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ISOLAI DAN PENGUKURAN AKTIVITAS ENZIM BROMELIN DARI EKSTRAK KASAR BONGGOL NANAS (Ananas comosus) PADA VARIASI SUHU DAN pH Mashuri Masri
BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan Vol 2, No 1 (2013): BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.101 KB) | DOI: 10.33477/bs.v2i1.149

Abstract

Genetic Diversity of Local Maize Germplasm of Tana Toraja South Sulawesi Using SSR (Simple Sequence Repeat) Markers Ramlah Ramlah; Isna Rasdianah Aziz; Cut Muthiadin; Mashuri Masri; Muhammad Khalifah Mustami; Marcia Bunga Pabendon
Jurnal Ilmu Pertanian Vol 2, No 3 (2017): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.33085

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Plant genetic diversity is an emerging variation in a crop group caused by its genetic factors. Local corn germplasm as a source of plant genes that are able to adapt to the local environment. The purpose of this research is to obtain information on genetic variation of Tana Toraja local maize germ plasm using SSR (Simple Sequence Repeat) marker. This research was conducted at Balitsereal Molecular Biology Laboratory, Agricultural Research Agency in Maros Regency, South Sulawesi. A total of 4 local maize populations were analyzed by laboratory experimental method with observation with NTSYS pc 2.1 program. The results showed that the average number of alleles was 3.72 alleles per locus and the polymorphism rate of 0.53 with the genetic similarity coefficient was in the range of 0.47 to 0.85. 2 main clusters formed in the genetic similarity coefficient 0.47. Klaster I is Local DallePondan and Local Purple. Klaster II is Local Bebo and Kandora. The genetic distance is in the range of 0.15 to 0.74 with an average genetic distance of 0.46. From the data obtained shows that the 4th germplasm of the population of Tana Toraja Local maize diteleti has a very informative level of genetic diversity. Genetic diversity of local maize germplasm of Tana Toraja, can be used as a source of genes in the assembly of improved varieties in the future.
IDENTIFIKASI PROTEIN DARI CRUDE ANTIGEN OUTER MEMBRANE PROTEIN (OMP) SALMONELLA ENTERICA SEROVAR TYPHI ASAL SUSPEK DEMAM TIFOID MAKASSAR Asbar Hamzah; Cut Muthiadin; Mashuri Masri
Jurnal Biotek Vol 3 No 1 (2015): PROSIDING SEMINAR NASIONAL 22 Desember 2015
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7248.401 KB) | DOI: 10.24252/jb.v3i1.1896

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Penelitian ini bertujuan untuk mengetahui bobot molekul protein OMP S. Typhiasal Makassar dengan menggunakan metode SDS-PAGE. Penelitian ini dilakukanpada bulan Mei sampai Juni 2014 di Laboratorium Imunologi dan BiologiMolekuler Fakultas Kedokteran. Penelitian ini menggunakan antigen OMP hasilekstraksi kultur bakteri S. Typhi dari serum darah penderita demam tifoid yangdiperoleh dari Rumah Sakit di Kota Makassar. Monitoring bobot molekuldikerjakan menggunakan SDS-PAGE metode Laemmli yang telah dimodifikasiyaitu separating gel 12%, stacking gel 3%, dan pewarnaan gel (staining)menggunakan coomassie blue. Proses elektroforesis menggunakan teganganlistrik 120 volt 40 mA selama 3,5 jam. Gel hasil SDS-PAGE memperlihatkanadanya tiga band protein mayor OMP S. Thyphi yaitu pada 25 kda, 36 kda, 55kda, dikarenakan pada ekstrak kasar masih banyak debris dari protein lain
MAP OF EDIBLE ARACEAE BASED ON ABIOTIC FACTORS IN GOWA REGENCY, SOUTH SULAWESI Hafsah Hafsah; Rodiyati Azrianingsih; Mashuri Masri
Journal of Environmental Engineering and Sustainable Technology Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.255 KB) | DOI: 10.21776/ub.jeest.2018.005.02.2

