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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Natur Indonesia Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Journal of Aquatropica Asia Jurnal Kelautan Tropis Panrita Abdi - Jurnal Pengabdian pada Masyarakat JFMR (Journal of Fisheries and Marine Research) JRST (Jurnal Riset Sains dan Teknologi) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pengelolaan Perairan Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Natural Pigments Aquacultura Indonesiana Journal of Tropical Marine Science Indonesian Journal of Mollusk JURNAL BIOLOGI PAPUA Journal of Fisheries & Marine Jurnal Ilmu Kelautan Lesser Sunda

Delianis Pringgenies

Jurusan Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro Jl. H. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275

Author-ID : 347619

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Social Sciences Other

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Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus Delianis Pringgenies; Endah Sari; Widianingsih Widianingsih; Anuwat Nateewathana
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 3 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.3.182-188

Namanereis sp. belongs to class Polychaeta, a group of main benthic community in marine ecosystems. The purpose of this study to determine the potential of bioactive compounds from Namanereis sp. extract as an antibacterial agent and to determine its extract content through Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Samples of Namaneries sp. were collected from Demak Waters, Central of Java. This research used experimental laboratory method. Samples were extracted with non-polar (N-Hexane), semi-polar (Ethyl acetate) and polar (Methanol) solutions. Different concentrations of extraction, i.e. 5, 10, 20, 40, 80, and 100 µg.disk-1. then were tested against pathogenic bacteria Escherichia coli and Staphylococcus aureus. The extract content analysis was obtained using the GC-MS method. The results showed that Namanereis sp. extract had a potential as an antibacterial agent. The highest inhibition zone was seen in the fraction with ethyl acetate solvent at a concentration of 100%, namely 13±3.05 mm in E. coli and 14±3.04 mm in S. aureus, which then was analysed with GC-MS. Its results found 20 bioactive compounds in the extract. The five most dominant compounds determined by the highest peak were Dihydrojasmone (12.31%), Hexanoic acid (11.49%), Cholesterol (7.66%), Cyclohexanol (7.53%) and Isopropyl Palmitate (7.52%). The research concluded that Namanereis sp. possessed antibacterial potency and contained several bioactive compounds.

Bakteri Simbion Gastropoda Pleuroploca trapesium Dari Perairan Ternate, Sebagai Alternatif Antibakteri MDR (Bacterial Symbiont Gastropoda Pleuroploca trapezium from Ternate, as Alternative Antibacterial MDR) Delianis Pringgenies; Person Pesona Renta
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 1 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Bakteri yang resisten terhadap beberapa jenis antibakteri ini dikenal dengan bakteri multi drug resistant (MDR).Untuk mengatasi permasalahan tersebut, perlu dilakukan pencarian senyawa antibiotik baru yang lebih efektif dan efisien dalam mengatasi permasalahan bakteri MDR. Penelitian bertujuan untuk mengetahui potensi bakteri yang bersimbiosis dengan gastropoda Pleuroploca trapezium sebagai sumber antibakteri MDR. Sampel Moluska dikoleksi dari perairan Ternate, Maluku. Tahapan penelitian meliputi isolasi bakteri, skrining bakteri simbion yang potensi sebagai anti bakteri MDR, uji antibakteri, isolasi bakteri patogen klinis MDR; uji sensitivitas anti-bakteri, ekstraksi, amplifikasi dan sekuensing DNA. Hasil 16S urutan r-DNA dianalisis dan diedit menggunakan program Genetix dan diikuti dengan analisis urutan 16S rDNA. Hasil menunjukkan bahwa terdapat 19 isolat bakteri dengan 5 bakteri aktif yang berasosiasi dengan Pleuroploca trapezium. Berdasarkan besarnya zona hambat yang dibentuk dan konsistensi munculnya zona hambatan, isolat terbaik adalah TPT 4.7. Isolat ini memiliki hubungan yang dekat dengan Paracoccus sp. MBIC4019 dengan homologi sebesar 95% yang menunjukkan kekerabatan ditingkat genus. Hasil penelitian ini memberikan harapan adanya potensi besar sebagai bahan antibakteri baru. Kata kunci: antibakteri, simbion, Pleuroploca trapezium, multi drugs resistantThe bacteria resistant to some antibiotics are known as multi drug resistant (MDR). To overcome the problem, it is needed to search for a new antibiotic compounds more effectively and efficiently. This study aims to identify potential from symbionts of Pleuroploca trapezium as a source of antibacteria MDR and identifying the bacteria that were active against the MDR. Samples were collected from Ternate, Maluku. Isolation of symbiotic bacteria, screening for bacteria which producing secondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria of MDR. Conducting anti-bacterial sensitivity test, sensitivity test for antibacterial, DNA exctraction, DNA amplification based on PCR method, DNA sequencing. Result of 16S r-DNA sequence was then analyzed and edited using GENETYX program and followed by 16S rDNA sequence analysis. Screening of bacteria associated with P. trapezium resulted in 19 isolates with 5 active bacteria. Based on the size of the zone forming and the consistency of zone, so the best isolate is TPT 4.7. The identification shows that TPT 4.7 has a close relationship with the Paracoccus sp. MBIC4019 with homologi of 95%, which shows the relationship at the genus level. Its suggest that these results are very promising as a new antibacterial material. Keywords: antibacterial, symbiotic bacteria, Pleuroploca trapezium, multi drugs resistant

