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GENETIC DIVERSITY AND FRUIT QUALITY OF SEVERAL POMELO “JERUK BALI” (Citrus grandis L. Osbeck) CULTIVARS IN BALI Ida Bagus Komang Mahardika; I Nyoman Rai; Made Sudiana Mahendra; Rindang Dwiyani
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.014 KB) | DOI: 10.24843/IJBB.2017.v05.i01.p04

Abstract

This study aimed to determine the genetic diversity and fruits quality of the "Jeruk Bali" cultivars grown in Bali. This research was conducted in all regencies and city in Bali, during 2016. Furthermore, several cultivars of “Jeruk Bali” were genetically analyzed based on RAPD markers using 10 primers. Analysis of the quality of fruit is based on physical properties and chemical content. Eighteen cultivars of "Jeruk Bali" obtained have a fruit morphological character with round, short round, and piriform fruit shapes, which are red, pink, cream and white flesh color. RAPD analysis results at 53% similarity level are grouped into 5 groups. The first group was only one cultivar, the second group consisted of 13 cultivars, the third and fourth groups were only one cultivar, while the fifth group consisted of two cultivars. The analysis of the diversity between cultivars based on the combination of physical and chemical properties of the fruit with hierarchy method on similarity level about 85% in a group is obtained by 4 (four) groups. Groupings by combination of physical and chemical properties of the fruit are not synchronized in their entirety with dendograms based on their genetic diversity. This illustrates the physico-chemical properties of “Jeruk Bali” fruit in general is not fully genetical expressed, but also influenced by conditions of environmental growth.
THE EFFECTIVENESS OF INDIGENOUS ENDOMYCORRHIZA AND RHIZOBIUM INOCULUM IN INCREASING NUTRIENT UPTAKE AND YIELD OF SOYBEAN IN DRY LAND Yosni Kiuk; I Nyoman Rai; Anak Agung Istri Kesumadewi
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.738 KB) | DOI: 10.24843/IJBB.2019.v07.i01.p03

Abstract

Increasing the productivity of soybeans in dry land can be done by designing a package of fertilizer technology based on biological materials using indigenous endomycorrhiza and Rhizobium. The application of indigenous endomycorrhizal biofertilizer combined with Rhizobium is expected to be able to improve nitrogen and phosporus uptake so as to increase the productivity of soybeans in dry land. The experiment used two-factor factorial randomized block design. The first factor was dose of endomycorrhizal spores of Glomus that consisted of four levels, namely, treatment without endomycorrhiza inoculation, dose of 50endomycorrhizal spores, dose of 100 endomycorrhizal spores, and dose of 150 endomycorrhizal spores. The second factor was inoculation of Rhizobium inoculum that consisted of two levels, namely, treatment without Rhizobium inoculation and Rhizobium inoculation. The results showed that combination of 50 endomycorrhizal spores with Rhizobium inoculation gave the best effect on the number of effective root nodules per plant, endomycorrhizal infection per plant, nutrient uptake by plant, number of pods per plant, number of seeds per plant, and oven-dry weight of seeds per plant.
MORPHOLOGICAL CHARACTERS AND GENETIC VARIABILITY OF CHAMPACA IN BALI I Made Sukewijaya; Made Sudiana Mahendra; I Nyoman Rai; I Gede Rai Maya Temaja
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Bali community utilize champaca flower for offering materials and worship, besides beauty salon purposes, the SPA aromatic ingredients, essential oils, perfumes, cosmetics, and drugs. Various champaca plants in Indonesia have not been studied as one of Indonesia's biodiversity that can be used as excellent genetic resources (germplasm). The objective of the study was to determine the genetic diversity of champaca in Bali. The results revealed that 12 (twelve) champaca accession morphologically was characterized. All of accessions obtained from cultivation centers champaca in Bali. Based on the characteristic was observed by morphological characters i.e.: (a) Cempaka Putih Wilis (b) Cempaka Putih Pateh, (c) Cempaka Putih Patemon, (d) Cempaka Putih Sibang, (e) Cempaka Kuning Muda Petemon, (f) Cempaka Kuning Kecil Patemon, (g) Cempaka Kuning Besar Patemon, (h) (i) Cempaka Kuning Kecil Sibang, (j) Cempaka Kuning Tua Sibang, (k) Cempaka Kuning Muda Sibang, and (l) Cempaka Kuning Punah Sibang. Morphologically, champaca in Bali can be grouped into 4 clusters and therefore, and based on RAPD analysis champaca in Bali could be grouped into 5 clusters.
ANALYSIS ON RELATIONSHIP AMONG DENDROBIUM spp BALI BASED ON CHARACTERISTICS OF LEAVES ANATOMY Ida Ayu Putri Darmawati; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.391 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p03

