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All Journal Buletin Peternakan Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Annales Bogorienses Berita Biologi
Atit Kanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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SCREENING AND EVALUATION OF VARIOUS CARBON SOURCES ON THE ABILITY OF TRICHODERMA HARZIANUM INACC TO SOLUBILIZE INSOLUBLE PHOSPHATE Napitupulu, Toga P.; Ilyas, Muhamad; Kanti, Atit; Sudiana, I. Made
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3814

Abstract

ABSTRACTOur previous works revealed that Trichoderma harzianum InaCC (Indonesian Culture Collection) produced volatile organic compounds that inhibited the growth of Fusarium oxysporum. The objective of this study was to evaluate the ability of T. harzianum InaCC to solubilize insoluble phosphate and optimize the carbon sources condition. Screening was conducted to 10 isolates of T. harzianum on Pikovskaya?s broth in 100-mL conical flasks. The cultures were incubated at 30 ± 1°C for  7 days on a rotary shaker at 80 r/min. All  T. harzianum isolates were observed for the solubility of insoluble tricalcium phosphate with the highest ability was performed by T. harzianum InaCC F88. Using this isolate, the optimization of insoluble phosphate solubilization was conducted on modifed Pivoskaya medium in 500-mL conical flasks in similar screening incubation condition with applying of different combination of phopshate sources (tricalcium phosphate and rock phosphate) and carbon sources (dextrose, lactose, and sucrose). When tricalcium phosphate was substituted by rock phosphate, less soluble phosphate was produced. Glucose was the best carbon source used for solubilization of both tricalcium phosphate and rock phosphate, then followed by lactose and sucrose. Acidification of medium was not the major mechanism of phosphate solubilization by T. harzianum. Based on the ability of high phosphate solubilization, T. harzianum InaCC F88 is the most potential strain as a plant growth promoting fungus among the 10  isolates of T. harzianum Ina CC.  Key words:  biocontrol, Pikovskaya medium,  phosphate solubilization, Trichoderma harzianum
INCREASE OF CITRIC ACID PRODUCTION BY ASPERGILLUS NIGER INACC F539 IN SORGHUM’S JUICE MEDIUM AMENDED WITH METHANOL Kanti, Atit; Ilyas, Muhammad; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3733

Abstract

ABSTRACTCitric acid demand increases steadily, and there is a need to increase productivity through selection of suitable carbon sources, and addition of substances that increase citric acids production rate. Methanol has been suggested to increase citric acid fermentation on high carbohydrate containing substances. The objective of the study was to evaluate the suitability of sweet sorghum juice for citric acids production and to verify the effect of methanol on citric acids production using Aspergillus niger InaCC F539  as inoculant. Sweet sorghum juice with the total initial reducing sugar of 11.5 % (w/v) was used as the sole carbon sources. To study the effect of total initial reducing sugar on citric acid production the initial reducing sugar was adjusted to the concentration of  30 to 75 g/L. Preliminary experiment was conducted to get the optimum methanol concentration that stimulate citric acid production. The optimum methanol concentration that stimulate citric acid production was 4% (v/v). Submerged fermentation was conducted as shake culture (125 rpm at 28 °C).  Citric acids production was affected by total initial reducing sugar. Higher total initial reducing sugar produced higher citric acids. Maximum citric acid production was 18.96g/L on sweet sorghum juice with 75 g/L total initial reducing sugar. Methanol 4 % (v/v) increase citric acid production by 41.35 to 65.89 %. Juice of sweet sorghum was a good medium for citric acids production, and methanol stimulate and increase citric acid production. It is a good basis for exploring efficient and cost effective industrial scale citric acid production. Keywords: Citric acid, Methanol, Sweet sorghum, Aspergillus niger 
IDENTIFIKASI KERAGAMAN KHAMIR YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA KABUPATEN JAYAWIJAYA, PROPINSI PAPUA Kanti, Atit; Latupapua, H.J.D.
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3483

