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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Buletin Hama dan Penyakit Tumbuhan USU LAW JOURNAL BIOTROPIA - The Southeast Asian Journal of Tropical Biology Cropsaver : Journal of Plant Protection Jurnal Penelitian Hutan Tanaman Jurnal Hortikultura Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Planta Tropika Jurnal Perlindungan Tanaman Indonesia JURNAL BIOLOGI INDONESIA ZOO INDONESIA JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Journal of Applied Agricultural Science and Technology Biosaintifika: Journal of Biology & Biology Education Jurnal Entomologi Indonesia Buletin Penelitian Tanaman Rempah dan Obat Zoo Indonesia Agrotech Journal Jurnal Agrisa Andalasian International Journal of Entomology
PURNAMA HIDAYAT
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Kamper, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Veterinary Other

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Deteksi dan Pembedaan Praktis Fitoplasma dari Beberapa Tanaman Inang Menggunakan PCR-RFLP Mutaqin, Kikin; Hidayat, Purnama; Tjahjono, Budi; Kusumah, Yayi Munara; Suseno, Rusmilah
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.188-195

Abstract

Deteksi dan Pembedaan Praktis Fitoplasma dari Beberapa Tanaman Inang Menggunakan PCR-RFLP Fitoplasma adalah prokariota fitopatogenik dengan kisaran inang yang luas dan merupakan patogen yang perlu mendapatkan perhatian lebih banyak di Indonesia. Patogen ini relatif sulit untuk dideteksi dan diidentifikasi mengingat sifat biologinya yang rumit. Penelitian ini meliputi deteksi fitoplasma menggunakan teknik polymerase chain reaction (PCR) dengan primer P1/P7 dari tujuh tanaman bergejala, yaitu daun putih rumput Bermuda, kuning bambu, sapu kacang tanah, kedelai, kacang panjang dan kaktus, serta daun kecil ubi jalar. DNA gen 16S rRNA fitoplasma hasil amplifikasi PCR selanjutnya direaksikan dengan masing-masing enzim endonuklease restriksi AluI, RSaI dan MSeI untuk menghasilkan pola restriction fragment length polymorphism (RFLP). Tujuh tanaman sakit tersebut terkonfirmasi berasosiasi dengan fitoplasma yang ditunjukkan oleh produk PCR sebesar 1800 pb. Berdasarkan profil RFLP tiga enzim, fitoplasma dibedakan ke dalam dua kelompok, yaitu kelompok I (rumput Bermuda dan bambu) dan kelompok II (kacang tanah, kedelai, kacang panjang, kaktus dan ubi jalar). Fitoplasma kaktus merupakan sub-kelompok (galur) karena memiliki sedikit perbedaan fragmen dari profil RFLP dengan enzim MSeI.
Neraca kehidupan kutukebul Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) pada tanaman cabai dan gulma babadotan pada suhu 25 °C dan 29 °C Subagyo, Vani Nur Oktaviany; Hidayat, Purnama
Jurnal Entomologi Indonesia Vol 11 No 1 (2014): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.11.1.11

Abstract

Bemisia tabaci (Gennadius) is an important pest on horticultural crops that can also become a vector of Geminivirus that transmit the yellow curl disease. B. tabaci can also attack weeds that grow around the field such as Ageratum conyzoides or goatweed. The objective of this research was to study the life cycle, life time, fecundity, and reproduction rate of B. tabaci on chili pepper and goatweed. The experiment was conducted by observing the development of 50 eggs into adulthood. All 50 eggs were put on one plant and repeated 3 times. The plants were grown at temperatures 25 °C and 29 °C inside growth chamber (L : D = 12 : 12). The result of this experiments showed that at both temperatures of 25 °C and 29 °C, reproductive rate (R0), intrinsic rate of increase (r) and fecundity of B. tabaci were higher on goatweed than chili pepper. This implies that the existence of goatweed around chili pepper in the field can serve as a good alternative host for B. tabaci, hereby supporting the pest population even further.
Kutudaun (Hemiptera: Aphididae) pada gulma di sekitar lahan pertanian di Jawa Barat beserta kunci identifikasinya Maharani, Yani; Hidayat, Purnama; Rauf, Aunu; Maryana, Nina
Jurnal Entomologi Indonesia Vol 15 No 2 (2018): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.15.2.68