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This study aims to create a distribution map and find out the relationship between the distribution of edible Araceae and abiotic factors in Gowa Regency, South Sulawesi. Edible Araceae explorations were carried out in 18 sub-districts in November 2016 to September 2018. Edible Araceae which was found to be marked with GPS location, documented with a camera, and measured abiotic factors including altitude, air temperature, soil temperature, light intensity, soil pH, humidity soil, air humidity and soil organic matter. The types of Araceae found included 9 species from 4 genera, namely Xanthosoma sagittifolium (L.) Schott., Xanthosoma nigrum, Colocasia esculenta (L.) Schott., Colocasia esculenta, Alocasia calidora, Alocasia macrorrhiza (L.) Schott. Amorphophallus muelleri Blume, Amorphophallus paeoniifolius, Amorphophallus variabilis Blume. Distribution of edible Araceae in Gowa Regency, South Sulawesi includes coastal areas (<100 m asl), lowlands (100-400 m asl) and highlands (<400 m asl). The distribution of Araceae which is affected by light intensity is Xanthosoma and Alocasia, while the distribution of Colocasia is influenced by altitude, soil organic matter, and soil moisture and Amorphophallus is influenced by soil pH. Araceae conservation strategy compiled based on species data, habitat preference, utilization and distribution of Araceae is expected to be used for the establishment of community business activities for the management of Araceae in each village that has an Araceae distribution.
Analisis Populasi Ternak Sapi di Kabupaten Bulukumba, Provinsi Sulawesi Selatan, Indonesia Rusny Rusny; Muhammad Basir Paly; Andiani Andiani Andiani; Mashuri Masri; Siska Tridesianti
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 8 No 1 (2022): Jurnal Ilmu dan Teknologi Peternakan Indonesia
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v8i1.112

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This study aims to analyze the development of beef cattle population, as well as to identify government efforts in order to develop beef cattle population in Kajang District. The study was carried out from February – August 2021 which is located in Kajang District, Bulukumba Regency, South Sulawesi Province. Quantitative data were analyzed using trend analysis, with the results of the study that there is a development of beef cattle population in Kajang District, Bulukumba Regency. For the period 2021 and 2022 shows that have been made by the development will continue to occur in the following year.
The effect of exposure to liquid air freshener on histological of the bronchial mice (Mus musculus) Warobi Warobi; Delima Engga Maretha; Asnilawati Asnilawati; Mashuri Masri
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 6 No 02 (2021): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, IKIP Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v6i02.1219

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Air fresheners are products that contain chemicals aimed at reducing unpleasant odours in confined spaces. The use of synthetic air fresheners turns out to harm health because some of their leased Volatile Organic Compounds are classified as toxic compounds and are carcinogens. Air freshener enters the body through the inhalation process in the respiratory system. Modern air fresheners are available in liquid (aerosol) and gel forms. Air fresheners contain addictive substances and solvents such as 1,4-dichlorobenzene which can affect pulmonary function. The purpose of this study is to know the effect of exposure to liquid air freshener on the histology of bronchi of mice (Mus musculus). This study used 20 male mice (Mus musculus) consisting of 4 treatments with 5 replications. The research design is true experimental in the form of a post-test only control group with Completely Randomized Design (CRD). The post-test was done by observing the histological picture of the mice's bronchi after exposure to liquid air freshener given 3x/day for a period of P1 = 2 weeks, P2 = 4 weeks and P3 = 6 weeks. Quantitative data on bronchial histology was tested using the One Way ANOVA statistical test followed by the Post Hoc Tukey test. The results of the study found changes in the histology of the bronchi, thickening of the epithelial tissue of mice. Analysis of comparative data between the control and treatment groups statistically obtained p = 0.010 <0.05, meaning that there was a significant effect and change in the bronchial tubes exposed to liquid air freshener. Histologically there were differences in histology between the control and treatment groups. It was concluded that liquid air freshener had a significant effect on the histological picture of the mice's bronchi.
THE ANTIBIOTIC RESISTANCE AND THE ABILITY OF BACTERIATO PRODUCE -LACTAMASES Mashuri Masri; Joko Widodo; Ekafadly Jusuf; Delima Engga Maretha; Wahyuni Wahyuni
Prosiding Seminar Nasional Biotik Vol 10, No 2 (2022): PROSIDING SEMINAR NASIONAL BIOTIK X 2022
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh, Aceh, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.435 KB) | DOI: 10.22373/pbio.v10i2.15165