Abstract

ABSTRACTThe Identification of Yeasts Which Were Isolated from Soils in the Wamena Biological Garden, Jayawijaya Residence, Papua Province. The present study deals with the isolation and identification of yeasts isolated from soil samples in The Wamena Biological Garden, Papua. Microbial population in 11 samples were estimated by plate count method. The yeasts population were ranged from 30.0 x102 until 16.5 x106 cells/g soil. Fifteen isolates of yeasts were taxonomically studied. On the basis of morphological and physiological characteristics these isolates were belonging to two group namely ascomycetous-imperfect yeasts and basidiomycetous yeasts. Futher, they were separated into three group. Out of 15 isolates, eleven isolates were included in group I and identified into genus level as Cryptococcus, three in group II as Candida and one isolate in group III identified into species level as Cryptococcus albidus. Type of sample sources appeared has no effect on yeasts diversity as shown by similar yeast isolate was observed isolated from different sources.Key words : Ascomycetous-imperfect yeasts, basidiomycetous yeasts, The Wamena Biological Garden, Papua.
Produksi Xilitol Menggunakan Hidrolisat Tongkol Jagung (Zea mays) Oleh Meyerozyma caribbica InaCC Y67 Faradila Ayu, Near Putri; Nurhayati, N; Thontowi, Ahmad; Kusdiyantini, Endang; Kanti, Atit; Hermiati, Euis
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.71-77

Abstract

Xylitol (C5H12O5) is a non-carcinogenic polyalcoholic sugar. Xylitol is beneficial for diabetics because it can be metabolized without insulin. Corn cobs contain 30% xylose which can be fermented into xylitol by microorganisms. Xylitol can be produced by fermentation of xylose and few microorganisms. Meyerozyma caribbica is a yeast that has been proven to produce xylitol and inhibitor’s resistant. The aim of this research is to test the xylitol productivity by Meyerozyma caribbica InaCC Y67 using corn cobs hydrolyzate and the effect of the volume of fermentation media on xylitol productivity by Meyerozyma caribbica InaCC Y67. The method was carried out by culturing Meyerozyma caribbica InaCC Y67 as a starter on YPD media.  Fermentation using 100 mL Erlenmeyer with the variation of fermentation volume is 10 ml and 75 ml, agitation 175 rpm and 30 oC. Parameters were measured based on the dry weight of cells, xylose and xylitol. Data were analyzed using fermentation kinetics. The results of analysis showed that the higher xylitol production was found in the fermentation volume 75 ml with an efficiency value of 7,171%. The highest xylitol production was at the 48th hour with production value of 2.050 g/L. Results from research shows that Meyerozyma caribbica InaCC Y67 can produce xylitol with corn cobs hydrolyzate. The right volume of fermentation in the fermentation process can also increase the productivity of xylitol.
KESIAGAAN PAKAN PADA TERNAK SAPI SKALA KECIL SEBAGAI STRATEGI ADAPTASI TERHADAP PERUBAHAN IKLIM MELALUI PEMANFAATAN BIODIVERSITAS FLORA LOKAL Maman Rahmansyah; Arwan Sugiharto; Atit Kanti; I Made Sudiana
Buletin Peternakan Vol 37, No 2 (2013): BULETIN PETERNAKAN VOL. 37 (2) JUNI 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i2.2427

Abstract

The presence of local floral resources as forage is essential, especially for the farm survival in anticipaty the impact of the global climate change. The availability of forage in the dry land ecosystems depends on water supply. However, the climate change has caused the change on rainfall pattern including the initiation of the dry season. The problem should be anticipated by the local farmers’ readiness in providing sufficient feed and their ability in performing the excellent management during all seasons. In some areas of East Bali and Nusa Tenggara (West andEast), dry climate crisis 2004 led to feed scarcity. Therefore, this study was conducted in order to identify the potential forage resources in the arid land. The discussion is focused on the results of survey and secondary data collection. Based on the results, there were 22 plants that could be used as forage which were abundant during rainy season, whereas 15 others were able to be forage and silage during the transitional season and along the dry season. The results also showed that the introduction of silage fermentation technique was required as the way to preserve feed for the supply during the dry season. In this study the role of polymer compounds hydrolyzing microbes and the fermentation process by the GRAS (Generally Recognized as Safe) microbes preceeded the role of Lactobacillus plantarum were observed. The results of this study may become a reference in anticipating the global climate change impacts in dry land areas by the application of functional microbial technology in small local farms’ feed management activities.(Key words: Local floral resources, Dry land climate, Fermented feed, Lactobacillus plantarum)
PENAPISAN KHAMIR SELULOLITIK CRYPTOCOCCUS SP. YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA, JAYA WIJAYA, PROPINSI PAPUA Atit Kanti
Jurnal Biologi Udayana Vol XI, No 1
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cryptococcus sp. was isolated from Kebun Biologi Wamena, Papua. The isolate was able to grow in media with carboxymethyl cellulose as a sole carbon source implying that isolate produced 1-3 ? endo-glucanase. To study the effect of glucose and osmotic pressure, 0.1 % glucose and 0.1 % NaCl were amended into the medium containing CMC. Glucose significantly affected cellulolytic activity and biomass synthesis. At the beginning of cell cultivation glucose augmentation appear to slightly inhibit enzyme activity. Sodium chloride also significantly affected cellulolytic activity. Profile of pH varied dependent on cultivation media. Maximum growth of biomass was achieved after glucose addition, indicating that glucose stimulated cell growth.
Keragaman Jenis Jamur Endofit pada Pandan Wangi (Pandanus amarylifolius) dan Aktivitas Antijamur Metabolit yang Diproduksinya Yuliasri Jamal; Muhamad Ilyas; Atit Kanti; Andria Agusta
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2685

