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Bacterial Contamination at Whiteleg Shrimp (Litopeanaeus vannamei) in Aquaculture -, Mashuri Masri; Sukmawaty, Eka; Nur, Fatmawati; Suriani, Suriani
Jurnal Biodjati Vol 6 No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.11812

Abstract

Indonesia has a very high biodiversity, which has later become one of the natural products of interest to the international community, including fishery products. One of the high-demand Indonesian fishery products is whiteleg shrimp Litopeaneus vannamei. However, safety food Exported whiteleg shrimp products must meet the criteria, including free from bacterial contamination such as Escherichia coli, Salmonella, Vibrio cholera. This study attemptted to analyze E. coli, Salmonella, V. cholerae contamination in 3 ponds in Bojo, Cilellang, and Palanro Village in District Malusetasi, Barru Regency, South Sulawesi. Two samplings for each pond were conducted in the morning were pond water and  fresh whiteleg shrimp. SNI 2728-2018 specifies the quality and safety requirements for fresh shrimp. This standard applies to whole or headless fresh shrimp handled from fresh shrimp and does not apply to fresh shrimp that has undergone further processing. Based on SNI 2728-2018, the E. coli test showed positive in Cilellang Village (sample A) with 11 MPN/g, negative in Palanro Village (sample B) and in Bojo village (sample C) with the value of <2 MPN/g. Escherichia coli test showed positive in sample D (Vannamei shrimp in Cilellang Village) and sample E (Vannamei shrimp in Palanro Village) with 2.0 MPN/g, 17 MPN/g, respectively. Only sample F (Vannamei shrimp in Bojo village ) showed a negative result. As for the Salmonella test, positive results showed in sample A, while sample B and sample C showed negative results. The Vibrio cholerae test showed negative at all samples. . This study concludes that Whiteleg shrimp from ponds in Mallusetasi District is classified as safe for consumption.
Characterization of Soil Actinomycetes From Malino Pine Forest Rhizosphere of South Sulawesi Eka Sukmawaty; Sitti Rahmah Sari; Mashuri Masri
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.5383

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Abstract: Actinomycetes are a group of Gram-positive bacteria that produce active compounds with a wide distribution range in nature especially in soil. The purpose of this study was to isolate actinomycetes from the rhizosphere soil of the Malino pine forest, South Sulawesi. This research is a descriptive qualitative study of 15 actinomycetes isolated from the rhizosphere soil of the Pine Forest. These 15 isolates have been successfully identified to the genus level. Actinomycetes were isolated by direct dilution method and further morphological identification was carried out. Actinomycetes were isolated on yeast malt agar (YMA) medium. The growing isolates showed colony colors brown, gray, and white. Produces yellow, brown, and beige color pigments. Based on the characterization carried out, all isolates were identified into the genus Streptomyces.Abstrak: Aktinomisetes merupakan kelompok bakteri gram positif yang menghasilkan senyawa aktif dengan rentang distribusi yang luas di alam, terutama di tanah. Tujuan penelitian ini untuk mengisolasi aktinomisetes dari tanah rizosfer hutan pinus Malino Sulawesi Selatan. Penelitian ini merupakan penelitian kualitatif deskiptif terhadap 15 isolat aktinomisetes yang diisolasi dari tanah rizosfer Hutan Pinus Malino Sulawesi Selatan. 15 isolat ini telah berhasil didentifikasi sampai tingkat genus. Aktinomisetes diisolasi dengan metode pengenceran secara langsung dan dilakukan identifikasi morfologi lebih lanjut. Aktinomisetes diisolasi pada media yeast malt agar (YMA). Isolat yang tumbuh memperlihatkan warna koloni coklat, abu-abu dan putih. Menghasilkan pigmen warna kuning, coklat dan krem. Berdasarkan karakterisasi yang dilakukan keseluruhan isolat teridentifikasi ke dalam genus Streptomyces.
Comparison of Nata Quality From Cassava Peels (Manihot esculenta), Ladyfinger Bananas Peels (Musa acuminata Colla), and Durian Peels (Durio zibethinus) Mashuri Masri; Irhamniah Irhamniah; Ulfa Triyani A Latif; Rusny Rusny
Elkawnie Vol. 6 No. 1 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6870

