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All Journal journal of internal medicine Microbiology Indonesia Jurnal NERS Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Chemistry Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) ANIMA Indonesian Psychological Journal Heart Science Journal Indian Journal of Forensic Medicine & Toxicology Narra J Jurnal Natural Folia Medica Indonesiana
Nasronudin Nasronudin
Faculty Of Medicine, Universitas Airlangga, Surabaya. Indonesian-Japan Collaborative Research Center For Emerging And Re-emerging Infectious Diseases, Institute Of Tropical Disease, Universitas Airlangga, Surabaya.
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

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Journal : Jurnal Natural

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HIV Genotype Analysis from HIV Infected Patients in East Java Area Yulia Sari Ismail; Soetjipto Soetjipto; Eddy Bagus Wasito; Nasronudin Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
Co-Authors . PURWATI Achmad Ardianto, Achmad Adiana Mutamsari Witaningrum Adiana Mutamsari, Adiana Agung Prasetiyo Aksono HP., Eduardus Bimo Aldise Mareta Nastri, Aldise Mareta Arfianti Arfianti Arfianto V Arief Suseno, Arief Artaria Tjempakasari, Artaria Asmarawati, Tri Pudy Atika Atika Badi'ah, Hanim Istatik Bambang Prajogo Bimo Aksono Bimo Dwi Lukito, Bimo Dwi Bramanton Bramantono Bramantono, Bramantono Budi Utomo Danny Irawan Djojosugito, Fauzia A. Dwi Wahyu Indriati, Dwi Wahyu Eddy Bagus Wasito Eddy Wasito, Eddy Edith Frederika, Edith Endah Dewati, Endah Endang Retnowati ENDANG RETNOWATI KUSUMOWIDAGDO Endra Gunawan Fedik Abdul Rantam Ferdiansyah Ferdiansyah Ferry Effendi Ganden Supriyanto Hadi U Hamidah - Hening Laswati Putra Indrati, Agnes R. Irine Normalina, Irine Irma Yuliawati, Irma Johana E. Prawitasari Juniastuti Juniastuti Khairunisa, Siti Khairunisa, Siti Q. Lilis Mundri Jannah, Lilis Mundri M. Qushay, M. Maria Inge Lusida Masanori Kameoka, Masanori Maskoep, Wiwiek Indriyani Mei Wulan, Sri Mardjiati Meilani Meilani, Meilani Muhammad Qushai Y. Matondang, Muhammad Qushai Y. Muhammad Qushai Yunifiar M, Muhammad Qushai Muhammad Vitanata Arfijanto, Muhammad Vitanata Musofa Rusli Ni Kadek Mulyantari Ni Nyoman Tri Puspaningsih Nur Syamsiatul Fajar, Nur Syamsiatul Nurlaila Effendy Nuzul Qur'aniati Prihartini Widiyanti Purwaningsih - Purwati - Purwati Purwati Rachman, Brian E. Rachman, Brian Eka Rahayu Anggraini Rahmat Zainuddin, Rahmat Retno Indrawati Roestamadji Retno Pudji Rahayu Ririek Parwitasari, Ririek Rudi Wisaksana Rury Mega, Rury Shuhei Ueda, Shuhei Siregar, Fajri M. Siti Qamariyah Khairunisa, Siti Qamariyah Siti Qomariyah Khairunisa, Siti Qomariyah SOETJIPTO . Soewandojo E Stefanus Supriyanto Suharto - Supriyanto Suryono Suryono Thomas Dicky Hastjarjo, Thomas Dicky Tigor Yeheskiel, Tigor Tjahjono, Ganis Tomohiro Kotaki, Tomohiro Triyono EA Ulfa Kholili Usman Hadi Yayuk Susilawati, Yayuk Yulia Ismail Yulia Sari Ismail