Abstract

From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3rd and 5th clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.
PHENOTYPIC, GENOTYPIC CHARACTERS AND NUTRITIONAL VALUE OF SEEDLESS WANI (Mangivera caesia Jack. var. Ngumpen Bali) (A Review) I Nyoman Rai; Cokorda Gede Alit Semarajaya; Gede Wijana; I Wayan Wiraatmaja; Ngurah Gede Astawa; Ni Komang Alit Astiari
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The diversity of Mangivera caesia Jack (Balinese name: wani) in Bali was quite high. Based on the morphological characters of the fruit, 22 cultivars had been explored in the previous research (Rai et al., 2008). One of the most superior cultivar among those and very potential to be commercially developed was seedless wani (M. caesia Jack. var. Ngumpen Bali). The cultivar had specific properties that were not possessed by the others. Ninety (90) % of the total fruits produced were seedless, while the remaining (10%) has small seed. Beside that, this seedless cultivar had thick flesh, very attractive skin color (glossy yellowish green), uniformity on the size and shape of fruits, a distinctive aroma, sweet, tasty, and highly nutritious. The results of RAPD analysis of 10 wani cultivars grown in Bali showed that this seedless cultivar (Ngumpen) was grouped in to different cluster, a part from others. In comparison with 4 seeded cultivars of wani, Ngumpen cultivar had a similar nutrient content, however, it had greater fiber and a greater percentage of edible part. We concluded that the Ngumpen cultivar was a specific and unique germplasm so that should be preserved and protected.
DNA POLYMORPHISM AND GENETIC DIVERSITY OF MANGO (Mangifera sp.) GERMPLASM IN TROPICAL ISLAND Putu Suwardike; I Nyoman Rai; Rindang Dwiyani; Eniek Kriswiyanti
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.565 KB) | DOI: 10.24843/IJBB.2019.v07.i01.p05

Abstract

Creation of new superior varieties of mango through the empowerment of local genetic resources requires information about the potential properties of mango germplasm, including DNA polymorphism and its genetic diversity. This research aimed to obtain basic data on DNA polymorphism, genetic relationship, genetic similarity level, and molecular accession of Bali’s unique local mango. Sampling was conducted in four regencies in the Province of Bali. DNA preparation, PCR, and microsatellite analysis were carried out at the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Gadjah Mada University. It found 44accessions of mangoes in Bali. The test results with 10 pairs of SSR markers showed that all primers produced polymorphic loci. There were 825 amplified DNA bands. Primer of AY31 produced the highest number of loci, which were 14 loci, while AY21 produced the fewest loci, i.e., 3 loci. Forty-four mango accessions showed a genetic similarity coefficient of 0.27 to 0.97. At a coefficient of 0.27, accessions were divided into 2 major groups: group A and group B. Group A consisted of two accessions, namely, KRA-005 and BDG-006 (Mangifera foetida Lour.), which had a similar coefficient of 0.657. Group B was divided into twosmaller groups, namely, groups B1 and B2, at a similarity coefficient of 0.342. Group B1 consisted of 39 accessions, while group B2 consisted of 3 accessions. Accessions Madu Anggur, Gading, Sambuk Mengwi, Kakul and Pakel Sulangai were identified as having unique alleles.
SPORE PROPAGATION OF INDIGENOUS ENDOMYCHORIZA FROM SEVERAL ROOTING AREAS OF SNAKE FRUIT ON DIFFERENT SOIL WATER CONTENT I Nyoman Rai; I Ketut Suada; M. Praborini; I Wayan Wiraatmaja
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.053 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p09

