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All Journal HAYATI Journal of Biosciences PROSIDING SEMINAR NASIONAL JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia Jurnal Matematika & Sains Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marine Research in Indonesia Jurnal Segara ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Jurnal Perikanan

Agus Sabdono

Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro

Author-ID : 367511

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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Karakterisasi dan Identifikasi Bakteri Simbion Karang Goniastrea aspera Resisten terhadap Logam Berat Copper (Cu) dari P. Panjang, Jepara Agus Sabdono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 3 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Studi komunitas mikroba yang berasosiasi dengan karang Goniastrea aspera di P. Panjang, Jepara dilakukan dengan menggunakan teknik mikrobial molekuler berbasis kultur dependen. Tujuan dari penelitian ini adalah untuk mengisolasi, menyeleksi dan mengidentifikasi bakteri simbion karang yang resisten terhadap logam Cu. Pola toleransi terhadap logam Cu ketujuhbelas isolat dilakukan dengan menggunakan metode difusi agar. Isolat GN10 diseleksi untuk studi lebih lanjut karena memiliki karakter yang paling resisten terhadap logam Cu yang selanjutnya dikarakterisasi secara fisiologis dan molekuler. Analisis sekuen gen subunit kecil (SSU) rRNA menunjukkan bahwa isolat GN10 memiliki kekerabatan terdekat dengan Virgibacillus marismortui Strain 123 dengan derajat kesamaan 99%. Bakteri V. marismortui strain GN10 ini merupakan kandidat yang dapat digunakan sebagai indikator uji dan memiliki potensi di dalam penerapan bioteknologi dari bakteri yang resistan terhadap logam berat pada lingkungan laut yang tercemar maupun tidak tercemar. Kata kunci: resisten, Copper, Virgibacillus marismortui, MIC The microbial community associated with coral Goniastrea aspera from Panjang Island, Jepara waters was investigated using culture dependent molecular microbial techniques. The objectives on this study were to isolate, select and identify bacteria associated with coral Goniastrea aspera which resistant to heavy metal Copper (Cu). The tolerance patterns, expressed as MICs, for 17 coral bacteria to Cu heavy metal were surveyed by using an agar diffusion method. The most copper-resistant bacterium, GN10 isolat, was selected further to examine its molecular and physiological characteristics. Small-subunit rRNA gene-based analyses indicated that this bacterium was closely related to Virgibacillus marismortui strain 123, with a high homology of 99%. This bacterium may serve as bioassay indicator organisms and may be potential for biotechnological applications for metal-resistant bacteria in polluted and non-polluted marine environments. Key words: resistant, Copper, Virgibacillus marismortui, MIC

Potential Bacterial Symbion of Sea Urchin As a Multi-Drug Resistant (MDR) Antibacterial Agent Against Staphylococcus aureus and Escherichia Coli Bacteria Suzana Kristy Satriani Fofied; Agus Sabdono; Diah Permata Wijayanti
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 3 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Staphylococcus aureus and Escherichia coli are pathogenic bacteria agent of many human diseases. Those bacteria infect in various levels and also been antibiotic resistants. Bacterial resistance has become a serious global problem. The purposes of this study were to isolate and identify the symbiotic bacteria of the Sea Urchin that have an antibacterial activity of the Strain Multi-Drug Resistant (MDR) against Staphylococcus aureus and Escherichia coli. Sea Urchin was taken from Panjang island, Jepara Indonesia at 2-3 m depth. The symbiotic bacteria were isolated from Sea Urchin by using dilution method and spread plate method. Phenotypic characteristics was observed on colony shape, color and texture of growing bacteria. While the streak method was used to purify bacterial symbion. The antibacterial activity test was performed using overlay method. The results showed that 3 out of 37 isolates have antibacterial activity against Staphylococcus aureus and Escherichia coli. The BB.03.35 isolate was selected for molecular identification due to the largest inhibitory zone diameter. The sequence of this bacterium showed 97% homology and closely related to Pseudoalteromonas flavipulchra.

