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All Journal HAYATI Journal of Biosciences PROSIDING SEMINAR NASIONAL JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia Jurnal Matematika & Sains Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marine Research in Indonesia Jurnal Segara ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Jurnal Perikanan
Agus Sabdono
Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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CADMIUM REMOVAL BY A BIOREDUCPIUN CORAL BACTERIUM Pseudoalteromonas sp. STRAIN CD15 ISOLATED FROM THE TISSUE OF CORAL Goniastrea aspera, JEPARA WATERS Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 13, No 2 (2010): Volume 13, number 2, Year 2010
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Seventeen strains of bacterial symbionts which are resistant to heavy metals, were isolated from the tissue of coral Goniastrea aspera, from Awur Bay, Jepara Waters. Screening procedures involving solid and liquid synthetic media containing Cd2+, resulted in the selection of seven Cd resistant strains, which showed a quantitative removal of Cd2+  by range of 68-90% of the initial Cd2+  concentrations (5 ppm) tested. One of these strains, CD15, was selected further to examine its molecular and physiological characteristics. Based on 16S ribosomal DNA sequencing and microbial characterization, the CG15 isolate is closely related to Pseudoalteromonas sp. This is the first report on the natural Cd metal tolerance levels of coral bacteria    
ANTIBACTERIAL ACTIVITIES OF BACTERIAL SYMBIONTS OF SOFT CORAL Sinularia sp. AGAINST TUBERCULOSIS BACTERIA S. Sulistiyani; Sri Achadi Nugraheni; Ocky Karna Radjasa; Agus Sabdono; Miftahuddin Majid Khoeri
JOURNAL OF COASTAL DEVELOPMENT Vol 14, No 1 (2010): Volume 14, Number 1, Year 2010
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Abstract

Tuberculosis (TB) is caused by Mycobacterium tuberculosis. Although TB is a curable disease, it continues tobe one of the most important infectious causes of death worldwide. Indonesia ranks 3rd on the list of TB highburden countries in the world with 86,000 cases deaths and the Multi Drug Resistant Tuberculosis (MDR TB)estimated cases in Indonesia is 10,000. This research was aimed to isolate and characterize of soft coralSinularia sp.-associated bacteria having antibacterial activity against Tuberculosis bacteria. There were109 isolates collected from Sinularia sp. Two isolates from Sinularia sp.-associated bacteria, SC4TGZ3 andSC4TGZ4 were successfully screened for antibacterial against Tuberculosis bacteria. SC4TGZ3 was found toinhibit the growth of MDR TB strain HE, MDR TB strain SR and H37Rv. Whereas, SC4TGZ4 was found toinhibit the growth of MDR TB strain HE. Based on PCR amplification 16S rDNA softcoral bacateria wereidentified as follows: SC4TGZ3 was closely related to Pseudovibrio sp. and SC4TGZ4 was closely related toAlpha proteobacterium sp.
NATURAL BIODEGRADATION OF COPROSTANOL IN AN EXPERIMENTAL SYSTEM OF THREE ENVIRONMENTAL CONDITIONS OF JAKARTA WATERS, INDONESIA Tonny Bachtiar; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 8, No 1 (2004): Volume 8, Number 1, Year 2004
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Abstract

Constraint of using bio-indicator (coliform bacteria) as an indicator of domestic (sewage) pollution in the environment with high environmental stress encourages the discovering of other alternate indicators. Coprostanol has been proposed as a chemical indicator of domestic waste pollution, but most research on were conducted in the temperate (high latitude) region. The persistence of coprostanol in tropical region, especially in Indonesia, is still very poor. It is very important to understand the persistence of coprostanol in the nature, as one of the requirements to propose coprostanol as an alternate indicator of domestic waste pollution. In order to better understand the natural biodegradation of coprostanol, experimental system on three environmental conditions (river, river mouth, and coastal waters) was conducted. In April 2004, samples of water and surface bottom sediments were collected from each environmental condition in duplicate. Before the samples were put into aerated and non-aerated aquaria, about 35-40 g of surface bottom sediments were taken to analyze the initial concentration (C0) of coprostanol. The sediments were subsequently sampled from each aquarium within a certain interval day to analyze the concentration of coprostanol (C10, C20, and C40). The results showed that aeration plays not an important role in natural biodegradation of coprostanol. In average, the highest rate of coprostanol biodegradation is 0.438 μg/g day-1 in non aerated coastal water environment, where as the lowest was found in the non aerated river mouth environment (0.021 μg/g day-1). Since coprostanol was degraded very slowly, and could be detected in the sediments of three environmental conditions, coprostanol has an excellent potency to be used as an alternate indicator of domestic wastes.
CHARACTERIZATION AND IDENTIFICATION OF STRAIN KM221, A NOVEL MCPA HERBICIDE-DEGRADING BACTERIUM ISOLATED FROM CORAL SURFACE, MENJANGAN KECIL ISLAND, KARIMUNJAWA Agus Sabdono; Ocky Karna Radjasa; Joedoro Soedarsono
JOURNAL OF COASTAL DEVELOPMENT Vol 6, No 3 (2003): Volume 6, Number 3, Year 2003
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

