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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Teknologi Industri Pertanian Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Akuatika Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Jurnal Hortikultura Current Biochemistry BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Indonesian Journal of Biotechnology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Sumberdaya Hayati Journal of Tropical Biodiversity and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Purifikasi Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Menara Perkebunan Jurnal Mangifera Edu Journal of Microbial Systematics and Biotechnology Konservasi Hayati Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) MADURANCH: JURNAL ILMU PETERNAKAN Makara Journal of Science International Journal of Oil Palm
NISA RACHMANIA MUBARIK
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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ISOLASI BAKTERI SELULOLITIK DAN KARAKTERISASI ENZIMNYA Meryandini, Anja; Widosari, Wahyu; Maranatha, Besty; Sunarti, Titi Candra; Rachmania, Nisa; Satria, Hasrul
Makara Journal of Science Vol. 13, No. 1
Publisher : UI Scholars Hub

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Abstract

Isolation of Cellulolytic Bacteria and Characterization of the Enzyme. Four of cellulolitic bacteria isolates had been characterized. The determination of cellulase activity was conducted at the highest production time, using crude enzymes with the modification of Miller methods (1959) on pure cellulose substrates such as CMC (Carboxymethyl cellulose), Avicel and Filter paper Whatman No. 1 as well as agriculture waste such as rice straw, corn cob and banana peel. Cellulase from C4-4, C5-1, C5-3 and C11-1 showed optimum activity at pH 5, 70°C, pH 3.5, 90°C, pH 5, 80°C and pH 8, 70°C, respectively. Avicel is a appropriate substrate for C4-4 cellulase whereas CMC for the other three. C11-1 cellulase has the highest cellulase enzyme activity on rice straw substrate whereas C4-4 cellulase on banana peel substrates. C5-1 and C5-3 cellulase have relatively low cellulase activities in degrading substrates of agriculture waste. However, isolates of C5-1 and C5-3 have high cellulase activities on banana peel substrates.
The Potential of Endophytic Bacteria as the Biocontrol Agents of Fusarium proliferatum Ernia, Rima; Mubarik, Nisa Rachmania; Sudirman, Lisdar Idwan
Makara Journal of Science Vol. 27, No. 4
Publisher : UI Scholars Hub

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Abstract

Fusarium proliferatum decreases the productivity of shallot (Allium cepa L.). Endophytic bacteria are potential biocontrol agents in controlling the growth of F. proliferatum. This study aimed to select and characterize endophytic bacteria producing bioactive compounds that inhibit the growth of F. proliferatum. The endophytic bacteria used were isolated from the roots, stems, leaves, and soil of shallot plants from healthy and diseased shallot farming fields. The endophytic bacteria were selected using the dual culture method and extracted using ethyl acetate solvent. The results of the 16S rRNA identification suggested that isolate ABP5.2 is similar to Pseudomonas aeruginosa, while isolates BBP5.2 and DBP4.1 are similar to Myroides profundi. Isolate BBP5.2 was found to have the highest ability (68.15%) to inhibit the growth of F. proliferatum by using its antifungal compounds through a direct mechanism. In addition, the results of the Gas Chromatography-Mass Spectrophotometry analysis suggested that the compounds produced by the crude extracts of BBP5.2 isolate are hexadecanoic acid, 9-octadecenoic acid, linoleic acid, and piperine.
The Potential of Clove Rhizospheric Bacteria to Produce Vanillin from Eugenol Nurfahmi, Riziq Ilham; Mubarik, Nisa Rachmania; Rusmana, Iman; Tambunan, Ika Roostika; Akhdiya, Alina
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.702-711

