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Identification of Indigenous Bacteria from Dumai Sea Waters Using 16S rRNA Method Fatwa, Esa Buana; Yoswaty, Dessy; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 2 No. 3 (2021): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Indigenous bacteria are bacteria that naturally live freely in nature and have a variety of benefits for humans. These bacteria also have the potential to degrade pollution bacteria in the water. This research was conducted in November-December 2020. The purpose of this study was to and identify indigenous bacteria from dumai sea waters using the PCR method of 16S rRNA sequence technique. Based on the results of the study based on the analysis of 16S rRNA it is known that isolate 10 belongs to the bacterium genus Bacillus and has a homological rate of 93% with Bacillus paramycoides bacteria. Based on the phylogenic tree of isolate bacteria 10 does not have nodes associated with ten bacteria contained in NCBI. This suggests that phylogenically indigenous bacteria ISL 10 have no species similarity to the ten bacterial isolates in the phylogenetic tree, but have a similarity of base order of 93% to B. paramycoides
Analysis of Indigenous Bacteria as Microplastic Degradation of Sediment in the Sea Waters of Dumai, Riau Province Pakpahan, Deni; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 2 No. 3 (2021): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Nowdays Marine pollution resulted by microplastics is a global concern. Microplastic particles have the potential to cause damage to biota. One of solution to overcome the problem of marine pollution by microplastics by applying indigenous bacteria as microplastic degradation. This study was conducted to determine the type and ability of indigenous bacteria in sediments that are able to degrade microplastics. The research was conducted from October to December 2020. Sediment sampling was conducted in Dumai Sea Waters and sample analysis was conducted at the Marine Microbiology Laboratory, Faculty of Fisheries and Marine, Riau University. Indigenous bacteria from sediments were isolated in bacterial breeding media and then tested degradation capabilities on PET plastic samples. The result of the degradation test obtained ISL8 was able to degrade the mycoplastic with a percent degradation about 7%. Molecular test of ISL8 using PCR (Polymer chain reaction) 16S rRNA method and GenBank analysis is known that ISL8 is Bacillus sp.
Isolation and Identification of Bacteria from Dumai Marine Waters that Have Potencial as Lead Bioremediation Agents Yanti, Della Hijri; Nursyirwani, Nursyirwani; Yoswaty, Dessy
Journal of Coastal and Ocean Sciences Vol. 2 No. 3 (2021): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

One of the harmful pollutants to human health is the heavy metal Lead (Pb). A high concentration of lead that has can accumulate in the body of living things if in a long period of time. Lead concentrations that exceed the threshold in an environment can cause damage that affects aquatic biota and other living things. This research was carried out in February-April 2021. The purpose of this study was to isolate and identify bacteria that have the potential as lead bioremediation agents from Dumai marine waters. Isolation and identification of bacteria were carried out at the Laboratory of Marine Microbiology, Department of Marine Science, Faculty of Fisheries and Marine Sciences, and the lead concentration test at the Laboratory of Chemical Testing and Analysis, Department of Chemical Engineering, Faculty of Engineering. The results of the isolation on Marine Agar media which was added with Pb acetate (Pb(CH3COO2)) obtained three bacterial isolates from Dumai sea waters, namely D1, D2, and D3. The isolate that had the best growth was isolated D1. The isolate D1 was able to reduce the highest Pb concentration from 10 ppm by 80.53%, at a concentration of 20 ppm Pb, the isolate D1 was able to reduce Pb concentration by 78.8% and at a concentration of Pb 30 ppm, isolate D1 was able to reduce Pb concentration by 77.21%.
Antibacterial Activity of Avicennia alba Leaves and Stem Extracts Against Pathogenic Bacteria (Pseudomonas aeruginosa, Aeromonas salmonicida, Staphylococcus aureus) Purba, Paedo Yayaki; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 3 No. 2 (2022): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted in November 2020-February 2021 at the Laboratory of Fish Quarantine and Quality Control (BKIPM) Pekanbaru, Riau. The aim of this research was to test the activity of A. alba mangrove leaf extracts and stems in inhibiting the growth of pathogenic bacteria P.aeruginosa, A.salmonicida, and S.aureus. The research method used in this study is the experimental method. The sampling was conducted at the location of Marine Station UNRI Purnama Dumai. The results showed that A. alba leaf extract has the highest inhibitory power at a concentration of 100% with an average of 4.37–5.13 mm. The highest inhibitory power was against S.aureus (5.13 mm) and the lowest inhibition power was found in A. salmonicida (4.37 mm). The lowest inhibition was found at a concentration of 12.5% with an average of 0.45–0.67 mm. The stem extract of A. alba had the highest inhibitory power at a concentration of 100% with an average of 6.17–7.47 mm. The highest inhibition power was against S. aureus 7.47 mm and the lowest inhibition power was found in A.salmonicida (6.17 mm). The lowest inhibitory was at a concentration of 12.5% with an average of 1.52–2.29 mm. Based on the criteria for the strength of antibacterial activity of stem extract A. alba was in the medium category and the leaf extract was in the weak category
Identification of Phytochemical Compounds from Seaweed Extracts Eucheuma cottonii and Gracilaria verucossa and Their Effect on Bacteria Growth Escherichia coli Januardi, Deki; Mulyadi, Aras; Yoswaty, Dessy
Journal of Coastal and Ocean Sciences Vol. 4 No. 1 (2023): January
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Seaweed is known to have several bioactive compounds that function as secondary metabolites and antioxidants. This study aims to determine the compounds in the seaweed Eucheuma cottoni and Gracilaria verucossa and their effects on Escherichia coli bacteria. This research was conducted from February to April 2022. The research method used was the experimental method by performing phytochemical tests and the disc method on bacteria. Conclusions can be drawn because of the results of the research that has been done. Seaweed E.cottoni and G.verucossa contain steroidal compounds. The effect on the growth of E.coli bacteria at concentrations (5%) 8.24 mm, (10%) 8.5 mm, and (15%) 8.62 mm was classified as moderate
Analysis of Marine Ecotourism Suitability Based on Physical Environmental Conditions and Water Quality Index at Arta Sungai Limau Beach, Padang Pariaman Regency Livi, Aldisa Sipti; Mubarak, Mubarak; Yoswaty, Dessy
Journal of Coastal and Ocean Sciences Vol. 5 No. 3 (2024): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jocos.5.3.159-165

