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All Journal Buletin Peternakan Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Annales Bogorienses Berita Biologi

Atit Kanti

Unknown Affiliation

Author-ID : 295442

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Taxonomic Approach for Species Diversity of Yeasts and Yeasts-like Fungi through D1/D2 Region of Large Subunit Ribosomal DNA Sequences Sumerta, I Nyoman; Kanti, Atit
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11588

The identification of yeasts or yeasts-like fungi and verify their diversity are principal aspect for bioindustry and ecosystem sustainability. Taxonomic approach provides identification tool to ensure the taxonomic position of yeasts and yeasts-like fungi which definitely set to utilization concerns. The aim of this study is to understanding the taxonomic position of yeasts and yeasts-like fungi from the distinctive of its sequences relationship. Yeasts and yeasts-like fungi strains were isolated through various culture dependent methods from natural resources samples of Karimun Besar Island, Province of Riau Islands, Indonesia. The identification process was performed through amplifying the accurate DNA-based in D1/D2 region of large subunit (26S) ribosomal DNA. As the result,Â a total of 85 isolatesÂ of yeasts and yeasts-like fungi were obtained with 16 closest related taxa through phylogenetic tree construction. AscomycetousÂ was the predominating group representing 91% of the total isolates sequences followed byÂ BasidiomycetousÂ (8%) and Zygomycetous (1%). The black yeasts (yeasts-like) known as Aureobasidium melanogenumÂ was predominant species with represent to 54% of total isolates and present in particular habitat. Taxonomically, there are six isolates are represent to be novel taxa candidates which pretend to enhance genetic resources of yeasts and yeasts-like fungi especially from Indonesia. In addition, this information provides specific technique to reach specific yeasts or yeasts-like fungi species in nature by managing the sample collection and culture methods.

AKTIVITAS CMC-ase KHAMIR Candida sp. YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA, PAPUA Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i5.1190

Cellulolytic Candida sp.was isolated from Wamena, Papua.The strain was able to grow in media with carboxymethyl cellulose as a sole carbon source implying that the isolate produced 1-3 oc endo-gluconase.To study the effect of glucose and osmotic pressure on 0.1% glucose and 0.1% NaCl were augmented to CMC-contained media.Glucose augmentation affects cellulolytic activity of culture,probably due to higher biomass production in media. NaCl addition appear not to affect cellulolytic ability. Profile of pH also varied depend on the cultivation media. Maximum growth rate was achieved when glucose was added which was implying that glucose stimulate cell growth.

DIVERSITY AND ECOLOGICAL PERSPECTIVE OF SOIL YEAST IN GUNUNG HALIMUN NATIONAL PARK*[Khamir Tanah Taman Nasional Gunung Halimun: Keragaman dan Perspektif] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Taman Nasional Gunung Halimun merupakan hutan hujan tropika yang berada di pulau Jawa. Penelitian ini ditujukan untuk mengetahui populasi dari khamir tanah pada berbagai ketinggian tempat (600 m, 1000 m, dan 1500 m) di atas permukaan laut, dan selanjutnya mempelajari peran ekologi dari khamir, dilakukan dengan mengisolasi dan menganalisa karakter fisiologi terutama dalam mendekomposisi selulosa dan hidrolisa fosfat. Khamir yang diisolasi dari tanah dan daerah perakaran dimurnikan dan ditumbuhkan pada media yang mengandung carboxymethylcellulose (CMC) sebagai sumber karbon utama, dan juga ditumbuhkan pada media pivoskaya untuk mengetahui kemampuan pelarutan Ca3(PO4)2. Populasi khamir pada daerah yang atas sedikit lebih tinggi .Sebanyak 23 isolat khamir yang termasuk dalam marga Debaryomyces, Pichia, Rhodotorula, dan Candida diisolasi dari tanah.15 isolat mempunyai kemampuan menghidrolisa selulosa dan 9 isolat mampu melarutkan Ca3(PO4)2. Khamir ditemukan di tanah dan daerah perakaran dan memegang peran penting dalam degradasi senyawa organik dan mineralisasi fosfat di dalam tanah.

