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All Journal El-Hayah : Jurnal Biologi Jurnal Berkala Epidemiologi Indonesian Journal of Tropical and Infectious Disease TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Nusantara Science and Technology Proceedings Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Multidisciplinary Applied Natural Science AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Jurnal Kedokteran Hewan Jurnal Agro Veteriner Folia Medica Indonesiana
Dadik Raharjo
Department Of Veterinary Public Health, Faculty Of Veterinary Medicine, Universitas Airlangga
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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Logo, Label, and Packaging Improvement on Jamu Bu Uri Products for Digital Marketing Kartini; Ratna Yulistiani; Dadik Raharjo; Rizky Fatkhur Rohman; Sri Alam Syah Lihan Candra Asi
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3342

Abstract

It is quite common knowledge that some traditional herbal medicine sellers used plastic bottles of mineral water as a storage medium for their traditional herbal medicine. Even though the allotment of mineral water bottles is dispossible, it cannot be used again after the contents run out. This problem about the packaging must be addressed. When viewed from the visual aspect, the presentation of traditional herbal medicine still tends to be monotonous in terms of packaging. Whereas packaging can influence consumers to give a positive response, good packaging will optimize profits. Therefore, this service activity targets several improvements in handling packaging problems for Jamu bu Uri products, with the aim of increasing the marketing of traditional herbal products.
Effectiveness of the California Mastitis Test (CMT), Reductase Test, and Alcohol Test for Dairy Cows Subclinical Mastitis Detection Eriza Rosalina Rochmah; Dadik Raharjo; Sri Hidanah; Mustofa Helmi Effendi; Adiana Mutamsari Witaningrum; Sunaryo Hadi Warsito
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51443

Abstract

Mastitis is an inflammation of the mammary glands caused by pathogenic bacteria Mastitis divided into 2 types, clinical mastitis which has symptoms of swelling and changes in the shape of the mammary gland, while subclinical mastitis does not indicate clear symptoms. Subclinical mastitis is a very high case in Indonesia, around 97-98%, while clinical mastitis is 2-3%. Effectiveness of the california mastitis test (CMT), reductase test and alcohol test to detect subclinical mastitis in dairy cattle. The number of samples taken was 60 samples of fresh dairy milk from 15 cattles in Medowo Village as much as 50 ml for each sample. The results showed that the test was very effective in identifying subclinical mastitis in dairy cattle. 15% positive results were obtained for subclinical mastitis using CMT, 10% of the milk quality was not good during the reductase test and 18.33% positive with the alcohol test from a total of 60 samples obtained.
Detection of csg and lux Genes in Biofilm-Forming Uropathogenic Escherichia coli Associated with Urinary Tract Infections Rini Purbowati; Sri Lestari Utami; Dadik Raharjo; Masfufatun Masfufatun
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.222

Abstract

Uropathogenic Escherichia coli (UPEC) is responsible for 80–90% of urinary tract infections (UTI) in the global population. The emergence of the increasing resistance to broad-spectrum antimicrobial agents was due to the ability to form biofilms. Cell surface factors that play a role in biofilm formation include Quorum Sensing (QS) which is encoded by the luxS family gene and curli by two operons, namely the csgBA operon. The purpose of the study is to detect the effects of 2 virulence genes (csgD and luxS) on biofilm-forming UPEC associated with UTI. As many as 76 UPEC isolates were collected from the clinical microbiology laboratories and the biofilm development was analyzed using the crystal violet method on microplate 96 wells. Using PCR assay, the two studied genes (csgD and luxS) were determined to be present in the isolates. UPEC isolates the bacteria-produced biofilms (90.80%) and nonproducers (9.20%). Most UPEC bacteria (97.36%) are known to be positive for csgD and luxS gene, while the others (92.10%) are known to be positive for the luxS gene. The highest proportion of the genes expressed in this study is followed by the presence of a relationship between the ability to produce biofilm and the presence of the genes under investigation, which is followed by all UPEC strains that cause UTI in humans.
THE EFFECT OF BREED AND BODY CONDITION SCORE ON OVARIAN WEIGHT, NUMBER OF FOLLICLES, AND OOCYTE QUALITY IN RETIRED COWS Putri, Natalie Lovena Pramono; Ikhsan, Dilla Amalia Bilqis; Raharjo, Dadik; Khairullah, Aswin Rafif; Lestari, Tita Damayanti; Mustofa, Imam; Rimayanti, Rimayanti; Safitri, Erma; Amrullah, Muhammad Fajar; Ilmi, Laily 'Ulya Nurul; Pasang, Gabriel Sampe; Supriyadi, Supriyadi
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.44392