Penempelan Larva Teripang Putih (Holothuria scabra) pada Substrat yang Berbeda Retno Hartati; Widianingsih Widianingsih; Delianis Pringgenies; Nur Taufiq; Ali Djunaedi; Sari Budi Moria
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 3 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Metamorfosis pada larva teripang diikuti dengan perubahan tingkah lakunya. Stadia auricularia bersifat planktonic sedangkan stadia akhir doliolaria dan awal pentactula bersifat bentik dan memerlukan substrat untuk menempel. Perubahan ini merupakan saat kritis dalam pembenihan teripang sehingga substrat yang sesuai perlu diberikan pada media pemeliharaan larva. Penelitian ini bertujuan untuk mengetahui kesuakaan penempelan larva teripang putih (H. scabra) pada substrat asbes, kasa plastik dan kaca. Penghitungan densitas larva yang menempel pada tiap substrat dilakukan sejak larva mulai menempel pada hari ke-25 hingga larva mencapai stadia juvenil-umur 35 hari. Hasil penelitian menunjukkan bahwa substrat memberikan pengaruh yang berbeda nyata (P>0.05) terhadap jumlah larva yang menempel. Larva lebih banyak menempel pada substrat asbes (rata-rata 425,28 individu.m2 dengan kelulushidupan 9,96 % tetapi substrat kaca paling sedikit ditempeli larva (85,18 individu.m2 dengan kelulushidupan 3,98%). Perbedaan jumlah larva yang menempel pada perlakuan substrat diduga berkaitan dengan kekasaran permukaan substrat.Kata kunci: larva teripang putih; penempelen; substrat The metamorphoses of seacucumber larvae indicate changing in their behaviour. Auricularia stage is planktonic but late doliolaria and early pentactula are benthic and need suitable substrate. It is a critical period in development and culture of seacucumber larvae since the biggest mortality happened during metamorphosis and settlement therefore the suitable substrata need to be provided. The aims of the experiment was to determine larvae's preference on substrate made of asbestos, plastic screen and glass. Density of larvae settled on the substare were counted on day 25 up to juvenile stage of 35 days old. The results showed that the treatment gave significant differences on larvae settlement on the substrate. The larvae found attached on asbestos was highest (425,28 ind.m2 and survival rate 9.96 %) and lowest number larvae settled on glass (85,18 ind.m2 and survival rate 3,98 %). It seemed due to difference roughness of surface.Keywords: larvae seacucurnber; settlement; substrats

Stok Karbon Pada Tegakan Vegetasi Mangrove Di Pulau Karimunjawa Ria Azizah Tri Nuraini; Delianis Pringgenies; Chrisna Adhi Suryono; Vicencius Hendra Adhari
Buletin Oseanografi Marina Vol 10, No 2 (2021): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v10i2.31616

