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Putri Pratiwi
Universitas Indonesia
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Journal : SAINS DAN MATEMATIKA

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TOTAL FENOLAT DAN FLAVONOID DARI EKSTRAK DAN FRAKSI DAUN KUMIS KUCING (Orthosiphon stamineus B.) JAWA TENGAH SERTA AKTIVITAS ANTIOKSIDANNYA Pratiwi, Putri; Suzery, Meiny; Cahyono, Bambang
JURNAL SAINS DAN MATEMATIKA Volume 18 Issue 4 Year 2010
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

ABSTRAK---Orthosiphon stamineus B (Indonesia: Kumis Kucing) merupakan salah satu tumbuhan sangat popular sebagai sumber pengobatan herbal, pada umumnya dikumpulkan dari pulau Jawa. Aktivitas biologis, terutama akivitas antioksidan yang ditunjukkan oleh tanaman ini diduga disebabkan olehsenyawa golongan fenolat, khususnya senyawa flavonoid. Oleh sebab itu, penelitian ini bertujuan menentukan total fenolat, total flavonoid dan aktivitas antioksidan dari ekstrak metanol beserta fraksi fraksinya dari tanaman kumis kucing yang tumbuh di Indonesia, khususnya di daerah Bandungan, Kabupaten Semarang, Provinsi Jawa Tengah, perlu dilakukan. Secara garis besar, penelitian dibagi menjadi empat tahap, yakni perolehan total ekstrak metanol, diikuti dengan tahap fraksinasi melalui gradien pelarut, kemudian analisis total fenolat dan flavonoid, dan diakhiri dengan analisis aktivitas antioksidan terhadap tiap-tiap ekstrak. Analisis total fenolat dilakukan dengan metode Folin-Ciocalteu, analisis flavonoid dilakukan berdasarkan cara kerja Rohman, dan aktivitas antioksidan ditentukan dengan metode peredaman radikal DPPH.. Hasil penelitian menunjukkan bahwa kandungan total fenolat dan flavonoid terbesar berada dalam ekstrak etil asetat berturut-turut sebesar 559 mg asam galat ekuivalen/g ekstrak dan 3550 mg kuersetin ekuivalen/g ekstrak. Aktivitas antioksidan tertinggi juga dimiliki oleh ekstrak etil asetat dengan nilai EC50 sebesar 51,02 μg/mL. Nilai aktivitas antioksidan yang ditunjukkan oleh semua fraksi dalam daun kumis kucing sangat memungkinkan bahan ini dapat digunakan sebagai sumber antioksidan.Kata Kunci: kumis kucing, Orthosiphon stamineus, total fenolat, total flavonoid, antioksidan ABSTRACT---Orthosiphon stamineus B (Lamiaceae) is a popular medicinal herb in South-east Asia, it was originated in Java Island and is well known by name of “Kumis Kucing.”. The biological activityof this plant, especially the antioxidant activity, is caused by the presence of compounds from phenolic group, especially come from flavonoid compounds. Therefore, the aim of this research is to determine total phenolic, total flavonoid and antioxidant activity analysis of methanol extract and its fractions of Orthosiphon stamineus B from Indonesia, especially Bandungan, Kabupaten Semarang, Jawa Tengah. The method of this research is consists of dried leaf extraction by methanolic, partition with solvent gradient, analysis of total phenolic and flavonoid content and antioxidant activity analysis. The total phenolic content analyzed by Folin-Ciocalteau method, total flavonoid content analyzed by Rohman method and antioxidantactivity analyzed by DPPH. radical scavenging method. The result showed that the highest phenolic and flavonoid content is in the ethyl acetate extract with value 559 mg gallic acid equivalent/g extract and 3550mg quercetin equivalent/g extract, respectively. The ethyl acetate has the strongest antioxidant activity with51.02 μg/mL of EC50 value. The finally antioxidant activity is shown in all extract of Indonesian Orthosiphonstamineus B can be used as antioxidant source.Keywords: Orthosiphon stamineus, kumis kucing, total phenolic, total flavonoid, antioxidant activity
Co-Authors Adelina, Dian Aga Pratama Alfiati Syafrina, Alfiati Alfith Alfith Ali Amran Ana Witra Anastasya, Anastasya Andi Sukainah Anggraini , Puput Anita Desiani Anrinal Anrinal Archaqie, Haikal Nur Rachmanrachim Arfidian Rachman Aritonang, Vicky Robianto Arni Arni Ashari, Lucky Asmara Yanto Asmawati asriati, weni widya Astuti - Bambang Cahyono Bambang Suprihatin Basyarul Ulya boby rahman, boby Cantika, Amelya Delvis Agusman Dwifa, Dima Eko Bambang Murdiansyah Erismar Amri Fachrurrozi, Ahmad Fadila, Sri Fitra Soni, Idel gustio mitro Hafni Hafni Hendriwan Fahmi Herlinda Herlinda, Herlinda Herry Cahyana Ida Nuraida Idris, Maulana Fahmi Ilna, Ilna Irada Sinta Irnanda, Tanti Israwati., Israwati Jasmi Jasmi Jasrudin, Jasrudin Julia, Nur Tri Kalsum, Ummul Rabbayani Khasanah, Eka Robiul Kossay, Methodius Lidya Novita Sari M. Yahya Mastariyanto Perdana Mayangsari, Mayangsari Meiny Suzery Millisa, Cut Mudassir Mudassir, Mudassir Muhammad Syaifullah, Muhammad Neng Sari Nofriady Handra, Nofriady Nuri Gustia Nursuhaela Nurzal, Nurzal Pongdatu, Merry Putri, Yecha Febrieanitha Rahmat, Alibin Rahmi Syafriyeti Ramadhan, Akbar Ramadhani Yusuf , Raya Rifaldy Rizky, Muhammad Romi Romi, Romi Rozi Saferi, Rozi Ruswana, Angra Meta Saputra, Fiko Saputra, Georgery Sartika Sartika Silvi Trisna Slamet Hariyadi Sopiandy, Dede Sova, Sazkia Gaisa Suparman, Sri Rahayu Suwardi Talib, Nur Qalbi Tri Maryanto Umi Kalsum Wahiyuni, Aning Wahyuni, Asti Yani, Sri Astri Yanto, Subari Yulianti, Lusi yunasfi yunasfi Yuniar, Fira Yurian , Frizky Yuwazama, Zido Zakia, Alya