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PENGARUH METODE STERILISASI MEDIA NURSERI DAN ASOSIASI FUNGI MIKORIZA ARBUSKULAR TERHADAP PERTUMBUHAN TANAMAN CABAI (Capsicum annuum L.) Pratama, Aulia Brellian; Mangunwardoyo, Wibowo; Chandra, Nicholas Dwi; Napitupulu, Toga Pangihotan; Idris, Idris; Kanti, Atit; Ikhwani, Azra Zahrah Nadirah; Guswenrivo, Ikhsan; Sudiana, I Made
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.804

Abstract

Kondisi lingkungan yang kurang baik untuk industri pertanian cabai (Capsicum annuum L.) membuat hasil produksi cabai menurun. Pemberian inokulum mikoriza arbuskular dan pupuk dapat menjadi solusi untuk meningkatkan produksi cabai. Penelitian bertujuan untuk mengetahui pengaruh metode sterilisasi serta interaksi fungi mikoriza arbuskular terhadap pertumbuhan tanaman cabai. Metode penelitian dilakukan dengan melakukan penghitungan jumlah spora, sterilisasi media tanam, penyemaian benih, pengamatan karakteristik agronomi, hingga pengujian infeksi mikoriza. Spora mikoriza arbuskular yang digunakan berupa produk kultur tunggal Glomus mosseae (A) dan kultur campuran (B). Hasil penelitian menunjukkan penghitungan jumlah spora produk B memiliki jumlah spora tertinggi dengan 51 spora/50 g dibandingkan dengan produk A. Analisis karakteristik agronomi dengan ANOVA dua faktor (α: 0,05) menunjukkan tidak adanya pengaruh signifikan antara metode sterilisasi dengan pemberian inokulum mikoriza arbuskular pada tinggi tanaman cabai. Namun, hasil statistik menunjukkan bahwa pemberian mikoriza A dan B memberikan pengaruh terhadap tinggi tanaman cabai. Hasil analisis statistik pengukuran panjang akar juga tidak ada pengaruh signifikan antara pemberian mikoriza dengan metode sterilisasi. Pengamatan infeksi tanaman cabai menunjukkan fungi mikoriza arbuskular kultur tunggal dan campuran mampu menginfeksi akar dengan membentuk hifa internal dan spora. Hasil yang diperoleh dapat digunakan untuk menentukan media tanam serta inokulum mikoriza arbuskular terbaik untuk meningkatkan produksi cabai.
A CONTROL OF OXYGEN SUPPLY IN BIOCONVERSION OF SUGARCANE TRASH INTO XYLITOL BY Meyerozyma guilliermondii InaCCY65 Thontowi, Ahmad; Kanti, Atit; Listiyowati, Sri; Meryandini, Anja
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7720

Abstract

Xylitol is a sugar alcohol used as a sugar substitute for several prevention of health cases such as dental diseases, diabetes, and other health problems. Bioconversion of xylose into xylitol needs an optimum oxygen supply for xylitol synthesis. This research aims to determine the effect of dissolved oxygen on hydrolysate fermentation from sugar cane leaf as a source of xylose to xylitol by Meyerozyma guilliermondii InaCCY65. Dissolved oxygen was varied in aeration and fermentation agitation using a 3L scale bioreactor. Analysis of cell growth for several kinetic parameters during fermentation, xylose reductase, and xylitol dehydrogenase activity. Bioconversion of sugarcane trash hydrolysate into xylitol by M. guilliermondii InaCCY65 is influenced by the oxygen transfer coefficient (kLa) and aeration conditions. The increase in kLa number showed increased cell growth, xylose consumption, xylitol production, and decreased InaCCY65 cells. The optimum conditions of kLa were obtained at 45/h with 39 g/L xylitol production under the aeration effect. Optimum aeration in the bioconversion of sugarcane trash (SCT) hydrolysate become xylitol by M. guilliermondii InaCCY65 is 1.0%; under these conditions, xylitol yield and xylitol productivity are 0.78 g/g and 1.20 g/Lh. The effect of oxygen in the bioconversion of xylose to xylitol also has an impact on xylose reductase (XR) and xylose dehydrogenase (XDH) activities of M. guiellermondii InaCCY65. The results showed that the dissolved oxygen concentration must be carefully controlled during xylitol bioconversion to obtain efficient xylitol.
LIPID ACCUMULATION BY Flavodon flavus ATH USING PALM OIL MILL EFFLUENT AS SUBSTRATE Sudiana, I Made; Kanti, Atit; Helbert, Helbert; Octaviana, Senlie; Suprapedi, Suprapedi
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.989 KB) | DOI: 10.11598/btb.2014.21.2.388

Abstract

Large amount of palm mill effluent is generated annually. The waste would be potential for production of single cell oils (SCOs). The objective of study was to evaluate the lipid accumulation by fungi using palm mill effluent as substrate. To obtain most potential strains for lipid accumulation, seven filamentous fungi isolated from various biomes were evaluated for their ability to produce endoglucanase, and its lipid accumulation. Fungal hypae grown on palm oil mill effluent accumulated lipid of 34,3-87,5 of their dry cell mass. The profile of transesterified SCOs revealed a high content of saturated and monounsaturated fatty acids i.e., palmitic (C16:0), stearic (C18:0) and oleic (C18:1) acids similar to conventional vegetable oils used for biodiesel production. The strain was able to use organic substrates in POME implies that they are promising strain for biofuel feed stock as well as for meeting effluent quality for wastewater discharge.