Abstract

Aphids (Hemiptera: Aphididae) are considered as one the most important pest in the world. Some species of aphids were reported as serious pests and plant virus vectors, especially on horticultural crops. Weeds in the agricultural area can serve as an alternative hosts for some aphids which are plant pests or plant virus vectors. The objective of this research was to identify aphid species of weeds in the agricultural production area in West Java and to provide the identification key. Aphids collection was done on the weeds in the agricultural fields in 9 districts of West Java. Aphids were collected from 13 species (5 families) of weeds. The identification of aphid was peformed based on the adult specimens. The research manased to come up with 12 species of aphid on weeds. Three of the 12 collected species were Epameibaphis frigidae (Oestlund), Metopolophium dirhodum (Walker), and Pseudaphis sijui (Eastop) has never been reported in West Java. Six species were known as vectors of plant viruses in agricultural crops. The most commonly found aphid species was Schizaphis graminum (Rondani) in the yard grass (Eleusine indica).
Keanekaragaman spesies kutukebul (Hemiptera: Aleyrodidae) pada tanaman hortikultura dengan ketinggian tempat berbeda di Jawa Barat Nurulalia, Lia; Buchori, Damayanti; Hidayat, Purnama
Jurnal Entomologi Indonesia Vol 15 No 3 (2018): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.15.3.143

Abstract

Whitefly (Hemiptera: Aleyrodidae) plays an important role as pest in many horticultural plants (fruits, vegetables, and ornamental plants). The aim of this research was to study whiteflies diversity in horticultural plants on different altitude. Whitefly samples were collected from its host plants in five areas of West Java Bogor, Sukabumi, Cianjur, Bandung, and Garut. The places were grouped into three altitude categories: lowland, midland, and highland. Number of whitefly species and individual were analysed using analysis of variance (ANOVA) with post-hoc Tukey (α = 0.05), while the correlation between altitude and whiteflies species number described in linear regression. Species diversity was measured by two types of index (Shannon and Simpson). The highest number of whitefly individual was found in vegetables (121.80). However, the highest number of whitefly species was found in fruits (2.44 species). According to the altitude, the highest number of whitefly species was found in the lowland (4.67 species), whereas the lowest was found in the highland (1.58 species). The abundance of whiteflies population increases with increasing of altitude, whereas the number of species decreases with increasing of altitude. The highest number of whitefly species diversity was found in the lowland (H' = 2.08), meanwhile the lowest was in the highland (H' = 0.38). By contrast, according to the Simpson index, there was species domination of whitefly in the Highland (D = 0.54) by species Aleurodicus dugesii Cockerell
Tipe penggunaan lahan memengaruhi keanekaragaman dan komposisi hymenopteran parasitoid di Jambi Tawakkal, Muhammad Iqbal; Rizali, Akhmad; Larasati, Anik; Sari, Adha; Hidayat, Purnama; Buchori, Damayanti
Jurnal Entomologi Indonesia Vol 16 No 3 (2019): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.16.3.151