Abstract

The aim of this study was to determine whether breed and body condition score (BCS) can influence ovarian weight, follicle number, and oocyte quality. This study used 90 ovaries from 15 Friesian Holstein crossbreed, 15 Ongole crossbreed, and 15 Limousin crossbreed cows obtained from an abattoir. Before the ovaries were taken, the BCS of the cows was determined. The BCS system used in this study is BCS with a scale of 1 to 5. Ovaries were weighed, follicles were measured and grouped by size (2 mm, 2-6 mm, 6 mm) then counted, oocytes were aspirated from the follicles then observed under a microscope. Oocyte quality was observed and then grouped into grade A, B, C, D based on cumulus oocyte complex (COCs). Data were analyzed using two way ANOVA and chi square test. The results of the analysis showed that breed did not influence ovarian weight, but influenced the number of follicles, and oocyte quality. In Friesian Holstein crossbreed cows, BCS affects ovarian weight, follicle number, and oocyte quality. In Ongole crossbreed cows, BCS had no effect on ovary weight, but did affect follicle number and oocyte quality. In Limousin crossbredcows, BCS had no effect on ovary weight and follicle number, but did affect oocyte quality.
IMPROVEMENT DAIRY CATTLE BARN MANAGEMENT AFTER FOOT AND MOUTH DISEASE (FMD) OUTBREAK IN MEDOWO, KEDIRI Permatasari, Dian Ayu; Raharjo, Dadik; Effendi, Mustofa Helmi; Budiarto, Budiarto
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 2 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i2.2025.260-270

Abstract

The Foot and Mouth Disease (FMD) outbreak that began in 2022 caused significant losses for dairy farmers in Medowo Village, Kandangan District, Kediri Regency—impacting both livestock health and farmers' economic stability. To address these impacts, a community service program was carried out to improve farmers' knowledge and skills in dairy cattle housing management and biosecurity implementation. The program included training sessions, educational outreach, and technical assistance for 31 farmers from KUD Kertajaya. Evaluation was conducted through pre-test and post-test instruments to measure the effectiveness of the intervention. Pre-test results showed that only 6.5% of participants were in the "good–very good" category in terms of understanding FMD and livestock management. After the intervention, post-test results showed a significant increase, with 87% of participants reaching the "good–very good" level. Additionally, in terms of practical application of cattle management practices, 100% of participants demonstrated excellent understanding after the program. This intervention proved that an educational approach combining outreach and hands-on practice can significantly enhance farmers' awareness and skills. The improvement is expected to contribute to better livestock productivity and more effective disease control. The activity also involved undergraduate students as part of the Merdeka Belajar Kampus Merdeka (MBKM) program, fostering collaboration between academia and the farming community to strengthen local capacity.
Gecko (Gekko gecko) Captive Breeding Development Strategy In East Java Bugiono, Bugiono; Raharjo, Dadik; Hidanah, Sri; Soeharsono, Soeharsono; Ardianto, Ardianto; Setyawan, Boedi
El-Hayah:Jurnal Biologi Vol 10, No 1 (2024): EL-HAYAH (VOL 10, NO 1 September 2024)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v10i1.31888

Abstract

Geckos (Gekko gecko) are widely used in traditional Chinese medicine and are reported to have anti-tumor properties. Approximately 93% of reptiles exported from Indonesia come from the wild. The direct and continuous capture of geckos in nature will threaten gecko populations in the future. As an effort to control the possible threat of extinction, the Indonesian government encourages the utilization of this animal through captive breeding activities. The existence of gecko (Gekko gecko) captive breeding units in East Java has not been widely developed and still uses conventional and semi-natural patterns. Periodic evaluations need to be carried out to determine whether or not this breeding activity is feasible and what the development strategy is in the future. This study aims to analyze the strengths, weaknesses, opportunities and threats in order to formulate a business development strategy for gecko breeding in East Java. This research uses a survey method by analyzing and describing the problems in the field, while the technical analysis uses internal factor analysis (IFE matrix), external factor analysis (IFE matrix) and SWOT analysis. Based on the results of the SWOT analysis, the development strategy of the gecko (Gekko gecko) breeding business in East Java includes: maintaining market confidence and maintaining quality and production stability; adjusting government regulations related to gecko trade governance; developing effective breeding methods; adjusting government regulations related to breeding governance; establishing market strategies and production stability to suppress illegal trade; and researching new market potential for gecko trade development
Description of antemortem inspection of cattle at the Dimoro slaughterhouse, Blitar City Hasibuan, Sandi Aziz; Lokapirnasari, Widya Paramita; Triakoso, Nusdianto; Budiarto, Budiarto; Warsito, Sunaryo Hadi; Raharjo, Dadik
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78414

