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Uji Fitokimia Ekstrak Daun Sukun Kering (Artocarpus altilis) Endang Tri Wahyuni Maharani; Ana Hidayati Mukaromah; Meka Zainal Farabi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2014: PROSIDING SEMINAR NASIONAL HASIL - HASIL PENELITIAN & PENGABDIAN
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Daun sukun (Artocarpus altilis) adalah salah satu obat tradisional yang telah banyak dikenal masyarakat Indonesia. Flavonoid, artoindonesianin, asam hidrosianat, asetilcolin, tannin,riboflavin, saponin, phenol, quercetin, champerol dan kalium merupakan kandungan kimia daunsukun yang berkhasiat sebagai pengobatan. Daun sukun berkhasiat mengobati berbagai penyakit seperti ginjal, jantung, tekanan darah tinggi, liver, pembesaran limpa, kencing manis, asma, dan kanker. Hampir seluruh bagian tanaman sukun (Artocarpus altilis) mulai dari akar, bunga, daun,buah, batang, dan getahnyapun dapat dimanfaatkan untuk keperluan hidup manusia dan berkhasiat mengatasi berbagai gangguan kesehatan.Tujuan jangka panjang penelitian ini adalah mengkaji pemanfaatan daun sukun (Artocarpus altilis) sebagai obat penyakit batu ginjal dan menganalisis zat aktif (alkaloid, flavonoid, sterol,triterpenoid, glikosida steroid, tannin, fenolik dan saponin) berkhasiat pada daun sukun(Artocarpus altilis) Sampel penelitian adalah daun tanaman sukun yang diambil dari desa Kronggen kabupaten Grobogan kota Purwodadi, sebanyak 2 kilogram, digunakan 10 lembar sebagai sampel penelitian. Daun sukun dipilih yang berwarna hijau tua, dicuci bersih dan dibuang tulang daunnya, kemudian dikeringkan di bawah terik matahari selama 5 hari dan diserbuk. UjiFitokimia: Ekstraksi dilakukan dengan teknik refluks menggunakan eter, kemudian residunya diekstraksi menggunakan metanol p.a., selanjutnya diekstraksi menggunakan metanol 50% dan hasil ekstraksi kemudian diuji penapisan fitokimia meliputi Alkaloid, flavonoid, fenol, sterol,tannin dan saponin. Hasil uji fitokimia ekstrak methanol daun sukun kering (Artocarpus altilis)  mengandungsalkaloid, flavonoid, fenol, saponin dan tannin.Kata kunci : Uji Fitokimia, Ekstrak Methanol, Daun Sukun Kering (Artocarpus altilis)
PROFIL PROTEIN DAGING IKAN BANDENG (Chanos chanos) MENGGUNAKAN SDS-PAGE SEBELUM DAN SESUDAH PENGGARAMAN Feri Feri; Stalis Norma Ethica; Ana Hidayati Mukaromah
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Milkfish (Chanos chanos) is potential source of easily digested, yet easily decayed animal protein. Salting using table salt (NaCl) is a common technique used to prevent early spoilage on milkfish meat. In study, the effect of salting on of milkfish was investigated using SDS-PAGE method. The aim were: 1. To evaluate protein profile before and after salting in milkfish at varied salt concentration and salting time. 2. To recommend milkfish salting process based on denaturation level of protein reflected by changes in protein profile compared to that of control. Seven portionsof meat from one fresh milkfish was used as samples (6 portions) and control (1 portion). All samples were salted using NaCl at concentration of 10, 20, and 30% b/b in varied salting time of 30 and 60 mins. The results showed that the milkfish meat before salting process (control) had atotal 15 protein bands. The total protein band number decreased in samples salted for 30 mins at NaCl concentrations of 10, 20 and 30% b/v to become 14, 14 and 12 bands respectively. Further decrease of the band number was observed in samples salted for 60 mins at NaCl concentrations of 10, 20 and 30% b/v where the number became 12, 11 and 10, respectively. Molecular weightanalysis on these results showed that salting process of milkfish for 30 min at NaCl concentration of 10% b/v is most recommended as its profile protein showed the least change of protein bands from control’s.Keywors: Penggaraman, Ikan bandeng profil protein, SDS-PAGE