Ekotoksisitas Senyawa Cyanida pada Karang Porites lutea dan Galaxea fascicularis Irwani Irwani; Chrisna Adhi Suryono; Agus Sabdono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 2 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Salah satu cara metoda penangkapan ikan hias yang efektif adalah dengan melakuken pembiusan dengan menggunakan cyanida. Tujuan dari penelitian ini adalah untuk mengetahui toksisitas senyawa cyanida terhadap karang Porites lutea dan Galaxea fascicularis. Rancangan penelitian yang digunakan dalah split plot RAK dengan ulangan 3 kali jenis karang merupakan kelompok utama dan konsentrasi cyanide merupakan sub-kelompok. Pengamatan yang diamati adalah jumlah zooxanthellae dan proseptase kematian karang. Hasil penelitian menunjukan semakin tinggi konsentresi cyianida menunjukan semakin tinggi prosentese kematian karang. Semakin tinggi konsentrasi cyanida semakin kecil jumlah zooxanthellae pada karang.Kata kunci: cyanide, toksisitas, Porites luta dan Galaxea fascicularis One of the methods commonly used to capture ornamental fishes is by using cyanide. The purpose of this study was to investigate the toxicity of cyanide compound on coral Porites lutea and Galaxea fascicularis. The split plot randomized block design with 3 replicate was used in the present study. While the species of corals used as the main block and the cyanide concentration as the sub-block. The study was focused on the analyzed of the number of zooxanthellae and the percentage mortality of corals. The results of the study shows that increasing cyanide concentration increased the percentage mortality of coral and decreased the number of zooxanthellae on the coral.Keywords: cyanide, toxicity, Porites lutea and Galaxea fascicularis

Karakterisasi Molekuler Bakteri yang Berasosiasi dengan Penyakit BBD (Black Band Disease) pada Karang Acropora sp di Perairan Karimunjawa Agus Sabdono; Ocky Karna Radjasa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 3 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Black Band Disease (BBD) merupakan penyakit yang bersifat virulen terutama menyerang jenis karang batu.Meskipun komunitas bakteri BBD didominasi oleh jenis cyanobakterium, namun penelitian tentang jumlahkomposisi bakteri yang menyusun komunitas belum pernah dilakukan. Komunitas bakteri yang berasosiasi dengan penyakit BBD (black band disease) pada karang cabang Acropora sp. dalam penelitian ini diujidengan menggunakan teknik kultur dependent. Teknik molekuler gen 16S rDNA (amplifikasi 16S DNA ribosom) digunakan untuk karakterisasi komunitas secara komprehensif. Berdasarkan analisis sekuen gen 16SrDNA, data menunjukkan bahwa isolate BBD1 memiliki kekerabatan terdekat dengan Myroides odoratimimus (99.0%), isolat BBD2 adalah Bacillus algicola (99.6%) dan isolat BBD3 adalah Marine Alcaligenaceae bacteriumico (96.0%). Hasil identifikasi bakteri yang berasosiasi dengan penyakit BBD pada karang cabang Acropora sp di Karimunjawa merupakan komunitas baru yang berbeda dengan hasil penelitian terdahulu. Hasil inimemungkinkan untuk dilakukan penelitian lanjutan tentang isolasi dan kultur bakteri tersebut untuk bisa menerangkan etiologi penyakit.Kata kunci: Black Band Disease (BBD), Acropora sp., 16S rDNA.Black band disease (BBD) is a virulent disease primarily affecting scleractinian corals. Eventhough the BBD bacterial mat is dominated by a cyanobacterium, the quantitative composition of the BBD bacterial matcommunity has not described previously. The bacterial community associated with black band disease (BBD) of the branching corals Acropora sp. in this study was examined using culture-dependent techniques. Acomplementary molecular techniques of 16S rDNA genes [amplified 16S ribosomal DNA) was used to give a comprehensive characterization of the community. On the basis of the results of sequen analysis, our datashow that BBD1 isolate was closely related with Myroides odoratimimus (99.0%), BBD2 isolate was Bacillus algicola (99.6%) and BBD3 isolate was Marine Alcaligenaceae bacterium (96.0%). Of the three bacteria identified,these were not previously found in other studies. This result will allow the dominant BBD bacteria to be targeted for isolation and culturing experiments designed at interpreting the disease etiology.Key words: Black Band Disease, Acropora sp., 16S rDNA.