Abstract

Cultivation of organic snake fruit in Bali which is done on dry land with the irrigation depends on rainfall and the fertilization generally only uses uncertain amount of fallen leaves, it reduces the productivity, quality, and continuity of fruit production over time. In rhizosphere of snake fruit there are various types of indigenous endomycorrhiza that potentially can be developed as biofertilizer, but as a biofertilizer, the low number of spores population for inoculum becomes a limiting factor in using indigenous endomycorrhiza. The effort that can be done are to propagate the spores by giving water stress treatment. This study aimed to obtain the best rhizosphere location of snake fruit as the source of spores and the level of soil water content to multiply the spores. The research was conducted in the green House, Farm Station, Agriculture Faculty, Udayana University at Jalan Pulau Moyo, Denpasar, from October 2017 to January 2018. The spore propagation used nested experiment with Randomized Block Design patterns. The treatments were rhizosphere location as the source of indigenous endomycorrhizas spore consists of three levels (Bebandem District Karangasem Regency, Payangan District Gianyar Regency, and Pupuan District Tabanan Regency) and soil water content consists of three levels (100%, 70%, and 40% field capacity). The results of the research showed that the source of indigenous endomychorrizae from snake fruit rhizosphere in Payangan District gave the highest number of spores found and the highest percentage of the spore increase after propagation. While in the soil water content treatments, the highest number of spores found and the percentage of the spore increase after propagation were obtained at soil water content of 40% field capacity. The percentage of root infections from different sources of indigenous endomychorrhizae and different levels of soil water content is same i.e 100%.
GENETIC DIVERSITY OF GUAVA (Psidium Guajava L.) IN BALI INDONESIA BASED ON RAPD MARKER Ni Nyoman Ari Mayadewi; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The aim of this study was to investigate genetic diversity and relationship of guava (local name: jambu biji) genotypes grown in Bali, Indonesia, based on Random Amplified Polymorphic DNA (RAPD) markers. Twelve guava cultivars namely Jambu biji ‘Australia’, ‘Bangkok Merah’, ‘Bangkok Putih’, ‘Cokorde’, ‘Dadu 1’, ‘Dadu 2’, ‘Getas Merah’, ‘Kamboja’, ‘Kristal’, ‘Pipit’, ‘Susu’ and ‘Variegata’ were collected from nine Regencies in Bali using survey and explorative method. Ten decamers RAPD primers were employed to distinguish between the 12 cultivars and determine their genetic relationships. A dendogram was constructed using coefficient dissimilarity analysis based on phylogenetic analysis using parsimony (PAUP). The twelve cultivars were grouped into 2 main clusters and five smaller clusters. Variation between genotypes of guava local will be good sources for future crop improvemen.
PELATIHAN DAN STUDI BANDING UNTUK MENINGKATKAN KEMAMPUAN SDM LOKAL DESA SIBETAN DALAM MENGELOLA AGROWISATA SALAK I Nyoman Rai -
