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Aktivitas Sitotoksik dan Ekspresi Protein p53 Bcl-2 Ekstrak dan Fraksi Daun Kersen (Muntingia calabura L.) terhadap Sel Kanker Payudara T47D Maulita Saraswati; Nuraini Harmastuti; Wiwin Herdwiani
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 17 No. 02 Desember 2020
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v17i2.7280

Abstract

Daun kersen (Muntingia calabura L.) merupakan tanaman yang dapat digunakan sebagai alternative pengobatan kanker. Penelitian ini bertujuan mengetahui aktivitas sitotoksik ekstrak etanol daun kersen, fraksi n-heksan, fraksi etil asetat dan fraksi air ekstrak daun kersen terhadap sel T47D dan mengetahui pengaruh ekspresi protein p53 dan Bcl-2 pada pemberian fraksi aktif daun kersen. Daun kersen diekstrak dengan metode maserasi menggunakan pelarut etanol 70%. Ekstrak etanol kemudian difraksinasi dengan partisi cair-cair. Uji sitotoksik dilakukan pada sel kanker payudara T47D dan sel vero dengan metode MTT assay dan dibaca absorbansinya menggunakan ELISA reader. Untuk mengetahui pengaruh ekspresi protein p53 dan Bcl-2 dilakukan uji imunositokimia. Hasil penelitian ini menunjukkan bahwa fraksi n-heksan daun kersen memiliki aktivitas sitotoksik potensial terhadap sel kanker payudara T47D, sedangkan pada ekstrak, fraksi etil asetat, dan fraksi air memiliki aktivitas sitotoksik moderat. Fraksi n-heksan daun kersen mampu meningkatkan ekspresi protein p53 dan Bcl-2 pada konsentrasi 6,79 - 27,16 µg/mL.
UJI AKTIVITAS SITOTOKSIK HERBA KELAKAI (Stenochlaena palustris (Burm.F.) Bedd.) TERHADAP SEL KANKER HATI HEPG2 Masyitah Novia Yanti; Ismi Rahmawati; Wiwin Herdwiani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.196 KB) | DOI: 10.29122/jbbi.v8i2.4811

Abstract

Liver cancer is a disease with a high number of cases. Kelakai herb (Stenochlaena palustris (Burm.f.) Bedd.) has cytotoxic activity against MCF-7, MDA-MB-231, DU-145 and HeLa cells. The aim of this study was to determine the cytotoxic activity of the herbal fraction of kelakai against HepG2 liver cancer cells. Kelakai herb was macerated using ethanol 96% followed by fractionation using n-hexane, ethyl acetate, and water as solvent. Cytotoxic test of the kelakai extracts and fractions were undertaken using the 3-[4,5-dimetilthiazol-2yl] 2,5-difeniltetrazolium bromide (MTT) assay method. Immunocytochemical reactions were applied to determine the expression of p53 and caspase-3 proteins in the apoptotic pathway of HepG2 cells. The results showed that the extract, n-hexane, and ethyl acetate fraction of kelakai herbs had cytotoxic activity against HepG2 cells, while the water fraction had no cytotoxic activity. Ethyl acetate fraction as the most active fraction of kelakai herbs could increase the expression of caspase-3 and p53 proteins in HepG2 cells. The ethyl acetate fraction had flavonoids, alkaloids, and tannins which are potent as a cytotoxic against HepG2 cells. Kanker hati merupakan salah satu penyakit dengan jumlah kasus yang cukup tinggi. Herba kelakai (Stenochlaena palustris (Burm.f.) Bedd.) memiliki aktivitas sitotoksik terhadap sel MCF-7, MDA-MB-231, DU-145, dan HeLa. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dari fraksi herba kelakai terhadap sel kanker hati HepG2. Herba kelakai dimaserasi dengan etanol 96%, dilanjutkan fraksinasi menggunakan pelarut n-heksan, etil asetat, dan air. Uji sitotoksik ekstrak dan fraksi herba kelakai dilakukan menggunakan metode 3-[4,5-dimetilthiazol-2yl] 2,5-difeniltetrazolium bromide (MTT assay). Reaksi imunositokimia digunakan untuk mengetahui ekspresi protein p53 dan caspase-3 pada jalur apoptosis sel HepG2. Hasil penelitian menunjukkan bahwa ekstrak, fraksi n-heksan, dan etil asetat herba kelakai memiliki aktivitas sitotoksik terhadap sel HepG2, sedangkan fraksi air tidak memiliki aktivitas sitotoksik. Fraksi etil asetat sebagai fraksi teraktif dari herba kelakai dapat meningkatkan ekspresi protein caspase-3 dan p53 pada sel HepG2. Fraksi etil asetat memiliki golongan senyawa flavonoid, alkaloid, dan tanin yang memiliki kemampuan sitotoksik terhadap sel HepG2.
Hubungan Kepatuhan Penggunaan Obat terhadap Tekanan darah dan Kualitas Hidup Peserta Prolanis Hipertensi di Kabupaten Demak Galih Kurniawan; Ika Purwidyaningrum; Wiwin Herdwiani
Jurnal Farmasi Indonesia Vol 19 No 2 (2022): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/jfi.v19i2.1704

