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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Natur Indonesia Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Journal of Aquatropica Asia Jurnal Kelautan Tropis Panrita Abdi - Jurnal Pengabdian pada Masyarakat JFMR (Journal of Fisheries and Marine Research) JRST (Jurnal Riset Sains dan Teknologi) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pengelolaan Perairan Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Natural Pigments Aquacultura Indonesiana Journal of Tropical Marine Science Indonesian Journal of Mollusk JURNAL BIOLOGI PAPUA Journal of Fisheries & Marine Jurnal Ilmu Kelautan Lesser Sunda

Delianis Pringgenies

Jurusan Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro Jl. H. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275

Author-ID : 347619

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Social Sciences Other

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Pengaruh Pemberian Ekstrak Stichopus hermanii Semper, 1868 (Stichopodidae, Holothuroidea) terhadap Jumlah Total Hemosit Litopenaeus vannamei Boone, 1931 (Penaeidae, Crustacea) Ni'mah, Ulin; Pringgenies, Delianis; Santosa, Gunawan Widi
Journal of Marine Research Vol 10, No 3 (2021): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v10i3.31112

Peningkatan imunitas udang vaname dengan menggunakan imnostimulator merupakan salah satu upaya untuk mencegah kegagalan panen pada budidaya udang vaname. Ekstrak teripang emas memiliki senyawa yang berperan sebagai peningkat imun. Beberapa senyawa yang terkandung pada teripang emas yaitu saponin dan steroid. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak teripang emas terhadap jumlah total hemosit Litopenaeus vannamei dan konsentrasi ekstrak teripang emas yang paling tepat untuk meningkatkan jumlah total hemosit udang vaname. Metode yang digunakan adalah metode eksperimen laboratoris dengan perlakuan konsentrasi ekstrak teripang emas yang ditambahkann pada pakan udang komersil yaitu 0 ppm; 40 ppm; 80 ppm; 120 ppm. Hasil penelitian jumlah total hemosit udang vaname terjadi peningkatan akibat pemberian ekstrak teripang emas dibandingkan kontrol. Peningkatan tersebut terlihat pada jumlah total hemosit udang vaname yang diberi ekstrak teripang emas dengan konsentrasi 40 dan 120 ppm pada hari ke-8. Pemberian ekstrak teripang emas dengan konsentrasi 120 ppm memberikan hasil terbaik yaitu jumlah total hemosit sebanyak 1,18x106 sel/mL, dan untuk kelangsungan hidup udang vaname yang diberikan ekstrak teripang emas dan kontrol memiliki persentase sebesar 100%. Kesimpulannya adalah pemberian ekstrak teripang emas berpengaruh terhadap jumlah hemosit udang vaname.Â Â Increased immunity of vaname shrimp by using imnostimulator is one of the efforts to prevent crop failure in the cultivation of vaname shrimp. gold sea cucumber extract has a compound that acts as an immune enhancer. Some of the compounds contained in Stichopus hermanii are saponins and steroids. The purpose of this study was to determine the effect of gold sea cucumber extract on the total amount of vaname shrimp haemocyte and the most appropriate concentration of golden sea cucumber extract to increase the total amount of vaname shrimp haemocyte. The method used was a method of laboratory experimentation with the treatment of the concentration of Stichopus hermanii extract added to commercial shrimp feed that is 0 ppm; 40 ppm; 80 ppm; 120 ppm. The results of the study the total amount of shrimp haemocyte Litopenaeus vananamei increased due to administration of Stichopus hermanii extract compared to control. The increase was seen in the total amount of vaname shrimp haemocyte given gold sea cucumber extract with concentrations of 40 and 120 ppm on day 8. Administration of gold sea cucumber extract with a concentration of 120 ppm gives the best result that is the total amount of haemocyte as much as 1.18x106 cells / mL, and for the survival of Litopenaeus vannamei given extract gold sea cucumber and control has a percentage of 100%. The conclusion is that the administration of gold sea cucumber extract affects the amount of haemocyte shrimp vaname.