Hutan Mangrove merupakan suatu ekosistem laut yang memiliki peran penting sebagai penyimpan karbon tertinggi di wilayah tropis. Fungsi mangrove ini dapat membantu dalam mengurangi emisi karbon dan pemanasan global. Pulau Nyamuk dan Pulau Parang merupakan vegtasi alami yang mengalami penurunan luasan mangrove yang diduga disebabkan oleh alih fungsi lahan, sehingga akan menyebabkan penurunan fungsi mangrove dalam menyerap CO2. Tujuan dilakukannya penelitian ini adalah menganalisis dan menduga total simpanan karbon dan serapan CO2 pada tegakan mangrove di Pulau Nyamuk dan Pulau Parang. Manfaat dilakukanya penelitian ini adalah memberikan informasi mengenai manfaat mangrove sebagai penyerap karbon. Pengambilan data yang dilakukan dalam penelitian ini menggunakan purposive sampling method, dimana setiap lokasi memiliki 3 stasiun (Pulau Nyamuk; Sareh Besar, Sareh Kecil, Ujung; Pulau Parang: Batu Merah, Plawangan, Batu Hitam). Setiap stasiun dibagi menjadi 3 plot untuk dilakukan pengambilan data diameter batang mangrove. Data diameter batang mangrove digunakan untuk menghitung nilai biomasssa tegakan dengan menggunakan rumus allometrik dalam menduga simpanan karbon pada tegakan mangrove suatu area. Hasil penelitian menunjukan bahwa simpanan total karbon pada tegakan mangrove di Pulau Nyamuk memiliki nilai 1.176,48 ton/ha, yang dibagi sebagai berikut: Stsiun Sareh Kecil: 383,16 ton/ha, Stasiun Sareh Besar: 419,51 ton/ha, dan Stasiun Ujung: 373,81 ton/ha, sedangkan di Pulau Parang memiliki nilai 2009.031 ton/ha, yang dibagi sebagai berikut: Stasiun Batu Merah 767,672 ton/ha, Stasiun Batu Hitam 654,444 ton/ha, dan Stasiun Plawangan 586,915. Mangrove Forest is a marine ecosystem that has an important role as the highest carbon storage in the tropics. This mangrove function can help in reducing carbon emissions and global warming. Nyamuk Island and Parang Island are natural vegetation which decrease mangrove area which is caused by land conversion function, so it will cause mangrove function to absorb CO2. The purpose of this research is to analyze and estimate total carbon storage and CO2 uptake on mangrove stands in Nyamuk Island and Parang Island. The benefit of this research is to provide information about the benefits of mangroves as carbon sinks. The data collected in this research using purposive sampling methods, where each location has 3 stations (Nyamuk Island: Sareh Besar, Sareh Kecil, Ujung; Parang Island: Batu Merah, Plawangan, Batu Hitam). Each station is divided into 3 plots for data collection of mangrove stems diameter. The mangrove stem diameter data were used to calculate the stand biomass value by using allometric formula in estimating carbon stock in the mangrove stand of an areas. The results of research, that total carbon deposits in mangrove stands in Nyamuk Island had a value of 1,176.48 tons/ha, divided as follows: Sareh Kecil Station: 383.16 tons / ha, Sareh Besar Station: 419.51 tons / ha, and Ujung Station: 373,81 ton / ha, while in Parang Island has value 2009.031 ton / ha, which is divided as follows: Batu Merah Station 767,672 ton / ha, Batu Hitam Station 654,444 ton / ha, and Plawangan Station 586,915.

