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CELLULOLYTIC YEAST ISOLATED FROM SOIL GUNUNG HALIMUN NATIONAL PARK* [Khamir Selulotik yang Diisolasi dari Tanah Taman Nasional Gunung Halimun] Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1173

Abstract

Beberapa khamir tanah yang lermasuk dalam marga Debaryomyces dan Candida diisolasi dari tanah Taman Nasional Gunung Halimun.Kemampuan selulotiknya diuji dcngan menumbuhkannya pada media yang mengandung carboxymethyl cellulose (CMC) sebagai sumber karbon utama. Pertumbuhan biomassa dan pH diikuti selama kultivasi. Pengamh penambahan glukosa terhadap aktivitas selulolitik juga dipelajari.Dari 23 isolat yang diuji, 19 termasuk khamir selulolitik, dan 1 isolat yang mempunyai kemampuan tertinggi dipelajari karakterisktik ezymatik sclulasenya. Penambahan glukosa menstimulasi pertumbuhan sel dan menyebabkan kebutuhan sel akan glukosa bertambah, dan sclanjutnya memacu sintesa ensim. Dari isolat-isolat yang diuji, isolat Debaryomyces S-6 mempunyai aktifitas paling tinggi yaitu sebesar 12 UI pada 96 jam inkubasi. Selama waktu kultivasi pH meningkat dari 6,5 menjadi 7. Adanya khamir yang menghidrolisa selulosa mengindikasikan khamir memegang peranan penting dalam transformasi bahan organik dalam tanah.
THE COMMUNITY OF SOIL YEASTS IN GUNUNG HALIMUN NATIONAL PARK Atit Kanti; I Made Sudiana; Heddy Julistiono
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1081

Abstract

Fifty-two isolates were isolated from Gunung Halimun National Park on the basis of morphological and some physiological characteristics.Those isolates were belonged to three groups namely, ascomycetous, basidiomycetous and imperfect yeasts.Rhodotolum sp.was only found in Ciptarasa site at 1500 m asl, ascomycetous yeasts was only isolated from deteoretic root in Gunung Botol site, while Candida sp.(small globose shaped cells) was only isolated from soil at 1800 m asl of Gunung Botol site. Type of plant species appeared has no effect on yeasts diversity as shown by similar yeasts diversity was observed at rhizosphere soil of three dominating plant (Schima waallichii, Castanopsis javanica and Altingia excelsa) at Cikaniki study site.
AKTIVITAS CMC-ase KHAMIR Candida sp. YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA, PAPUA Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i5.1190

Abstract

Cellulolytic Candida sp.was isolated from Wamena, Papua.The strain was able to grow in media with carboxymethyl cellulose as a sole carbon source implying that the isolate produced 1-3 oc endo-gluconase.To study the effect of glucose and osmotic pressure on 0.1% glucose and 0.1% NaCl were augmented to CMC-contained media.Glucose augmentation affects cellulolytic activity of culture,probably due to higher biomass production in media. NaCl addition appear not to affect cellulolytic ability. Profile of pH also varied depend on the cultivation media. Maximum growth rate was achieved when glucose was added which was implying that glucose stimulate cell growth.
Effect of Sucrose Concentration on Microbiological, Physicochemical, Antioxidant Activity, and Organoleptic Characteristics of Salak Fruit Juice Beverage Fermented with Lactobacillus Plantarum InaCC B153 Pandanwangi, Aisyah Amelia; Rosida, Rosida; Sudiana, I Made; Napitupulu, Toga Pangihitan; Kanti, Atit
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 3 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i3.486

Abstract

Salak is a tropical fruit that has great potential to be made into a healthy fermented beverage. The carbohydrate content and bioactive compounds such as phenolics in salak provide benefits as a source of antioxidants. The fermentation process will produce a probiotic drink that is good for digestive health. The fermentation process involves Lactobacillus plantarum bacteria as probiotic agents. This study aims to determine the effect of sucrose concentration on the characteristics of salak fruit juice probiotic drinks. This study used a completely randomised design (CRD) factorial pattern with sucrose concentration factor (3%, 6%, 9% b/v). The data obtained were analysed using ANOVA followed by DMRT further test at the 5% level. The results showed a significant interaction (p≤0.05). Fermented salak fruit juice using 9% sucrose was the best treatment with characteristics of pH 3.80; total microbes 9.023 log CFU/ml; total acid 1,11%; total sugar 13,88%; viscosity 118,35 mPa.s; DPPH antioxidant activity 89.99%. The resulting organoleptic properties including colour, aroma, and taste, overall were 3.40 (moderately liked), 3.08 (moderately liked), 3.76 (moderately liked), and 3,48 (moderately liked).
Random Mutagenesis of Lipomyces maratuensis InaCC Y720 Using Commercial UV Lamp To Increase Lipid Production Lestari, Agis Dwi; Kanti, Atit; Ilmi, Miftahul
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.29300