IDENTIFICATION OF YEASTS ISOLATED FROM GUNUNG HALIMUN NATIONAL PARK*[Identifikasi Khamir pada Taman Nasional Gunung Halimun] Kanti, Atit; Julistiono, Heddy; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Dua puluh sembilan isolat khamir diisolasi dari tanah Taman Nasional Gunung Halimun. Sumber isolat berasal dari batang pohon lapuk, akar lapuk yang diambil dari Gunung Botol, Cikaniki, dan Cipta Rasa untuk dipelajari aspek taksonominya. Berdasarkan atas karakter morfologi dan fisiologi, isolat-isolat tersebut digolongkan kepada kelompok ascomyceteous, basidiomyceteous dan imperfect khamir. Selanjutnya ketiga golongan tersebut dimasukkan ke dalam sepuluh kelompok (Kelompok 1 sampai X). Dari 29 isolat tersebut, 7 isolat dimasukkan ke dalam kelompok I diindentifikasi sebagai Debaryomyces hansenii, 6 dalam kelompok II sebagai Candida sp, 2 dalam kelompok III sebagai Pichia membranafaciens, 5 isolat dalam kelompok IV sebagai Candida galacta, 1 dalam kelompok V sebagai Candida sake, 4 dalam kelompok VI sebagai Cryptococcus humicolus, 1 dalam kelompok VII sebagai Rhodotorula minuta, 1 dalam kelompok VIII sebagai Candida sp, 1 dalam kelompok Candida sp, dan 1 dalam kelompok X dalam Candida sp. Macam sampel tampaknya tidak berpengaruh kepada keragaman jenis khamir seperti ditunjukkan oleh jenis yang sama diisolasi dari berbagai jenis sampel. Dari banyaknya jenis khamir yang diisolasi menunjukkan bahwa keragaman jenis khamir di Taman Nasional Gunung Halimun tergolong tinggi.

CELLULOLYTIC YEAST ISOLATED FROM SOIL GUNUNG HALIMUN NATIONAL PARK* [Khamir Selulotik yang Diisolasi dari Tanah Taman Nasional Gunung Halimun] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Beberapa khamir tanah yang lermasuk dalam marga Debaryomyces dan Candida diisolasi dari tanah Taman Nasional Gunung Halimun.Kemampuan selulotiknya diuji dcngan menumbuhkannya pada media yang mengandung carboxymethyl cellulose (CMC) sebagai sumber karbon utama. Pertumbuhan biomassa dan pH diikuti selama kultivasi. Pengamh penambahan glukosa terhadap aktivitas selulolitik juga dipelajari.Dari 23 isolat yang diuji, 19 termasuk khamir selulolitik, dan 1 isolat yang mempunyai kemampuan tertinggi dipelajari karakterisktik ezymatik sclulasenya. Penambahan glukosa menstimulasi pertumbuhan sel dan menyebabkan kebutuhan sel akan glukosa bertambah, dan sclanjutnya memacu sintesa ensim. Dari isolat-isolat yang diuji, isolat Debaryomyces S-6 mempunyai aktifitas paling tinggi yaitu sebesar 12 UI pada 96 jam inkubasi. Selama waktu kultivasi pH meningkat dari 6,5 menjadi 7. Adanya khamir yang menghidrolisa selulosa mengindikasikan khamir memegang peranan penting dalam transformasi bahan organik dalam tanah.

THE COMMUNITY OF SOIL YEASTS IN GUNUNG HALIMUN NATIONAL PARK Kanti, Atit; Sudiana, I Made; Julistiono, Heddy
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fifty-two isolates were isolated from Gunung Halimun National Park on the basis of morphological and some physiological characteristics.Those isolates were belonged to three groups namely, ascomycetous, basidiomycetous and imperfect yeasts.Rhodotolum sp.was only found in Ciptarasa site at 1500 m asl, ascomycetous yeasts was only isolated from deteoretic root in Gunung Botol site, while Candida sp.(small globose shaped cells) was only isolated from soil at 1800 m asl of Gunung Botol site. Type of plant species appeared has no effect on yeasts diversity as shown by similar yeasts diversity was observed at rhizosphere soil of three dominating plant (Schima waallichii, Castanopsis javanica and Altingia excelsa) at Cikaniki study site.

Cellulolytic Yeast Isolated From Raja Ampat Indonesia Kanti, Atit; Sukarno, Nampiah; Sukara, Endang; Darusman, Latifah K
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The objective of this study was to select and characterize three yeast isolates originating from soil ofÂ Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS) with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolatesÂ Sporobolomyces poonsookiae Y08RA07,Â Rhodosporidium paludigenum Y08RA29 and Cryptococcus flavescens Y08RA33were 1.40, 2.60 and 1.66 respectively. CMC-ase produced optimum at pH 8 at 37ÂºC by isolate Y08RA07, whereas for Y08RA29 andY08RA33 were at pH 6, at 28ÂºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf. Â Key words: Cellulolytic yeast, Raja Ampat, waste paper, bamboo leaf

Production of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa, and Rhizophus oryzae on Sargassum and Rice Bran Under Solid State Fermentation Kanti, Atit
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The objective of study was to produce amylase, cellulase and phytase on sargassum and rice bran on solidÂ state fermentation using Aspergillus niger, Neurospora crassa and Rhizophus oryzae. Media for solid stateÂ fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moistureÂ content on phytase, amilase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase andÂ cellulase were obtained after 4 days fermentation at 30Â°C, and initial moisture content was adjusted to 60%. TheÂ optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula forenzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively.Â At this formula highest phytase activity was obtained by Aspergillus niger, cellulase by Rhizopus oryzae, andÂ amylase by Neurospora crassa. Media composed of sargassum and rice bran can be used for phytase, amylaseÂ and cellulase production.

