali. In a preliminary research conducted during 2020, we found mosaic symptoms in Gianyar, Bali. SqMV was inferred to be the possible cause of the virus-disease-like symptoms. The study aimed to determine the precence of SqMV in Gianyar, Bali and to obtain information related to its molecular character. This research method includes surveys, field observations, virus identification by RT-PCR, and DNA analysis. Severe disease incidence caused by SqMV was observed on cucumber cultivation in Gianyar that was in the range of 5.81–66.87%. PCR using specific primer for coat protein (CP) gene of SqMV was successfully amplified the DNA fragments of ± 582 bp on samples from Payangan, Tegallalang, Ubud, Sukawati, Blahbatuh, and Gianyar districts, except for samples from Tampaksiring. This indicates that the presence of SqMV is widespread in Java and Gianyar.The SqMV isolate from Bali had the highest nucleotide homology at 91.9–93.4% and amino acids 94.0–94.5 and was closely related to the Brazil isolate (KT923125) and had lower isolate homology than other countries (China, Trinidad, Spain, Japan, Arizona, Cekoslowakia, and Australia). It proved that those mosaic symptoms on cucumber is associated with SqMV infection. SqMV is classified as a quarantine organism of category A2, so it is necessary to prevent its spread to other areas.
THE EFFECTIVENESS OF RHIZOBACTERIA AS BIOPROTECTANTS TO MITIGATE FUSARIUM WILT DISEASE AND AS BIOSTIMULANTS TO IMPROVE THE GROWTH OF CHILI (Capsicum annuum) Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; I Putu Wirya Suputra; Ni Luh Putu Citra Innosensia
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p04

Abstract

The aims of this study were to examine the potential of Rhizobacteria as bioprotectants and biostimulants on chili. The potential as bioprotectants was tested by the ability of these Rhizobacteria to inhibit the growth of the pathogenic fungus Fusarium oxysporum f.sp. capsici in vitro. Moreover, as biostimulants was tested by the ability of these rhizobacteria to produce IAA compounds and their ability to increase the plant growth parameter of chili. Four rhizobacteria were challenged for antagonistic activity against F. oxysporum f.sp. capsici. Chili seeds in this experiment were soaked in four different Rhizobacteria suspension for 15 minutes. For control, seeds were soaked with sterile water instead of Rhizobacteria suspension. Results of this experiment showed that four isolates of Rhizobacteria showed strong inhibitory activity against F. oxysporum f.sp. capsici on PDA medium. Percentage of inhibitory activity varied from 77,33% to 89,79%. The application of four isolates recorded significantly increased the plant growth parameters of chili. The plant height, number of leaves, leaf area, root length, shoot dry mass, root dry mass, and chlorophyll content on treated plants significantly higher than those of untreated control plants according to the Duncan’s multiple range test (P<0.05%). These results indicate that the tested Rhizobacteria can be used as bioprotectants because they can inhibit the growth of F. oxysporum f.sp. capsici and simultaneously as a biostimulant for the ability to produce IAA compounds and stimulate the growth of chili.
Tingkat Kerentanan Berbagai Umur Tanaman Melon (Cucumis melo L.) terhadap Infeksi Potyvirus I KADEK ARYARTHA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Susceptibility Levels of Various Ages of Melon Plants (Cucumis melo L.) Against Potyvirus Infection Domestic melon production has not been able to fulfill the community. Pest disruption is one of the main causes of declining melon production so that the community's demand for melons is not fulfilled. Potyvirus is a virus that is often found in melon plants in Bali, but information about the development of this disease is very limited. This study aims to determine the effect of time on the rate of Potyvirus infection in melon (Cucumis melo L.) plants. The steps taken in this study were preparing plants, inoculating Potyvirus in plants, observing disease progression and conducting research in the laboratory using the RT-PCR (Reverse Transcription Polymerase Chain Reaction) test. The results showed that the younger the melon plants infected by Potyvirus, the faster the rate of infection and the more severe the symptoms caused. The rate of infection in plants treated with inoculation at the age of 2, 3, 4, 5, 6, 7 weeks after planting and without inoculation treatment was 0.96 respectively; 0.60; 0.53; 0.41; 0.30; 0.18 and 0. Melon plants with inoculation treatment of 2, 3 and 4 weeks after planting were very susceptible to Potyvirus infection, which showed signs of severe mosaicism and severe malformations. Melon plants with inoculation treatment 5, 6 and 7 weeks after planting were more resistant to Potyvirus infection, namely showing moderate mosaic symptoms and mild mosaicism, while plants that were not given inoculation treatment showed no symptoms. Detection of Potyvirus using the RT-PCR method using primers (CI-FOR / CI-REV) successfully amplified Potyvirus fragments in the presence of base bands measuring ±683 bp in samples A, B, C, D, E, and F.