Abstract

AbstractOleaginous yeasts are capable of accumulating high lipid concentration up to 20% of dry cell weight. High lipid content, a shorter life cycle, and similar a fatty acid composition to vegetable oils makes oleaginous yeast a potential lipid producer. Lipomyces maratuensis InaCC Y720 is a novel species isolated from Maratua Island, East Kalimantan, which has been reported as a potential yeast lipid producer. However, lipid productivity of the yeast is needed to be increased to make it suitable for an industrial scale. The aim of this study is to obtain potential mutant strains for the biodiesel industry. Random mutagenesis was applied by using commercial UV-C lamp on the strain which resulting in an 80% death rate after three hours irradiation. Subsequent treatment was carried out using cerulenin as a selection agent for mutans, yielding six mutant strains. Among these strains, mutant 1 produced the highest lipid production, with a lipid concentration of 0.072 g/L and a lipid percentage of 8.603%.  Nevertheless, when compared to the wild type, the lipid productivity of mutant 1 is low. Based on these results, the mutagenesis approach using commercial lamp UV-C has not obtained the expected mutants, so it is recommended to use different methods for future study.AbstrakKhamir oleaginous memiliki kemampuan dapat mengakumulasi lipid hingga 20% dari berat kering selnya. Tingginya kadar lipid yang diproduksi, siklus hidup yang pendek serta komposisi lipid yang mirip dengan minyak tumbuhan dapat menjadikan khamir sebagai alternatif penghasil lipid. Lipomyces maratuensis InaCC Y720 merupakan spesies baru yang diisolasi dari Pulau Maratua, Kalimantan Timur yang dilaporkan sebagai khamir penghasil lipid potensial. Namun, produktivitas lipid khamir tersebut perlu ditingkatkan agar sesuai untuk skala industri. Tujuan dalam studi ini adalah mendapatkan strain mutan yang potensial untuk industri biodiesel. Metode mutagenesis secara acak dilakukan dengan menggunakan lampu UV-C komersial pada strain yang menghasilkan tingkat kematian 80% selama tiga jam penyinaran. Setelah itu, dilakukan perlakuan lebih lanjut dengan penggunaan serulenin sebagai agen seleksi mutan. Proses seleksi menghasilkan enam strain mutan. Di antara keenam strain mutan, mutan 1 menghasilkan jumlah lipid tertinggi dengan berat lipid 0,072 g/L dengan persentase lipid yaitu 8,603%. Namun, dibandingkan dengan wild type, produktivitas lipid mutan 1 lebih rendah. Berdasarkan hasil ini, mutagenesis menggunakan lampu UV-C komersial belum mendapatkan mutan yang diharapkan sehingga disarankan penggunaan metode yang berbeda untuk penelitian selanjutnya. 
Effect of Sucrose Concentration on Microbiological, Physicochemical, Antioxidant Activity, and Organoleptic Characteristics of Salak Fruit Juice Beverage Fermented with Lactobacillus Plantarum InaCC B153 Pandanwangi, Aisyah Amelia; Rosida, Rosida; Sudiana, I Made; Napitupulu, Toga Pangihitan; Kanti, Atit
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 3 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i3.486

Abstract

Salak is a tropical fruit that has great potential to be made into a healthy fermented beverage. The carbohydrate content and bioactive compounds such as phenolics in salak provide benefits as a source of antioxidants. The fermentation process will produce a probiotic drink that is good for digestive health. The fermentation process involves Lactobacillus plantarum bacteria as probiotic agents. This study aims to determine the effect of sucrose concentration on the characteristics of salak fruit juice probiotic drinks. This study used a completely randomised design (CRD) factorial pattern with sucrose concentration factor (3%, 6%, 9% b/v). The data obtained were analysed using ANOVA followed by DMRT further test at the 5% level. The results showed a significant interaction (p?0.05). Fermented salak fruit juice using 9% sucrose was the best treatment with characteristics of pH 3.80; total microbes 9.023 log CFU/ml; total acid 1,11%; total sugar 13,88%; viscosity 118,35 mPa.s; DPPH antioxidant activity 89.99%. The resulting organoleptic properties including colour, aroma, and taste, overall were 3.40 (moderately liked), 3.08 (moderately liked), 3.76 (moderately liked), and 3,48 (moderately liked).
Furfural and 5-(Hydroxymethyl) furfural Tolerance Candida strains in Bioethanol Fermentation Ahmad Thontowi; Urip Perwitasari; Lutfi Nia Kholida; Atit Kanti; Yopi; Bambang Prasetya
Annales Bogorienses Vol. 24 No. 1 (2020): Annales Bogorienses
Publisher : BRIN