Buletin Udayana Mengabdi Vol 19 No 2 (2020): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Desa Sibetan yang terletak di Kecamatan Bebandem, Kabupaten Karangasem, telah terkenal sebagai pusat produksi salak di Bali. Salak telah dibudidayakan secara turun-temurun oleh mayoritas penduduk dan telah menjadi bagian penting dari ekonomi dan kesejahteraan masyarakat setempat. Salak telah berhasil diolah menjadi berbagai varian produk seperti kulit buah salak menjadi teh salak, biji salak menjadi kopi salak, daging buah salak menjadi kurma salak, pia salak, dan wine salak, rebung salak menjadi kari salak dan tumis salak, dan lain-lain. Selain terkenal dengan ikon salaknya, Desa Sibetan juga memiliki pemandangan alam yang indah, udara sejuk dan segar, dan aksesibilitas yang mudah karena lokasinya yang dekat dengan berbagai tujuan wisata yang telah dikembangkan sehingga sangat potensial untuk dikembangkan sebagai obyek agrowisata. Hingga saat ini belum banyak yang tahu bahwa Desa Sibetan memiliki banyak varietas plasma nutfah salak yang dapat digunakan untuk mendukung pengembangan agrowisata. Kegiatan pengabdian masyarakat yang didanai oleh Kementerian Riset, Teknologi, dan Pendidikan Tinggi melalui “Program Pengambangan Desa Mitra/PPDM” Tahun III, dilakukan untuk meningkatkan kemampuan sumberdaya manusia lokal dalam mengelola agrowisata berbasis salak. Kegiatan dilakukan dari April-Agustus 2019. Implementasi kegiatan menggunakan pendekatan bekerja dengan masyarakat sasaran dan pembelajaran melalui penerapan metode Pengembangan Kapasitas Kewirausahaan (ECB) dan Transfer Teknologi (TT). Jenis kegiatan yang dilakukan adalah pelatihan dan studi banding pengelolaan agrowisata, pelatihan menjadi cooking lesson, pemasaran produk hasil olahan salak, dan pembuatan web-site agrowisata salak Sibetan. Pelatihan manajemen agrowisata dan menjadi instruktur cooking lesson dilakukan secara terpadu dengan kegiatan studi banding ke Obyek Agrowisata Aditya Jaya, di Banjar Padangaling, Desa Cau Belayu, Marga, Tabanan. Peserta pelatihan adalah semua peserta studi banding yang terdiri atas perwakilan anggota Kelompok Tani Kertha Semaya, anggota KWT Agro Abian Salak, anggota Kelompok agrowisata Agro Abian Salak, perwakilan Kelihan Dusun dan perwakilan Karang Taruna Desa Sibetan melalui metode learning by doing. Hasil pelatihan menunjukkan bahwa para peserta mengikuti kegiatan dengan antusias yang ditunjukkan oleh keseriusan mereka bertanya dan berdiskusi serta bersemangat dalam mengikuti setiap tahapan pelatihan. Melalui metode learning by doing mereka menyatakan bahwa dapat dengan mudah mengerti dan memahami bagaimana melakukan manajeman agrowisata dan menjadi instruktur cooking lesson yang baik karena diberikan contoh nyata sehingga hal tersebut nantinya dapat dengan mudah diadopsi untuk kondisi di tempat mereka di Desa Sibetan. Pemasaran produk hasil olahan salak dilakukan ke berbagai tempat di pasar tradisional, toko modern dan swalayan di seputaran kota Karangasem dan Denpasar, sedangkan pembuatan web-site ditujukan sebagai sarana promosi agrowisata salak Sibetan agar dikenal secara nasional dan internasional.
ANALISIS HARA, TOTAL MIKROBA, SERTA PRODUKSI PADI PADA BUDIDAYA SYSTEM OF RICE INTENSIFICATION (SRI) DAN TRADITIONAL FLOODING (TF) Ni Putu Giyan Adnya Antari; I Nyoman Rai; I Wayan Budiarsa Suyasa
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 13 No 1 (2019)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.32 KB) | DOI: 10.24843/EJES.2019.v13.i01.p10