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Background : Accessibility of bacterial patterns and their sensitivity to pus can be utilized as a thought in giving anti-microbials observationally. Vale Sorowako Inco Hospital was located in mining region where health services were not as enormous as in huge cities but can still carry out free sensitivity tests without send them to a referral hospital. Methods: Antibiotics (Penicillin, cefuroxime, Ceftriaxone, and meropenem) were tried by dise diffusion. The suspension of the test microscopic organisms was included with NaCL 0.85% until it reaches turbidity, at that point a stick sterile cotton swab of bacterial suspension on the MHA (Muller Hinton Agar) media,  paper disks containing the antibiotics were put on the media and incubated for 24 hours. Results: Staphylococcus aureus, Enterobacter cloacae, Enterobacter aerogenes, Acinetobacter baumannii, Pseudomonas aeruginosa, Serratia odorifera, and Aeromonas hidropila were bacteria isolated from pus. S. aureus and E. cloacae were the most (25%).  Meropenen is the most sensitive antibiotics  (90%), Penicillin and Cefuroxime are the most resistance (45%). Conclusions : The resistance that happens at Vale Sorowako Inco Hospital is not due to a prescription without a culture test ask, since antibiotics given by clinicians are continuously based on a culture test, but since of the capacity of bacteria to produce b-lactamases and the the presence of genes that can code for b-lactamases which moreover cause bacteria to resistant to antibiotics.
The Potency of Bioplastic Polyhydroxyalkanoate (PHA) Producing Bacteria Isolated From Palm Oil Mill Waste Nur Haedar; Donny Suherman; Zasarwati Dwyana; Heriadi Heriadi; Mashuri Masri
Elkawnie: Journal of Islamic Science and Technology Vol 8, No 1 (2022)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v8i1.11145