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Abstract

The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically well known. This study investigated the furfural and 5-HMF tolerance in Candida strains, which could be used for the development of advanced generation bioethanol processes. The 10 isolates of Candida were selected based on the cell growth and bioethanol production on YPD medium containing several concentrations of furfural and 5-HMF by using spectrophotometer and HPLC. Candida parapsilosis Y80 could grow and produce bioethanol in the medium that contains furfural and 5-HMF with a concentration of 60 mM and 40 mM, respectively. Based on the results, C. parapsilosis Y80 has potential activity in the development of bioethanol fermentation.
Cellulolytic Yeast Isolated From Raja Ampat Indonesia Kanti, Atit; Sukarno, Nampiah; Sukara, Endang; Darusman, Latifah K.
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

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Abstract

The objective of this study was to select and characterize three yeast isolates originating from soil of Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS)with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates Sporobolomyces poonsookiae Y08RA07, Rhodosporidium paludigenum Y08RA29, and Cryptococcus flavescens Y08RA33 were 1.40, 2.60 and 1.66, respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07; whereas for Y08RA29 and Y08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf. 
Production of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa, and Rhizophus oryzae on Sargassum and Rice Bran Under Solid State Fermentation Kanti, Atit
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
Publisher : BRIN

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Abstract

The objective of study was to produce amylase, cellulase and phytase on sargassum and rice bran on solid state fermentation using Aspergillus niger, Neurospora crassa, and Rhizophus oryzae. Media for solid state fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moisture content on phytase, amilase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase and cellulase were obtained after 4 days fermentation at 30°C, and initial moisture content was adjusted to 60%. The optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula forenzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively. At this formula highest phytase activity was obtained by Aspergillus niger, cellulase by Rhizopus oryzae, and amylase by Neurospora crassa. Media composed of sargassum and rice bran can be used for phytase, amylase and cellulase production.
BIODIVERSITY AND ANTIBACTERIAL ACTIVITIES OF ENDOPHYTIC FUNGI ASSOCIATED WITH UNCARIA GAMBIER (HUNTER) ROXB. VAR. UDANG FROM JASINGA, BOGOR, INDONESIA Palupi, Kartika Dyah; Kamal, Andi Saptaji; Marlina, Lina; Hafid, Lukman; Evana; Tarigan, Elsera Br; Raunsai, Marlin Megalestin; Ilyas, Muhammad; Kanti, Atit; Agusta, Andria
Berita Biologi Vol 22 No 2 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.713

Abstract

Endophytic fungi living in medicinal plant tissues are potential bioactive metabolite sources for drug discovery and development. However, the research regarding endophytic fungi associated with Indonesian medicinal plants is still limited. This study aimed to explore the biodiversity of endophytic fungi associated with the medicinal plant Uncaria gambir (Hunter) Roxb. var. udang and their antibacterial activities. Twenty-seven endophytic filamentous fungi were isolated from the surface-sterilized leaves, stems, and roots of Uncaria gambir (Hunter) Roxb. var. udang collected in Jasinga, Bogor, West Java, Indonesia. The observation based on the morphological characters both macroscopically and microscopically, as well as chemical profiling using thin layer chromatography (TLC), revealed that some of the isolated endophytic fungi were identical. Overall, the isolated endophytic fungi consist of six Coelomycetes, three Dematiaceae, four Pestalotiopsis sp., two Penicillium sp., one Aspergillus terreus, and two fungi were unidentified. Antibacterial activities of the ethyl acetate extract from the isolated endophytic fungi associated with Uncaria gambir (Hunter) Roxb. var. udang were observed against E. coli, B. subtilis, S. aureus, and M. luteus. Twelve types of isolated endophytic fungi from Uncaria gambir (Hunter) Roxb. var. udang exhibited antibacterial activities.