Abstract

Hymenoptera is one of the four largest insect orders in the world, one of which is its role as a natural enemy (parasitoids, predators). Land conversion can affect the diversity and composition of the parasitoid Hymenoptera and its ecological role. This study aims to examine the effect of land use types on the diversity and composition of hymenopterans parasitoid in Jambi. The study was conducted on various types of land use in the Harapan Forest landscape and Bukit Duabelas National Park (TNBD), Jambi Province including forests, rubber forests, oil palm plantations, and rubber plantations. Insect sampling was conducted from March to September 2013, using fogging techniques. A total of 14,258 hymenopteran parasitoid individuals consisting of 30 families were obtained from all study sites. Encyrtidae, Braconidae, Aphelinidae, Eulophidae, Scelionidae, Ceraphronidae, and Platigasteridae families are found in high abundance. Oil palm land use types are found to have different parasitoid compositions compared to other land use types, while forest and rubber forest land use types have high species composition. The results of this study indicate that the type of land use affects the diversity and composition of hymenopteran parasitoid species.
Pengaruh cahaya artifisial di malam hari (artificial light at night-ALAN) terhadap serangga Mawan, Amanda; Nazarreta, Rizky; Kasmiatun; Istiaji, Bonjok; Hidayat, Purnama; Buchori, Damayanti
Jurnal Entomologi Indonesia Vol 19 No 3 (2022): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.19.3.255

Abstract

Insect populations have declined significantly over the last few decades. Anthropogenic factors such as deforestation, land-use change, climate change, and pesticides play a major role in insect population decline. In addition to those factors, insects also face challenges from air, noise, and light pollution derived from human activities. Light pollution in the form of artificial light at night (ALAN) is generally known to be one of the factors driving insect declines but it has rarely been studied in Indonesia. In this literature review, we collected empirical evidence from previous studies to provide a comprehensive report on the impact of ALAN on insects. Most studies demonstrate a decline in insect populations in areas with high ALAN intensity, such as urban areas. Furthermore, ALAN was reported to disrupt insect visual systems, which results in disturbances to predator avoidance and foraging movements. Until now, most work related to artificial light in Indonesia have focused on light as a method of controlling pests. Hence, studies related to artificial light as a pollutant are urgently needed to increase our understanding of the effects of ALAN on the survival of organisms, especially insects. Future research must seek suitable solutions to create environmentally and ecologically friendly conditions for various organisms, especially those susceptible to the negative effects of light, such as insects.
Bemisia tabaci (Hemiptera: Aleyrodidae): evaluation of leaf trichome density based resistance on several soybean varieties Murgianto, Fitrah; Hidayat, Purnama; Triwidodo, Hermanu
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.1239-14

Abstract

The whitefly Bemisia tabaci (Gennadius) is one of the important soybean pests which can reduce production up to 80 %. Soybean defense mechanism against B. tabaci is affected by the physical characteristics of the leaf surface, e.g. leaf thickness, density of trichomes, and sticky glandular trichomes. The objective of this research was to investigate B. tabaci population – trichome density relationship on ten soybean varieties. The experiment was carried out in Ngale Station, Ngawi District, East Java. The tested soybean varieties were Anjasmoro, Demas 1, Dena 1, Dering 1, Devon, Detam 3, Dewah, Gema, Grayak 1, and Wilis. The experiment was done by completely randomized design with three replications. Observation on the whitefly population was conducted weekly from 2 until 10 weeks after planting on 23 sample plants in each plot. The result showed that Devon was the variety with the highest population levels of B. tabaci, 10.89 (eggs), 14.48 (nymphs) individuals per leaf, respectively. The population of B. tabaci was affected by trichomes density. Devon was the highest density of trichomes (613.7 hairs per cm2). The density of trichomes and the number of whitefly eggs and nymphs relationship were significant (R2= 0,78 (egg) and 0.84 (nymph), n= 10, ?= 1 %, F Value= 0.00157, respectively).
PERKEMBANGAN TEKNOLOGI IDENTIFIKASI SERANGGA DARI ZAMAN KE ZAMAN Indriati, Gusti; Hidayat, Purnama
JURNAL AGRISA Vol 12 No 2 (2023): Jurnal Agrisa
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/agrisa.v12i2.14024