Abstract

Marine ecotourism is a type of tourism that involves activities related to marine activities carried out under or above the sea surface. Marine ecotourism generally covers three areas: the sea surface, under the sea, and on the coast. Marine ecotourism activities, especially beach tourism, are highly dependent on the condition of the oceanographic parameters of the region for the safety and comfort of tourists. This research was conducted in Arta Beach, Padang Pariaman Regency, West Sumatra Province, to determine the suitability of marine ecotourism at Arta Beach based on the parameters of physical environmental conditions. This study used a survey method: observation and data collection at the research location. Data analysis is used for the Tourism Suitability Index (IKW) method for physical environmental parameters and the STORET method for water quality parameters. The results of this study indicate that the tourism suitability index based on the physical condition of the environment carried out at Arta Beach at station 1 received a value of 2.49, including the suitable category, at station 2 received a value of 2.5, including the suitable category and station 3 received a value of 2.49 including the suitable category. The overall average value of IKW on Arta Beach is 2.49, included in the suitable category. This value means this beach can be used for beach ecotourism. Water quality parameters are analyzed using the storey method. The data obtained from the pH, DO salinity, and temperature parameters meet the quality requirements (good condition) and are suitable for marine tourism activities
TEST THE ABILITY OF SEDIMENT BACTERIA ISOLATES IN DEGRADATING PHENOL Monika, Siska; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Asian Journal of Aquatic Sciences Vol. 2 No. 1 (2019): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.914 KB)

Abstract

Phenol degrading bacteria can be found in various habitats in marine environments. This study aims to obtain bacteria from sediments that are able to degrade phenol. The process of bacterial purification and degradation was carried out from August to September 2018 at the Marine Microbiology Laboratory, Department of Marine Sciences, Faculty of Fisheries and Marine, University of Riau. Analysis of the reduction in phenol concentration was carried out using the APHA 5530 method using UV-VIS spectrophotometry conducted at the Health and Environment Laboratory. The bacterial isolates used as test bacteria were isolates BF1A, BF4B and BF9C. Bacterial and biochemical tests were carried out for all bacterial isolates. Two isolated showed mehyl red negative, all isolates were motile. Three isolates were positive catalase, able to ferment glucose and sucrose fermented citrate and two isolat were Gram negative bacterial. The three bacterial isolates were able to degrade phenol with the highest degradation for 1ppm shown in isolates BF1A, the highest degradation of concentrations of 2 ppm and 3 ppm was shown in isolates BF9C. Thus, the isolat BF9C was able to degrade the highest phenol
EFFECTIVITY OF EXTRACT LEAF, FRUIT, ROOT MANGROVE Avecennia marina ON Aedes aegypti Eriani, Eti; Effendi, Irwan; Yoswaty, Dessy
Asian Journal of Aquatic Sciences Vol. 2 No. 3 (2019): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (676.472 KB)