In this study, bacterial strain KM221 was isolated from coral   tissue in Menjangan Kecil Island, Karimunjawa, Indonesia. This strain is  facultative anaerobic  with MCPA (2-methyl-4-chlorophenoxy acetic acid) serving as the only known energy sources.  Microscopy  of isolate revealed that strain KM221 is gram-positive, catalase-positive,  rod, spore-forming bacterium, motile,  opaque, hair-like outgrowth and unpigmented colonies. The bacterium could not be identified on the basis of its carbon-source-utilization pattern, but a partial sequencing of the 16S rDNA analysis suggest that this strain is closely related to Bacillus iodinum.The ability to degrade MCPA herbicide was examined qualitatively in EMBA indicator medium.  This bacterium grew exponentially with MCPA as  the sole source of energy and carbon. The maximum growth rate (m max)  and the  saturated concentration on MCPA (Cs) were determined to be  0.8024 h-1 and  5.10 mg/l MCPA, respectively.
ANTIBACTERIAL ACTIVITY OF A PIGMENT PRODUCING-BACTERIUM ASSOCIATD WITH Halimeda sp. FROM ELAND-LOCKED MARINE LAKE KAKABAN, INDONESIA Ocky Karna Radjasa; Leenawaty Limantara; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 12, No 2 (2009): Volume 12, Number 2, Year 2009
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Abstract

A pigment producing-bacterium associated with green alga Halimeda sp., was successfully isolated from a land-locked marine lake Kakaban, East Borneo, Indonesia and screened for an antibacterial activity against pathogenic Staphylococcus aureus. The bacterium was identified as Pseudoalteromonas piscicida based on its 16S rDNA and was found to produce xanthophyll pigments and to amplify gene fragments of Non-ribosomal peptide synthetase (NRPS).
ETHICAL PERSPECTIVES OF SUSTAINABLE USE OF REEF’S INVERTEBRATES AS A SOURCE OF MARINE NATURAL PRODUCTS S Sukarmi; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 11, No 3 (2008): Volume 11, Number 1, Year 2008
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Abstract

Among marine animals, reef’s invertebrates are the most prolific producers of secondary metabolites and have become sources of great interest to natural product chemistry, since they provide a large proportion of bioactive compounds with different biological activities. Supply’s problem has hampered the investigation of secondary metabolites from marine invertebrates, and  many highly active compounds produced contribute to<10-6 % of the body-wet weight. Providing sufficient amounts of these biologically active substances, hence, may be a difficult task. In addition, it has often proven extremely difficult, and some cases impossible, to provide from invertebrates sufficient amounts of many of these substances due to limited amounts found in the producing organism, or to limited quantity of the organism itself, or to geographic, seasonal or sexual variations in the amounts and in the nature of produced secondary metabolites. There has an increasing concerns regarding the collecting reef’s organisms for the discovery and development of pharmaceuticals since it has been perceived variously as sustaining and threatening conservation. There is an urgent need to take into account the bioethical considerations in anticipating the potential consequences of these activities and proposing management options for sustainable use of reef’s invertebrates as the sources of bioactive compounds.
BIODEGRADATION OF CHLORPYRIFOS BY A MARINE BACTERIUM Bacillus firmus Strain BY6 ASSOCIATED WITH BRANCHING CORAL Acropora sp. Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 2 (2007): Volume 10, Number 2, Year 2007
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Abstract