Abstract

The conventional rice cultivation of Traditional Flooding (TF) seems to be wasting the water and potentially polluting the soil and water due to improper chemical fertilizers and pesticides management (Nguyen et al., 2007). System of Rice Intensification (SRI) is one of the innovative rice cultivations with the principle of single seed replantation and intermitten irrigation (IWMI, 2007). The experiment was conducted to analyze the macro nutrients, total microbes, and the rice production by combinations of nutrient and pest management in TF and SRI system. This study applied a nested experimental design with two factors. The first factor was the cultivation system that consisted of two levels (TF and SRI), and the second factor was nutrient and pest management consisted of three combination levels (synthetic nutrient and pest management/P1, organic-synthetic nutrient and organic pest management/P2, as well as organic nutrient and pest management/P3). These combinations were nested to TF and SRI. Data were analyzed by ANOVA. It is found that P1 showed the highest growth and production in SRI and TF. The numbers of the height, tillers, total grains and the weight of seed per pot in SRI respectively are 87.33 cm, 22.17 tillers, 1179.50 grains, and 32.17 g, meanwhile in TF respectively are 78.75 cm, 17.50 tillers, 1063.33 grains and 29.00 g. P3 shows the highest content of macro nutrients and total microbes in SRI and TF, respectively the contents of C, N, P and K of P3 in SRI are 6.82 % (very high), 0.31 % (moderate), 155.69 ppm (very high), and 1804.85 ppm (very high), meanwhile in TF respectively are 6.52 % (very high), 0.25 % (moderate), 52.60 ppm (very high), and 530.79 ppm (very high). The total microbe contents of P3 in SRI and TF respectively are 2.7 x 108 CFU/g and 3.7 x 108 CFU/g. It is concluded that both in TF and SRI system (1) P1 shows the highest growth and production and (2) P3 shows the highest content of macro nutrients and total microbes. Furthermore, it is suggested that in the future the rice cultivation system should be done in a wider scale and multiple planting seasons in order to be able to precisely measure the significant difference of the rice cultivation system towards the rice growth and production.
Co-Authors A.A.P Agung Suryawan Adriana Hiariej, Adriana Agus Sutanto Agus Sutanto Anak Agung Istri Kesumadewi ANELIA REZKINA BR.S Bambang S. Purwoko Bambang Sapta Purwoko Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DEWA AYU BULAN INDRAYUNI, DEWA AYU BULAN Dharma, IGB Sila DIPUTRA, I MADE MARTANA ENIEK KRISWIYANTI Faisal Siregar Fau, Maria Berlian Fenny M. Dwivany FENNY MARTHA DWIVANY Fina Elziana Sapary G Wijana GEDE WIJANA GEDE WIJANA Giasintha Stefani Gusti Ngurah Alit Susanta Wirya Hendri, Jajang Husna Nugrahapraja I G. R. M. TEMAJA I GEDE JAYA MAHENDRA I GEDE KRISNA PRATAMA PUTRA I Gede Mahardika I GEDE SUDARMIKA I GUSTI ALIT GUNADI I GUSTI AYU ARINI LAKSEMI I Gusti Ayu Kadek Dian Permatha Suyoga I Gusti Ayu Kadek Dian Permatha Suyoga I GUSTI NGURAH SANTOSA I KADEK DWI MAHARDIKA I KADEK EKADANA I Ketut Suada I KETUT SUNARKA I MADE MARTANA DIPUTRA I Made Sara Wijana I Made Sudana I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I Nyoman Dibia I NYOMAN GEDE ASTAWA I Nyoman Gede Ustriyana I Nyoman Sunarta I Nyoman Wahyu Ardianta I Nyoman Wahyu Ardianta I NYOMAN WIJAYA I PUTU DHARMA I PUTU DIAN PRATAMA I PUTU SUDANA I W BUDIARSA SUYASA I Wayan Diara I WAYAN EKA SAPUTRA I Wayan Nuarsa I WAYAN RUMADA I Wayan Sandi Adnyana I WAYAN SUAMBA I WAYAN WIRAATMAJA I WAYAN WIRATMAJA I.G.K. Dana Arsana IDA AYU ASTARINI IDA AYU MAYUN Ida Ayu Putri Darmawati Ida Bagus Komang Mahardika Ida Bagus Komang Mahardika IGK Dana Arsana KADEK JUNIARI Karlia Meitha ketut asrini Ketut Wikantika Khusni, Mazroatul Latifah K. Darusman Latifah Kosim Darusman Liran, I Gusti Putu Oka Maha Putra Wardana Luh Kartini Luh Putu Ratna Sundari M. Praborini M. Sudiana Mahendra Made Sudiana Mahendra, Made Mahardika, Ida Bagus Komang MARLIN MARHAENI PE Melani Jelita Tarigan Mintarajasa, Jaya Duarsa Muhammad Rivai NADA SAHARA NGAKAN MADE ADI WEDAGAMA Ngurah Gede Astawa NI KADEK ARYANI NI KADEK EMA SUSTIA DEWI Ni Kadek Trisnayanti Ni Kadek Trisnayati Ni Komang Alit Astiari Ni Komang Alit Astiari Ni Komang Ayu Septiani Ni Luh Kartini NI LUH WIDYASARI NI MADE KESUMA DEWI Ni Made Tia Juliasari NI MADE TRIGUNASIH NI NYOMAN ARI MAYADEWI Ni Putu Anom Sulistiawati Ni Putu Anom Sulistiawati Ni Putu Anom Sulistiawati, Ni Putu Anom NI PUTU ARYANTI Ni Putu Giyan Adnya Antari Niken Prameswari Putri Nisrina Sukriandi Onesimus Ke Lele Putu Suwardike Rindang Dwiyani Roedhy Poerwanto Roedy Poerwanto SAKHARA ZADE Shinta Lestari Santosa Sila Dharma, IGB SITI SHOFIAH Suwardhi, Deni Topik Hidayat UTAMA, I WAYAN EKA KARYA UTAMI UTAMI UTAMI UTAMI Wayan Trisna Dewi Wedayani, Ni Made WIDHIANTHINI WIDHIANTHINI Wiraatmaja, Wayan Yohanes , Setiyo Yohanes Setiyo Yoshua Aurelio Tarigan Yosni Kiuk YUDHA, I KADEK WISMA Yuyun Fitriani