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All Journal Buletin Peternakan Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Annales Bogorienses Berita Biologi
Atit Kanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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KERAGAMAN JENIS JAMUR ENDOFIT PADA PANDAN WANGI (PANDANUS AMARYLIFOLIUS) DAN AKTIVITAS ANTIJAMUR METABOLIT YANG DIPRODUKSINYA Jamal, Yuliasri; Ilyas, Muhamad; Kanti, Atit; Agusta, Andria
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.303 KB) | DOI: 10.24002/biota.v14i2.2685

Abstract

The whole nine isolates of endophytic fungi have been obtained from leaves and roots of pandan wangi (Pandanus amarylifolius) collected from Bogor Botanical Garden. Identification of the fungi isolates were conducted based on their morphological characteristics, and revealed that one fungus belongs to the genus of Fusarium, one of Dreschlera, and 7 fungi isolates belong to Coelomycetes. The ethyl acetate extract of the fungi cultures in PDB and GYP media showed a diverse secondary metabolite profiles. Ethyl acetate extract derived from the culture of PWA-2 fungus in GYP medium showed an antifungal activity against Saccharomyces cerevisiae, but not to Fusarium oxysporum and Candida tropicalis. The GC-MS analysis of the active extract showed eugenol as an active component.
THE PHSYIOLOGICAL CHARACTER OF BACTERIA ISOLATED FROM BANANA’S RHIZOSPHERE FROM MALAKA, EAST NUSA TENGGARA, AND THEIR ROLE ON PLANT GROWTH PROMOTION ON MARGINAL LAND Napitupulu, Toga Pangihotan; Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3748

Abstract

The objective of the study was to isolate microorganism that can produce growth hormone, fix atmospheric nitrogen, and solubilize inorganic phosphate that may be used for enhancing the growth of banana in marginal land.  A total of 25 bacteria associated with banana that belonged to six genera were isolated from namely Acinetobacter, Enterobacter, Pantoea, Klebsiella, Rhizobium, and Cupriavidus genera. Gammaproteobacteria is the dominant class, followed by Betaproteobacteria and Alphaproteobacteria. All isolates were screened for multiple plant growth promoting traits which may play a role in banana growth, namely fixing nitrogen, solubilizing phosphate, and producing Indole Acetic Acid (IAA). Twenty-two isolates were capable to fix nitrogen, 21 isolates can solubilize insoluble phosphate, and 15 isolates produced IAA dependent of L-Tryptophan presence. Despite most of the bacteria isolates exhibited one plant growth-promoting activities, Enterobacter and Klebsiella genera showed three of plant growth promoting bacteria traits. 
SCREENING AND EVALUATION OF VARIOUS CARBON SOURCES ON THE ABILITY OF TRICHODERMA HARZIANUM INACC TO SOLUBILIZE INSOLUBLE PHOSPHATE Napitupulu, Toga P.; Ilyas, Muhamad; Kanti, Atit; Sudiana, I. Made
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3814

Abstract

ABSTRACTOur previous works revealed that Trichoderma harzianum InaCC (Indonesian Culture Collection) produced volatile organic compounds that inhibited the growth of Fusarium oxysporum. The objective of this study was to evaluate the ability of T. harzianum InaCC to solubilize insoluble phosphate and optimize the carbon sources condition. Screening was conducted to 10 isolates of T. harzianum on Pikovskaya?s broth in 100-mL conical flasks. The cultures were incubated at 30 ± 1°C for  7 days on a rotary shaker at 80 r/min. All  T. harzianum isolates were observed for the solubility of insoluble tricalcium phosphate with the highest ability was performed by T. harzianum InaCC F88. Using this isolate, the optimization of insoluble phosphate solubilization was conducted on modifed Pivoskaya medium in 500-mL conical flasks in similar screening incubation condition with applying of different combination of phopshate sources (tricalcium phosphate and rock phosphate) and carbon sources (dextrose, lactose, and sucrose). When tricalcium phosphate was substituted by rock phosphate, less soluble phosphate was produced. Glucose was the best carbon source used for solubilization of both tricalcium phosphate and rock phosphate, then followed by lactose and sucrose. Acidification of medium was not the major mechanism of phosphate solubilization by T. harzianum. Based on the ability of high phosphate solubilization, T. harzianum InaCC F88 is the most potential strain as a plant growth promoting fungus among the 10  isolates of T. harzianum Ina CC.  Key words:  biocontrol, Pikovskaya medium,  phosphate solubilization, Trichoderma harzianum
ISOLATION AND IDENTIFICATION OF ACTINOMYCETES ASSOCIATED WITH MOSS ON THE SURFACE OF THE BOROBUDUR TEMPLE STONE Putri, Ade Lia; Purbani, Debora Christin; Kanti, Atit; Kusmiati, Mia; Habibi, Moh
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.20334