FENOMENA BIOLUMINENSENSI CUMI-CUMI (Loligo duvauceli) BERASAL DARI BAKTERI SIMBION Delianis Pringgenies
Jurnal Harpodon Borneo Vol 5, No 1 (2012): Volume 5 No 1 April 2012
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v5i1.83

Tujuan penelitian adalah mengungkap simbiosis antara bakteri dengan organ cahaya cumi-cumi pada proses bioluminesensi dengan asumsi bahwa: kemampuan pencahayaan tidak dimiliki oleh organisme cumi-cumi itu sendiri; terjadi simbiosis antara cumi-cumi dan bakteri; dan proses simbiosis antara bakteri dengan cumi-cumi terjadi secara horizontal, yakni bakteri berasal dari luar dan besimbiosis pada cumi-cumi setelah menetas dari telur. Pemecahan masalah dalam penelitian ini dilakukan melalui aspek cumi-cumi dan bakteri, dan kemudian dianalisis tentang ketergantungan kedua organisme tersebut dengan cara: histologi organ sebagai sumber cahaya dengan metode mikroskop elektron transmisi (TEM ) pada juvenil dan dewasa cumi-cumi. Hasil penelitian menunjukkan bahwa jenis cumi-cumi yang memancarkan cahaya memiliki sepasang organ cahaya menempel pada dorso-lateral kantung tinta. Organ cahaya sudah terbentuk sempurna pada cumi-cumi juvenil umur satu hari dan selanjutnya membesar mengikuti pertumbuhan cumi-cumi. Bakteri di dalam kantung bakteri merupakan bakteri yang hidup secara ektraselular dan kerapatannya di dalam kantung meningkat sejalan dengan umur cumi-cumi juvenil sampai dewasa. Dari hasil analisis histologi kantung organ cahaya, didapat bahwa kantung organ cahaya cumi-cumi juvenil umur satu hari belum mengandung bakteri. Bakteri mulai dijumpai dalam koloni kecil pada cumi-cumi umur dua hari dan pada cumi-cumi yang lebih besar serta dewasa. Sel-sel bakteri terdapat dalam kerapatan yang padat di dalam lumen kantung hewan dewasa. Kehadiran bakteri pada organ cahaya cumi-cumi terjadi secara horizontal yang berarti bahwa bakteri berasal dari luar lalu masuk bersimbiosis ke dalam organ cahaya melalui saluran bersilia kantung setelah cumi-cumi menetas. Bioluminesensi yang terjadi dimanfaatkan cumi-cumi untuk strategi pertahanan melalui penyamaran dan mekanisme komunikasi antar lawan jenis. Key words : Bakteri, simbion, cumi, organ cahaya

The Bioluminescence Phenomenon of Lomek Fishes (Harpadon nehereus) with their Luminous Bacteria Kartika Dewi; Delianis Pringgenies; Haeruddin Haeruddin; Sakti Imam Muchlissin
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 3 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Lomek fish (Harpadon nehereus) is one of the most popular fish in Riau, which is widely distributed along the sea of Tanjung Balai Karimun, Riau Archipelago, Indonesia. Based on direct observation, this fish emits light when uncovered in open air and caused a phenomenon which called as bioluminescence. The purposes of this research were to reveale the cause of luminosity of the fish and to investigate the luminosity activity. This research was conducted in several stages ie; sampling and sample preparation, observation of bioluminescence activity, and isolation of luminous bacteria. The results showed that the bioluminescent of lomek fish occurred around the surface of the body. After 10 to 11 hours, the luminosity can be observed up to the innermost parts of the body. It is revealed that the bioluminescent of this fish is simply due to the presence of luminous bacteria on surface of its body. The bacteria could emit light on primary isolation after 48 h incubation and the light last for 8 h ahead.

STUDY OF NUTRITIONAL CONTENTS OF SEA URCHIN GONAD FROM DRINI BEACH, GUNUNG KIDUL, YOGYAKARTA Delianis Pringgenies; Richardus Mahatmada Indrajati; Ali Djunaedi
Jurnal Kelautan Vol 13, No 3: Desember (2020)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v13i3.7808