Abstract

Inhibiting the life of the Aedes aegypti mosquito is the mian way that is done to eradicate DBF, which is popularly done by chemically synthetic insecticides can be used air pollution and poisoning of humans so it needs safer natural incecticides such as compounds derived from mangrove plants. Mangrove Avecennia marina contains saponis, flavonoids, and alkaloids can act as stomach poisons resulting in larval death. The study was conducted in Februari-Maret 2019. The purpose of the study was to deteremine the efectiveness of extracts of leaves, fruit Avecennia marina mangrove root that quickly inhibit the growth of Aedes aegypyi larvae. The method used was the experimental method, the location of the research sample taking in Kayu Ara Village, Siak Riau Province. Results showed the mangrove leaves at a concentration of 12% mosquito larvae deaths reached 99 tails in the 120th minute, results showed the mangrove fruit at a concentration of 12% mosquito larvae deaths reached 96 tails in the 120th minute, Results showed the mangrove root at a concentration of 12% mosquito larvae deaths reached 85 tails in the 120th minute, analyzed using probit analysis to obtain LC50 (Lethal Concentration 50) and LT50 (Lethal Time 50) values. The LC50 probit analysis velue is estimate leaves 2.721, estimate fruit 1.584 dan estimate root 1,232. Whereas at LT50 estimate leaves 2.841, fruit 1.579, root 1.115.
SCREENING THE ANTIBACTERIAL POTENTIAL OF Avicennia marina MANGROVE LEAF EXTRACT AS A NATURAL ANTIBIOTIC INGREDIENT Sibarani, Michael Parluhutan Jupiter; Effendi, Irwan; Yoswaty, Dessy
Asian Journal of Aquatic Sciences Vol. 7 No. 3 (2024): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (760.204 KB) | DOI: 10.31258/ajoas.7.3.462-469

Abstract

Bacterial resistance to antibiotics is a global health problem caused by two factors, namely natural resistance and acquired resistance. Several factors can cause problems. The problem in this research is whether Avicennia marina mangrove leaf extract found in the mangrove ecosystem in Bandar Bakau, Pangkalan Sesai Village, Dumai City, can be used as an antibiotic. This research was conducted in January-March 2024. This research aimed to determine the potential of mangrove leaf extract A. marina as an antibiotic against pathogenic bacteria Vibrio sp, Aeromonas sp, and Pseudomonas sp. The results of this research will provide benefits in the form of information regarding the potential of A. marina mangrove leaf extract as an antibiotic. The methods used in this research are survey methods and experimental methods. The samples were then analyzed using the paper disk diffusion method at the Marine Microbiology Laboratory, Faculty of Fisheries and Marine Science, Universitas Riau. The data obtained is then presented and explained descriptively. The research results showed that antibiotics added to A. marina leaf extract could inhibit the growth of A.hydrophila bacteria based on the inhibition zone formed of 11mm. A.marina contains alkaloids, terpenoids, and flavonoids and has antibacterial properties against A.hydrophila bacteria. Based on the data, it can be concluded that A.marina leaf extract can be used against pathogenic bacteria
ANALYSIS OF TOTAL OIL CONTENT IN THE MARINE WATERS OF DUMAI CITY Hisbullah, Ali Akbar; Nedi, Syahril; Yoswaty, Dessy
Asian Journal of Aquatic Sciences Vol. 7 No. 3 (2024): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.7.3.450-455