PROSES HIDROLISIS ONGGOK DENGAN VARIASI ASAM PADA PEMBUATAN ETHANOL - Yusrin; Ana Hidayati Mukaromah
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2010: PROSIDING SEMINAR NASIONAL HASIL-HASIL PENELITIAN
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Latar Belakang :Onggok adalah serat yang merupakan hasil samping pembuatan pati dari ubi kayu(cassava). Serat onggok terdiri dari hemiselulosa, pektin dan selulosa, serta hasil sementara menunjukkanbahwa penambahan asam 20 ml merupakan kondisi optimal untuk proses hidrolisa pati dari onggok dankurang lebih 80% onggok mampu terhidrolisa menjadi glukosa pada 24 jam fermentasi (TrisantiAnindyawati, 2007). Obyek Penelitian : onggok yang diambil dari daerah Pati. Sampel yang diambildihidrolisis dengan HCl 1% - 5%, H2SO4 1%-5%, H2C2O4 1% - 5%, kemudian hasil hidrolisisdifermentasi dengan ragi hasil optimasi, waktu fermentasi hasil optimasi. Hasil fermentasi didestilasi dandilakukan penetapan kadar alkohol. Hasil penelitian : Otimasi konsentrasi asam, penambahan jumlah ragi,waktu fermentasi untuk menghidrolisis onggok yang dapat menghasilkan kadar ethanol maksimumadalah asam 3%, jumlah ragi 1%, dan waktu fermentasi 32 jam. Kadar glukosa pada hasil fermentasionggok yang dihidrolisa dengan asam klorida, asam sulfat, dan asam oksalat dengan konsentrasi asam,jumlah ragi, dan waktu fermentasi hasil optimasi berturut-turut adalah 23,73%, 23,88% dan 20,43%.Kadar ethanol pada hasil fermentasi onggok yang dihidrolisa dengan konsentrasi asam, jumlah ragi, danwaktu fermentasi hasil optimasi adalah untuk asam klorida 8,94% b/b, asam sulfat 9,11%, dan asamoksalat 6,93% b/b. Jenis asam untuk menghidrolisis onggok yang menghasilkan kadar ethanol palingmaksimal adalah asam klorida dan asam sulfat.
PENURUNAN KADAR Fe DALAM AIR DENGAN BIJI KELOR (Moringa oleifera) Yusrin -; Ana Hidayati Mukaromah; Endang Tri Wahyuni M
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Latar Belakang : Tanaman Moringa oleifera banyak tumbuh di India bagian utara, tetapi sekarang sudah menyebar luas ke seluruh kawasan tropis, termasuk Indonesia.Penelitian tentang biji kelor sebagai koagulan pada kekeruhan dan penurunan kadar unsur logam berat (Fe, Mn, Cu, Cr) dalam air telah dilakukan sebelumnya. Hasil penelitian Enos Tangke Arung, MP, dosen dari Fakultas Kehutanan (Fahutan) Universitas Mulawarman (Samarinda) menemukan biji kelor yang diadopsi dari Negara Sudan, dan menyulapnya menjadi ''serbuk ajaib'' yang dapat mengubah air keruh dengan partikel tanah maupun unsur logam menjadi air bersih layak konsumsi, dan memenuhi standar baku mutu yang ditetapkan. Banyak orang yang belum mengetahui bahwa biji kelor dapat dijadikan alternatif penjernih air yang lebih aman selain tawas. Biji kelor juga lebih ekonomis dibandingkan tawas. Obyek Penelitian : Pengambilan biji kelor diperoleh dari Balai Benih Induk Palawija, Kabupaten Lawang, Malang, Jawa Timur. Sedangkan air yang digunakan adalah air yang mengandung Fe 10 ppm. Hasil penelitian : Nilai rata-rata prosentase degradasi yang dihasilkan yaitu dengan waktu perendaman dari 0-15 menit sebanyak 38,96%, dari 15-30 menit sebanyak 43,28% (kenaikannya sebesar 4,32% ), dari 30-45 menit sebanyak 45,35% (kenaikannya sebesar 2,07%) dan dari 45-60 menit sebanyak 48,16% (kenaikannya sebesar 2,81%). Namun demikian waktu yang paling efektif untuk proses degradasi ion Fe(II) yaitu 30 menit dengan rata-rata hasil degradasi sebanyak 43,28% dengan kenaikan ion Fe terdegradasi sebesar 4,32% dari hasil rata-rata dengan perendaman selama 15 menit. Semakin lama waktu perendaman dengan penambahan 6 biji kelor terhadap larutan uji Fe(II) maka semakin bertambah pula jumlah ion Fe(II) yang mengalami degradasi.Kata kunci : Kadar Fe, Biji Kelor, Air, Orthofenantrolin