Kandungan Koprostanol dan Bakteri Coliform Pada Lingkungan Perairan Sungai, Muara, dan Pantai di Banjir Kanal Timur, Semarang Pada Monsun Timur Tri Yuni Atmojo; Tony Bachtiar; Ocky Karna Radjasa; Agus Sabdono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 1 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Limbah domestik merupakan salah satu sumber utama pencemaran di perairan pantai pada negara yang sedang berkembang yang masih kurang mendapatkan perhatian serius bila dibandingkan dengan pencemaran oleh industri. Namun dengan terus meningkatnya aktivitas manusia di wilayah pesisir dan kesadaran akan pentingnya lingkungan bersih bagi kesehatan, estetika dan alasan ekologis lainnya, deteksi tentang kontaminasi limbah menjadi penting untuk diketahui secara lebih baik. Selama ini indikator kontaminasi limbah domestik ditentukan berdasarkan jumlah mikroorganisme intestinal khususnya kelompok bakteri coliform. Koprostanol diusulkan sebagai alternatif indikator limbah domestik, sehingga diperlukan kajian eksistensi koprostanol untuk persyaratan kelayakannya sebagai indikator, serta bakteri coliform sebagai pembanding. Penelitian dilakukan pada bulan Juli-Agustus 2003 pada lingkungan sungai, muara dan pantai di sungai Banjir Kanal Timur di Semarang. Analisa coliform dari sampel air dan sedimen dilakukan di laboratorium Mikrobiogenetika, FMIPA UNDIP Semarang dan analisa konsentrasi koprostanol dilakukan di laboratorium Kimia dan FisikaPusat (LAKFIP) UGM Yogyakarta. Hasil menunjukkan bahwa koprostanol dapat terdeteksi pada sedimen dan tidak terdeteksi pada kolom air. Eksistensi koprostanol didapatkan nilai tertinggi pada lingkungan perairansungai (14,9 μg/g) dibandingkan muara (1,04 μg/g), dan pantai (5,25 μg/g). Bakteri total coliform terdeteksi pada kolom air maupun sedimen pada lingkungan perairan sungai (2,80 x 104 sel/100 ml), muara (0,4 x104 sel/100 ml), dan pantai (0 - 0,4 x 104) sel/100 ml, sementara fecal coliform terdeteksi di lingkungan perairan sungai (2 x 104 - 2,8 x 104 ) sel/100 ml, dan muara (0,4 x 104 sel/100 ml), namun tidak terdeteksipada lingkungan perairan pantai.Kata kunci : Koprostanol, limbah domestik, indikator pencemaran, coliformDomestic waste is one of the major sources of the pollution in coastal waters of most developing, countries, which has got less attention than industrial pollution. However, along with the increase of human activitiesin coastal areas coupled with the importance of clean environment for the health, esthetics, and ecological reasons, the detection of waste contamination has become important to be recognized. So far, the indicator of domestic waste contamination has been intestinal microorganism, especially coliform bacteria. Coprostanol is a proposed alternative indicator in the detected domestic waste those, it is definitely important to study the existence of coprostanol and coliform bacteria in order to fine its application. The research carried out from July to August 2003 at the environmental waters of river, estuarine, and coastal of Ciliwung, Jakarta; Banjir Kanal Timur, Semarang, analysis of coliform form water and sediment samples was conducted at micro biogenetic laboratory Faculty of Mathematic and Natural sciences, Diponegoro University, meanwhile analysis of coprostanil concentration was performed at central laboratory of chemistry and physic UGM. The results showed that coprostanol was detected in sediment but not in the water phase at all locations. The existence of coprostanol was detected either at environmental waters of river (14,9 μg/g) or estuarine (1,04 μg/g), and coastal (5,25 μg/g). Total coliform bacteria were detected both in water column and sediment of river (2,80 x 104 ) sel/100 ml, at estuarine (0,4 x 104) sel/100 ml and coastal (0 - 0,4 x104) sel/100 ml, meanwhile fecal coliform bacteria were detected in environmental water of river (2 x 104 - 2,8 x 104 ) sel/ 100 ml, and at estuarine (0 - 4 x 104) sel/100 ml, but not detected at coastal area. The use of coprostanol to considered for the indicator alternative because the existence can influence by various condition at water of river and used of coliform bacteria as domestic waste contamination indicator require to be considered because owning various weakness.Key words : Coprostanol, coliform, domestic waste, pollution indicator