Abstract

Optimal quality of life for people with hypertension is very important so as not to reduce patient productivity. Adherence drug use is one of the factors thought to be associated with blood pressure and quality of life. This study aims to determine the relationship between adherence to medication use on blood pressure and quality of life of patients with hypertension in Prolanis . participants at the First Level Health Facility of the Klinik Pratama. This study used a cross sectional design involving hypertensive patients participating in Prolanis who met the inclusion and exclusion criteria at seven Primary Clinics in Demak Regency. Data on medication adherence were obtained using the MARS-5 questionnaire, quality of life data using the EQ-5D-5L questionnaire, and blood pressure data using a digital sphygmomanometer. The relationship between the level of compliance with blood pressure and the relationship between the level of compliance with quality of life was tested using Spearman rho analysis. The results of the study with a total of 87 respondents showed that most of them had moderate levels of adherence (52%), systolic blood pressure (64%) and diastolic blood pressure (57%) were not controlled and the average utility score was 0.845. There is a relationship between drug use adherence to systolic and diastolic blood pressure (P = 0.009), (P = 0.032) with a weak correlation level (r = 0.27), (r = 0.23) and there is a relationship between drug use adherence on quality of life (P = 0.000) with a moderate level of correlation (r = 0.42).
Evaluation, Remedy of Waste, Lean Hospital Method, Process of Inpatient Pharmacy Services in Hospital Yunilah Sukmadryani; Wiwin Herdwiani; Tri Wijayanti
Jurnal Health Sains Vol. 4 No. 4 (2023): Journal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v4i4.886

Abstract

Each installation in the hospital must continue to evaluate, improvement, and develop, including the pharmacy installation. Pharmacy installations as one of the medical support services in hospitals are required to improve the quality of their services on an ongoing basis. This study aims to see, the root of the problem, and provide evaluation and improvement for the reading of critical waste in the inpatient pharmacy service process of Kabupaten Karanganyar Hospital. This study uses a non-experimental research design and was descriptive qualitative in nature. Data techniques in qualitative and quantitative research. The value of patient data was obtained from distributing questionnaires. The pharmaceutical service process was obtained from observation and document review, then mapping in the form of value stream mapping. The determination of waste was obtained from distributing questionnaires and in-depth interviews with the 5 why method. The results of the reasearch show critical waste on inpatient service in pharmacy installation is waste defect and waste waiting. The root cause of critical waste is the prescription of drugs by doctors outside the formulary and the root cause of waiting for waste is a prescription from the hospital admission at the same time. The proposed improvement for the cause of critical waste is Monitoring and evaluating the implementation of the formularium on an going basis, provide special software to enter patient data, and implement the use of electronic prescribing to shorten the service process time.
Uji Aktivitas Hepatoprotektor Ekstrak Etanol dan Fraksi N-Heksana, Etil Asetat, dan Air Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr.) pada Tikus Jantan Galur Wistar Putri Rinda Pratiwi; Wiwin Herdwiani; Tri Wijayanti
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 14, No 1 (2023): Januari 2023
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf14116

Abstract

Dayak onion bulbs (Eleutherine palmifolia (L.) Merr.) are plants that contain flavonoids which act as high antioxidants. The process of liver damage caused by free radicals can be prevented by the role of antioxidants. This study aims to look at the hepatoprotective effect of the Dayak onion bulbs by looking at the inhibition of SGOT and SGPT levels and looking at the decrease in MDA in vivo. This study used 35 male Wistar rats divided into 7 groups each containing 5 rats. Normal control group, negative control, positive control, ethanol extract of Dayak onion bulbs 162 mg/kgBW, n-hexane fraction at a dose of 2.79 mg/200gBW, ethyl acetate fraction at a dose of 3.06 mg/200gBW, water fraction at a dose of 4.04 mg /200gBB given for 13 days. Then on the 14th day, treatment was given with paracetamol at a dose of 2.5 g/kg BW except for normal controls. Data on levels of SGOT, SGPT, and MDA were tested using Shapiro-Wilk to determine the normality of the data, then analyzed using One Way Anova followed by the Tukey test. The results showed that the ethanol extract and fraction of n-hexane, ethyl acetate and water of Dayak bulbs can reduce levels of SGOT, SGPT and MDA in male rats induced by paracetamol. The dose that was effective in reducing SGOT, SGPT and MDA levels in male rats was the ethyl acetate fraction at a dose of 3.06 mg/200gBW.Keywords: Dayak onion bulbs (Eleutherine palmifolia (L.) Merr.); hepatoprotectors; antioxidants ABSTRAK Umbi bawang dayak (Eleutherine palmifolia (L.) Merr.) merupakan tanaman yang mengandung flavonoid yang beraktivitas sebagai antioksidan yang tinggi. Proses kerusakan hati akibat radikal bebas dapat dicegah dengan peran antioksidan. Penelitian ini bertujuan untuk melihat efek hepatoprotektor dari bagian umbi bawang dayak dengan melihat daya hambat terhadap kadar SGOT dan SGPT dan melihat penurunan MDA secara in vivo. Penelitian ini menggunakan 35 ekor tikus jantan galur wistar di bagi dalam 7 kelompok masing-masing berisi 5 ekor tikus. Kelompok kontrol normal, kontrol negatif, kontrol positif, ekstrak etanol umbi bawang dayak 162 mg/kgBB, fraksi n-heksana dosis 2,79 mg/200gBB, fraksi etil asetat dosis 3,06 mg/200gBB, fraksi air dosis 4,04 mg/200gBB diberikan selama 13 hari. Kemudian pada hari ke 14 diberikan perlakuan dengan parasetamol dosis 2,5 g/kgBB kecuali pada kontrol normal. Data kadar SGOT, SGPT, dan MDA diuji menggunakan Shapiro-wilk untuk mengetahui normalitas data, kemudian dianalisi menggunakan One Way Anova yang dilanjutkan uji Tukey. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi n-heksana, etil asetat dan air umbi bawang dayak dapat menurukan kadar SGOT, SGPT dan MDA pada tikus jantan galur wistar yang diinduksi parasetamol. Dosis yang efektif dalam menurunkan kadar SGOT, SGPT dan MDA pada tikus jantan yaitu fraksi etil asetat pada dosis 3,06 mg/200gBB.Kata kunci: umbi bawang dayak (Eleutherine palmifolia (L.) Merr.); hepatoprotektor; antioksidan
Hypoglycemic effect test of Ethanolic extract of Baligo seeds (Benincasa Hispida (Thumb.) Cogn.) on white male rats wistar strain with Alloxan induction Dina Maryati; Jamilah Sarimanah; Wiwin Herdwiani; lrfan Zamzani
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol. 11 No. 2 (2019): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3965.822 KB) | DOI: 10.35617/jfionline.v11i2.4

Abstract

Baligo merupakan tanaman asli dari daerah tropis yang di Indonesia dikenal dengan sebutan kundur atau labu besar. Baligo antara lain mengandung karbohidrat, glikosida, alkaloid, fitosterol, gum dan mucilago, saponin, protein dan asam amino bebas, serta flavonoid. Kandungan kimia pada biji baligo yang diduga memiliki aktivitas antidiabetes adalah flavonoid dan  saponin.  Penelitian  ini  bertujuan untuk mengetahui efek hipoglikemik ekstrak etanol biji baligo (Benincasa hispida (Thumb.) Cogn.) dan mengetahui apakah semakin besar dosis ekstrak etanol biji baligo (Benincasa hispida (Thumb.) Cogn.) semakin besar efek hipoglikemik pada tikus putih jantan galur wistar dengan induksi aloksan. Penelitian ini menggunakan metode induksi aloksan dimana tikus dibagi menjadi lima kelompok perlakuan yaitu masing- masing kelompok terdiri dari lima ekor tikus. KI sebagai kontrol negatif (CMC 0,5%), K II sebagai kontrol positif (glibenklamid) dengan dosis 0,09 mg/200 g BB tikus, dan K III, IV, V diberikan dosis ekstrak etanol biji baligo berturut-turut 4,10 mg/200 g BB tikus, 8,21 mg/200 g BB tikus, 16,42 mg/200 g BB tikus. Semua kelompok diinduksi aloksan pada hari ke-0 (setelah dipuasakan 16 jam) secara intraperitoneal. Pemeriksaan kadar gula darah dilakukan pada hari ke-4 dan ke-8 setelah pemberian sediaan uji. Hasil analisa statistik menunjukkan bahwa semakin besar dosis ekstrak etanol biji baligo (4,10 mg/200 g BB tikus, 8,21 mg/200 g BB tikus, 16,42 mg/200 g BB tikus) tidak semakin besar efek hipoglikemik pada tikus putih jantan galur wistar dengan induksi aloksan.
Efektivitas rimpang kunyit hitam (curcuma caesia roxb.) terhadap penurunan glukosa darah dan perbaikan ginjal tikus diabetes nefropati Zakiyyah Qurrotul Aini; Wiwin Herdwiani; Tri Wijayanti
MEDIA ILMU KESEHATAN Vol 12 No 2 (2023): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v12i2.874

Abstract

Background: Diabetes Mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action or both. Diabetic nephropathy is a degenerative kidney vascular disorder that has a relationship with impaired carbohydrate metabolism or sugar intolerance (Diabetes Mellitus). Black turmeric (Curcuma Caesia Roxb.) is widely used by the community as a traditional medicine and has potential as an antidiabetic.Objective: This study aims to determine the effect of black turmeric extract on decreasing blood glucose levels and its effect on improving kidney histopathology in diabetic nephropathy rats.Method: This research was conducted at the Laboratory of the Center for Food and Nutrition Studies, University of Gajah Mada for the period October-November 2022 consisting of 6 groups. STZ-Na induced rats to obtain a state of hyperglycemia. Examination of blood glucose levels using the GOD-PAP method (enzymatic photometric test).Results: The percentage of blood glucose is decreased levels was 16.75% and repaired kidney histopathology was 6.67% as a score mild damage category (1).Conclusion: Black turmeric rhizome extract (Curcuma Caesia Roxb.) can reduce blood glucose levels and improve kidney function at a dose of 400 mg/kg BW.
Implementation of BPOM Regulation No. 8 of 2020 Concerning Online Drug Distribution at 4 Pharmacies in Purworejo Regency, Indonesia Aminudin; Jason Merari Peranginangin; Wiwin Herdwiani
Archives of The Medicine and Case Reports Vol. 4 No. 3 (2023): Archives of The Medicine and Case Reports
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/amcr.v4i3.334

Abstract

The high number of internet users has an impact on the development of trade transaction models from conventional to electronic or online, known as e-commerce. The web-based or e-commerce business action plan in Indonesian regulations is called trading through electronic systems (PMSE). This study aimed to find out the implementation of BPOM regulation No 8 of 2020 concerning online drug distribution in 4 pharmacies in Purworejo Regency, Indonesia. This research is an observational study with a qualitative approach using information-gathering techniques through perception directly and meetings with several sources or witnesses related to this check. At the data analysis stage, the results of the interviews will be used as material for analyzed thematically by looking at and connecting with the problem formulation and research purposes. Interview data were analyzed using NVIVO 12 pro software. The implementation of BPOM Regulation No. 8 of 2020 related to the circulation of drugs distributed online in 4 pharmacies in Purworejo Regency has been running with a percentage of 79.55% in accordance with and 20.45% not in accordance with existing regulations. The implementation of BPOM Regulation No. 8 of 2020 concerning the Circulation of Drugs Distributed Online in pharmacies in Purworejo Regency has a significant impact on the supervision and safety of drug circulation through online platforms or e-commerce. In conclusion, based on the provisions contained in BPOM Regulation No. 8 of 2020 related to the circulation of drugs distributed online, most of the regulations have been implemented in the 4 pharmacies but improvements are still needed in the media used.
Lean Approach to Identifying Problems in Outpatient Pharmacy Installations at Simo General Hospital, Boyolali, Indonesia Setiaji Wisnu Wardana; Rina Herowati; Wiwin Herdwiani
Archives of The Medicine and Case Reports Vol. 4 No. 3 (2023): Archives of The Medicine and Case Reports
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/amcr.v4i3.335

Abstract

Lean approach is a method used to analyze value-added and non-value-added activities with the aim of minimizing or eliminating the waste that happens in an institution. The purpose of this research is to identify waste and find the root cause of waste that occurred in the outpatient pharmacy installation at the Simo General Hospital. This research method is non-experimental descriptive research with a qualitative descriptive exploratory approach through questionnaires, observation, and in-depth interviews with respondents. The analysis of this research is the observation of the service process flow with value stream mapping, distributing questionnaires, Borda method to search waste critically, 5why interviews to find the root cause of waste criticism and suggestions for solving problems. The results showed that the critical waste that occurred was waste waiting with a percentage value of 23.41%. The root cause of waste waiting is the accumulation of prescription queues at the preparation, dispensing delivery of drugs, and PIO stages due to a lack of pharmaceutical technical personnel. The proposed improvement in waste waiting is to increase the number of pharmacy officers by first calculating the workload.
REVIEW: PENGARUH ENKAPSULASI KURKUMIN TERHADAP AKTIVITAS ANTIOKSIDAN : REVIEW: THE EFFECT OF CURCUMIN ENCAPSULATION ON ANTIOXIDANT ACTIVITIES Syamsul Ma'arif; Jason Merari Peranginangin; Wiwin Herdwiani
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.897

Abstract

Kurkumin memiliki aktivitas antioksidan yang rentan dipengaruhi oleh cahaya, pH, suhu, oksigen dan dapat bereaksi dengan senyawa lain. Enkapsulasi dapat melindungi senyawa bioaktif. Systematic literature review (SLR) merupakan sebuah pendekatan penelitian secara sistematis untuk mengidentifikasi, mengevaluasi dan menafsirkan semua studi yang relevan mengenai topik tertentu. Penelitian SLR ini bertujuan untuk mengetahui pengaruh enkapsulasi kurkumin terhadap aktivitas antioksidan dan rekomendasi metode serta penyalut untuk enkapsulasi kurkumin. Penelitian ini menggunakan metode SLR yang dilakukan dalam beberapa tahap yaitu perencanaan (planning) dengan menentukan elemen PICO, dataset, kata kunci pencarian, kriteria inklusi dan eksklusi. Tahap pengumpulan data (conducting) meliputi skrining data dari judul dan abstrak, seleksi teks lengkap, penilaian kualitas artikel serta analisis dan sintesis data. Tahap pelaporan (reporting) melaporkan hasil review. Hasil penelitian menunjukkan enkapsulasi mampu meningkatkan aktivitas antioksidan kurkumin. Kurkumin yang dienkapsulasi mempunyai aktivitas antioksidan yang lebih baik daripada kurkumin yang tidak dienkapsulasi. Metode electrostatic complexation, dan penyalut alami laktoferin dan pektin adalah metode dan penyalut yang direkomendasikan untuk enkapsulasi kurkumin. Hal ini berdasarkan data enkapsulasi kurkumin dengan metode dan penyalut tersebut mempunyai karakteristik enkapsulasi yang baik dan aktivitas antioksidan paling tinggi. Kata kunci: Systematic literature review, Kurkumin, Antioksidan, Enkapsulasi.