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All Journal Microbiology Indonesia Indonesian Journal of Urology Jurnal Berkala Epidemiologi Indonesian Journal of Tropical and Infectious Disease Belitung Nursing Journal J-Dinamika: Jurnal Pengabdian Kepada Masyarakat JURNAL KESEHATAN MERCUSUAR Majalah Biomorfologi (Biomorphology Journal) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Jurnal Pengabdian Masyarakat dalam Kesehatan International Islamic Medical Journal Narra J Jurnal Ilmiah Kesehatan (JIKA) Makara Journal of Health Research
Juniastuti Juniastuti
Department Of Microbiology, Medical Faculty, Universitas Airlangga, Surabaya Indonesia-Japan Collaborative Research Center For Emerging And Re-emerging Infectious Diseases Institute Of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
Religion Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Microbiology Indonesia

 1 
Analyses of Precore and Core Promoter Mutations of Hepatitis B Virus in Patients with Chronic Hepatitis B in Surabaya, Indonesia . JUNIASTUTI; EDUARDUS BIMO AKSONO; TAKAKO UTSUMI; YOSHIHIKO YANO; . SOETJIPTO; YOSHITAKE HAYASHI; HAK HOTTA; FEDIK ABDUL RANTAM; HERNOMO ONTOSENO KUSUMOBROTO; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1048.865 KB) | DOI: 10.5454/mi.4.3.%p

Abstract

Mutations of precore (A1896) and core promoter (T1762/A1764) of hepatitis B virus can reduce HBeAg production. These mutations are frequently found in the late HBeAg seroconversion. However, it has been a controversy about the role played by precore and core promoter mutations in determining outcome of chronic hepatitis B. In the present study, the variability of precore and core promoter of hepatitis B virus were analyzed using PCR amplification and sequencing, according to the outcome (viral load and HBeAg/anti-HBe) in chronic hepatitis B patients in Surabaya. The study groups included 5 patients with uncomplicated chronic hepatitis B and 10 patients with chronic hepatitis B and liver cirrhosis in Dr. Soetomo Hospital, Surabaya. The control group included 6 blood donors obtained from Indonesia Red Cross, Surabaya. All groups were HBsAg positive. Precore mutation A1896 was predominant in all groups (60%-67% of each), together with precore variant T1858. As reported, precore variant T1858 is a prerequisite for precore A1896 and characteristic for viral genotype. Nevertheless, core promoter mutations T1762/A1764 were predominant only in LC patients (60%). All of these mutations were found mostly after HBeAg seroconversion (anti-HBe+). Of most samples with anti-HBe+, precore mutation was related with low viral load (<105 copies/mL), but core promoter mutations with high viral load (>105 copies/mL). Precore mutation A1896 was predominant in all groups, but core promoter mutations T1762/A1764 were only predominant in LC patients. The precore mutation alone is possible not critical to indicate a poor outcome, the core promoter mutations must be considered also.
The Prevalence and Subtype Distribution of Hepatitis C Virus Infection among Hemodialysis Patients in a Private Hospital in Surabaya, Indonesia MOCHAMAD AMIN; JUNIASTUTI .; TAKAKO UTSUMI; YOSHIHIKO YANO; MOCHAMAD YUSUF; MOCHAMMAD THAHA; PRIYO BUDI PURWONO; RETNO HANDAJANI; SOETJIPTO .; HAK HOTTA; YOSHITAKE HAYASHI; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.539 KB) | DOI: 10.5454/mi.6.4.5