Abstract

Mosses growing on the surface of the Borobudur Temple will affect the aesthetic value of the temple. Interaction between moss and actinomycetes may trigger the growth of moss that can cause an increase in biodeterioration of stone. The purpose of this study was to isolate and identify the actinomycetes associated with moss on the surface of decayed stone of Borobudur Temple as well to assess their ability for phosphate solubilizing. Actinomycetes were isolated using serial dilution method and were identified based on 16S rRNA gene sequences. A total of 37 actinomycetes were isolated from three sampling sites. The isolates found belong to five genera (Gordonia, Microbacterium, Micromonospora, Nocardia, and Streptomyces) and distributed among four families (Microbacteriaceae, Micromonosporaceae, Nocardiaceae, and Streptomycetaceae). Isolates of actinomycetes composed of 19 Streptomyces Group and 18 Rare Actinomycetes Group. Nineteen isolates (51.35%) were identified as genus Streptomyces. Seventeen isolates (45.94%) showed abilities to release soluble phosphate and most of the isolates belong to the genus Streptomyces. The isolates have been collected will be deposited to Indonesian Culture Collection (InaCC) to enrich the collection of actinomycetes from ancient stone in Indonesia and will be used as a source of reference material research, taxonomic, or as source for further study.
PSEUDOZYMA APHIDIS AS INOCULANT FOR LOCAL CHICKEN Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2327

Abstract

SHORT COMMUNICATION
AKTIVITAS ENZIM SELULASE DARI KHAMIR CANDIDA SP. DAN DEBARYOMYCES SP. YANG DIISOLASI DARI LAHAN GAMBUT TAMAN NASIONAL BUKIT DUABELAS JAMBI Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i1.3273

Abstract

ABSTRACTCellulalytic Activity of Candida sp and Debaryomyces sp Isolated from Peat Soil ofBukit Duabelas Natural Park, Jambi. Candida sp. and Debaryomyces sp. YeastDegrading cellulose Isolated from Peat Soil in Bukit Duabelas National Park, Jambi. Theobjective of study was to investigate the characteristic of yeast that are able to solubilizecellulose. The yeast was isolated from soil of Tarnan Nasional Bukit Duabelas Jambi.Out of 2 1 ioslates tested 2 isolates Candida sp. (Isolate J I) and Debaryomyces sp. (IsolateJ2) were able to solubilize Carboxymethyl cellulose. Other isolated soil yeasts wereRhodotor& sp, and C~ptococcusp . Medium acidity during cell cultivation variedbetween 5.8 to 7.2. The CMC-ase activity was 5.9 unit and 5.4 unit for isolate J1 andisolate 52 respectively. The Km of isolate J1 and isolate 52 were 7.7 x 10" and 8.4 x 1 0-2(% b/v) respectively. Vmaks of isolate J1 and J2 was 8.28 xlW3 dan 30.66 x lo-) pgglukosa/ml enzyme /minute, respectively.Key words: Cellulolytic Yeasts, Candida sp., Debaryomyces sp., Enzymes Activity,Taman Nasional Bukit Duabelas
CARBOXYMETHYL CELLULOSE HYDROLYZING YEAST ISOLATED FROM SOUTH EAST SULAWESI, INDONESIA Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2203

