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Journal : Buletin Peternakan

Polymorphism and Association of the Novel KCTD2 Gene with Flavor and Odor in Indonesian Local Sheeps Herpina Herpina; Kasita Listyarini; Cece Sumantri; Katrin Roosita; Asep Gunawan
Buletin Peternakan Vol 47, No 1 (2023): BULETIN PETERNAKAN VOL. 47 (1) FEBRUARY 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i1.80711

Abstract

A candidate marker that influences the flavor and odor of Indonesian sheep that is the Potassium Channel Tetramerization Domain Containing 2 (KCTD2) gene. This study aims to examine the polymorphism and association between the KCTD2 gene and lamb flavor and odor in Indonesian local sheeps. This study used DNA taken from the longissimus dorsi (LD) muscle of 100 rams including 75 Javanese Thin-Tail sheep (JTTS), 10 Javanese Fat-Tail Sheep (JFTS), and 15 Jonggol Sheep (JS), with ranged in weight from 20 to 35 kg and were 10 to 12 months old. The PCR-RFLP technique and GLM test analysis were used to identify polymorphisms and association of KCTD2 gene. The restriction enzyme BafI digests the PCR-RFLP product of the KCTD2 gene. The results showed that the KCTD2 gene was polymorphic (CC and CT). KCTD2 gene analysis showed a significant (P<0.05) association with 3-Methylphenol (MP). This research provide information regarding the role of the KCTD2 gene in lamb flavor and odor, especially in 3-Methylphenol (MP) compounds, and explain KCTD2 as a functional gene in the selection of premium sheep with low flavor and odor in lamb meat.
Identification of AHSG gene polymorphism and association with flavor and odor compounds of Indonesian lamb meat Septiyawan, Ganda Adi; Listyarini, Kasita; Noor, Ronny Rachman; Roosita, Katrin; Gunawan, Asep
Buletin Peternakan Vol 49, No 1 (2025): BULETIN PETERNAKAN VOL. 49 (1) FEBRUARY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i1.89123

Abstract

Flavor and odor are important factors for consumers to consider the quality of lamb meat. The AHSG Alpha2-Heremans- Schmid Glycoprotein gene is associated with a fatty acid content and is indicated as a gene controlling flavor and odor compounds. The research aims to identify the AHSG gene polymorphism and its association with flavor and odor compounds in Indonesian lamb meat. The sample used in this study was 105 ram samples consisting of 10 Javanese Fat-Tailed Sheep (JFTS), 80 Javanese Thin-Tailed Sheep (JTTS), and 15 Jonggol sheep (JS). The AHSG gene polymorphism was validated using the PCR-RFLP technique with the EagI enzyme EagI. The association of the AHSG gene SNP (g.198655287 G>A) with odor and flavor was analyzed using the T-tests. The AHSG gene polymorphism in Indonesian lamb was polymorphic with three genotypes, i.e., GG, GA, and AA, and in the Hardy-Weinberg equilibrium. The AHSG significance was associated (P<0.05) with skatole compounds (MOA, EOA, MNA, and MI). The AA genotype is recommended for a candidate marker of flavors and odors because it associated with low compound values. The AHSG gene, specifically the SNP g.198655287 G>A, can potentially serve as a genetic marker for lamb meat production by reducing flavor and odor.
Co-Authors Adha, As Syaffa Amalia Agik Suprayogi Ahmad Sulaeman Alfiah, Elma Ali Khomsan Anggar Pamungkas Anggiruling, Dwikani Oklita Aprilyana, Lia Armina Puji Utari Asep Gunawan Aulia Ratnadianti Suharjo Balgis, Yayik Dwi Bibi Ahmad Chahyanto Budi Iman Santoso Cahyo Budiman Cece Sumantri Clara M. Kusharto Clara M. Kusharto Clara M. Kusharto Clara M. Kusharto Clara M.Kusharto Clara Meliyanti Kusharto Damayanthi - Damayanthi -, Damayanthi Ekowati Handharyani Evy Damayanthi Fathimah, Fathimah Firdaus Firdaus Firdaus Firdaus Firmansyah, Hardi Fitria Nurjanah Hadi, Riyadi Hardi Firmansyah Hardinsyah Harna, Harna Herpina Herpina Hidayaturrahmah Hidayaturrahmah Huda Shalahudin Darusman I Gusti Bagus Wiksuana Ikeu Ekayanti Ilmi, Ibnu Malkan Bakhrul ITA DJUWITA Ita Djuwita Kerisnawati, Titi Khofsah, Asy Laeli Nur Hasanah Lilik Kustiyah Lilik Kustiyah Listyarini, Kasita Masruroh Mastin Mohamad Rafi Mokhamad Fahrudin Muh Asrul Irawan, Andi Muhamad Rizal Martua Damanik Muhammad Suhendra, Muhammad Mukhlidah Hanun Siregar Ni&#039;ma Hilyatin Noni Eka Jaya Wardani Novriyandi Hanif Nur Khoiriyah Nuraelah, Almira Nurjanah, Hanna Prita Dhyani Swamilaksita Rahmawati Rahmawati Ratih Putri Damayati Ratna Sholatia Harahap Rian Diana Rimbawan , Rimbawan R Risti Rosmiati Ronny Rachman Noor Rudi Heryanto Sa'pang, Mertien Septiyawan, Ganda Adi Setyaningsih, Sulasyi Siti Sa’diah Sri Anna Marliyati Syah, Muh Nur Hasan Utari Yunitaningrum Venny Agustiani Mahardikawati Widhi, Anisa Sekar Yekti Hartati Effendi Yuli Fitriyani Yulianti Wulan Sari Zakiudin Munasir Zakiudin Munasir