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Abstract:  Polyhydroxyalkanoate (PHA) is a polymer made up of biodegradable plastics that can be synthesized by microorganisms from various substrates that contain lots of carbon sources including fatty acids and sugars. One of the substrates that contain a lot of carbon sources is waste from palm oil processing plants. PHA-producing bacteria can take advantage of excess carbon sources in palm oil waste in the form of fatty acids to be converted into PHA. This study aims to determine the potential of bacteria isolated from palm oil mill waste to produce polyhydroxyalkanoates (PHA) and to determine the optimum time required for bacteria to produce PHA. Optimization of the fermentation time was carried out at 24, 48, and 72 hours using minimum Ramsay media added with 1% palm oil and 1% glucose. The results of the study obtained 20 isolates of bacteria isolated from palm oil mill waste, and 9 of them were able to produce polyhydroxyalkanoate (PHA) qualitatively. The results of quantitative selection obtained 2 bacterial isolates capable of producing PHA, namely isolates CPS 3 and LPS2 CPS 3, with crotonic acid absorbance values of 41.6 and 5.01, respectively with a fermentation time of 72 hours. Based on the results of genotypic identification using 16S rRNA DNA sequences, isolates of CPS 3, including Bacillus sp. strain CL33 and isolate LPS 2, belonged to Bacillus flexus strain S5a. This shows that isolates from palm oil mill waste can be used as a source of isolate for PHA production.Abstrak: Polyhydroxyalkanoate (PHA) merupakan polimer penyusun plastik biodegredable yang dapat di sintesis oleh mikroorganisme dari bermacam-macam substrat yang banyak mengandung sumber karbon asam lemak dan gula. Salah satu subtrat yang banyak mengandung sumber karbon adalah limbah yang berasal dari pabrik pengolahan kelapa sawit. Bakteri penghasil PHA dapat memanfaatkan sumber karbon berlebih dalam limbah kelapa sawit berupa asam-asam lemak untuk diubah menjadi PHA. Penelitian ini bertujuan untuk mengetahui potensi bakteri yang diisolasi dari limbah pabrik kelapa sawit dalam menghasilkan Polyhydroxyalkanoate (PHA) serta mengetahui waktu optimum yang dibutuhkan oleh bakteri dalam menghasilkan PHA. Optimasi waktu fermentasi dilakukan pada 24, 48 dan 72 jam menggunakan media minimal Ramsay yang ditambahkan minyak sawit 1% dan glukosa 1%. Hasil penelitian memperoleh 20 isolat bakteri yang diisolasi dari limbah pabrik kelapa sawit dan 9 isolat diantaranya mampu menghasilkan Polyhydroxyalkanoate (PHA) secara kualitatif. Hasil seleksi secara kuantitatif diperoleh 2 isolat yang mampu menghasilkan PHA yaitu isolat CPS 3 dengan nilai absorbansi asam krotonoat sebesar 41,6 sedangkan LPS 2 memiliki nilai absorbansi asam krotonoat sebesar 5,01 dengan waktu fermentasi selama 72 jam. Berdasarkan hasil identifikasi secara genotipik dengan menggunakan sekuens DNA 16S rRNA isolat CPS 3 termasuk jenis Bacillus sp. strain CL33 dan isolat LPS 2 termasuk jenis Bacillus flexus strain S5a. Hal ini menunjukkan bahwa isolat dari limbah pabrik kelapa sawit dapat digunakan sebagai sumber isolat untuk produksi PHA.
OPTIMALISASI PENYERAPAN LOGAM BERAT TEMBAGA (Cu) OLEH Alcaligenes faecalis SEBAGAI UPAYA BIOREMEDIASI Ulfa Triyani A Latif; Altriana Eka Putri; Mashuri Masri
CELEBES BIODIVERSITAS : Jurnal Sains dan Pendidikan Biologi Vol 6, No 1 (2023): Science Biology, Education, Conservation, Ecology
Publisher : Universitas Patompo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51336/cb.v6i1.392

Abstract

Penelitian ini bertujuan untuk melihat kemampuan bioakumulasi dari Alcaligenes faecalis terhadap logam berat tembaga (Cu) dengan melihat jumlah penurunan logam berat. Media NA dicampur dengan logam berat CuNO3 sebanyak 10 ppm, diinokulasikan bakteri Alcaligenes faecalis dan diinkubasi selama 24 jam. Kultur bakteri diinokulasikan masing-masing pada media NB 250 ml kemudian di inkubator shaker selama 12 jam dan dilakukan 6 kali pengamatan, diukur suhu dan pHnya. Dilakukan uji UV VVS untuk melihat absorbansinya. Hasil pengamatan disentrifugasi dan supernatan di uji SSA untuk melihat penurunan logam beratnya. Dari hasil penelitian diperoleh bahwa bakteri Alcaligenes faecalis mampu menurunkan kadar logam berat masing-masing dari 10 ppm menjadi 2,60 ppm. Berdasarkan hasil yang diperoleh menunjukkan bahwa bakteri Alcaligenes faecalis dapat dijadikan sebagai agen bioremediasi logam berat tembaga (Cu)
Inhibitory Power of Jatropha Curcas Leaf Extract on the Growth of Escherichia coli, Staphylococcus aureus, and Candida albicans Fungi Sarah Shakina; Yusminah Hala; Mashuri Masri
Contributions of Central Research Institute for Agriculture Vol. 16 No. 4 (2022): October: Agriculture
Publisher : Central Research Institute For Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.269 KB)