Abstract

The outbreaks of Foot and Mouth Disease (FMD) and Lumpy Skin Disease (LSD) in early 2024 significantly impacted livestock health conditions in Blitar City and led to an increase in emergency slaughter cases. The Dimoro Slaughterhouse plays a crucial role in ensuring the safety of animal-derived food through the implementation of antemortem inspections. This study aims to evaluate the health status of beef cattle and identify pathological conditions detected during antemortem inspections at the Dimoro Slaughterhouse. The research used a quantitative descriptive method with direct observation of 40 cattle during the period of June 13–19, 2025. Clinical examinations were conducted on the head, neck, thorax, abdomen, anus, and limbs. The results showed that most cattle were male (34 heads) and predominantly of the Limousin breed (19 heads). No clinical symptoms of FMD, LSD, fever, or other disorders warranting slaughter rejection were found. Mild findings such as dirt around the anus were recorded in some individuals. Overall, the antemortem inspection was carried out effectively and contributed to ensuring the safety of meat products for public consumption.
Occurrences Salmonella sp. and Escherichia Coli in Bulk and Packaged Chicken Sausages in Surabaya, Indonesia Nur, Deana Fyra Adi; Yulistiani, Ratna; Rosida, Dedin F.; Raharjo, Dadik
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 6 No. 2 (2022)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v6i2.99