Abstract

ABSTRACTThe objective of study was to isolate, identify and characterize the CMC-ase producing yeast from South East Sulawesi, Indonesia.  We isolated 142 strains and obtain 53 strains (37.32%) were CMC-ase producer consist of 26 species residing within 10 genera.  Candida was the most diverse genus consisting of 15 species. It is important to note that several strains residing within this genus could be candidate for new taxa, among others Candida aff. cylindracea PL2W1, Candida aff. insectorum PL3W6, Candida aff. friedrichii  MKL7W3, Candida aff. lessepsii, Candida aff. tenuis.  Five new candidates for novel species of cellulolytic yeast close to Yamadazyma mexicana: were Yamadazyma aff. mexicana (5 strains). Pichia, Pseudozyma, Sporodiobolus, and Sporobolomyces were other cellulolytic yeasts found in South East Sulawesi.  It is obvious, that leaf litter was a good source for cellulolytic yeasts. This CMC-ase producing yeasts dominate this biome, and production of extracellular cellulase is critical strategy for such yeast to survive in cellulose rich ecosystem such as leaf-litter.  This finding would suggest that yeasts play key role on hydrolyzes of cellulose and important resources for sustainable energy research. Keywords: cellulolytic yeasts, secondary forest, South East Sulawesi, leaf-litter 
EFFECT OF NITROGEN ADDITION ON THE ?-AMYLASE PRODUCTION BY ASPERGILLUS NIGER, RHIZOPUS OLIGOSPORUS AND NEUROSPORA CRASSA IN MEDIA CONTAINED SARGASSUM AND RICE SEED ON SOLID STATE FERMENTATION Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2891

Abstract

ABSTRACTAmylase is one of the hydrolytic enzymes which is commercially important and represent about 25?33% of the world enzyme market. Amylase production is affected by substrate for enzyme productions include particle size, initial moisture content and nutrient addition. The present study describe the effect of N-species addition on alpha amylase production by Aspergillus niger Km1, Rhizopus oligosporus Km2 and Neurospora crassa Km3 in medium contained sargasum and rice seed on solid state fermentation. Various ration of media composition contained dried sargassum and rice seed were studied. The effect of particle size of sargassum, initial water content on ?-amylase production were evaluated. The best media composition was then augmented with N-species include sodium nitrate, yeast extract, and peptone on solid state fermentation. Best media composition was 60:40 (w/w) of sargassum and rice seed respectively, with initial moisture content was 60%. Increase 5 times of amylase activity was obtained when sodium nitrate (0,5% w/w) was added to production medium. N-species significantly affect Alpha amylase production on sargassum and rice seed with maximum alpha amylase production was 36,66 unit by Rhizopus oligosporus.The economic value of Sargassum can be increased through alpha amilase production.Keywords: Amylase, Nitrogen, solid state fermentation, Rhizopus oligosporus, Neurospora crassa
KERAGAMAN JENIS KHAMIR PENGHASIL ETANOL YANG DIISOLASI DARI MAKANAN FERMENTASI DI KEPULAUAN RIAU Sumerta, I Nyoman; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3096