Abstract

Vanillin is one of the most important flavoring agents worldwide. Currently, consumers' awareness and concern for biovanillin production has been increasing. This study aimed to screen the potential of clove rhizospheric bacteria isolates producing vanillin through a biotransformation process of eugenol and to conduct the preliminary optimization of the biotransformation condition. Twenty-eight bacteria isolates were screened for their capability to transform eugenol into vanillin. BKL 15 isolate, which was identified as Lysinibacillus xylanilyticus, was selected as the highest vanillin producer among the isolates. The optimum molar yield of vanillin produced by the selected isolate was 4.99% (1.11 g/L) after 168 hours of biotransformation process in the medium consisting of TSB (30 g/L), eugenol (24 g/L), yeast extract (20 g/L), and concentration of casamino acid (20 g/L). Throughout the publications we have read, this is the first report of L. xylanolyticus that produces vanillin.
Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp. Maherani, Vincentia Fenice Angger; Mubarik, Nisa Rachmania; Priyanto, Jepri Agung; Putra, Ivan Permana
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.725-732

Abstract

Biosurfactants are a class of amphipathic molecules that various microorganisms can produce. Biosurfactants are used as biopesticides and biocontrol agents because they have antimicrobial activity, especially as antifungal agents in several species of fungal pathogens such as Ganoderma boninense and Fusarium sp. that attack crops. This study aims to detect the biosurfactant activity of Bacillus sp. and its potential as an antifungal agent against the fungi Ganoderma boninense and Fusarium sp. Biosurfactants were produced in mineral salt medium (MSM) by harvesting cell-free supernatants. Screening of biosurfactant-producing isolates was carried out using an oil-spreading assay, a hemolysis assay, and an emulsification index. The antifungal activity of the isolates was then tested using the agar diffusion method. The LP04 isolate was closely related to Bacillus thuringiensis with a 99% similarity level. It has the potential to have biosurfactant activity, which is characterized by a positive result on the oil spreading assay test and has an emulsification index of 48.33±2.87%. The cell-free supernatants of the bacterial isolate were able to inhibit the growth of Ganoderma boninense and Fusarium sp. with growth inhibition rates of 51.11% and 56.92%, respectively.
Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro Wibowo, Risky Hadi; Mubarik, Nisa Rachmania; Rusmana, Iman; Thenawidjaya, Maggy
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.406 KB) | DOI: 10.14692/jfi.13.3.105

Abstract

Chitinolytic bacteria have been reported as biocontrol agents and have the ability to produce chitinase enzymes. The objective of the research was to obtain chitinase producing bacteria that had antagonistic activity to Ganoderma boninense, a causal agent of basal stem rot on oil palm. A total of 63 isolates of chitinase producing bacteria were isolated from soil of Bukit Dua Belas National Park and oil palm plantation in Jambi Province; all was screened for their potency in inhibiting G. boninense in vitro. Three isolates designated TB04-05, SW01-11, and SW02-08 were potentially suppressed and inhibited the mycelium growth of G. boninense in vitro. Based on their specific chitinase activity, these three isolates produced the highest level of chitinase enzyme of 6.3072 U mg-1 protein, 6.0385 U mg-1 protein and 6.1279 U mg-1 protein, respectively after 24 hr incubation. Based on 16S RNA identification, strain TB04-05 had similarity with Bacillus cereus, whereas strains SW01 and SW02-08 had similarity with Bacillus thuringiensis.
Interaksi Molekuler Senyawa Kuersetin dan Eugenol terhadap Protein Regulator Lintasan Penuaan SIR2, pada Khamir Saccharomyces cerevisiae Alfaridza, Annisa Nourma; Astuti, Rika Indri; Mubarik, Nisa Rachmania
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.6819