Kontribusi Lamun Enhalus acoroides Terhadap Kelimpahan Perifiton Di Perairan Legon Boyo, Karimunjawa Hendrayana Hendrayana; Ambariyanto Ambariyanto; Delianis Pringgenies; Mujiyanto Mujiyanto
Buletin Oseanografi Marina Vol 9, No 2 (2020): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v9i2.27346

Kelimpahan perifiton dipengaruhi oleh kerapatan lamun karena pada semakin tinggi kerapatan lamun maka kecepatan arus akan berkurang sehingga dapat meningkatkan laju penempelan perifiton. Penelitian ini bertujuan untuk mengetahui hubungan kerapatan lamun Enhalus acoroides dengan kelimpahan perifiton di Perairan Legon Boyo, Karimunjawa Penelitian ini menggunakan metode deskriptif dan pengambilan sampel menggunakan metode purposive sampling. Penelitian dilakukan pada bulan Juni, September dan Desember Tahun 2012. Hasil penelitian menunjukkan bahwa perifiton yang ditemukan pada lamun Enhalus acoroides di Perairan legon sebanyak 10 jenis dan jenis Baciliarophyceae merupakan jenis perifiton paling banyak ditemukan. Kelimpahan terbesar terjadi di musim peralihan yaitu sebesar 2.146 sel/l. Keanekaragaman jenis perifiton di lamun E. acoroides memiliki nilai antara 0,26-1,43 (Keanekaragaman rendah-sedang), keseragaman dengan nilai 3,61-5,82 (keseragaman tinggi) dan dominansi dengan nilai 0,68-1,12 (dominansi sedang-tinggi). Hasil analisis regresi hubungan kerapatan lamun dengan kelimpahan perifiton sebesar 79,13% yang menunjukkan hubungan kuat. Dengan demikian kerapatan lamun berpengaruh terhadap kelimpahan perifiton pada lamun. Periphyton abudance is influence by Seagrass densit. The aim of the research was to determaine Enhalus acoroides seagrass density and periphyton abudance correlation in Legon Boyo, Karimunjawa waters. Descriptive method and purposive sampling used in the research. The result show perphyton found as 10 kinds ang Baciliarophyceae clases are most of periphyton found. The most abundant periphyton in the transitional season is 2,146 cells/l. Periphyton species diversity in E.acoroides seagrass has a value between 0.26-1.43 (low-moderate diversity), uniformity with a value of 3.61-5.82 (high uniformity) and dominance with a value of 0.68-1.12 (moderate-high dominance). Correlation relationship with periphyton abundance was 79.13%, which showed a strong relationship. This value show of seagrass density influence periphyton abudance in seagrass.

Antioxidant Activities, Total Phenolic Compound And Pigment Contents of Tropical Sargassum sp. Extract, Macerated In Different Solvents Polarity Jelita Rahma Hidayati; Ervia Yudiati; Delianis Pringgenies; Zaenal Arifin; Diah Tri OktaviyantI
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Exposure of sunlight lead tropical Sargassum sp. to maintain their growth and moreover to bring up their secondary metabollite for life struggling. Sargassum sp. has bioactive compounds that has a potential antioxidant acitivity such as phenolic compounds as well as chlorophyll and carotenoids. This research was conducted to determine antioxidant activities, phenolic compound and pigmens of Sargassum sp. with different solvent that have different polarities. Sample was macerate with n-heksana, ethyl acetate, methanol and aquadest. All the parameters were done spectrophotometrically. IC50 was used to determine the antioxidant activity by antiradical scavenging activity using DPPH (515 nm). Total phenolic compound were tested by Folin-Ciocalteu solution and used gallic acid as standard (725 nm). The chlorophylls a content were measured at wavelength 662 nm and 645 nm and carotenoids were measured at wavelength 470 nm. The results showed best IC50 is achieved by aquadest extract (72.95 ±0.22 ppm). The highest Total phenolic compound is achieved by ethyl acetat extract (120.29 ±0,404 mg GAE/g sample). The highest chlorophyll a content is achieved by ethyl acetat extract (18.23 ± 0,049 mg/g sample) and the highest carotenoid content is achieved by ethyl acetat extract extract (60.65 ±0,008 µmol/g sample). It can be concluded that aquadest extract can be categorized as the strong antioxidant and antiradical activity, ethyl acetat as a medium antioxidant activity. The simple and save methods of aquadest extract promising that Sargassum sp. frorn Indonesia is a good candidate compoud for nutraceutical and cosmeceutical approach.