Abstract

ABSTRACTInformation on genetic diversity of fermentative yeast which produce ethanol is very crucial in developing biofuel production in Indonesia. Research on ethanol producing yeasts is interest of many scientist. The objective of study was to reveal yeast diversity in Indonesian fermented foods that able to produce ethanol. The sample of fermented foods were collected in the traditional market in Karimun Besar Island, Kepulauan Riau. Yeast isolation was performed using serial dilution with direct plating and enrichment culture with glucose as carbon source. Fifteen of isolates were isolated and identified by amplification of D1/D2 region LSU 26S rDNA. Its ethanol production characteristic was analyzed base on fermentation activity and measurement with gas chromatography for ethanol content. The result revealed that 8 yeast species were found belong to Ascomycetous and grouped into 5 clades which are able to produce ethanol. The highest ethanol production was obtained by Saccharomyces cerevisiae Y15Kr107 (3.53%) followed by Torulaspora delbrueckii Y15Kr104 (1.63%), Saccharomyces cerevisiae Y15Kr093 (1.58%), Candida glabrata Y15Kr110 (1.4%), Torulaspora delbrueckii Y15Kr103 (1.29%), Candida glabrata Y15Kr108 (1%), Torulaspora globosa Y15Kr094 (0.92%), Kodamaea ohmeri Y15Kr096 (0.61%), and Pichia kudriavsevii Y15Kr106 (0.31%) Y15Kr105 (0.21%) Y15Kr109 (0.16%). Other yeasts strains did not produce ethanol but may play different role in fermentation process.Key words: yeast, fermented food, ethanol, Kepulauan Riau
IDENTIFICATION OF ECTOMYCORRHIZA-ASSOCIATED FUNGI AND THEIR ABILITY IN PHOSPHATE SOLUBILIZATION Mujahidah, Shofia; Sukarno, Nampiah; Kanti, Atit; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3741

Abstract

ABSTRACTThe existence of Pinus sp. is very dependent on ectomycorrhizal (ECM) fungi. ECM fungi affect the growth of their hosts especially by increasing mineral availability and water intake. However ECM fungi is not the only one that plays a role in the growth of their host. There are many ECM-associated fungi which also have many important roles in the growth of the host. Helotiales which were isolated from the ECM of Pinus merkusii are known as the most member of root associated fungi. Three isolated Helotiales identified as Scytalidium sp., Helotiales sp., and Glutinomyces sp. by morphological and molecular identification based on ITS1, 5.8S rRNA, ITS2 DNAr region. All three isolates have the ability to solubilize phosphate. Compared with C. geophilum which already known as P solubilizing fungi, Glutinomyces solubilized 16.6 ppm P which is higher than C. geophilum which solubilized as much as 13.68 ppm in Pikosvkaya medium with glucose as carbon source and rock phosphate as phosphate source. Then followed by Scytalidum sp. and lastly Helotiales sp. Rock phosphate tend to harder to solubilize because its complex chemical form with other minerals. Keyword: ECM-associated fungi, Helotiales, phosphate solubilizing ability, Pinus merkusii 
Co-Authors Ahmad Thontowi Ahmad Thontowi ANDO KATSUHIKO ANDRIA AGUSTA Anja Meryandini Ariyanti Oetari Arwan Sugiharto Bambang Prasetya Chandra, Nicholas Dwi CHIHARU NAKASHIMA Elsera Br Tarigan, Elsera Br Endang Kusdiyantini Endang Sukara Euis HERMIATI Evana Faradila Ayu, Near Putri Guswenrivo, Ikhsan Habibi, Moh Hafid, Lukman Heddy Julistiono HEDDY JULISTIONO Helbert Helbert I Made Sudiana I Made Sudiana Idris Idris Ikhwani, Azra Zahrah Nadirah Kamal, Andi Saptaji Kartika Dyah Palupi latifah K Darusman Latifah K. Darusman LATUPAPUA, H.J.D. Latupapua, H.J.D. Lestari, Agis Dwi lina marlina, lina Lutfi Nia Kholida Maman Rahmansyah Mia Kusmiati Miftahul Ilmi, Miftahul Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Mujahidah, Shofia Mujahidah, Shofia N Nurhayati Nampiah Sukarno Napitupulu, Toga P. Napitupulu, Toga Pangihitan Napitupulu, Toga Pangihotan Pandanwangi, Aisyah Amelia Pratama, Aulia Brellian Purbani, Debora Christin Putri, Ade Lia RASTI SARASWATI Raunsai, Marlin Megalestin Rosida Rosida Senlie Octaviana Sri Listiyowati Sudiana, I. Made Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Suprapedi Suprapedi Urip PERWITASARI Wellyzar Sjamsuridzal WIBOWO MANGUNWARDOYO Yantyati Widyastuti Yopi YULIASRI JAMAL Yuliasri Jamal