PROFIL PROTEIN BERBASIS SDS-PAGE ULAT SAGU (RHYNCHOPHORUS FERRUGINESUS) HASIL PEMANGGANGAN DENGAN OVEN DAN MICROWAVE Sri Elvira; Ana Hidayati Mukaromah; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Sago larvae (Rhynchophorus ferruginesus) is a source of animal protein originated from Papua, which has a high protein content. One of the disadvantages of sago larvae as a food ingredient is that it decomposes easily. To avoid decay, preservation could be done by heating with an oven and microwave, but the influence of the heating process to the quality of protein needs to be investigated. The purpose of this study was to analyze the profile of sago larvae protein baked in an oven and microwave with a time variation of sago larvae. The method used was SDS-PAGE (Sodium Dodecyl Sulfate– Polyacrylamide Gel Electrophoresis). The samples used were 13 sago larvae. Alarvae sample was used as a control and was not roasted with an oven and microwave, 6 larvae were baked with an oven with a variation of time 1, 2 and 3 minutes then the other 6 were roasted by microwave with a time variation of 1, 2 and 3 minutes. The results showed that sago larvae as a control had a number of protein bands 26, unlike the protein bands after baking with an oven and microwave. Larvae that have been baked in the oven for 1 minute found 17 protein bands, 20 protein bands were found for 2 minutes, and for 3 minutes were found 10 protein bands. Whereas in the sago larvae sample which was baked in the microwave for 1 minute found 16 protein bands, for 2 minutes found 11 protein bands and for 3 minutes found 12 protein bands. These results indicatedthe longer the heating time, the higher the level of protein denaturation.This marked by more protein bands on protein profile with smaller molecular weight values.Keywords: roasting, sago larvae, SDS-PAGE
ISOLASI DAN IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM PROTEASE PSEUDOMONAS STUTZERI ISTD4 DARI TEMPE GEMBUS PASCA FERMENTASI 1 HARI Wa Ode Inayatul; Sakti Imam Muchlissin; Ana Hidayati Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Proteaseis  a group  of enzymes that  play  an important  role in  biochemical reactions, whichc a use protein break down. Protease is among main enzymes used in industry, which commercial value reach 60% of total enzymes world wide. This study a imed toisolat protease-producing bacterium found on tempe gembus in after 1-day post-fermentation and to identify the bacterial isolat obtained based on the analysis of its 16S rRNA gene. Isolati on and purification process wasd one using Nutrient Agar media with spread technique. The protease production test was carried out on skim milk agar medium. The molecular identification process was performed by analyzing sequence of 16S rRNA gene fragment of bacteria amplified using both forward primer F (F:5'- AGAGTTGATCCTGGCTCAG-3'), and reverse primer R (R:5'- GGTTACCTTGTTACGACTT-3') by Polymerase Chain Reaction (PCR) method. The amplified DNA from PCR was then sequenced. From the isolation process a bacterial strain that has a proteolytic activity based on observation of clear zone area with a diameter of 85 mm was obtained. From sequence alignment result using BLAST (Basic Local Alignment Search Tool) the fragment of 16S rRNA gene of strain ISTD1.4 obtained has similarity level of 98% with fragment of 16S ribosomal RNA gene of bacterium Pseudomonas stutzeri strain E141. In conclusion, strain ISTD1.4 is a potential protease-producing bacteria and is identified as Pseudomonas stutzeri ISTD4. Keywords: Bacterial isolation, molecular identification, proteolytic bacteria, 16S rRNA gene
UTILIZATION OF TIO₂ IMPREGNATED ZEOLIT-ZSM-5 TO DECREASE CONCENTRATION OF CR (VI) IN SOLUTION AT pH VARIANCE Siska Nurprihandayani; Stalis Norma Ethica; Ana Hidayati Mukaromah
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: PROCEEDING 1ST INSELIDEA INTERNATIONAL SEMINAR ON EDUCATION AND DEVELOPMENT OF ASIA (INseIDEA)