Abstract

The whole nine isolates of endophytic fungi have been obtained from leaves and roots of pandan wangi (Pandanus amarylifolius) collected from Bogor Botanical Garden. Identification of the fungi isolates were conducted based on their morphological characteristics, and revealed that one fungus belongs to the genus of Fusarium, one of Dreschlera, and 7 fungi isolates belong to Coelomycetes. The ethyl acetate extract of the fungi cultures in PDB and GYP media showed a diverse secondary metabolite profiles. Ethyl acetate extract derived from the culture of PWA-2 fungus in GYP medium showed an antifungal activity against Saccharomyces cerevisiae, but not to Fusarium oxysporum and Candida tropicalis. The GC-MS analysis of the active extract showed eugenol as an active component.
Ecological and Taxonomical Perspective of Yeasts in Indonesia WELLYZAR SJAMSURIDZAL; ARIYANTI OETARI; ATIT KANTI; RASTI SARASWATI; CHIHARU NAKASHIMA; YANTYATI WIDYASTUTI; ANDO KATSUHIKO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8632.042 KB) | DOI: 10.5454/mi.4.2.3

Abstract

In the course of ecological and taxonomical study of yeasts in Indonesia, a total of 2147 yeast isolates from 315 samples in the year 2003, 2005, 2006, and 2008 had been obtained from eight locations e.g. Liwa (Sumatera), Cibinong (Java), Cibodas (Java), Kutai (Kalimantan), Enrekang (Sulawesi), Pucak (Sulawesi), Gili and Kuta (Lombok), and Kupang (Timor). Leaves, flowers, litters, soils, epiphytic soils, insects and insect's nests were collected for yeasts isolation. Our molecular identification based on D1/D2 region of nuclear large-subunit rDNA and the internal transcribed spacer (ITS) regions sequence data on 525 representative isolates revealed that 306 isolates belong to 48 described species (18 genera) and 209 strains belong to 19 undescribed species (19 genera), and 10 isolates were discarded because of contamination. Based on their substrates, litter had the highest yeasts genera (19) followed by soils (18), flowers (10), leaves (6), epiphytic soils (4), and insects and insect's nests (4). Genera found on soils were also common on litters. Yeasts genera found on flowers and epiphytic soils were common on leaves and litters. The genera Aureobasidium, Cryptococcus, Pseudozyma, Rhodotorula and Sporidiobolus were found in all substrates. Based on their locations, Kutai had the highest number of genera (15) followed by Cibodas (10), Cibinong (10), Enrekang (10), Kupang (10), Pucak (9), Liwa (7), and Lombok (7). The genus Cryptococcus was found in all locations. Our study shed a light to detection of many new taxa of yeasts, 41% of yeasts found in this study represented novel taxa.
DIVERSITY AND ECOLOGICAL PERSPECTIVE OF SOIL YEAST IN GUNUNG HALIMUN NATIONAL PARK*[Khamir Tanah Taman Nasional Gunung Halimun: Keragaman dan Perspektif] Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1167