Abstract

Proses penuaan dapat dipengaruhi oleh beberapa faktor, seperti peningkatan cekaman oksidatif dan inflamasi di mana senyawa antioksidan seperti kuersetin dan eugenol dapat mengatasi hal tersebut. Kuersetin dan eugenol merupakan senyawa antioksidan yang dapat ditemukan di alam. Kuersetin dan eugenol memiliki aktivitas antioksidan pada sel khamir maupun sel mamalia. Namun, mekanisme kedua senyawa ini dalam meregulasi penuaan di level molekuler belum tersedia. Oleh karena itu, penelitian ini dilakukan dengan tujuan untuk mengetahui interaksi senyawa kuersetin dan eugenol pada protein Sir2 pada Saccharomyces cerevisiae yang memiliki peran sebagai regulator sistem penuaan. Penelitian menggunakan studi penambatan molekular dengan senyawa kuersetin dan eugenol sebagai ligan uji, sedangkan reseptornya menggunakan Sir2 asal Saccharomyces cerevisiae dengan kode PDB, 2HJH. Hasil penelitian menunjukkan bahwa senyawa kuersetin dan eugenol dapat berikatan secara molekuler dengan protein Sir2. Parameter afinitas ikatan (ΔG) dilihat dengan nilai negatif yang tinggi. Senyawa kuersetin memiliki afinitas ikatan (ΔG) sebesar -8.5 kkal/mol, sedangkan pada senyawa eugenol memiliki afinitas ikatan (ΔG) sebesar -6.5 kkal/mol. Selain itu, adanya ikatan kimia dan residu asam amino menunjukkan bahwa senyawa ligan uji memiliki potensi untuk bersaing dengan ligan alami. Namun, penelitian lebih lanjut diperlukan untuk menentukan efek ligan uji terhadap lintasan penuaan, in vivo.
Seleksi dan Pengujian Bakteri Biokontrol terhadap Cendawan Patogen Fusarium sp. yang Diisolasi dari Buah Tomat (Solanum Lycopersicum L.): Selection and Testing of Biocontrol Bacteria against Pathogenic Fungi Fusarium sp. Isolated from Tomato Fruits (Solanum lycopersicum L.) Ayni, Qurrotu; Sudirman, Lisdar I; Mubarik, Nisa Rachmania
Jurnal Sumberdaya Hayati Vol. 11 No. 1 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.1.23-30

Abstract

Fusarium oxysporum is one of the primary pathogens in tomato plants that causes plant wilting. Pathogenic fungi have been controlled by spraying synthetic fungicides. The negative impact of excessive use of synthetic chemicals can be reduced by using biocontrol agents that produce antifungal compounds. Natural pest control efforts use endophytic bacteria and rhizosphere as biocontrol agents for fungal pathogens on tomato plants (Solanum lycopersicum L). This study aims to select and test biocontrol bacteria against pathogenic fungi Fusarium sp. isolated from tomato fruits. Fusarium successfully isolated from tomato fruits which was characterized based on observations of colony and cell morphology. A total of 15 rhizosphere bacterial isolates were tested against Fusarium sp., and 11 bacterial isolates were able to inhibit the growth of the fungus. Isolates TTSG 2.2, TTSG 2.7, TTSG 3.2, TTSG 3.5, TTSG 3.6, and TCS 3.1 had fungal inhibition of 45%. The results of the hemolysin test showed that one isolate, TTSG 3.6 isolate, was negative, which did not form a clear zone around the bacterial streak. Isolate TTSG 3.6 still showed a few brown spots and rotted tomato fruit, so this bacteria needs to be tested for hypersensitivity on tobacco leaves to show its pathogenicity in plants.
Increasing Hydrolytic Activity of Lipase on Palm Oil by PCR-Based Random Mutagenesis Maria Dita Febriani Lumban Gaol; Andreas Adhi Satya; Esti Puspitasari; Nisa Rachmania Mubarik; Antonius Suwanto
International Journal of Oil Palm Vol. 3 No. 3 (2020): September 2020
Publisher : Indonesian Oil Palm Society /IOPS (Masyarakat Perkelapa-sawitan Indonesia /MAKSI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35876/ijop.v3i3.53