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Chromium (VI) or Cr (VI) is one of heavy metal ions, which presence in the environment comes from industrial waste water disposal such as metallic coating, leather tanning and paint industry. Cr (VI) ions are toxic as they could cause lung cancer, chronic infection and polyps. An effort to decrease Cr (VI) concentration has been done by using TiO₂ impregnated Zeolite ZSM-5 (TiO₂-ZSM-5) at 1,25% w/v concentration with pH variation within 75-minute of UV exposure time. This study aims to investigate the effect of pH variation after addition of TiO₂- ZSM-5 powder on Cr (VI) solution, in a way to reduce the presence of the toxic ions in its solution. Object of this study was Cr (VI) solution at concentration of 50 mg/L. The evaluation was carried out on Cr (VI) solutions as samples including vontrol after adding Zeolite ZSM-5 alone, TiO₂ alone and TiO₂ impregnated Zeolite ZSM5 powder. Data analysis was carried out statistically using One-way Annova.The results showed that initial Cr (VI) concentration was 49,73 mg/L and the percentages of Cr (VI) decrease at pH 2, 4, 6, 8 and 10 respectively were 37,55; 34,72; 25.88; 18,10; 11.11%. It means that the highest percentage of Cr (VI) concentration decrease was at pH 2. Based on the statistical analysis results (p value 0,000), the most significant effects in the decreaseof concentation of Cr (VI) solution used in this study were shown by the addition of TiO₂-ZSM-5 powder and by addition of H+ ion (causing pH=2) into the used solution. As conclusion, treatment using TiO₂ impregnatedZeolite ZSM-5 at pH 2 is potential to be used as a way to handle water pollution caused by Cr (VI).
Penggunaan self cleaning Fotokatalis Tio2 dalam Mendegradasi Ammonium (NHd) Berdasarkan lama waktu penyinaran Ana Hidayati Mukaromah; Muh. Amin; Sri Darmawati
JURNAL KESEHATAN Vol 3, No 1 (2010): Ilmu-Ilmu Kesehatan
Publisher : JURNAL KESEHATAN

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Abstract

Ammonium is NH a ' ions thdt are not colored, smelly and dangerous to health, its concentrqtion determined by spectrophotometric method. Ammonium which is atkalini when exposed i tignt or heat will cause odor, because the smell of ammonia' generated, needed a technologt to reiuce or eliiinate the levels of ammonium. Problems of this research is what percentage of degrqdalion of ammonium (NH4 +) with 20 mg of photocatalyst TiO 2 based on the exposure time?The general obiective of this research is to study the degradation of ammonium (NH 4 +) with TitaniumDiol<sida photocatalyst (TiO 4 20 mg based on exposureiime 30, 60: g0, 120, 240, 360, 480, 600, 900, 1500 minutes. Special purpose in this study are: Peiform initial optimization siudy is determine the optimum concentration of ammonium that can produce the mmimum percent ammonium degradation with the number of photocatalyst TiO 2 Titanium Dioxide 20 mg durig the time of 120 minutes. Doing degradation of ammonium with ammonium concentrqtion optimim withlhe number of photocatalyst TiO 2 2b mg for varying exposure time 30, 60, 90, 120, 240, 360, 480, 600, 900, I500 minures.The research object is a solution of ammonium produced in the chemical laboratory of the concentration of 100 ppm was reduced to 10, 20, i0, 40 ppm and then determined the optimui concentration of ammonium. Percent degradation of ammonium with an optimum concentration wiih the addition of titanium dioxide photocatalyst TiO 2 20 mg with varying exposure time 30, 60, 90, t 20, 240, 360, 480, 600,-900, t 500minutes each performed three times repetition.The results showed that the optimum concentration of ommonium NHr*) with photocatalytic TiO2 20 mg over 120 minutes is 30 ppm. Degradation of the ion (NHr') with the variation ofradiarion SO, AO, g0, 120, 240, 360, 180, 600, 900, and 1500 minutes with the optimum concentration of 3i ppm of ammonium and the number of photocatalyst TiO 2 20 mg is five consecutive, 66ok, 6.06%, 6.64%, Z.iZbZ, A.Otm, g.64%, g.5g%,10.52%o, ll.0B%, 11.40%. The longer the exposure time the greater the percent degradation of the ion (NH4 +)Keywords: Degradation of ammonium, Tio2 Photocatalyst, Irradiation time.