Abstract

Taman Nasional Gunung Halimun merupakan hutan hujan tropika yang berada di pulau Jawa. Penelitian ini ditujukan untuk mengetahui populasi dari khamir tanah pada berbagai ketinggian tempat (600 m, 1000 m, dan 1500 m) di atas permukaan laut, dan selanjutnya mempelajari peran ekologi dari khamir, dilakukan dengan mengisolasi dan menganalisa karakter fisiologi terutama dalam mendekomposisi selulosa dan hidrolisa fosfat. Khamir yang diisolasi dari tanah dan daerah perakaran dimurnikan dan ditumbuhkan pada media yang mengandung carboxymethylcellulose (CMC) sebagai sumber karbon utama, dan juga ditumbuhkan pada media pivoskaya untuk mengetahui kemampuan pelarutan Ca3(PO4)2. Populasi khamir pada daerah yang atas sedikit lebih tinggi .Sebanyak 23 isolat khamir yang termasuk dalam marga Debaryomyces, Pichia, Rhodotorula, dan Candida diisolasi dari tanah.15 isolat mempunyai kemampuan menghidrolisa selulosa dan 9 isolat mampu melarutkan Ca3(PO4)2. Khamir ditemukan di tanah dan daerah perakaran dan memegang peran penting dalam degradasi senyawa organik dan mineralisasi fosfat di dalam tanah.
IDENTIFICATION OF YEASTS ISOLATED FROM GUNUNG HALIMUN NATIONAL PARK*[Identifikasi Khamir pada Taman Nasional Gunung Halimun] Atit Kanti; Heddy Julistiono; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1177

Abstract

Dua puluh sembilan isolat khamir diisolasi dari tanah Taman Nasional Gunung Halimun. Sumber isolat berasal dari batang pohon lapuk, akar lapuk yang diambil dari Gunung Botol, Cikaniki, dan Cipta Rasa untuk dipelajari aspek taksonominya. Berdasarkan atas karakter morfologi dan fisiologi, isolat-isolat tersebut digolongkan kepada kelompok ascomyceteous, basidiomyceteous dan imperfect khamir. Selanjutnya ketiga golongan tersebut dimasukkan ke dalam sepuluh kelompok (Kelompok 1 sampai X). Dari 29 isolat tersebut, 7 isolat dimasukkan ke dalam kelompok I diindentifikasi sebagai Debaryomyces hansenii, 6 dalam kelompok II sebagai Candida sp, 2 dalam kelompok III sebagai Pichia membranafaciens, 5 isolat dalam kelompok IV sebagai Candida galacta, 1 dalam kelompok V sebagai Candida sake, 4 dalam kelompok VI sebagai Cryptococcus humicolus, 1 dalam kelompok VII sebagai Rhodotorula minuta, 1 dalam kelompok VIII sebagai Candida sp, 1 dalam kelompok Candida sp, dan 1 dalam kelompok X dalam Candida sp. Macam sampel tampaknya tidak berpengaruh kepada keragaman jenis khamir seperti ditunjukkan oleh jenis yang sama diisolasi dari berbagai jenis sampel. Dari banyaknya jenis khamir yang diisolasi menunjukkan bahwa keragaman jenis khamir di Taman Nasional Gunung Halimun tergolong tinggi.
Co-Authors Ahmad Thontowi Ahmad Thontowi ANDO KATSUHIKO ANDRIA AGUSTA Anja Meryandini Ariyanti Oetari Arwan Sugiharto Bambang Prasetya Chandra, Nicholas Dwi CHIHARU NAKASHIMA Elsera Br Tarigan, Elsera Br Endang Kusdiyantini Endang Sukara Euis HERMIATI Evana Faradila Ayu, Near Putri Guswenrivo, Ikhsan Habibi, Moh Hafid, Lukman Heddy Julistiono HEDDY JULISTIONO Helbert Helbert I Made Sudiana I Made Sudiana Idris Idris Ikhwani, Azra Zahrah Nadirah Kamal, Andi Saptaji Kartika Dyah Palupi latifah K Darusman Latifah K. Darusman LATUPAPUA, H.J.D. Latupapua, H.J.D. Lestari, Agis Dwi lina marlina, lina Lutfi Nia Kholida Maman Rahmansyah Mia Kusmiati Miftahul Ilmi, Miftahul Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Mujahidah, Shofia Mujahidah, Shofia N Nurhayati Nampiah Sukarno Napitupulu, Toga P. Napitupulu, Toga Pangihitan Napitupulu, Toga Pangihotan Pandanwangi, Aisyah Amelia Pratama, Aulia Brellian Purbani, Debora Christin Putri, Ade Lia RASTI SARASWATI Raunsai, Marlin Megalestin Rosida Rosida Senlie Octaviana Sri Listiyowati Sudiana, I. Made Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Suprapedi Suprapedi Urip PERWITASARI WELLYZAR SJAMSURIDZAL WIBOWO MANGUNWARDOYO Yantyati Widyastuti Yopi YULIASRI JAMAL Yuliasri Jamal