Aktivitas Antibakteri Ekstrak Jeruju Acanthus ilicifolius terhadap Bakteri Multi Drug Resistant Delianis Pringgenies; Wilis Ari Setyati; Dwicahyo Setiyo Wibowo; Ali Djunaedi
Jurnal Kelautan Tropis Vol 23, No 2 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i2.5398

This study aims to determine the antibacterial activity of the Jeruju fraction extract (roots, stems and leaves) against Multi Drug Resistant (MDR) bacteria. Extraction uses the solid-liquid extraction method. Fractionation was done by Thin Layer Chromatography (TLC) and Open Column Chromatography (OCC). The antibacterial activity was tested by the diffusion method for different concentrations. The test bacteria were MDR bacteria: Klebsiella sp, Coagulant negative stapylococi (CNS), Enterobacter 5, Enterobacter 10, E. Coli and Pseudomonas sp. The activity test results showed the crude extract of the root with ethyl acetate solvent had the highest antibacterial activity. The results of the Open Column Chromatography activity test showed that the fraction I, II and III of the root extracts had antibacterial activity against the test bacteria. Fraction I was active against Enterobacter 5 with inhibition zones of 10.0 ± 0.34 mm. Fraction II was active against Coagulant negative stapylococi with inhibition zone diameters of 11.80 ± 0.16 mm. Fraction III has the highest antibacterial activity against Enterobacter 10, Klebsiella sp, Pseudomonas sp. danE. Coli successively produce inhibition zones (13.98mm ± 0.58), (13.22mm ± 0.50), (13.15mm ± 1.15) and (13.10mm ± 0.04) and the highest in and Results the study concluded that root extracts had the highest antibacterial bioactivity compounds compared to stem and leaf extract samples. Furthermore, the MDR antibacterial activity test showed that the sample III fraction had the best inhibitory zone on the best bacteria as an anti-bacterial MDR: Klebsiella sp, Enterobacter 10, E. Coli and Pseudomonas sp Penelitian ini bertujuan mengetahui aktivitas antibakteri fraksi ekstrak Jeruju, (akar, batang dan daun) terhadap bakteri Multi Drug Resistant (MDR). Ekstraksi menggunakan metode ekstraksi padat-cair. Fraksinasi dilakukan dengan Kromatografi Lapis Tipis (KLT) dan Kromatografi Kolom Terbuka (KKT). Uji aktivitas antibakteri dengan metode difusi agar konsentrasi yang berbeda. Bakteri uji adalah bakteri MDR: Klebsiella sp, Coagulant negative stapylococi (CNS), Enterobacter 5, Enterobacter 10, E. Coli dan Pseudomonas sp. Hasil uji aktifitas antibakteri menunjukan ekstrak kasar akar dengan pelarut etil asetat memiliki aktifitas antibakteri tertinggi pada semua bakteri uji. Hasil uji aktivitas fraksi Kromatografi Kolom Terbuka memperlihatkan fraksi I, II dan III ekstrak akar memiliki aktivitas antibakteri terhadap bakteri uji. Fraksi I aktif terhadap Enterobacter 5 dengan zona hambat 10,0±0,34 mm. Fraksi II aktif terhadap Coagulant negative stapylococi dengan diameter zona hambat 11,80±0,16 mm. Fraksi III memiliki aktivitas antibakteri yang paling tinggi terhadap Enterobacter 10, Klebsiella sp, Pseudomonas sp. dan E. Coli. berturut – turut menghasilkan zona hambat (13,98mm ±0,58), (13,22mm ±0,50), (13,15mm ±1,15) dan (13,10mm ±0,04) serta tertinggi pada dan Hasil penelitian disimpulkan bahwa ekstrak akar memiliki senyawa bioaktifitas antibakteri tertinggi dibandingkan sampel ekstrak batang dan daun. Selanjutnya uji aktifitas antibakteri MDR memperlihatkan bahwa sampel fraksi III memiliki zona hambat terbaik pada bakteri terbaik sebagai anti bakteri MDR: Klebsiella sp, Enterobacter 10, E. Coli dan Pseudomonas sp