Abstract

Insect classification is carried out using various identification techniques, namely conventional and modern. Accurate identification techniques for insect species are an important key in determining effective pest control measures. Insect classification and insect identification methods are interrelated because proper classification is a necessary basis for effective identification methods. The development of insect classification based on time is divided into several stages, namely: Pre-Linnean Era, Linnean Era, Darwinian Era, and Hennigian Era. The traditional method of identifying pest insects is done manually to recognize and classify pest insects based on morphological, anatomical and other characteristics. Although this method tends to be slower and requires a high degree of manual skill, it remains an important basis for taxonomy. Rapid progress in the world of research requires increased quality and speed of time, thus requiring modern and up-to-date insect identification methods such as Next-Generation Squencing (NGS), DNA Barcoding, Computer Vision, Mobile Apps and Citizen Science, and Micro CT Scanning. Supporting the implementation of modern technology requires software development, specialized equipment, and careful validation to ensure accuracy and efficiency as well as human resources. A combination of advanced technology and traditional methods may be the most effective approach in insect taxonomy identification.
Molecular Evidence Points to Strong Resemblance in the Parasitoid Species of Rice and Cogongrass Gall Midges, Platygaster spp. (Hymenoptera: Platygasteridae) Hidayat, Purnama; Lestari, Yuliana Ayu; Nurulita, Sari
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.683-692

Abstract

The rice gall midge, Orseolia oryzae, and the cogongrass gall midge, O. javanica, cause gall formation on rice and cogongrass (alang-alang) (Imperata cylindrica). Two different species parasitize these two gall midges but closely related platygasterids, Platygaster oryzae on the rice gall midge and P. orseoliae on the cogongrass gall midge. Both the gall midges and their parasitoids are often found in the adjacent area, raising a question about the relationship between the two gall midges and their parasitoids. This research aims to study the molecular identity of the rice and cogongrass gall midges, along with their platygasterid parasitoids, based on partial sequences of the mtCOI gene. Samples were collected from rice and cogongrass in the adjacent area in Cianjur, West Java Province, and a rice field with no cogongrass in Bogor, West Java Province. Successful DNA amplification was achieved using universal primers for mtCOI. Nucleotide sequencing analysis revealed that the rice gall from Bogor and Cianjur shared 100% similarity and 93.2-99.3% similarity with the rice gall from other countries. Notably, the parasitoids P. oryzae collected from rice in Bogor and Cianjur shared 97.2% similarity with P. orseoliae collected from cogongrass in Cianjur. These findings suggest that the platygaster parasitoids associated with the rice gall and the cogongrass gall midges are identical, serving as potential natural enemies for both pests. This study represents the first molecular identification report of rice and cogongrass gall midges and their platygasterid parasitoids from Java Island, Indonesia.
Comparative Study of Integrated Pest Management and Farmer's Standard Practices for Controlling Chrysanthemum Thrips under Plastic House Hutapea, Dedi; Sartiami, Dewi; Dadang, Dadang; Hidayat, Purnama
AGRIVITA Journal of Agricultural Science Vol 46, No 1 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i1.4018