A bacterium which utilizes chlorpyrifos as a sole source of carbon and energy was isolated from coral surface. The organism utilized chlorpyrifos up to 25 mg l-1. The lag phase and time for degradation, however, were severely prolonged at chlorpyrifos concentrations above 10 mg l-1 . The growth of kinetics of coral bacterium was studied in batch culture. Estimation of maximum growth rates (μmax), obtained from turbidity measurements, was 0.14 h-1 and half-saturation growth constant (Cs)   was 9.34 mg l-1 chlorpyrifos. This strain demonstrated greatest similarity to members of the order Bacillales and was closest to members of the Bacillus firmus group.
AN ASSESSMENT OF HEAVY METAL CONCENTRATIONS IN THE SCLERACTINIAN CORAL TISSUES OF KARIMUNJAWA ARCHIPELAGO, INDONESIA Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 13, No 1 (2009): Volume 13, Number 1, Year 2009
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Abstract

Karimunjawa archipelago, marine national park, has been considered to be a relatively pristine area that have more than 90 species of corals. This study was conducted to detect any concentrations heavy metal levels in the  tissues of  eight  coral species. Based on life-forms, selected coral species are classified as massive (Porites lutea and Goniastrea  retiformis), submassive (Galaxea fascicularis and  Stylophora pistillata), foliaceous (Pavona decussata and Montipora foliosa) and branching/ramosa (Acropora aspera and Pocillopora damicornis). The concentration of heavy metals in the coral tissues  were assessed using Atomic Absorption Spectrophotometer (AAS) technique. The present experimental results demonstrated that metal in coral tissues were no significant difference between sites. Concentration levels of five heavy metals were found to be in the order of  Pb>Zn>Cr>Cd>Cu.  Relating heavy metal  concentrations to life-form corals, there were no significant differences of all the metals (except Pb), the highest concentration of Pb were found in foliaceous type of corals. The high level of Pb in foliaceous coral life-form (Pavona decussata and  Montipora foliosa), indicating that these coral species are usefull as  potential candidates of biomonitoring material for this metal.  However, the use of corals as biomonitors should be  considered carefully in biological  and local environmental factors.  
CORALS AS SOURCE OF BACTERIA WITH ANTIMICROBIAL ACTIVITY ocky radjasa; Jutta Wiese; Agus Sabdono; Johannes F Imhoff
JOURNAL OF COASTAL DEVELOPMENT Vol 11, No 3 (2008): Volume 11, Number 1, Year 2008
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Abstract

In this study we examined marine bacteria associated with different corals (Porites lutea, Galaxea fascicularis, Acropora sp. and Pavona sp.) collected from vicinity of Panjang island, Jepara, North Java Sea, Indonesia for their antimicrobial activities against the bacteria Echerichia coli, Bacillus subtilis, Staphylococcus lentus and the yeast Candida glabrata. A total of 13 bacterial isolates belonged to the members of Bacillus, Vibrio, Micrococcus, Pseudoalteromonas, Arthrobacter and Pseudovibrio were found to inhibit the growth of at least one test strain. Further examinations among the biologically active strains by using PCR with specific primers of non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) resulted in the presence of NRPS gene fragments in the 2 members of Bacillus and Micrococcus and PKS gene fragments in the 2 members of Bacillus and Vibrio. Following cloning and sequencing of the PCR products, the fragments from Bacillus BM1.5 and Micrococcus BJB showed sequence identity with peptide synthetase genes of Bacillus subtilis (61 %) and Actinoplanes teichomyceticus (62.4%). On the other hand, PKS-amplifying strains Bacillus BJ.7 and Vibrio MJ.5 showed closest sequence identity with polyketide synthase genes of Bacillus subtilis (73%) and Anabaena sp 90 (62%), respectively.
SCREENING OF SECONDARY METABOLITE-PRODUCING BACTERIA ASSOCIATED WITH CORALS USING 16S rDNA-BASED APPROACH Ocky Karna Radjasa; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 7, No 1 (2003): Volume 7, Number 1, Year 2003
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Abstract