Abstract

Abstract : Agricultural waste is composed of carbohydrates which can be be used as a growth medium for bacteria Acetobacter xylinum to produce fermented products in the form of nata.  Producing nata from fruit waste such as Cassava peels (Manihot esculenta), Ladyfinger bananas peels (Musa acuminata Colla) and Durian peels (Durio zibethinus) is one of the efforts to reduce environmental pollution. This study aims to compare the quality of nata from Cassava peels (endodermis), Ladyfinger bananas peels (endodermis) and durian peels (endodermis) based on the gel thickness, nata yield and organoleptic test. From the results of the study concluded that Durian peels and Ladyfinger bananas peels could be used as an essential ingredient in making nata. Nata de durio is the best nata product in terms of thickness, nata yield and organoleptic tests. Meanwhile Cassava peels cannot be used as raw material for making nata.Abstrak : Limbah pertanian terdiri dari karbohidrat, yang dapat digunakan sebagai media pertumbuhan bagi bakteri Acetobacter xylinum untuk menghasilkan produk fermentasi dalam bentuk nata. Memproduksi nata dari limbah buah seperti kulit ubi kayu (Manihot esculenta), kulit pisang Ladyfinger (Musa acuminata Colla), dan kulit Durian (Durio zibethinus) adalah salah satu upaya untuk mengurangi pencemaran lingkungan. Penelitian ini bertujuan untuk membandingkan kualitas nata dari kulit ubi kayu (endodermis), kulit pisang emas (endodermis), dan kulit durian (endodermis) berdasarkan ketebalan gel, rendemen nata, dan uji organoleptik. Hasil penelitian menyimpulkan bahwa kulit Durian dan kulit pisang emas dapat digunakan sebagai bahan penting dalam membuat nata. Nata de durio adalah produk nata terbaik dari segi ketebalan, rendeman, dan uji organoleptik, sedangkan kulit ubi kayu tidak dapat digunakan sebagai bahan baku pembuatan nata.
The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods) Opik Taupik Kurahman; Astri Yuliawati; Lusi Haerunnisa; Ateng Supriyatna; Tri Cahyanto; Yani Suryani; Asep Supriadin; Cecep Hidayat; Mashuri Masri
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.7770

Abstract

Abstract: In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar,  Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.
Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB Mashuri Masri; Cut Muthiadin; Masita Masita; Tri Cahyanto; Lianah Lianah; Rusny Rusny; Siska Tridesianti
Elkawnie Vol. 7 No. 1 (2021)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.9335

Abstract

Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of  N. sativa seed extract in inhibiting the growth of  M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan.  Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua  strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%,  tetapi resisten terhadap  ekstrak N. sativa konsentrasi 1 dan 3%.
The Potency of Bioplastic Polyhydroxyalkanoate (PHA) Producing Bacteria Isolated From Palm Oil Mill Waste Nur Haedar; Donny Suherman; Zasarwati Dwyana; Heriadi Heriadi; Mashuri Masri
Elkawnie Vol. 8 No. 1 (2022)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v8i1.11145