Antioxidant activity of Alginate Oligosaccharides (AOS) from Sargassum sp. for Improving the Cutaneous Wound Enclosure in Zebrafish (Danio rerio) Jelita Rahma Hidayati; Ervia Yudiati; Delianis Pringgenies; Zaneb M. Ben Mansur; Fadhliyah Idris
Jurnal Kelautan Tropis Vol 24, No 3 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i3.12362

Sargassum sp. classified as brown seaweed which is known as an alginophyte (alginate producer). Alginate has undergone a depolymerization process called alginate oligosaccharides (AOS) and has been shown to have antioxidant activities to increase wound tissue recovery. This study aimed to determine the antioxidant activity of Alginate oligosaccharides (AOS) and their ability to improve the cutaneous wound enclosure in Zebrafish (Danio rerio). The IC50 value was used to calculate the ability of extract to inhibit free radicals using DPPH (516 nm). Zebrafish were immersed 12 hours before the injury and shortly after injury with a two factorial design, i.e., alginate concentration and immersion time. Zebrafish were immersed for 1 hour, 3 hours, and 5 hours with serial concentration of 200 ppm, 400 ppm, and 600 ppm, respectively. Morphological observations were carried out at the the first day, fourth day, tenth day, and twenty-first-day post wounding. The results of this study showed that alginate from Sargassum sp. has a yield of 40.5 ± 1.125% with a purity level of 89.95%. Based on antioxidant activity, alginate is categorized as moderate (178,377 ppm) and evidently has the ability to increase wound recovery compared to control. It has indicated by the formation of the wound enclosure.

Determinasi Bakteri Simbion Luminesensi Cumi Loligo edulis Serta Analisis Potensinya Sebagai Anti Bakteri Delianis Pringgenies; Dinny Anjang Sari; Ria Azizah T.N.; Ervia Yudiati; Endang Sri Susilo; Alfi Satriadi
Jurnal Kelautan Tropis Vol 20, No 2 (2017): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The aim of this research is to determine luminous bacteria that symbiont with the light organ of the squid Loligo edulis and also to find out the potency of simbiont bacteria in light organ of the squid L. edulis. The squid L. edulis were collected from Teluk Awur Waters, Jepara. Stages of this research began with the isolation of bacteria, bacteria screening for anti bacteria, antibacterial activity test and and determination of bacterial species of the isolate. The result of determination showed that luminous bacteria which symbiont with the light organ of the squid L. edulis is come from species of Photobacterium phosphoreum. The result showed that luminous bacteria which symbiont with the light organ of the squid L. edulis have potency as antibacterial compound. Bacteria P. phosphoreum which symbiont in light organ of the squid L. edulis could inhibit the growth of Vibrio harveyi (diameter of resistance zone had range from 8,30-8,87 mm), Escherichia coli (diameter of resistance zone had range from 7,84-8,45 mm), Staphylococcus aureus (diameter of resistance zone had range from 8,39-9,09 mm) and Bacillus sp (diameter of resistance zone had range from 8,27-9,01). Tujuan dari penelitian ini adalah untuk mendeterminasi bakteri simbion yang bercahaya yang pada organ ringan dan mengetahui potensi bakteri simbion pada organ ringan dari cumi cumi Loligo edulis. Cumi L. edulis dikoleksi dari perairan Teluk Awur, Jepara. Tahapan penelitian meliputi: isolasi bakteri, skrining bakteri, uji aktivitas antibakteri dan dan identifikasi spesies bakteri isolat. Hasil penelitian memperlihatkan bahwa bakteri luminensensi yang bersimbiosis dengan organ cahaya cumi L. edulis adalah bakteri jenis Photobacterium phosphoreum. Bakteri luminesensi yang menempel pada organ cahaya cumi L. edulis memiliki potensi sebagai senyawa antibakteri. Bakteri P. phosphoreum yang tergabung edulis dapat menghambat pertumbuhan bakteri pathogen Vibrio harveyi (diameter zona resistansi berkisar antara 8,30-8,87 mm), Escherichia coli (diameter zona resistansi berkisar antara 7,84-8,45 mm), Staphylococcus aureus (diameter zona resistansi berkisar antara 8,39-9,09 mm) dan Bacillus sp (diameter zona resistansi berkisar antara 8,27-9,01).