Abstract

Salmonella sp. and Escherichia coli are pathogenic bacteria that cause foodborne diseases that often contaminate food and are harmful to human health. Sales of unpackaged (bulk) sausages accompanied by poor hygiene and environmental sanitation conditions have a high potential for Salmonella and Escherichia coli contamination. The purpose of this study was to compare the contamination level of bacterial, Salmonella sp. and Escherichia coli in bulk and packaged chicken sausages as well as to determine the relationship between the hygiene and sanitation of traders and the level of bacterial contamination, Salmonella sp. and Escherichia coli contamination in chicken sausages sold at traditional markets in Surabaya, Indonesia. This research is a cross-sectional study and purposive sampling method. The results showed that the level of bacterial contamination in the bulk chicken sausage was higher (5.98 Log CFU/g) than in packaged chicken sausage (4.83 Log CFU/g). Salmonella sp. contamination in the bulk chicken sausage was higher (44.44%) than in packaged chicken sausage (10.00%) and Escherichia coli contamination in the bulk chicken sausage was higher (22.22%) than in packaged chicken sausage (20.00%). There is a significant relationship between the hygiene and sanitation of traders with the contamination level of bacteria, Salmonella sp., and Escherichia coli in bulk and packaged chicken sausages.
Assessment of the Rapid Immunochromatographic Test as a Diagnostic Tool for Norovirus Related Diarrhea in Children Ranuh, Reza Gunadi; Athiyyah, Alpha Fardah; PA, Deanty Ayu; Darma, Andy; Raharjo, Dadik; Shirakawa, Toshiro; Sudarmo, Subijanto Marto
Folia Medica Indonesiana Vol. 55, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In developing countries, Norovirus is the second-leading cause of acute diarrhea, after rotavirus. The approved gold standard method for diagnosis of norovirus infection is RT-PCR. The rapid immunochromatographic test is a novel and expedient method for diagnosing norovirus that is relatively affordable. However, the use of the rapid immunochromatographic test remains controversial because of its accuracy. This study aimed to explore whether the rapid immunochromatographic test could be used for diagnosing norovirus-related diarrhea in children. Rapid immunochromatographic test (QuickNaviTM-Norovirus2) and RT-PCR on stool samples was used to diagnose norovirus. Stool samples were obtained from pediatric patients aged between 1 and 60 months who had diarrhea and were admitted to the pediatric ward at Dr. Soetomo General Hospital Surabaya, between April 2013 and March 2014. Ninety-four subjects provided stool samples that were tested using QuickNaviTM-Noro2 and RT-PCR. Using the test, 64 samples tested positive for norovirus and 30 tested negatives. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the rapid immunochromatographic test were consecutively 90.3%, 42.9%, 43.8%, 90%, and 58.5%. RT-PCR was used to test all samples to assess the accuracy, which showed that one from 31 samples contained the GI strain (1.1%), while 30 samples (32%) contained the GII strain. This study definitively establishes that the rapid immunochromatography test is not sufficiently accurate for use as a screening or diagnostic tool in norovirus-related diarrhea cases in children.
STRENGTHENING ONE HEALTH IMPLEMENTATION AT AIRPORTS THROUGH CROSS-SECTORAL BIOSECURITY MANAGEMENT Permatasari, Dian Ayu; Raharjo, Dadik; Effendi, Mustofa Helmi; Budiarto; Suwanti, Lucia Tri
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 10 No. 1 (2026): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This community service program was directed at enhancing the implementation of the One Health approach at airports through cross-sectoral biosecurity management. Airports are important gateways where close interactions among humans, animals and the environment create a high potential for the emergence of zoonotic diseases. The activities were conducted at Sultan Hasanuddin International Airport and involved airport authorites, airport operational staff, quarantine officers, public health agencies, and academic collaborators. The program include socialization sessions, focus group discussions, workshops, and mentoring activities aimed at  strengthening stakeholders understanding of One Health principles and improving coordination mechanisms in biosecurity management. The results indicated improved cross-sectoral coordination, increased awareness of zoonotic risks management, and greater preparedness among participants to implement standardized biosecurity practices. The academic capacity building activities supported participants readiness for scientific publication and improved citation strategies. Overall, the program demonstrates that a cross-sectoral biosecurity management approach can serve as an effective strategy to strengthens the implementation of One Health at airports and promote sustainable public health protection.
Co-Authors Adiana Mutamsari Witaningrum Agnes Theresia Soelih Estoepangestie Aldin Akbar Rahmatullah Alfiyatus Sholichah Alpha Fardah Athiyyah Amrullah, Amirul Muslim Amrullah, Muhammad Fajar Andy Darma Budiarto Budiarto Budiarto Bugiono, Bugiono Caska - Cindy Ercha Aulia Putri Deana Fyra Adi Nur Dedin F. Rosida Dian Ayu Permatasari Didik Handijatno Dinda Alvianita Sabrina Endang Suprihati Eriza Rosalina Rochmah Erma Safitri ERMA SAFITRI Erma Safitri Fedik Abdul Rantam Hasibuan, Sandi Aziz Hidanah, Sri I Gusti Made Reza Gunadi Ranuh Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Jariyah Kartini Khairullah, Aswin Rafif Lucia Tri Suwanti, Lucia Tri Masfufatun Masfufatun Mohammad Auzaie Afandi Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nenny Harijani Ni Made Mertaniasih Nunuk Dyah Retno Lastuti Nur, Deana Fyra Adi Nusdianto Triakoso Nuurin Ajrin Karim PA, Deanty Ayu Pasang, Gabriel Sampe Poedji Hastutiek Prawasa, Ramadhiniyanti Putri Alif Pudji Srianto Putri, Natalie Lovena Pramono Ratna Yulistiani Rimayanti Rimayanti Rimayanti Rimayanti Rimayanti Rini Purbowati Rizky Fatkhur Rohman Rosida, Dedin F. Sakran, khawla Abdullah Setyawan, Boedi Shirakawa, Toshiro Sintya Kumalasari Wibowo Sintya Kumalasari Wibowo Soeharsono Soeharsono Sofia Wardhani Sri Alam Syah Lihan Candra Asi Sri Hidanah Sri Lestari Utami Sudarmo, Subijanto Marto Supriyadi Supriyadi Tacharina, Martia Rani Tita Damayanti Lestari Toshiro Shirakawa Tyasningsih, Wiwiek Ulya Sarofa Ulya Sarofa Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari