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Journal : Inventa: Journal of Science, Technology, and Innovation

Antagonisme Trichoderma sp. terhadap Colletotrichum capsici: Uji In Vitro sebagai Strategi Pengendalian Hayati Penyakit Antraknosa pada Cabai Rawit Triska Wahyuni Dawit; Ani M. Hasan; Jusna Ahmad; Yuliana Retnowati; Febriyanti, Febriyanti
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/dvr6m616

Abstract

Anthracnose disease is a plant disease caused by the fungus Colletotrichum capsici, the disease is one of the problems in the cultivation of cayenne pepper (Capsicum frutescens). This study aims to test the antagonistic ability of Trichoderma sp. against the fungus Colletotrichum capsici and observe the morphological changes in Colletotrichum capsici colonies exposed to Trichoderma sp in vitro on cayenne pepper plants Capsicum frutescens. The test method was carried out using the dual culture method to determine the percentage of inhibition by studying the interaction between Trichoderma sp. and Colletotrichum capsici colonies on Potato Dextrose Agar culture media. The results showed that Trichoderma sp. fungi can compete for space and resources against Colletotrichum capsici fungi macroscopically by controlling space and resources, and microscopically by producing antibiotics (antimicrobials) that can inhibit the growth of Colletotrichum capsici. The urgency of this research is the need for safe and environmentally friendly disease control efforts. One method that has been widely developed is by utilizing biological agents, such as Trichoderma sp. which is able to inhibit the growth of the pathogen Colletotrichum capsici.
Induksi Kalus dari Antera Pisang Goroho (Musa acuminata L.) dengan Menggunakan 2,4-D dan BAP Risna A. Onu; Jusna Ahmad; Febriyanti, Febriyanti; Novri Youla Kandowangko; Devi Bunga Pagalla; Indriati Husain
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/8t6ph238

Abstract

Callus induction is a critical stage in anther culture for supporting the propagation and genetic improvement of local banana cultivars. This study evaluated the effect of different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction from goroho banana (Musa acuminata L.) anthers. Observations were conducted for 30 days after induction, focusing on callus emergence time and morphological characteristics, including color and texture. The results demonstrated distinct responses among treatments. The combination of 1.5 ml 2,4-D and 1.5 ml BAP produced the fastest callus initiation with an average of 13.17 days after induction and generated friable callus with a pale yellow coloration, indicating high cellular activity. Lower growth regulator concentrations delayed callus formation, while higher concentrations promoted color changes associated with early tissue differentiation. No callus formation was observed in the absence of exogenous growth regulators. These findings highlight the importance of balanced auxin and cytokinin concentrations in regulating anther-derived callus induction and provide a physiological basis for further development of goroho banana anther culture systems.
Co-Authors Abubakar S. Katili Abubakar Sidik Kaili Abubakar Sidik Katili Abubakar Sidik Katili Adam Suduri Adilah Nidaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad, Aisyah Akbar, Muh Nur Al-Hamid, Nurjihan Nissa Amir, Fadila Amir Amu, Verawaty Andi Pratiwi Angry Pratama Solihin Ani M. Hasan Ani M. Hasan Anis Safitri Anita Sabara Aryati Abdul Bano, Nurhayati S. Budy Santoso Butungale, Said Chairunnisa J Lamangantjo Chairunnisah J Lamangantjo Chairunnsah J. Lamangantjo Dahniar Dahniar Devi Bunga Pagalla Devi Bunga Pagalla Devi Bunga Pagalla Dewi Wahyuni K. Baderan Djafar, Fitri Yani Djafar, Nazwa H. Djuna Lamondo Elya Nusantari Evita Moni Damayanti Eyato, Rika Fadlia Dunggio Fatmariani Abdul Wahab Febriyanti Febriyanti Febriyanti febriyanti febriyanti Febriyanti Febriyanti Febriyanti, Febriyanti Fitria H.Ys. Adju Frida Maryati Yusuf Gogou, Elsi Gonibala, Alfikry H. Husain , Ilyas HASAN, ANI M. Hayati Ningsih Gubali Hayatiningsih Gubali Herinda Mardin Herlina Jusuf Hilimi, Delvita Septianingsi Husain, Ilyas H. Ibrahim, Nurain Ibrahim, Rawiyanti Ilyas Husain Ilyas Husain Inaku, Irhamna Indriati Husain Intan Permatasari Irhamna Inaku Ishak Isa Ishak, Sri Layla Putri Isima , Lindawaty Kadita P. Latif Kandowangko, Novri Youla Kandowango, Novri Youla Kirsten Caroline Donsi ladoma, wafiq aziza m. Lamangantjo, Chairunnisa J. Lamangida, Novi Safitri Lamante, Nazwa S. Lilan Dama Lindawaty Isima M, Maesarah Magfirahtul Jannah Mahluk, Linda K. Mamu , Hartono D Mantali, Magfirah F. Margaretha Solang Marini Susanti Hamidun Masra Latjomph Masra Latjompoh Masrid Pikoli Mega Anastasya Diska Mokoginta Mega Rosalita Ekaputri Koni Menanti, Menanti Mestin Abdullah Miftahul F. Adudu Miftahul Fikriyah Adudu Moh G. H. Sahada Moh. Gifari Hi Sahada Moha, Tri Vinesa Mohamad Ikbal Riski Danial Muhamad Iksan Mustamin Ibrahim Mustaqimah, Nur Najmah, Najmah Netty Ino Ischak Nina Tanjung Api Novri Youla Kandowangko Nurain Ain Djafar Nurain Djafar Nurdin, St. Surya Indah Nurma Rosalia Nurul Fajryani Usman Pagala, Devi Bunga Pakaya, Melisa Patta Sija Podungge, Sandra Ramlah Ramlah Restiyani Widia Putri Risna A. Onu Rulan U. Mustapa Salihun, Agil Saliko, Abdul Mazid Sari, Wahyuningsih Ratna Sela Restia Tangahu Sidik Katili , Abubakar Sija, Patta Sintia S. Ahmad Sri Anggriyanti Putri Bohoka Sri Manovita Pateda Sukrin Ismail Syam S. Kumaji Tanaiyo, Sri Nuning B. Tandililing, Indayani Thalib, Moh. Randy Putra Pratama Tri Nugroho, Bagus Triska Wahyuni Dawit Uno, Wirnangsi Din. Vivien Novarina Kasim Walangadi, Farhana Rahmatia Widya Rahmawaty Saman Winda Minarti Jusuf Winda Minarti Jusuf Wirnangsi D. Uno Wirnangsi D. Uno Wirnangsih D. Uno Yasin, Indri Afriyani Yuliana Retnowati Yusuf, Septia Zuliyanto Zakaria