Identifikasi Penyebab Penyakit Bercak Merah pada Tanaman Stroberi (Fragaria sp.) di Bali dan Potensi Pengendaliannya dengan Jamur Antagonis Secara In Vitro I GUSTI AYU ARI SANTIKADEWI; GUSTI NGURAH ALIT SUSANTA WIRYA; TRISNA AGUNG PHABIOLA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Pathogenic Fungi causes Red Leaf Spot on Strawberry Plants (Fragaria sp.) in Bali and Control Potential with Antagonist Fungi Strawberry (Fragaria sp.) is a subtropical fruit plant that was first discovered in Chile, America and has high economic value. Strawberry plants growing in Bali show symptoms of pathogenic fungi attack. Symptoms include red spots on the leaf surface. The purpose of this study was to identify pathogenic fungi that cause disease in strawberry plants in the center of strawberry cultivation in Bali. The activities carried out in this study were (1) sampling, (2) isolation of pathogenic fungi from symptomatic strawberry plant parts, (3) pathogenicity test, (4) morphological identification of pathogenic fungi, and (5) potential inhibition test of antagonistic fungi. against pathogenic fungi. This research was conducted in Pancasari Village, Buleleng Regency and Candi Kuning and Kembang Mertha Villages, Tabanan Regency and continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University, Denpasar. The results of this study succeeded in identifying Pestalotiopsis sp. as a pathogen causingred spot disease on strawberry leaves in Bali and the potential for biological control using antagonistic fungi in vitro showed that Gliocladium sp. and Trichoderma asperellum can suppress the growth of pathogenic fungi on strawberry plants by > 80%, Trichoderma viride up to > 90% and Trichoderma koningii and Trichoderma harzianum up to > 50%.
Identifikasi Penyebab Penyakit Bercak Daun Mycosphaerella pada Tanaman Stroberi (Fragaria sp.) di Bali dan Potensi Pengendaliannya dengan Jamur Antagonis secara In Vitro GUSTI AYU DWITA ANDRAWINA; GUSTI NGURAH ALIT SUSANTA WIRYA; NI WAYAN SUNITI; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Pathogenic Fungi causes Mycosphaerella Leaf Spot on Strawberry Plants (Fragaria sp.) in Bali and Control Potential with Antagonist Fungi Strawberry (Fragaria sp.) is a fruit cultivation plant that develops in Indonesia and has attractiveness and high economic value. Strawberry plants that grow at the center of strawberry production in Bali show symptoms of pathogenic fungus attack. Symptoms caused by the presence of brownish purple spots with a white center on the leaves. The purpose of this study was to identify pathogenic fungi that caused Mycosphaerella leaf spot disease on strawberry plants located at the center of strawberry production in Bali. The activities carried out in this study were (1) sampling and calculating the percentage of Mycosphaerella leaf spot disease on strawberry plants, (2) isolation of pathogenic fungi from symptomatic leaves of strawberry plants, (3) pathogenicity testing, (4) identification of pathogenic fungi, and (5) test the potential inhibition of antagonistic fungi against pathogenic fungi. This research was conducted in Candi Kuning and Br Kembang Merta Village, Tabanan Regency and Pancasari Village, Buleleng Regency, and continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University. The results of this study were successful in identifying Mycosphaerella fragariae as a pathogen causing leaf spot disease on strawberry plants in Bali and the potential for biological control by utilizing antagonistic fungi in vitro showed that Trichoderma viride, Trichoderma asperellum, Gliocladium sp. can suppress the growth of pathogenic fungi in strawberry plants of > 90%, Trichoderma harzianum and Trichoderma koningii up to >80%.
Metode Isolasi Jamur Patogen Serangga (Aschersonia placenta) Menggunakan Media Water Agar dan Potato Sucrose Agar NI MADE SAVITA RASJMAN; I PUTU SUDIARTA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Isolation Method of Insect Pathogenic Fungi (Aschersonia placenta) using Water Agar Media and Potato Sucrose Agar Bali Province is one of the provinces that is famous for its superior agricultural commodities. One of the leading agricultural commodities is citrus. The production of citrus fruits in Bali Province in 2020 is 4,903,341 quintals based on data from the Central Statistics Agency for Bali Province 2021. However, in some citrus-producing areas, citrus fruit production has decreased compared to the previous year. The decline in citrus fruit production is caused by several factors, one of which is the attack of whitefly pests. Whitefly pests can be controlled by utilizing the insect pathogenic fungus Aschersonia placenta. This study aims to determine the isolation method of insect pathogenic fungi Aschersonia placenta using water agar media and potato sucrose agar. The study began with sampling, isolated the fungus and identifying the fungus by morphological characteristics. The results of this study showed that isolation method of A. placenta using water agar media and potato sucrose agar effective for multiply the fungus.