Abstract

The prevalence of hepatitis C virus (HCV) infection has been as high as approximately 80% in patients with maintenance hemodialysis in public hospitals in Indonesia. However, the prevalence in private hospitals has not been examined yet. The aim of  this study was  to  investigate  the prevalence of anti-HCV antibody and  the subtype distribution in patients with hemodialysis in a private hospital in Surabaya, Indonesia. Sera were obtained from 41 hemodialysis patients in a private hospital in Surabaya. The positive prevalence of anti-HCV antibody was carried out by  the enzyme-linked  immunosorbent assay (ELISA). Anti-HCV-positive sera were subjected  to  reverse transcription-PCR (RT-PCR) to detect HCV RNA and then direct sequencing. The HCV subtype was examined by phylogenetic analysis. Twenty five patients (61%) out of 41 were positive for anti-HCV antibody, and HCV-RNA was detected in 19 patients. The positive prevalence of anti-HCV antibody was 7.7% (one out of 13 patients) among patients who had undergone hemodialysis  for  less  than one year, whereas  it was 85.7%  (24 out of  28  patients)  among  patients who  had  undergone  hemodialysis  for  over  one  year.  Phylogenetic  analysis revealed HCV-1a  (52.6%) was  the most common  subtype,  followed by 1b  (15.8%), 1c  (15.8%), 2a  (5.3%), and 3k (5.3%). In conclusion,  the prevalence of HCV  infection among hemodialysis patients  in a private hospital was as high as  that  in general hospitals. The predominant subtype was HCV-1a, which  is  in accordance with  the previous studies  in general hospitals  in Surabaya,  Indonesia.
Co-Authors A.A. Ketut Agung Cahyawan W Achmad Ardianto, Achmad Adhiningsih, Yunita Ratri Adiana Mutamsari Witaningrum Adnyana , I Made Dwi Mertha Ahmad Suryawan Aksono HP., Eduardus Bimo Al Karina, Citra Alfaray, Ricky I. Alimsardjono, Lindawati Alpha Fardah Athiyyah Ana Bilah, Aisah Nur Andy Darma Annisa Fira Salsabila Asmarawati, Tri Pudy Budi Utomo Deby Kusumaningrum Desi Fitriani Dewi, Selva R. Dian Marcia, Dian Doddy M. Soebadi Dominicus Husada Dwi Endraswari, Pepy Dwiyanti Puspitasari, Dwiyanti Edward M Putera, Edward M Eko Budi Koendhori, Eko Budi Faisal Faisal Fathmawati Fathmawati Fatimah Arief Fauzia, Kartika A. Fedik Abdul Rantam Fikri Sasongko Widyatama Firmansyah, Joedhistira Bayu Fitria, Anisa Lailatul Florentina Sustini HAK HOTTA HAK HOTTA Hana Heny Arwati Herman Kosasih HERNOMO ONTOSENO KUSUMOBROTO Hunowu, Ramdan I Gusti Made Reza Gunadi Ranuh Ilma Dzurriyyatan Toyyibah Itja Firdarini, Itja Khairul Bariyah, Khairul Laura Navika Yamani Lindarto, Wira Widjaya M. Qushay, M. Maharani, Aussie Tahta Mahmudatul Muna, Nafdzu Maria I Lusida, Maria I Maria Inge Lusida MARIA INGE LUSIDA MARIA INGE LUSIDA MOCHAMAD AMIN MOCHAMAD AMIN Mochamad Yusuf Alsagaff MOCHAMMAD THAHA Moses Tende Stephens Muhammad Faizi Muhammad Miftahussurur Muna, Nafdzu Makhmudatul Musofa Rusli Nasronudin Nasronudin Nur Rochmah Nurul Wiqoyah, Nurul Pepy Dwi Endraswari, Pepy Dwi Peter Chilaque Dossen Prihartini Widiyanti Priyo B Purwono, Priyo B Priyo Budi Purwono Rebekah Setiabudi, Rebekah Retno Handajani Retno Pudji Rahayu Retno Wahyunitisari, Manik Rusli , Musofa Sardjono, Lindawati Alim Septyawati, Ratna Setiawan, Firman Siti Qamariyah Khairunisa, Siti Qamariyah SOETJIPTO . SOETJIPTO . Sri Subekti Sudarmo, Subijanto Marto Sukmawati Basuki Sulistiawati Sunaryo Hardjowijoto Takako Utsumi TAKAKO UTSUMI TAKAKO UTSUMI Taufik Indrawan Tomohiro Kotaki, Tomohiro Vermasari, Naritha Wahyunitisari, Manik Retno Wiwin Retnowati YOSHIHIKO YANO YOSHIHIKO YANO Yoshio Yamaoka, Yoshio YOSHITAKE HAYASHI YOSHITAKE HAYASHI Yunita Ratri Adhiningsih Zayyin Dinana