Increase of Citric Acid Production by Aspergillus niger InaCC F539 in Sorghum’s Juice Medium Amended with Methanol Kanti, Atit; Ilyas, Muhammad; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3733

ABSTRACTCitric acid demand increases steadily, and there is a need to increase productivity through selection of suitable carbon sources, and addition of substances that increase citric acids production rate. Methanol has been suggested to increase citric acid fermentation on high carbohydrate containing substances. The objective of the study was to evaluate the suitability of sweet sorghum juice for citric acids production and to verify the effect of methanol on citric acids production using Aspergillus niger InaCC F539 as inoculant. Sweet sorghum juice with the total initial reducing sugar of 11.5 % (w/v) was used as the sole carbon sources. To study the effect of total initial reducing sugar on citric acid production the initial reducing sugar was adjusted to the concentration of 30 to 75 g/L. Preliminary experiment was conducted to get the optimum methanol concentration that stimulate citric acid production. The optimum methanol concentration that stimulate citric acid production was 4% (v/v). Submerged fermentation was conducted as shake culture (125 rpm at 28 °C). Citric acids production was affected by total initial reducing sugar. Higher total initial reducing sugar produced higher citric acids. Maximum citric acid production was 18.96g/L on sweet sorghum juice with 75 g/L total initial reducing sugar. Methanol 4 % (v/v) increase citric acid production by 41.35 to 65.89 %. Juice of sweet sorghum was a good medium for citric acids production, and methanol stimulate and increase citric acid production. It is a good basis for exploring efficient and cost effective industrial scale citric acid production. Keywords: Citric acid, Methanol, Sweet sorghum, Aspergillus niger

Identification of Ectomycorrhiza-Associated Fungi and Their Ability in Phosphate Solubilization Mujahidah, Shofia; Sukarno, Nampiah; Kanti, Atit; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3741

ABSTRACTThe existence of Pinus sp. is very dependent on ectomycorrhizal (ECM) fungi. ECM fungi affect the growth of their hosts especially by increasing mineral availability and water intake. However ECM fungi is not the only one that plays a role in the growth of their host. There are many ECM-associated fungi which also have many important roles in the growth of the host. Helotiales which were isolated from the ECM of Pinus merkusii are known as the most member of root associated fungi. Three isolated Helotiales identified as Scytalidium sp., Helotiales sp., and Glutinomyces sp. by morphological and molecular identification based on ITS1, 5.8S rRNA, ITS2 DNAr region. All three isolates have the ability to solubilize phosphate. Compared with C. geophilum which already known as P solubilizing fungi, Glutinomyces solubilized 16.6 ppm P which is higher than C. geophilum which solubilized as much as 13.68 ppm in Pikosvkaya medium with glucose as carbon source and rock phosphate as phosphate source. Then followed by Scytalidum sp. and lastly Helotiales sp. Rock phosphate tend to harder to solubilize because its complex chemical form with other minerals. Keyword: ECM-associated fungi, Helotiales, phosphate solubilizing ability, Pinus merkusii

Co-Authors Ahmad Thontowi Ahmad Thontowi ANDO KATSUHIKO ANDRIA AGUSTA Anja Meryandini Ariyanti Oetari Arwan Sugiharto Bambang Prasetya Chandra, Nicholas Dwi CHIHARU NAKASHIMA Elsera Br Tarigan, Elsera Br Endang Kusdiyantini Endang Sukara Euis HERMIATI Evana Faradila Ayu, Near Putri Guswenrivo, Ikhsan Habibi, Moh Hafid, Lukman HEDDY JULISTIONO Heddy Julistiono Helbert Helbert I Made Sudiana I Made Sudiana Idris Idris Ikhwani, Azra Zahrah Nadirah Kamal, Andi Saptaji Kartika Dyah Palupi latifah K Darusman Latifah K. Darusman Latupapua, H.J.D. LATUPAPUA, H.J.D. Lestari, Agis Dwi lina marlina, lina Lutfi Nia Kholida Maman Rahmansyah Mia Kusmiati Miftahul Ilmi, Miftahul Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Mujahidah, Shofia Mujahidah, Shofia N Nurhayati Nampiah Sukarno Napitupulu, Toga P. Napitupulu, Toga Pangihitan Napitupulu, Toga Pangihotan Pandanwangi, Aisyah Amelia Pratama, Aulia Brellian Purbani, Debora Christin Putri, Ade Lia RASTI SARASWATI Raunsai, Marlin Megalestin Rosida Rosida Senlie Octaviana Sri Listiyowati Sudiana, I. Made Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Suprapedi Suprapedi Urip PERWITASARI Wellyzar Sjamsuridzal WIBOWO MANGUNWARDOYO Yantyati Widyastuti Yopi YULIASRI JAMAL Yuliasri Jamal

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