ABSTRACTThis paper aims to determine the types and contents of protein, fatty acids, amino acids and protein in the gonads of Arbacia lixula, Colobocentrotus atratus, Heterocentrotus trigonarius and Echinotrix diadema from the waters of Drini, Gunung Kidul, Yogyakarta. Kjedal method was used to analyze the protein content, while GC and HPLC methods were employed to analyze amino acids in this study. The results showed that the protein contents of each sample, consequently from highest to lowest, were Heterocentrotus trigonarius (13.3 %), Echinotrix diadema (10.86), Colobocentrotus atratus (10.41) and Arbacia lixula (9.90%). Amino acids analysis from all identified both saturated fatty acids, consisting of lauric acid, myristic acid, palmitic acid, stearic acid, arachidonic acid, lignoceric acid, as well as non-saturated fatty acids, consisting of palmitoleic acid, oleic acid, linoleic acid, erucic acid, EPA, and DHA. The highest contents of non-saturated fatty acids were identified in Colobocentrotus atratus (434.14 mg/100g) and the lowest content in Arbacia lixula (197.71 mg/100g). The highest percentage of essential fatty acids was found in Heterocentrotus trigonarius (0.29%), whereas the lowest was found in Echinotrix diadema (0.19 %). It is concluded that the gonad of Heterocentrotus trigonarius showed the highest protein and essential fatty acids contents. This study also found that Colobocentrotus atratus sea urchin gonads possess the highest content of non-saturated fatty acids (434.14 mg/100g). Keywords: amino acid, fatty acid, protein, sea urchin gonad.

ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS – INDONESIA Delianis Pringgenies; Ali Ridlo; Nerva Sembiring
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

The study aims to explore the antibacterial activity of Stichopus vastus against pathogenic MDR bacteria. Analysis of samples of sea cucumbers included extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometry (GC-MS), the extraction process is carried out by solid-liquid extraction method. Fractionation was done with Open-Column Chromatography (OCC). Sensitivity test of bacteria was done using an agar diffusion method according to the Kirby-Bauer (Ref). The study revealed that from 5 species MDR bacteria, which are Coagulant negative stapylococi (CNS), E. coli, Enterobacter 5, Klebsiella sp. dan Pseudomonas sp. There are two MDR bacteria had the most sensitive responses by the extract of sea cucumber, which were Enterobacter-5 and Klebsiella sp. The two bacteria were tested against five bioactive fractions obtained from OCC. Fraction criteria-2 had the highest antibacterial activity against Enterobacter-5 and Klebsiella sp, with serial concentration of 20 µg ∙ disc–1, 40 µg. disc–1 and 80 µg. disc–1. Largest inhibition zone were obtained from 80 µg. disc–1 againts the two bacteria were (14.73 ± 0.48) mm and (11.22 ± 0.85) mm respectively. GC-MS Analysis revealed that fraction criteria-2 had (or consisted of) cyclohexhane, ethanol, butanoic and pentanoic acids. Keywords: antibacterial activity, multi drug resistance (MDR), sea cucumber, Stichopus vastus

Morfologi dan Anatomi Cumi-Cumi Loligo duvauceli yang Memancarkan Cahaya Esti Rudiana; Delianis Pringgenies
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 2 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Setiap makhluk hidup di alam harus mempunyai strategi agar dapat lulus hidup dan strategi tersebut berbedabeda untuk setiap individu. Salah satu strategi cumi-cumi dalam mempertahankan diri dengan cara memancarkan cahaya, oleh karena itu ada beberapa jenis cumi-cumi yang memiliki organ cahaya. Tujuan penelitian ini adalah mengkaji morfologi dan anatomi cumi-cumi Loligo duvauceli yang dapat memancarkan cahaya. Pemecahan masalah dalam penelitian ini dilakukan melalui aspek cumi-cumi yang ditangkap langsung darilait Jepara dan dianalisis morfologi dan anatominya. Hasil penelitian menunjukkan bahwa jenis cumi-cumi yang memancarkan cahaya di perairan Jepara dan sekitarnya adalah Loligi duvauceli yang memiliki sepasang organ cahaya menempel pada dorso-lateral kantung tinta. Organ cahaya cumi berbentuk bulat, sebagian terdapat pada permukaan dan sebagian terbenam pada dinding kantung tinta. Organ cahaya terdiri atas lensa yang terletak pada permukaan luar kantung tinta, dan kantung organ cahaya (terbenam pada dinding kantung tinta) yang mempunyai saluran penghubung kantung dengan rongga mantelKata kunci: cumi-cumi (Loligo duvauceli), morfologi, anatomi, organ cahayaEvery organism in nature has a strategy in order to survive and that strategy may be deferent on each organism. Squids escape from predators by emitting lights; therefore, squids have to develop an organ in order to be able to emit light. Squids were collected from Jepara water and adjacent, the observation was done by analyzing of morphology and anatomy of squid. Results from identification of squids live in Jeparawaters and the adjacent is Loligo duvauceli. This species has a pair of light organs attached at the dorsalateral of the ink sac. The light organ of the squid has a rounded shape, which is partly emerge on the surfaceand the other part was submerge in the wall of the ink sac. Light organ consists of a lens situated on the outer part of the ink sac and it has a connecting canal to the mantel holeKey words : squid (Loligo duvauceli), morphology, anatomy, light organ