Abstract

Abstract:  Polyhydroxyalkanoate (PHA) is a polymer made up of biodegradable plastics that can be synthesized by microorganisms from various substrates that contain lots of carbon sources including fatty acids and sugars. One of the substrates that contain a lot of carbon sources is waste from palm oil processing plants. PHA-producing bacteria can take advantage of excess carbon sources in palm oil waste in the form of fatty acids to be converted into PHA. This study aims to determine the potential of bacteria isolated from palm oil mill waste to produce polyhydroxyalkanoates (PHA) and to determine the optimum time required for bacteria to produce PHA. Optimization of the fermentation time was carried out at 24, 48, and 72 hours using minimum Ramsay media added with 1% palm oil and 1% glucose. The results of the study obtained 20 isolates of bacteria isolated from palm oil mill waste, and 9 of them were able to produce polyhydroxyalkanoate (PHA) qualitatively. The results of quantitative selection obtained 2 bacterial isolates capable of producing PHA, namely isolates CPS 3 and LPS2 CPS 3, with crotonic acid absorbance values of 41.6 and 5.01, respectively with a fermentation time of 72 hours. Based on the results of genotypic identification using 16S rRNA DNA sequences, isolates of CPS 3, including Bacillus sp. strain CL33 and isolate LPS 2, belonged to Bacillus flexus strain S5a. This shows that isolates from palm oil mill waste can be used as a source of isolate for PHA production.Abstrak: Polyhydroxyalkanoate (PHA) merupakan polimer penyusun plastik biodegredable yang dapat di sintesis oleh mikroorganisme dari bermacam-macam substrat yang banyak mengandung sumber karbon asam lemak dan gula. Salah satu subtrat yang banyak mengandung sumber karbon adalah limbah yang berasal dari pabrik pengolahan kelapa sawit. Bakteri penghasil PHA dapat memanfaatkan sumber karbon berlebih dalam limbah kelapa sawit berupa asam-asam lemak untuk diubah menjadi PHA. Penelitian ini bertujuan untuk mengetahui potensi bakteri yang diisolasi dari limbah pabrik kelapa sawit dalam menghasilkan Polyhydroxyalkanoate (PHA) serta mengetahui waktu optimum yang dibutuhkan oleh bakteri dalam menghasilkan PHA. Optimasi waktu fermentasi dilakukan pada 24, 48 dan 72 jam menggunakan media minimal Ramsay yang ditambahkan minyak sawit 1% dan glukosa 1%. Hasil penelitian memperoleh 20 isolat bakteri yang diisolasi dari limbah pabrik kelapa sawit dan 9 isolat diantaranya mampu menghasilkan Polyhydroxyalkanoate (PHA) secara kualitatif. Hasil seleksi secara kuantitatif diperoleh 2 isolat yang mampu menghasilkan PHA yaitu isolat CPS 3 dengan nilai absorbansi asam krotonoat sebesar 41,6 sedangkan LPS 2 memiliki nilai absorbansi asam krotonoat sebesar 5,01 dengan waktu fermentasi selama 72 jam. Berdasarkan hasil identifikasi secara genotipik dengan menggunakan sekuens DNA 16S rRNA isolat CPS 3 termasuk jenis Bacillus sp. strain CL33 dan isolat LPS 2 termasuk jenis Bacillus flexus strain S5a. Hal ini menunjukkan bahwa isolat dari limbah pabrik kelapa sawit dapat digunakan sebagai sumber isolat untuk produksi PHA.
Molecular Identification of 16S RNA of Pathogenic Bacteria From The Wings of Flesh Flies (Sarcophaga sp.) Inspired by The Hadith of The Prophet SAW, Bukhari’s History About Flies Mashuri masri; Hajrah Hajrah; Asfiana Asfiana; Rusny Rusny; Syafrina sari Lubis
Elkawnie Vol. 11 No. 2 (2025)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v11i2.14962