A total of 42 coral-associated bacteria were screened for their ability to produce secondary metabolites by using 16S rDNA-based approach. A specific oligonucleotides primer was used to amplify DNA non-ribosomal peptide synthetase (NRPS). Five strains were found to carry the NRPS gene and successfully inhibit the growth of other coral-associated and pathogenic bacteria. The phylogenetic characterization revealed that four strains belonged to currently known species Pseudoalteromonas luteoviolacea, Vibrio tubiashi, Vibrio carchiariae and Bacillus iodinum. On the other hand, one strain was apparently closely related with an unknown group of the gamma Proteobacteria, which was only recognized as uncultured clone 141H2.
Co-Authors Agus Indarjo Agus Trianto Agus Trianto Agus Trianto Agus Triyanto Agustina Agustina Agustina Aiyen Tjoa Aldion Adin Nugroho Ali Djunaedi Ali Djunaedi Ali Ridlo Ambariyanto , Ambariyanto Ambariyanto Angelina Ferawaty Siregar Angelina Ferawaty Siregar Aninditia Sabdaningsih Azizi, Muhammad Faris B Tyas Susanti B. Tyas Susanti Bambang Yulianto Baskoro Rochaddi Bhaskoro Rochaddi Burhan Habibi Yunus Chrisna A Suryono Chrisna A Suryono Chrisna A Suryono Chrisna Adhi Suryono Chrisna Adhi Suryono Delianis Pringgenies Denny Nugroho Sugianto Desy Wulan Triningsih DIAH AYUNINGRUM Diah Ayuningrum, Diah Diah P Wijayanti Diah P Wijayanti Diah Permata Wijayanti Diah Permata Wijayanti Diah Permata Wijayanti Dio Dirgantara Duhita Sinidhikaraning Kencana Eko Irwanto Elena Zocchi Endang Sri Lestari Erwin Ivan Riyanto Erwin Ivan Riyanto Eunike Dorothea Hutapea Farrastasya Muflihul Azzami Fauziah Shahul Hamid Gina Saptiani Gina Saptiani Hadi Endrawati Haeruddin Haeruddin Hakim, Muhamad Fikri Hudi Nur Hans Arthur Philips HANS- PETER GROSSART Hans-Peter Grossart Herawati Sudoyo Herida, Azalia Puspa Hermin Pancasakti Kusumaningrum Heru Kurniawan Alamsyah Ibnu Pratikto Ida Ayu Putu Sri Widnyani Intan Budi Setiasih Irwani Irwani Irwani Irwani Irwani Irwani Ita Widowati JOEDORO SOEDARSONO Johannes F Imhoff Jusup Suprijanto Jusup Suprijanto Jutta Wiese Larasati, Stefanie Jessica Henny Leenawaty Limantara Mada Triandala Sibero Mada Triandala Sibero Meinhard Simon Miftahuddin Majid Khoeri Misbakul Munir Muchlissin, Sakti Imam Muhamad Fikri Hudi Nur Hakim Muhamad Ziaul Faiz Muhammad Eka Darmawan Rafsanjani Muhammad Zainuri Nadya Cakasana Norma Afiati Norma Afiati Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Ocky Karna Radjasa Ocky Karna Radjasa ocky radjasa Popi IL Ayer Prastyo Abi Widyananto Puspa Kapinangasih Putut Har Riyadi Raden Ario Rafsanjani, Muhammad Eka Darmawan Ragil Saptaningtyas Raja Aditya Sahala Siagian Ratna Diyah Palupi Retno Hartati Rignolda Djamaludin Rina Setyowati Sulistiyoningrum Rini Pramesti Rivan Novianto Madilana Romadhon Romadhon Rory Anthony Hutagalung Rosa Amalia Rudhi Pribadi Rudiger Stöhr S Sukarmi S. Sulistiyani Sakti Imam Muchlissin Sakti Imam Muchlissin Sakti Imam Muchlissin Setiasih, Intan Budi Setyani, Wilis Ari Sibero, Mada Triandala Slamet Budi Prayitno Sri Achadi Nugraheni Sri Lintang Artono Sri Redjeki Stalis Norma Ethica Stefanie Jessica Henny Larasati Subagiyo Subagiyo Subagiyo Subagiyo Subagyo Subagyo Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sunaryo Sunaryo Suzana Kristy Satriani Fofied Syauqina Nashihi Aufar Thorsten Brinkoff Tonny Bachtiar TONNY BACHTIAR Tony Bachtiar Tony Bachtiar Torben Marten TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Ulfah Amalia Wahyuningsih, Candra Widy Febriansyah Wilis Ari Setyani Yeni Sulistiyani Yesaya Putra Pamungkas