Amino Acid Absorption by Tiger Grouper Fish (Epinephelus fuscoguttatus) Larvae. (Absorbsi Asam Amino oleh Larva Ikan Kerapu Macan (Epinephelus fuscoguttatus)) Ambariyanto Ambariyanto; Ali Djunaedi; Nur Taufiq S.P.J.; Pribadi Rudhi; Pringgenies Delianis
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 4 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Ikan Kerapu merupakan salah satu ikan unggulan yang ditargetkan sebagai komoditi eksport Indonesia. Usaha budidayanya saat ini sangat terganggu dengan tingginya mortalitas pada stadia larva. Usaha untuk mengatasi masalah tersebut adalah dengan menggunakan obat seperti antibiotik yang ternyata tidak membuahkan hasil maksimal tetapi justru menimbulkan resistensi beberapa jenis bakteria. Salah satu aspek yang belum pernah dilihat dalam rangka mengatasi masalah ini adalah dengan mengusahakan percepatan pertumbuhan pada stadia larva sehingga akan lebih mampu menghindari dari beberapa penyebab mortalitas. Salah satu sumber energi yang terdapat dalam perairan namun dalam jumlah yang tidak besar adalah dissolved organic matter (DOM). Penelitian ini ditekankan untuk melihat kemampuan larva ikan Kerapu dalam memanfaatkan DOM (digunakan asam amino terlarut ;ATT) yang terdapat di air laut. Sebanyak 16 (enam belas) jenis asam amino yang terdiri dari tiga klas yakni neutral, basic, dan acidic digunakan dalam penelitian ini. Sedangkan larva ikan Kerapu yang digunakan berumur 2 hari. Hasil penelitian menunjukkan bahwa larva ikan Kerapu menyerap seluruh jenis asam amino baik neutral, basic, dan acidic. Namun jenis asam amino yang diserap adalah glutamat, histidin, lisin, serin, metionin, tritopan dan iso leusin. Sedangkan yang paling banyak diserap oleh larva ikan ini adalah lisin. Hal yang menarik dalam penelitian ini adalah terdapat beberapa jenis asam amino yang diduga justru dikeluarkan oleh larva ikan tersebut yakni glysin, alanin, tyrosin, valin, phenil alanin dan leusin. Penyerapan beberapa jenis asam amino ini diduga dimanfaatkan oleh larva ikan Kerapu dalam proses pertumbuhannya. Kata kunci : asam amino terlarut, larva, Kerapu Macan, Epinephelus fuscoguttatus Kerapu (grouper fish) is known as an important and highly economic value fish and a good candidate for major export commodity for Indonesia. However, there is an important problem faced by its cultivation i.e. high mortality rate at larva stage. Many different efforts have been done to overcome this problem mainly by using drugs and antibiotics, which have caused another problem i.e. bacteria resitance. One aspect that has not been widely investigated is by increasing its growth rate so that the larvae will have the ability to avoid mortality, such as by utilising dissolved organic matter (DOM) which naturally occur in the environment. This research investigates the question whether Kerapu fish larvae have the ability to absorb DOM (in this case disolved free amino acids; DAA) as well as the preference and the rate of absorbsion. There were 16 species of DAA used in this experiment which consist of three classes i.e. neutral, basic, and acidic. Two days old larvae were used in the experimant. The results showed that Kerapu larvae absorbed all classes of amino acids, although not all amino acids given being absorbed but only glutamine, histidine, lysin, serine, triptophan, metionine and iso leusine. While the most absorbed amino acids was lysine. One interesting results showed that the larvae secrete several amino acids i.e. glysine, alanine, tyrosine, valine, phenil alanine and leusine. The absorbsion and secretion of amino acids were possibly related to its metabolic processes within the larvae in relation to growth processes. Keywords: dissolved free amino acids, DAA, larvae, Kerapu, Epinephelus fuscoguttatus