Abstract

ABSTRACT Random mutagenesis technique is a powerful technique capable of producing enzymes with desired biocatalytic activity. This study aims to obtain a mutant lipase with improved hydrolytic activity on palm oil substrate using random mutagenesis technique. Random mutagenesis by error-prone PCR was used to generate mutant lipases. A total of 1101 mutants were obtained, out of which two mutants, Lip M14.25, and Lip M14.57, showed an increased relative hydrolytic activity. Lip M14.25 and Lip M14.57 demonstrated a 14% and 16% increased activity respectively. A comparison of the mutants' hydrolytic activities using p-nitrophenyl esters showed a significantly high preference for p-nitrophenyl palmitate. Furthermore, the mutant, Lip M14.25 showed its highest activity at pH 5, and Lip M14.57 exhibited a 10 oC decrease in optimum temperature. The two mutants' protein modelling showed the substitution of N44S/S202N on M14.25 and F154L/S265C on M14.57 lipase, which caused changes in conformation and active site residue distance of the lipase. The study found two mutants of lipase, M14.25 and M14.57, which showed improved hydrolytic activity on palm oil substrate.
Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS Nuraliah Rusman; Prayoga Suryadarma; Nisa Rachmania Mubarik
AGROINTEK Vol 19, No 1 (2025)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v19i1.11110

Abstract

Microorganisms as cell factories in producing a product are strongly influenced by their metabolism. engineering microbial metabolism by increasing coenzymes that play a role in enzyme activity. Thiamine diphosphate is one of the coenzymes that are commonly found in the metabolic pathways of microbial cells. Increasing the thiamine formation pathway as a precursor in the thiamine metabolic pathway is a strategy in this study. Enhancing thiamine accumulation in Escherichia coli culture by inducing oxygenation level and deleting rpoS was investigated. The investigation was conducted by comparing the cultivation of  Escherichia coli BW25113 (parent strain) and BW25113-∆rpoS (rpoS mutant) cultures under aerobic conditions at different shaking rotation rates of 150 and 200 rpm. The measured parameters were thiamine accumulation, dry cell weight, acetate concentration and glucose consumption of the cultures. Inducing oxygenation level of parent strain cultures through increasing shaking rotation rate from 150 to 200 rpm induced 40 % thiamine accumulation. Meanwhile, deletion of rpoS increased 9,5 % thiamine accumulation compared to parent strain culture
Optimasi Proses Biodrying: Penggunaan Formulasi Konsorsium Mikroorganisme dalam Bahan Pembawa Zaenal Arifin, Sukarya; Rachmania Mubarik, Nisa; Rusmana, Iman
Konservasi Hayati Vol 21 No 1 (2025): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v21i1.37665