Abstract

This study aims to determine the effect of Jatropha curcas leaf extract on the inhibition of Escherichia coli, Staphylococcus aureus, and Candida albicans. This study was an experimental study which was arranged using a completely randomized design (CRD) consisting of 5 treatments (A0 = control (without Jatropha curcas leaf extract), A1 = 5% concentration (5 grams of Jatropha curcas leaf extract/95 ml aquadest), A2 = 10% concentration (10 grams of Jatropha leaf extract/90 ml of aquadest), A3 = 20% concentration (20 grams of Jatropha leaf extract/80 ml of aquadest), A4 = 30% concentration (30 grams of Jatropha leaf extract /70 ml of distilled water) With 3 replications with an incubation period of 24 hours and 48 hours.The parameters observed in this study were the number of clear zones (inhibitory zones) in Escherichia coli, Staphylococcus aureus, and Candida albicans, using a sliding bar. The results showed that the inhibition of Jatropha leaf extract was statistically very significant with a BNT value of a 0.01 = 0.15 and 0.13 and optimum inhibition at a concentration of 20% for Escherichia coli, for Staphylococcus aureus a BNT value of a 0.01 = 0.39 and 0.33 for optimum inhibition at a concentration of 20%, BNT a value of 0.01 = 0.52 and 0.29 for Candida albicans and optimum inhibition at a concentration of 30%.
Co-Authors A, Suci Ananda A. Mustika Abidin Abdul Chadjib Halik Ahmad Ali Ahyar Ahmad Altriana Eka Putri Andiani Andiani Andiani Arfan Arifuddin Arifuddin Arifuddin As Awalia Amir Asbar Hamzah Asep Supriadin, Asep Asfiana Asfiana Asmuddin Natsir Asnilawati Asnilawati Astati Ateng Supriyatna AYU LESTARI Basir Paly Cecep Hidayat Cut Muthiadin Delima Engga Maretha Delima Engga Maretha Dirhamzah, Dirhamzah Donny Suherman Dwi Fitri Yani Ekafadly Jusuf Elmika Nesti Fatmawati Nur Fifi Dismayanti Indriani Nainu Hafsah Hafsah Hafsan Hajrah Hajrah Handayani Handayani Hardiyanti Hamrullah Hartati H, Hartati Hartati Hartati Heriadi Heriadi Ibrahim Ibrahim Ilham, Alya Salsabila Indah Islamiah Inna Shintia Irhamniah Irhamniah Irwan Irwan Isna Rasdianah Aziz Joko Widodo Khaerul Anwar Kurahman, Opik Taupik Laily Agustina, Laily Latif, Ulfa Triyani A. Lianah Lianah, Lianah Lili Silvana Lusi Haerunnisa Maghfirah M Marcia Bunga Pabendon Maretha, Delima Engga Masita Masita Maslan, Muhammad Masriany, Masriany Muhammad Arsan Jamili Muhammad Basri Muhammad Khalifah Mustami Muhammad Nur Hidayat Muhammad, Rusny Murtiadi Awaluddin Nur Haedar Nurhidayah N NURHIDAYAH NURHIDAYAH Nurhidayani Nurhidayani Nurlailah Mappanganro Nurul Afriani Arif NURZAKIYAH NURZAKIYAH Ramlah Ramlah Ramlah Rodiyati Azrianingsih Rosmah, Rosmah Rukmana, Rusmadi Rusny Rusny Rusny Rusny Sappewali Sappewali Sarah Shakina Silvana, Lili Sinar Wahyuni Siska Tri Desianti Siska Tridesianti Siti Patima Sitti Rahmah Sari Sjamsiah, Sjamsiah SRI UTAMI PUTRI Sukmawaty, Eka Suriani Suriani Suriani Suriani Susanti, Handayani Indah Syafaat, M. Aidin Syafrina sari Lubis Syahdar Baba Syahriani Syahriani, Syahriani Tang Silmy Zahrah Tri Cahyanto Tridesianti, Siska Ulfa Nur Alfriani Amir Ulfa Triyani A Latif Uswatul Hasanah Uswatul Hasanah, Uswatul Wahyuni Wahyuni Warobi Warobi Yani Suryani Yani, Dwi Fitri Yuliawati, Astri Yusminah Hala Zasarwati Dwyana