Antibacterial Activity Test of Nudibranches Polka - Dot (Jorunna funebris) (Gastropods : Molusc) Extract Against Multi(Aktivitas Antibakteri Ekstrak Nudibranch Polka-Dot (Jorunna funebris) (Gastropoda : Moluska) Terhadap Bakteri Multidrug Resistant (MDR)) Delianis Pringgenies; Masnah Jumiati; Ali Ridho
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 4 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Terjadinya resistensi antibiotik menjadi permasalahan dalam dunia kesehatan. Peningkatan kemampuan patogen dalam menahan efek obat menyebabkan timbulnya resistensi. Beberapa bakteri patogen pada manusia dilaporkan telah mengalami resistensi terhadap lebih dari satu kelas antibiotik. Untuk mengatasi permasalahan tersebut, maka perlu dilakukan pencarian senyawa antibiotik baru yang lebih efektif dalam mengatasi permasalahan bakteri Multi-drug Resistant (MDR). Metabolit sekunder yang diproduksi oleh invertebrata laut mempunyai prospek sebagai bahan obat dari laut. Nudibranch diduga mampu menghasilkan metabolit sekunder sebagai mekanisme pertahanan diri. Tujuan dari penelitian ini adalah untuk mengetahui fraksi dari ekstrak nudibranch Jorunna funebris yang menunjukkan bioaktivitas terhadap bakteri Multi-drug Resistant (MDR). Proses ekstraksi dilakukan dengan metode maserasi. Fraksinasi dengan Kromatografi Kolom Terbuka (KKT). Uji aktivitas antibakteri menggunakan metode difusi agar. Analisis komponen senyawa dengan GC-MS. Hasil penelitian menunjukkan bahwa 8 fraksi ekstrak nudibranch J. funebris menunjukkan aktivitas antibakteri. Hasil uji aktivitas menunjukkan fraksi I paling aktif terhadap 5 bakteri uji yaitu Klebsiella, Pseudomonas, Escherichia coli, Enterobacter 5 dan Enterobacter 10 dengan rata-rata zona hambatan secara berurutan sebesar 12,78 mm; 12,51 mm; 15,47 mm; 14,09 mm dan 12,46 mm. Fraksi II paling aktif terhadap bakteri Coagulase Negative Staphylococcus dengan rata-rata zona hambatan sebesar 12,70 mm. Analisis GC-MS menunjukkan bahwa dalam fraksi II terdapat senyawa 1-oktadekanol yang berpotensi sebagai antibakteri. Kata kunci : nudibranch, Jorunna funebris, antibakteri, multi-drug resistant, 1-oktadekanol Emergence of antibiotic resistance become a problems on medical world. Increasing pathogen ability to hold the antibiotic effect caused resistance. Several human-patogen bacteria were resistance to one or more classes of antibiotics. To solve those problems, it is necessary to search new antibiotic compounds that more effective to solve the problem of Multi-drug Resistant (MDR). The secondary metabolite produced by marine invertebrates have prospect as a marine biomedical. Nudibranch may produce the secondary metabolite as chemical defense mechanism. The purpose of the research was to obtain fraction from extract nudibranch Jorunna funebris that capable of bioactivity for Multi-drug Resistant (MDR) bacteria. Extraction process was conducted with maceration method. Crude extracts were fractionated by Open Column Chromatography. Antibacterial activity test was done using disk diffusion method. Chemical compounds were analyzed with GC-MS. The result showed that 8 fractions of nudibranch J. funebris extract showed antibacterial activity. Activity test showed fraction I has ability to inhibit Klebsiella, Pseudomonas, Escherichia coli, Enterobacter 5 and Enterobacter 10 with average value of inhibitory zone was 12,78 mm; 12,51 mm; 15,47 mm; 14,09 mm and 12,46 mm. Fraction II has ability to inhibit Coagulase Negative Staphylococcus with mean inhibitory zone was 12,70 mm. GC-MS analyses showed 1-octadecanol from fraction II, that capable as antibacterial compound. Key words: nudibranch, Jorunna funebris, antibacterial, multi-drug resistant, 1-octadecanol