Abstract

Household organic waste generated daily holds potential for conversion into bioenergy through biodrying technology. Biodrying is a biological drying process that relies on microbial activity to reduce the moisture content in organic waste, producing solid fuel. This study aimed to formulate a microbial consortium for biodrying applications and to compare the effectiveness of solid (talc-based) and liquid (molasses-based) carriers on biodrying performance and inoculant stability. The consortium comprised Lysinibacillus halotolerans, Bacillus soronensis, Lactobacillus rhamnosus, Pichia manshurica, and phosphate-solubilizing bacteria. Isolate rejuvenation was performed using selective culturing followed by characterization. Microbial formulations were developed in both solid and liquid forms and applied to organic waste. Key parameters observed included moisture content, temperature, pH, and inoculant viability over a three-month storage period. The results showed that both talc and molasses carriers were more effective than commercial controls, as indicated by higher temperatures (44.1°C and 43.66°C), lower moisture content (44.5% and 45.25%), and favorable pH values (5.8 and 6.35), which supported mesophilic and thermophilic microbial activity during the biodrying process. Peak microbial activity occurred on day four, predominantly driven by Bacillus species during the thermophilic phase. Inoculant stability was also higher in the talc-based formulation, potentially due to its magnesium and silicon content, which contributed to maintaining microbial viability during storage.
Co-Authors , Triadiati . QATRUNNADA Aas Ratnasari Ahmad Suryadi Ainia Hanifitri Alfan Cahyadi Alfaridza, Annisa Nourma ALINA AKHDIYA Andreas Adhi Satya Andreas Adhi Satya ANGELIA REZTY FITRIANI SITUMORANG Anggreandari, Rizky Ani Suryani Ani Suryani Anja Meryandini Antonius Suwanto Aris Tjahjoleksono Aris Tri Wahyudi Atang Sutandi Ayni, Qurrotu Bambang - Riyanto Besty Maranatha BRAMANTYO JATI PRASOJO Budiasih Wahyuntari Budiasih Wahyuntari Budiasih Wahyuntari Cahyadi, Alfan Dadang Suhendar Dadang Suhendar Delfi Trisnawati DERI YURATMOKO Desniar - - Dewi Seswita Zilda Dewi Seswita Zilda Dian Syahfitri Dinamella Wahjuningrum Dwi Ambarawati DWI ANDREAS SANTOSA Ekowati Chasanah Encah Ewi Mulyeti Esti Puspitasari ESTI PUSPITASARI Evi Damayanti Evi Damayanti, Evi Fauzy Rachman Fauzy Rachman Fauzy Rachman Ferymon Mahulette Ferymon Mahulette Ferymon Mahulette, Ferymon Fitratul Aini Fitratul Aini, Fitratul Fitriani Idham Galih Cendana Nabilasani Galih Cendana Nabilasani Hadi Susilo Hamim Hamim Hana Nurullita Prestisia Hasrul Satria Hasrul Satria, Hasrul HENDRA PARDEDE Hirmas Fuady Putra HIRMAS FUADY PUTRA, HIRMAS FUADY Idin Abidin Ika Roostika Tambunan, Ika Roostika Ika Setianingsih, Ika Iman Rusmana Iman, Rusmana Ismi Isti'anah Isna Rahma Dini Iswati, Ruma It Jamilah Ivan Permana Putra Jepri Agung Priyanto Jepri Agung Priyanto, Jepri Agung Karina Eku Dwinanda Gunawan Kusuma Handayani LAKSMI AMBARSARI Lia Siti Nur'amaliyah Lia Siti Nur'amaliyah Lisdar Idwan Sudirman Listyowati, Sri lmiah, Sitti Nur Luky Adrianto Maggy Thenawidjaya Suhartono Maherani, Vincentia Fenice Angger Maranatha, Besty Maria Dita Febriani Lumban Gaol Marini Adani Mashudi Mashudi Maslahah, Iah Novi Masrukhin Masrukhin Muhamad Azwar Syah Mulyorini Rahayuningsih Mutiha Panjaitan Nabilasani, Galih Cendana Nabilasani, Galih Cendana Ninda Ningtyas Nuraliah Rusman Nurfahmi, Riziq Ilham Nurul Hidayati Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Prayoga SURYADARMA Puspita Lisdiyanti Rika Indri Astuti RIMA ERNIA Risky Hadi Wibowo Risky Hadi wibowo Rizky Anggreandari Rury Eryna Putri Sarah Asih Faulina Sarah Asih Faulina, Sarah Asih Sipriyadi Sipriyadi Sipriyadi Siswa Setyahadi Siswodarsono, Trismilah Siswodarsono, Trismilah Siti Azzira Rahma Sitti Nur Ilmiah Sonya Tobing Sri Budiarti Poerwanto Sri Listyowati Suhendar, Dadang Suhendar, Dadang SYAMSUL BAHRI SYAMSUL BAHRI TEDJA IMAS Thenawidjaya, Maggy Titi Candra Sunarti TRI ASMIRA DAMAYANTI Tri Handayani Kurniati Tri Handayani Kurniati Trismilah Trismilah Siswodarsono Untung Sudadi Wahyu Widosari Wibowo, Risky Hadi WIDANARNI WIDANARNI Widanarni Widanarni Widosari, Wahyu Yoan Ramasita Yusro Nuri Fawzya Zaenal Arifin, Sukarya Zulfarina Zulfarina Zulfarina,