Abstract

Abstract: Meat flies (Sarcophaga sp.) are one of the vectors of infection. Meat flies often fly by using contact wings with legs so that the fly’s flying area is very wide. This study uses qualitative and exploratory methods aimed at knowing the types of bacteria that can be found on the wings of meat flies (Sarcophaga sp.) through molecular identification. Samples were taken from the body parts of the right and left wings of meat flies which were coded (B1) left wing of fly 1; (A2) right wing of fly 2; (B2) left wing of fly 2; (A3) right wing of fly 3; (B3) left wing of fly 3; (A4) right wing of fly 4; (B4) left wing of fly 4; (A5) right wing of fly 5; (B5) left wing of fly 5. Meat fly wings (Sarcophaga sp.) were inoculated on BHIB day NA medium, and molecular identification day was done using 16S RNA. Based on molecular data, pathogenic bacteria were found in sample A5: Bacillus cereus, B. anthracis, and B. tropicus; and in sample B5, Moellerella wisconsensis was found. This research can provide a biological explanation of the hadith of the Prophet Muhammad (peace be upon him) about flies, indicating the presence of pathogenic bacteria on the wings of flesh flies (Sarcophaga sp.). Abstrak: Lalat daging (Sarcophaga sp.) merupakan salah satu vektor berbagai penyakit infeksi. Lalat daging banyak bergerak dengan menggunakan sayap dibandingkan dengan kaki, sehingga area terbang lalat sangat luas. Penelitian ini menggunakan metode kualitatif dan eksploratif yang bertujuan untuk mengetahui jenis bakteri yang terdapat pada sayap lalat daging (Sarcophaga sp.) melalui identifikasi molekuler. Sampel diambil dari bagian tubuh lalat daging sayap kanan dan kiri yang diberi kode (B1) sayap kiri lalat 1; (A2) sayap kanan lalat 2; (B2) sayap kiri lalat 2; (A3) sayap kanan lalat 3; (B3) sayap kiri lalat 3; (A4) sayap kanan lalat 4; (B4) sayap kiri lalat 4; (A5) sayap kanan lalat 5; (B5) sayap kiri lalat 5. Sayap lalat daging (Sarcophaga sp.) diinokulasi pada medium BHIB dan NA, dan identifikasi molekuler menggunakan 16S RNA.  Berdasarkan data molekuler ditemukan bakteri patogen pada sampel A5, yaitu Bacillus cereus, B. anthracis, dan B. Tropicus; pada sampel B5 ditemukan Moellerella wisconsensis. Hasil penelitian ini dapat membuktikan kebenaran hadits Rasululloh SAW tentang lalat dari perspektif biologis dengan membuktikan adanya bakteri patogen yang ditemukan pada sayap lalat daging (Sarcophaga sp.).
Co-Authors A, Suci Ananda A. Mustika Abidin Abdul Chadjib Halik Ahmad Ali Ahyar Ahmad Altriana Eka Putri Andiani Andiani Andiani Arfan Arifuddin Arifuddin Arifuddin As Awalia Amir Asbar Hamzah Asep Supriadin, Asep Asfiana Asfiana Asmuddin Natsir Asnilawati Asnilawati Astati Ateng Supriyatna AYU LESTARI Basir Paly Cecep Hidayat Cut Muthiadin Delima Engga Maretha Delima Engga Maretha Dirhamzah, Dirhamzah Donny Suherman Dwi Fitri Yani Ekafadly Jusuf Elmika Nesti Fatmawati Nur Fifi Dismayanti Indriani Nainu Hafsah Hafsah Hafsan Hajrah Hajrah Handayani Handayani Hardiyanti Hamrullah Hartati H, Hartati Hartati Hartati Heriadi Heriadi Ibrahim Ibrahim Ilham, Alya Salsabila Indah Islamiah Inna Shintia Irhamniah Irhamniah Irwan Irwan Isna Rasdianah Aziz Joko Widodo Khaerul Anwar Kurahman, Opik Taupik Laily Agustina, Laily Latif, Ulfa Triyani A. Lianah Lianah, Lianah Lili Silvana Lusi Haerunnisa Maghfirah M Marcia Bunga Pabendon Maretha, Delima Engga Masita Masita Maslan, Muhammad Masriany, Masriany Muhammad Arsan Jamili Muhammad Basri Muhammad Khalifah Mustami Muhammad Nur Hidayat Muhammad, Rusny Murtiadi Awaluddin Nur Haedar Nurhidayah N NURHIDAYAH NURHIDAYAH Nurhidayani Nurhidayani Nurlailah Mappanganro Nurul Afriani Arif NURZAKIYAH NURZAKIYAH Ramlah Ramlah Ramlah Rodiyati Azrianingsih Rosmah, Rosmah Rukmana, Rusmadi Rusny Rusny Rusny Rusny Sappewali Sappewali Sarah Shakina Silvana, Lili Sinar Wahyuni Siska Tri Desianti Siska Tridesianti Siti Patima Sitti Rahmah Sari Sjamsiah, Sjamsiah SRI UTAMI PUTRI Sukmawaty, Eka Suriani Suriani Suriani Suriani Susanti, Handayani Indah Syafaat, M. Aidin Syafrina sari Lubis Syahdar Baba Syahriani Syahriani, Syahriani Tang Silmy Zahrah Tri Cahyanto Tridesianti, Siska Ulfa Nur Alfriani Amir Ulfa Triyani A Latif Uswatul Hasanah Uswatul Hasanah, Uswatul Wahyuni Wahyuni Warobi Warobi Yani Suryani Yani, Dwi Fitri Yuliawati, Astri Yusminah Hala Zasarwati Dwyana