Marine bacterium Seonamhaeicola algicola strain CC1 as a potential source for the antioxidant carotenoid, zeaxanthin Tatas Hardo Panintingjati Brotosudarmo; Edi Setiyono; Koichiro Awai; Delianis Pringgenies
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 4 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.4.215--224

Currently, there are only six species in the genus Seonamhaeicola, i.e., Seonamhaeicola aphaedonensis, S. algicola, S. marinus, S. acroporae, S. maritimus, and S. sediminis. These bacteria have typical yellow or orange color. Among the identified strains, only S. marinus that had been reported to have a yellow polyene flexirubin pigment. However, the presence of carotenoid pigments has not been reported in this genus. Recently, we successfully isolated a new strain, S. algicola strain CC1, bacterium that was found in association with a red seaweed, Halymenia sp., collected from the coast of South Malang, Indonesia. The strain was grown well in the Zobell marine agar 2216E producing yellowish pigments. According to the 16S rRNA sequencing analysis and BLAST search, the strain is closely related to S. algicola strain Gy8, with 99.78% identity. The pigment composition was separated and analyzed by a high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) and the strain was found to produce zeaxanthin as the major component, which appeared at a retention time (tR) of 28.89 min, showing a typical mass spectrum with a molecular ion at m/z 568.5 [M]+ and four product ions at m/z 261.4 [M−307]+, 476.6 [M−92]+, 429.3 [M−139]+, and 536.5 [M− 32]+. Other carotenoids, including zeaxanthin cis isomers, β-cryptoxanthin, β-carotene cis isomer, and β-carotene, are as minor components. The novel and noteworthy finding of this report is the identification of a Seonamhaeicola species that produces carotenoids and can be used as a source of zeaxanthin.

Isolasi dan Determinasi Bakteri Luminesensi yang Bersimbiosis pada Cumi-cumi Loligo duvauceli Delianis Pringgenies; Sri Sejati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 1 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Bioluminisensi merupakan penomena alam, yaitu cahaya yang ditimbulkan oleh suatu organ sebagai hasil dari reaksi kimia yang melibatkan tiga komponen, yakni luciferin (substrat), luciferase (enzim) dan molekul oksigen. Hasil penelitian menunjukkan bahwa bakteri simbiosis pada organ luminisensi cumi L. duvauceli adalah bakteriluminisen dari jenis bakteri Photobacterium phosphoreum dan bakteri memancarkan warna biru. Jenis bakteri ini merupakan bakteri yang paling terang memancarkan cahaya di bandingkan dengan jenis bakteri luminisensi lainnya. Hasil penelitian terhadap jumlah total bakteri memperlihatkan bahwa bakteri dapat memancarkan cahaya pada konsentrasi 4,6. 109 CFU/ml dengan diameter koloni bakteri pada 0,075 cm, sedangkan pada konsentrasi lebih rendah yaitu 2,0. 104 dengan diameter koloni bakteri 0,025 cm memperlihatkan bakteriluminisensi tidak memancarkan cahaya. Jadi konsentrasi bakteri sangat berperan dalam proses pemancaran cahaya pada bakteri luminisensi P. phosphoreum.Kata kunci: Isolasi bakteri, luminesensi, cumi-cumi (Loligo duvauceli)Bioluminescence refers to the visible light emission in liveng organisms that accompanies the oxidation of organic compounds (luciferins), mediated by an enzyme catalyst (luseferase). Result on the identification of bacteria live in the cuttlefish of L. duvauceli showed species of Photobacterium phosphoreum and the colony emits a blue lights. P. phosphoreum is the brightest bacteria in terms of emitting lights compared to other species of luminous bacteria. The total bacterial count gives 4.6 109 CFU/ml with diameter of colony of 0.075 cm which able to emit lights, while lower concentration at 2.0 104 CFU/ml and colony diameter of 0.025 cm give no emission of light. In respect to this, it suggest that concentration of luminous bacteria has role in theprocess of light emission by P. phosphoreum.Key words: Bacteria isolation, bioluminescence bacteria determination, squid (Loligo duvauceli)