Efek Panjang Gelombang Terhadap Pertumbuhan Propagul Pada Kultur Jaringan Eucheuma cottonii Doty, 1885 (Rhodophyceae; Solieracea) Wilis Ari Setyati; Rini Pramesti; Delianis Pringgenies; Chrisna Adhi Suryono; Irwani Irwani; Muhammad Zainuddin
Jurnal Kelautan Tropis Vol 23, No 3 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i3.7075

The problem in cultivating Eucheuma cottonii is the procurement of seeds and techniques currently developed through tissue culture. The limiting factor in this technique is the use of optimal light for the growth of the seaweed. The aims of study was to optimize the wavelength of light on the growth of E cottonii propagules. The research method is laboratory experimental with the treatment of different wavelengths of light: red light wavelength (λ = 633.8 nm), green (λ = 515.8 nm), blue (λ = 455.7 nm), combined light on the lamp LED (λ = 456.6 nm, 515.8 nm and 632.9 nm), and fluorescent light in TL lamps (λ = 407 nm, 443 nm, 557 nm and 592 nm). The results showed that the wavelength had a significant effect (p ≤0.05) on the growth of E cottonii. The best treatment for blue light with absolute, relative and specific growth values of propagule weight of 155 ± 11.910 mg, 419 ± 70.849%, and 5.860 ± 0.501% / day. The absolute, relative and specific growth values for propagule diameter were 701 ± 123.1 mm, 63 ± 12% and 1.73 ± 0.27% / day. The percentage of branching growth and the branching index were 60.85 ± 9.16% and 27.77 ± 1.23. Blue light treatment is optimal radiation in the E. cottonii tissue culture Permasalahan dalam budidaya Eucheuma cottonii adalah pengadaan bibit dan teknik yang berkembang saat ini melalui kultur jaringan. Faktor pembatas dalam teknik ini adalah penggunaan cahaya yang optimal untuk pertumbuhan rumput laut. Penelitian bertujuan untuk melakukan optimasi panjang gelombang cahaya terhadap pertumbuhan propagul E cottonii. Metode penelitian secara experimental laboratoris dengan perlakuan perbedaan panjang gelombang cahaya : panjang gelombang lampu cahaya merah (λ = 633,8 nm), hijau (λ = 515,8 nm), biru (λ = 455,7 nm), cahaya gabungan pada lampu LED (λ = 456,6 nm, 515,8 nm dan 632,9 nm), dan cahaya flourescent pada lampu TL (λ = 407 nm, 443 nm, 557 nm dan 592 nm). Hasil penelitian menunjukkan bahwa panjang gelombang berpengaruh signifikan (p < 0,05) terhadap pertumbuhan E cottonii. Perlakuan terbaik pada cahaya biru dengan nilai pertumbuhan mutlak, relatif dan spesifik bobot propagul sebesar 155±11,910 mg, 419 ± 70,849 %, dan 5,860 ± 0,501 %/hari. Nilai pertumbuhan mutlak, relatif dan spesifik diameter propagul sebesar 701±123,1 mm, 63±12 % dan 1,73±0,27 %/hari. Persentase pertumbuhan percabangan dan indeks percabangan sebesar 60,85±9,16 % dan 27,77±1,23. Perlakuan sinar biru merupakan penyinaran optimal dalam kultur jaringan E. cottonii.