Abstract

The Dumai City area is one of the regions currently designated as an industrial zone, a transportation route, and a busy international port. This situation puts significant pressure on the marine waters of Dumai City due to the waste produced. The primary source of marine pollution comes from oil spills, whether from ship operations, offshore drilling, or ship accidents. Pollution from oil spills in the ocean is a significant source of marine contamination that has consistently been a focus of public attention. This study aims to analyze the total oil content, Dissolved Oxygen (DO), Biological Oxygen Demand (BOD), and Total Organic Matter (TOM), as well as the effect of total oil content on DO, BOD, and TOM. The survey method used in this research involves collecting data from DO, BOD, TOM, and oil content measurements. The total oil content in Dumai City waters ranges from 0.14485 – 0.30214 ppm. The total oil content below the threshold still supports aquatic biota and does not damage the ecosystem. The DO content in Dumai waters ranges from 3.6 to 6.8 mg/L, and some measurements are below the threshold required to support marine biota. The BOD content of Dumai waters ranges from 10 to 11.3 mg/L, meaning that biological activity is still within optimal limits. The TOM content ranges from 17.38 – 35.22 mg/L, which does not support the life of aquatic biota, and there is potential for organic material pollution
Co-Authors ', Afrimansyah ', Frisilia ', Maryanto ', Mubarak ', Thamrin Abdurrahman, M. Adelina Adelina Afrimansyah ' Afrizal Tanjung Akbar, Aidil Rahmad Akhmad Ferdinan Hairo Alfiati Silfi Algino Panuntun Akbar Alit Hindri Yani Anak Agung Gede Sugianthara Andika Sitohang Angelia E Bernike Zebua Anggit Wondy Satrio Antonius ' Aras Mulyadi Arbaeyah ' Ardiah, Siti Arief Rachman. B Aris, Pajri Attur Mudzy Domo Azizul Fauzan Aznardi, Shodikin Batubara, Ummi Mardiah Bintal Amin Darina Darina Darma Bagus Saputra Darmawan, Ernanda P. Deni Efizon Devano, Rigo Devi Ambarwaty Oktavia Deviasari Deviasari Dewi, Shinta Rafida Dewita Dewita Domo, Attur Mudzy Efriyeldi, Efriyeldi Eka Saputra Elizal, Elizal Elkana Gabariel Eriani, Eti Eza Buana Fatwa Fahriansyah Fahriansyah Fatwa, Esa Buana Fatwa, Eza Buana Fauzan, Azizul Febby aprilliza Febri Naldi Feffiana M Amin Feliatra Feliatra, Feli Feri Handoyo Pardede Feronika Mendrofa Fitri, Sabrina Nur Gatot Yulianto Gustasya, Yosy Hade Mulyadi Hade Mulyadi Hanifa, Zurya Hapzi Ali Hari Winanda Hasyimi, Wahid Haura, Sari R. Al Hendra Kharisma Heni Pujiastuti HERI SYAHFITRI Herimufty Herimufty Hisbullah, Ali Akbar Hotdholy destrinanda Ida Rahayu Simanjuntak, Ida Rahayu Iesje Lukistyowati Ilbi Candra Yani Ilcia Teshi Diafana Ilham Ilahi Ilham Ilahi Ilman, Muhammad Khairi Indra Bayu Pratama Inka Wahyuni Irvina Nurachmi Irwan Efendi Irwan Efendi, Irwan Irwan Effendi Irwan Irwan Ismail Abdurrahman Siregar Januardi, Deki Joko Samiaji Jonny Zain Jung jung Maranata Juni Artha Hasibuan Kaprisal, Kaprisal Kelly Fuadi, Kelly Khairul Mukmin Lubis Kurnia, Fadly Agra Kurniawan, Mohd Rizky Kusdiatmoko, Aprizal Laksono Trisnantoro Livi, Aldisa Sipti Lucky Rahman Lukystyowati, Isye Mahdiyah, Evfi Mahmudah, Dewi A. Mardalisa, Mardalisa Matgus Riadi Mhd. Fadhillah, Mhd. Fadhillah Mirad, Awis Monika, Siska Mubarak Mubarak Mubarak Mubarak Muhammad Agus Muljanto Muhammad Alam Mulia Hasibuan Muhammad Hafizh Muhammad Razali MUHAMMAD RIZAL Musrifin Galib Musrifin Ghalib Naldi, Febri Niken Ayu Pamukas Nofri Andri Yulan Nursyirwani, Nursyirwani Oktarina, Ayu Oktavia, Dewi Asmiati Pajri Aris Pakpahan, Deni Panjaitan, Grace P Pardede, Feri Perie Anugraha Wiguna Prakasa Mustanto Prasetya, Dandy Purba, Paedo Yayaki Putra, Rajiv R. Ramadhan, Ragiel Ranap Junani Sari Rendy Lani Dinata Rifaldi Rifaldi, Rifaldi Rifardi Risma Tambunan Rival Junaidi Rizki, Fariza Rohadatul Aisy Romeo ' Romida, Zuwita Ronal Kurniawan Saharudin Saharudin Said Muhammad Qadry Saputri, Apriliana Saputri, Apriliana Eka Sari, Desy Mutia Sari, Nian Setiawan, Ilham Shodikin Aznardi Sibarani, Michael Parluhutan Jupiter Sihotang, Usia Xoros Sinuraya, Tri Setia Darma Siregar, Meiyanto Siringo-ringo, Irpan suriadi Siti Ardiah Siti Nursubadriah Situmorang, Adrian Sofyan Husein Siregar Sri Melinda Citra Syafruddin Nasution Syahada, Melania Syahril Nedi Syaifuddin Nasution T. Ersti Yulika Sari Tanjung, May Juna Tanjung, May Juna putri Thamrin ' Thamrin Thamrin Trisla Warningsih Ummi Mardiah Batubara Utami, Rahma I. Wahyuni, Inka Winanda, Hari Windarti Windarti Yanti, Della Hijri Yesi Yohana Esteria Yulinda, Emelda Yurestira, Ilham Yusni Ikhwan Siregar Zainuri -, Zainuri Zientika, Zientika Zulfan Saam Zulkarnaini Zulkarnaini Zulkifli ' Zulpikar '