Co-Authors Adi Santoso Adityo Harmawan Adityo Harmawan Agung Sudaryono Agus Hartoko Agus Sabdono Agus Trianto Agus Trianto Aiyen Tjoa Alfi Satriadi Alfin Anggraeni Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridho Ali Ridho Ali Ridho Ali Ridlo Ambariyanto Ambariyanto Amelia C. Anggelina Angelina Ferawaty Siregar Angelina Ferawaty Siregar Angelina, Amelia Cahya Anggelina, Amelia Cahya Anggi Setiabudi Aninditia Sabdaningsih Aninditia Sabdaningsih Anita Dwi Kartika Annisa Rhamadany Anuwat Nateewathana Ari Setyati , Wilis Arief Dwi Kurniawan Ariyanto, Dafit Ariyanto Avigail, Yolanda Bunjamin Dharma Chrisna Adhi Suryono Dafit Ariyanto Dafit Ariyanto Dewi, Lutfianna Fatma Diah Ayu Mustofa Diah Tri OktaviyantI Dinda Richa Kumarahaqi Dinny Anjang Sari Dolorosa, Roger G. Dony Bayu Putra Pamungkas Dwi Haryanti, Dwi Dwicahyo Setiyo Wibowo Edi Setiyono Eko Nurcahya Dewi Endah Sari Endang Sri Susilo Endang Sri Susilo Ervia Yudiati Ervia Yudiati Esti Rudiana Eunike Noviana Pranoto Evi Lutfiyani Fadhliyah Idris Fahmi Rizal Gaol, Josua Gabriel Lumban Girsang, Putri Hutari Gunawan Widi Santosa Gunawan Widi Santosa Hadi Endrawati Haeruddin Haeruddin Haeruddin Haeruddin Hanif, Marwa Irfan Haydar, Haydar Ali Yahya Hendrayana Hendrayana Hilda Ayu Aprilia Hilda Ayu Aprilia Idris, Riyada Iin Putriyani Indrawan , Ahmad Faras Irwani Irwani Ita Riniatsih Jelita Rahma Hidayati Kartika Dewi Koichiro Awai Lumban Gaol, Josua Gabriel Lutfianna Fatma Dewi Ma'ruf, Widodo Farid Masnah Jumiati Maulina, Dinda Rizky Ayu Mijil Ciptaning Dananjoyo Monika, Rika Muhammad Fatoni Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Muhammad Zainuddin Mujiyanto Mujiyanto Mukti K. Diana Putri Mukti K. Diana Putri Mustofa, Diah Ayu Nerva Sembiring Ni'mah, Ulin Nur Taufiq Nur Taufiq S.P.J. Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Nuraini , Ria Azizah Tri Nurul Fitrah Hasanah Nurul Fitrah Hasanah Nuskiya, Amila Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Pasaribu, Roberto Patar Person Pesona Renta Pranoto, Aris Kabul Putri Hutari Girsang Putri, Dear Berliana Putut Har Riyadi Retno Hartati Rhamadany, Annisa Ria Azizah Ria Azizah T.N. Ria Azizah Tri Nuraeni Ria Azizah Tri Nuraini Ribka Anindita Br Perangin-angin Richardus Mahatmada Indrajati Rignolda Djamaludin Rika Monika Rini Pramesti Rini Widiyadmi Rini Widyadmi Rudhi Pribadi Ryandha Idris Sakti Imam Muchlissin Salsabilla, Sauwa Khusna Sari Budi Moria Sarif, Fina Saffuteri Setiyorini, Alin Setyati , Wilis Ari Sibero, Mada T Siti Aisyah Siti Rudiyanti Sri Lintang Artono Sri Redjeki Sri Redjeki Sri Sejati Sri Yulina Wulandari Stevy Imelda Murniati Wodi Subagiyo Subagiyo Suryani, Askiya Intan Tatas H.P. Brotosudarmo Tedi Septiadi Tri Adi Nugroho Ucu Yanu Arbi Ulfah Rahmayani Ulin Ni'mah Vicencius Hendra Adhari Vincent Theodorus Siringoringo Vivi Endar Herawati Wahyudi, Dimas Waluyo, Anasri Widayat, Barra Muzaffar Widianingsih Widianingsih Wijaya, Patria Ami Wijayanti, Diah P. Wilis Ari Setyati Wilis Ari Setyati Yolanda Avigail Zaenal Arifin Zaenal Arifin Zaneb M. Ben Mansur

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