Co-Authors Abd. Rasyid Syamsuri Agung, I Dewa Agung Putra Anak Agung Istri Kesumadewi ARIYANTA, I PUTU BAWA AStiningsih, Ana Agung Made Chiharu Hongo Damastra, Garda Bagus DEBBIE OKTAVIANI DEPARI Devi, Komang Saraswati Devi, Ni Luh Putu Hartika Sinta Devi, Putu Shinta Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Ngurah Suprapta Dharmadiatmika, I Made Agus Dinarkaya, Shah Mahapati DWI WIDANINGSIH Eka Wijayanti, Febri Emilia Simpllisiu Ake Wangge GARGITA, I WAYAN DIKSA Gede Mekse Korri Arisena Gede Suastika Gede Suastika GEDE WIJANA GREGORY C. LUTHER Gunadi, Gusti Alit GUSTI AYU DWITA ANDRAWINA H. Yuswanti Hartha , I Komang Gede Suweca HERRY KUSUMA YUDHA HESTIN YUSWANTI I Dewa Gede Raka Sarjana I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I Gede Agus Adi Chandra I Gusti Agung Oka Hendrawati I GUSTI ALIT GUNADI I Gusti Alit Gunadi I GUSTI AYU ARI SANTIKADEWI I GUSTI AYU DEVI VALENIA SARI I GUSTI AYU KARISMAYATI I GUSTI NGURAH PRABU WIRA SANJAYA I GUSTI NGURAH RAKA I KADEK ARYARTHA I KETUT PURNA YASA I Ketut Siadi I KETUT SUMIARTHA I Komang Candra Giri Prayoga I Made Arimbawa I MADE DEDIK SETYADI I Made Sudana I MADE SUDANA I Made Sudana I Made Sudana I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I MADE SUPARTHA UTAMA I MADE WINANTARA I NENGAH ARTHA I NYOMAN DARMA YASA I NYOMAN RAI I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I Putu Sudiarta I PUTU WIRYA SUPUTRA I Wayan Diara I WAYAN RUSMAN I Wayan Susila I.G.A. Gunadi Ida Ayu Putri Darmawati Ida Bagus Gde, pranatayana JOKO MARIYONO K.A. Yuliadhi K.B. Susrusa KESUMADEWI, ANAK AGUNG ISTRI KETUT AYU YULIADHI Ketut Ayu Yuliadhi KETUT BUDI SUSRUSA Khairun Nisak Syahdu KHAMDAN KALIMI Khamdan Khalimi Klett, Katrina KOMANG ADI MAHARTHA Komang Adi Mahartha Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Satya Andrayuga Masahiro Shishido MEI NOVITA BR PARDEDE Mimi Sutrawati Muhammad Ikhsan Nulzaen N.N.A. Mayadewi NI KADEK NINA ARI SUCI NI KADEK SRI UTARI Ni Komang Budiyani Ni Luh Gde Sumardani NI LUH MADE INDAH MURDYANI DEWI Ni Luh Putu Citra Innosensia NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE INDRA PUSPAWATI Ni Made Intan Maulina Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE TRIGUNASIH NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ARI MAYADEWI NI NYOMAN DWI RESPITA NINGSIH Ni Putu Merthaningsih Ni Putu Pandawani NI PUTU RATIH SUDIARTINI NI WAYAN SUNITI Nyoman Bintang Kartika Sari Pradana, I Kadek Wira Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna Regina I. M BanoEt Retno Kawuri Rindang Dwiyani SANJAYA, I GUSTI NGURAH PRABU WIRA Sarjana, Dewa Gede Raka Selangga, Dewa Gede Wiryangga SHAH KANIGARA ASADDIARI SONIA ASHA HASARI SUGIARTA, DWI SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya TRI ASMIRA DAMAYANTI TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA UTAMA, I WAYAN EKA KARYA Wibisana, I Made Dicky Chandra WIDHIANTHINI WIDHIANTHINI Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wulandari, Ni Kadek Pingkan Y. Fitriani YUDHA, I KADEK WISMA Yuliadhi , Ketut Ayu Yuyun Fitriani