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Jusna Ahmad
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Gorontalo
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Journal : BIOVALENTIA: Biological Research Journal

 1 
A In Vitro Callus Induction from (Antidesma bunius L.) Leaves Adju, Fitria Y.Hs.; Ahmad, Jusna; pagalla, Devi Bunga; Kandowangko, Novri Youla; Husain, Indriati; Isima , Lindawaty
BIOVALENTIA: Biological Research Journal Vol. 11 No. 1 (2025)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.11.1.2025.451

Abstract

Antidesma bunius L. is a tree species that has ecological and economic benefits, and has many bioactivities. Propagation of this tree can be done in several ways, one of which is callus induction. Callus is a collection of undifferentiated cells that can develop into new plants with the help of ZPT. The purpose of this study was to determine the effect and optimum combination of ZPT 2,4-D and BAP on the induction of leaf callus of A. bunius L. The research design was a non-factorial Completely Randomised Design (CRD) with 4 treatments namely P0: MS without the addition of zpt; P1: MS + 1 ppm BAP + 3 ppm 2,4-D; P2: MS + 2 ppm BAP + 2 ppm 2,4-D; P3: MS + 3 ppm BAP + 1 ppm 2,4-D. Parameters observed included callus emergence time, percentage of callus explants, and callus morphology. The results showed that P2 is the best concentration for callus induction, by looking at the observed effect on callus emergence time of 6 HST, percentage of callus explants 56% and callus morphology which is white with crumb texture, which can develop into embryogenic callus
Co-Authors Abubakar S. Katili Abubakar Sidik Katili Abubakar Sidik Katili Adam Suduri Adilah Nidaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad, Aisyah Akbar, Muh Nur Al-Hamid, Nurjihan Nissa Amir, Fadila Amir Amu, Verawaty Andi Pratiwi Angry Pratama Solihin Ani M. Hasan Ani M. Hasan Anis Safitri Anita Sabara Aryati Abdul Bano, Nurhayati S. Budy Santoso Butungale, Said Chairunnisa J Lamangantjo Chairunnisah J Lamangantjo Chairunnsah J. Lamangantjo Dahniar Dahniar Devi Bunga Pagalla Devi Bunga Pagalla Dewi Wahyuni K. Baderan Djafar, Fitri Yani Djafar, Nazwa H. Djuna Lamondo Dunggio, Fadlia Elya Nusantari Evita Moni Damayanti Eyato, Rika Fatmariani Abdul Wahab Febriyanti Febriyanti , Febriyanti Febriyanti Febriyanti Febriyanti Febriyanti febriyanti febriyanti Febriyanti, Febriyanti Fitria H.Ys. Adju Frida Maryati Yusuf Gogou, Elsi Gonibala, Alfikry Gubali, Hayati Ningsih H. Husain , Ilyas HASAN, ANI M. Hayatiningsih Gubali Herinda Mardin Herlina Jusuf Hilimi, Delvita Septianingsi Husain, Ilyas H. Ibrahim, Nurain Ibrahim, Rawiyanti Ilyas Husain Ilyas Husain Inaku, Irhamna Indriati Husain Intan Permatasari Irhamna Inaku Ishak, Sri Layla Putri Isima , Lindawaty Kadita P. Latif Kandowangko, Novri Youla Kirsten Caroline Donsi ladoma, wafiq aziza m. Lamangantjo, Chairunnisa J. Lamangida, Novi Safitri Lamante, Nazwa S. Lilan Dama Lindawaty Isima M, Maesarah Mahluk, Linda K. Mamu , Hartono D Mantali, Magfirah F. Margaretha Solang Marini Susanti Hamidun Masra Latjompoh Masrid Pikoli Mega Anastasya Diska Mokoginta Mega Rosalita Ekaputri Koni Menanti, Menanti Mestin Abdullah Miftahul F. Adudu Miftahul Fikriyah Adudu Moh G. H. Sahada Moh. Gifari Hi Sahada Mohamad Ikbal Riski Danial Mustamin Ibrahim Mustaqimah, Nur Najmah, Najmah Netty Ino Ischak Nina Tanjung Api Novri Youla Kandowangko Nurain Ain Djafar Nurain Djafar Nurdin, St. Surya Indah Nurma Rosalia Nurul Fajryani Usman Pagala, Devi Bunga Pagalla , Devi Bunga Pakaya, Melisa Patta Sija Podungge, Sandra Putri, Restiyani Widia Ramlah Ramlah Rulan U. Mustapa S. Ahmad, Sintia Salihun, Agil Saliko, Abdul Mazid Sari, Wahyuningsih Ratna Sela Restia Tangahu Sidik Katili , Abubakar Sija, Patta Sri Anggriyanti Putri Bohoka Sri Manovita Pateda Sukrin Ismail Syam S. Kumaji Tanaiyo, Sri Nuning B. Tandililing, Indayani Thalib, Moh. Randy Putra Pratama Tri Nugroho, Bagus Triska Wahyuni Dawit Uno, Wirnangsi Din. Vivien Novarina Kasim Walangadi, Farhana Rahmatia Widya Rahmawaty Saman Winda Minarti Jusuf Winda Minarti Jusuf Wirnangsi D. Uno Wirnangsi D. Uno Wirnangsih D. Uno Yasin, Indri Afriyani Yuliana Retnowati Yusuf, Septia Zuliyanto Zakaria