Co-Authors Adi Santoso Adityo Harmawan Adityo Harmawan Agung Sudaryono Agus Hartoko Agus Sabdono Agus Trianto Agus Trianto Aiyen Tjoa Alfi Satriadi Alfin Anggraeni Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridho Ali Ridho Ali Ridho Ali Ridlo Ambariyanto Ambariyanto Amelia C. Anggelina Angelina Ferawaty Siregar Angelina Ferawaty Siregar Angelina, Amelia Cahya Anggelina, Amelia Cahya Anggi Setiabudi Aninditia Sabdaningsih Aninditia Sabdaningsih Anita Dwi Kartika Annisa Rhamadany Anuwat Nateewathana Ari Setyati , Wilis Arief Dwi Kurniawan Ariyanto, Dafit Ariyanto Avigail, Yolanda Bunjamin Dharma Chrisna Adhi Suryono Dafit Ariyanto Dafit Ariyanto Dewi, Lutfianna Fatma Diah Ayu Mustofa Diah Tri OktaviyantI Dinda Richa Kumarahaqi Dinny Anjang Sari Dolorosa, Roger G. Dony Bayu Putra Pamungkas Dwi Haryanti, Dwi Dwicahyo Setiyo Wibowo Edi Setiyono Eko Nurcahya Dewi Endah Sari Endang Sri Susilo Endang Sri Susilo Ervia Yudiati Ervia Yudiati Esti Rudiana Eunike Noviana Pranoto Evi Lutfiyani Fadhliyah Idris Fahmi Rizal Gaol, Josua Gabriel Lumban Girsang, Putri Hutari Gunawan Widi Santosa Gunawan Widi Santosa Hadi Endrawati Haeruddin Haeruddin Haeruddin Haeruddin Hanif, Marwa Irfan Haydar, Haydar Ali Yahya Hendrayana Hendrayana Hilda Ayu Aprilia Hilda Ayu Aprilia Idris, Riyada Iin Putriyani Indrawan , Ahmad Faras Irwani Irwani Ita Riniatsih Jelita Rahma Hidayati Kartika Dewi Koichiro Awai Lumban Gaol, Josua Gabriel Lutfianna Fatma Dewi Ma'ruf, Widodo Farid Masnah Jumiati Maulina, Dinda Rizky Ayu Mijil Ciptaning Dananjoyo Monika, Rika Muhammad Fatoni Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Muhammad Zainuddin Mujiyanto Mujiyanto Mukti K. Diana Putri Mukti K. Diana Putri Mustofa, Diah Ayu Nerva Sembiring Ni'mah, Ulin Nur Taufiq Nur Taufiq S.P.J. Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Nuraini , Ria Azizah Tri Nurul Fitrah Hasanah Nurul Fitrah Hasanah Nuskiya, Amila Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Pasaribu, Roberto Patar Person Pesona Renta Pranoto, Aris Kabul Putri Hutari Girsang Putri, Dear Berliana Putut Har Riyadi Retno Hartati Rhamadany, Annisa Ria Azizah Ria Azizah T.N. Ria Azizah Tri Nuraeni Ria Azizah Tri Nuraini Ribka Anindita Br Perangin-angin Richardus Mahatmada Indrajati Rignolda Djamaludin Rika Monika Rini Pramesti Rini Widiyadmi Rini Widyadmi Rudhi Pribadi Ryandha Idris Sakti Imam Muchlissin Salsabilla, Sauwa Khusna Sari Budi Moria Sarif, Fina Saffuteri Setiyorini, Alin Setyati , Wilis Ari Sibero, Mada T Siti Aisyah Siti Rudiyanti Sri Lintang Artono Sri Redjeki Sri Redjeki Sri Sejati Sri Yulina Wulandari Stevy Imelda Murniati Wodi Subagiyo Subagiyo Suryani, Askiya Intan Tatas H.P. Brotosudarmo Tedi Septiadi Tri Adi Nugroho Ucu Yanu Arbi Ulfah Rahmayani Ulin Ni'mah Vicencius Hendra Adhari Vincent Theodorus Siringoringo Vivi Endar Herawati Wahyudi, Dimas Waluyo, Anasri Widayat, Barra Muzaffar Widianingsih Widianingsih Wijaya, Patria Ami Wijayanti, Diah P. Wilis Ari Setyati Wilis Ari Setyati Yolanda Avigail Zaenal Arifin Zaenal Arifin Zaneb M. Ben Mansur

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