Abstract

Thrips is an important worldwide cause of severe damage to various host plants, especially chrysanthemums. Current management still relies on synthetic insecticides to control thrips. However, these pesticides harm the environment and promote thrips resistance. The study aimed to compare integrated pest management (IPM) and farmer’s standard practices to control Thrips parvispinus on two chrysanthemum varieties and calculate the cost-benefit ratio of both pest control practices. Two chrysanthemum cultivars were planted in plastic houses to compare IPM and farmer’s standard practices. The observed variables included thrips density, attack and damage rates, cut flower yield, and natural enemies abundance. Data were analyzed using ANOVA, and Tukey's HSD test identified significant differences at a 5% level. The results showed that the population of T. parvispinus nymphs was higher (78%) than adults. The highest adult thrips population emerges when chrysanthemums achieve the flowering phases. This study reveals that the control effects of IPM were not different from farmer’s standard practices. A holistic approach integrating several management strategies successfully controlled the thrips population while producing high-quality crops with minimal aesthetic damage. The IPM strategy against thrips showed a competitive cost and reduced synthetic insecticide applications without decreasing the quality and productivity of chrysanthemums.
Co-Authors Ady Daryanto Akhmad Rizali ALI NURMANSYAH ANDI KHAERUNI Anik Larasati Anik Larasati Araz Meilin Awang Maharijaya Azhar, Azru B T Rahardjo Bambang Suryobroto Bambang Tri Rahardjo Bandung Sahari Bintoro, Denny Bismar Nasution Bonjok Istiaji Botek, Muhammad Budi Tjahjono Ciptadi Achmad Yusup DADANG DADANG DAMAYANTI BUCHORI Dede Maryana DEWI SARTIAMI DJOKO PRIJONO Dodi Nandika Endang Sri Ratna Fitrah Murgianto GUSNAWATY HS, GUSNAWATY Gusti Indriati Harleni Harleni Hasballah Thaib Hazen Arrazie Kurniawan Hendrival Hendrival Hermanu Triwidodo Husni dan Zulfanazli Pudjianto Alfian Rusdy Hutapea, Dedi I Wayan Winasa Inez H. Slamet -Loedin Juliet M.E. Mamahit Kasmiatun Keisha Disa Putirama Kenneth Puliafico Kiki Fajrina Simamora Kikin H Mutaqin Kurniawan, Rachman Kusumah, Yayi Munara Lestari, Yuliana Ayu LILIK BUDIPRASETYO Loedin, Inez H. Slamet Lutfi Afifah Mahindra Dewi Nur Aisyah Manuwoto, Syafrida Manuwoto, Syafrida Marwoto . Marwoto . MAWAN, AMANDA MELTA R. FAHMI Muhamad Syukur Muhammad Basri Murgianto, Fitrah Murgianto, Fitrah N. Usyati Neo Endra Lelana nFN Sudiono Nia Kurniawaty Nia Kurniawaty, Nia NOOR AIDAWATI Noor Farikhah Haneda Nurheni Wijayanto Nurjanah Nurulalia, Lia Oemijati Rachmatsyah Permatasari, Gusthi Ayu Rachman Kurniawan Rachman Kurniawan RACHMAWATI RACHMAWATI Rahayu Mallarangeng, Rahayu Rahayuwati, Sat Rahmini, Rahmini RAUF, AUNU Revhida Puspa Anisa Rika Ludji Rizky Nazarreta, Rizky Rosyid Amrulloh RUSMILAH SUSENO Safitri, Betari Sandi Nugroho Sari Nurulita SARI, ADHA SAURIN HEM Schulze, Christian H. Schulze, Christian H. SEDYO HARTONO Septiantina Dyah Riendriasari Shahabuddin Saleh Siswandi Siswandi SITI HERLINDA SJAFRIDA MANUWOTO SJAFRIDA MANUWOTO Sobir Sobir Soemartono Sosromarsono Soerjono Hadi Sutjahyo Sopian Sopian SRI HENDRASTUTI HIDAYAT Sri Hendrastuti Hidayati Sriani Sujiprihati Subagyo, Vani Nur Oktaviany Sudiono . Sudiono Sudiono Surjono Hadi Sutjahyo Sutjahyo, Surjono Hadi SYAFRIDA MANUWOTO Syafrida Manuwoto Syair, Syair Syawaluddin Syawaluddin Tawakkal, Muhammad Iqbal Tengku Laila Kamaliah Tri Atmowidi Triyani Dumaria Ujang Wawan Darmawan Ulfah Juniarti Siregar Usyati, N. Utary Maharani Barus UTOMO KARTOSUWONDO Utomo Kartosuwondo Van Basten Tambunan Van Basten Tambunan Vani Nur Oktaviany Subagyo Wijayanto, Nurheni Willing Bagariang Woro A. Noerdjito, Woro A. Woro Anggraitoningsih Noerdjito YAHER WANDI Yani Maharani Yani Maharani, Yani Yendra Pratama Setyawan Yoga Yuniadi Yuliani Yuliani YULIARTI, NETTI