EFFECTIVENESS OF SECANG WOOD (Caesalpinia sappan l) CONCENTRATION AS NATURAL INDICATOR FOR ACIDIMETRY METHOD Sri Riris Septianingsih; Ana Hidayati Mukaromah; Endang Tri Wahyuni
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: PROCEEDING 1ST INSELIDEA INTERNATIONAL SEMINAR ON EDUCATION AND DEVELOPMENT OF ASIA (INseIDEA)
Publisher : Universitas Muhammadiyah Semarang

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Abstract

cidimetry is a titration method used to determine acidic levels such as H2SO4,ISBN: 978-602-5614-24-8 Page 245 3 and HCl with methyl red                       (MR)1% w/v as indicator. As synthetic indicator, MR is relatively expensive, therefore a replacement indicator isneeded. This study aimed to investigate the potential of a natural indicator from secang wood (Caesalpiniasappan l) containing brazillin compounds to be used as replacement of MR. The efectiveness of woodconcentration 0.5; 1.0; 1.5 and  2 % w/v used as indicator of acidimetric titration, particularly for standardizingHCl solution was measured. Next, the minimal concentration of wood secang which can be used as indicator ofacidimetric titration was determined. The result showed that secang wood has potential to be used asreplacement indicator of MR. The percentage difference of concentration of HCl solution using MR indicator1.0% with wood concentration 0,5; 1.0; 1.5 and 2.0 % w/v were 6.7; 6.7; 10.0; and 21 % respectively. Minimumconcentration of wood secang that can be used as indicator in acidimetry is 0.5 %b/v.Keywords: acidimetry, secang wood, natural indicator, and synthetic indicator MR
DAYA HAMBAT EKTRAK ETANOL BUAH BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Staphylococcus epidermidis SECARA IN VITRO Asri Rahmiati; Sri Darmawati; Ana Hidayati Mukaromah
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Acne infection is caused inflammation of pilosebasea accompanied by accumulation of keratin material, caused by S. aureus and S. epidermidis bacteria. The community uses of wuluhstarfruit (Averrhoabilimbi L) as a traditional medicine to treat acne infection. Wuluh starfruit contains flavonoids, alkaloids, tannins, and saponins that act as anti microbial. The aim of this study was to analyze the inhibitory power of wuluh starfruit ethanol extract on the growth of S. aureus and S. epidermidis bacteria. The method used in this research is the diffusion of wells. This research used two types of bacteria. S.aureus and S.epidrmidis, each bacteria of the four treatment groups that is 10%w/v; 20%w/v; 30%w/v; 40%w/v; positive control of Ciprofloxacin, and negative control of sterile aquades. The research results of inhibitory power of ethanol extract of wuluh starfruit with variation of concentration 10 %w/v; 20 %w/v; 30 %w/v; and 40 %w/v successively in S.aureus was 21.6 mm; 27.0 mm; 31.3 mm; And 34.0 mm, whereas in S.epidermidis is 28.6 mm; 31.6 mm; 36.3 mm; And 39.0 mm. Then the positive control of Ciprofloxacin has an inhibit zone of 30.0 mm and 35.0 mm. While the negative control of sterile aquades is not formed inhibit zone. The result ofOne Way Anova statistic test on S. aureus is p=0.000 and S. epidermidis is p=0.000, because (p<0.05) hence result there is significant difference, so it can be concluded that the extract of wuluh starfruit ethanol can inhibit the growth of S. aureus and S. epidermidis and there is a significant difference between the variantconcentration ofethanol extract wuluhstarfruit. Keywords: Staphylococcus aureus, Staphylococcus epidermidis, Ethanol extract of wuluhstarfruit.