Pengaruh Ekstrak Antifouling Bakteri Karang Pelagiobacter variabilis Strain USP3.37 terhadap Penempelan Barnakel di Perairan Pantai Teluk Awur, Jepara Agus Sabdono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 1 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Biofouling sebagai hasil dari proses penempelan organisme fouling pada berbagai struktur dilingkungan laut telah menimbulkan banyak kerugian bag! pelaku industri kelautan. Aplikasi cat pelindung antifoulant komersial yang komponen utamanya adalah logam berat seperti, TBT(tri-n-butyl tin), tembaga, telah berkembang menjadi masalah baru sehingga memerlukan cat pelindung yang ramah lingkungan. Bakteri yang berasosiasi dengan organisme di lingkungan laut diketahui menghasilkan metabolit sekunder sebagai sumber senyawa alternatif antifoulant. Bakteri Pelagiobacter variabilis UPS3.37 digunakan sebagai bahan ekstrak kasaryangdiformulasikan dengan cat untuk uji mikrofouling dan makrofouling di lapangan. Hasil penelitian menunjukkan bahwa ekstrak kasar P. variabilis UPS3.37 mempunyai aktifltas antifouling terhadap bakteri fouling. Pada uji makrofouling menunjukkan bahwa pemberian ekstrak kasar tanpa campuran cat mampu menurunkan jumlah penempelan bamakel. Teriihat adanya pola semakin tinggi konsentrasi ekstrak kasar semakin meningkatkan aktivitasantifouling. Berdasarkan karakter fenotip tersebut, bakteri P. variabilis UPS3.37 dapat digunakan sebagai organisme probiotik untuk antifouling di dalam menghilangkan penempelan bakteri pada biofilm.Kata kunci: antifouling, Pelagiobacter variabilis UPS3.37, barnakelMarine biofouling, despite a natural process as a result of organism growth on underwater surfaces that causeshuge economic losses to marine industries. Problems with heavy metal antifouling compounds, such as, TBT,copper have highlighted the need to develop new environmentally friendly antifouling coatings.Bacteria isolated from living surfaces in the marine environment are a promising source of natural antifoulingcompounds. Pelagiobacter variabilis UPS3.37 used to produce crude extract that was formulated with coatingpaints for microfouling and macrofouling assay in the field. The results showed activity against a test panel offouling bacteria. Further tested for their ability to inhibit the settlement of barnacle caused a decrease in thenumber of settled barnacles on crude extract without containing paint. The activity pattern showed that themore the crude extract, the higher the antifouling activity. This phenotype is important for the bacterium’suse as a probiotic organism for novel antifouling or removing bacteria attached in a biofilm.Key words : antifouling, Pelagiobacter variabilis UPS3.37, barnacle

Biodegradation of Polyethylene Microplastic using Culturable Coral-Associated Bacteria Isolated from Corals of Karimunjawa National Park Prastyo Abi Widyananto; Sakti Imam Muchlissin; Agus Sabdono; Bambang Yulianto; Fauziah Shahul Hamid; Ocky Karna Radjasa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 4 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.4.237-246

Polyethylene is a plastic material that was globally produced and is well known as a non-degradable pollutant product. Plastic pollution, primarily microplastics, have been distributed to coral reef ecosystems, where these areas are ecosystems with high productivity. Karimunjawa National Park in Indonesia is one of the protected areas for coral reef ecosystem habitat in Central Java, threatened by microplastic contamination. Recent studies have shown that coral-associated bacteria have an adequate ability to degrade marine pollutant materials. No one has reported that the use of indigenous coral-associated bacteria has the potential for microplastic biodegradation, especially low-density polyethylene microplastic materials. Hence, the objective of this study was to find the potential of microplastic biodegradation agents derived from coral-associated bacteria in Karimunjawa National Park area. Various coral life-forms were isolated in July 2020 from conservation areas and areas with anthropogenic influences. Bacterial isolates were screened using tributyrin and polycaprolactone as substrates to reveal potential microplastic degradation enzymes. The total isolation results obtained 92 bacterial isolates, and then from the result of enzyme screening, there were 7 active bacteria and only 1 bacteria that potential to degrade polyethylene. LBC 1 showed that strain could degrade by 2.25±0.0684 % low-density polyethylene microplastic pellet by incubating bacterial growth until the stationary phase. Identification of LBC 1 strain was carried out by extracting DNA and bacterial 16S rRNA sequences. Bacterial gene identification refers to Bacillus paramycoides with a similarity level in the National Center Biotechnology Information database of 99.44%. These results prove that hard coral association bacteria can degrade low-density polyethylene microplastics.

Physico-chemical Characteristics and Heavy Metal Contents in Shallow Groundwater of Semarang Coastal Region Chrisna A Suryono; Baskoro Rochaddi; Agus Sabdono; B Tyas Susanti
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 4 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Kandungan logam berat di dalam lingkungan perairan secara alami berasal dari hasil proses geokimia. Namun, kandungan logam berat dalam perairan ini dapat meningkat seiring dengan meningkatnya aktivitas manusia seperti, aktivitas pelayaran, limbah industri, limbah domesitk dan sebagainya. Dalam penelitian ini telah dilakukan studi untuk menentukan kandungan logam berat merkuri (Hg), timbal (Pb), besi (Fe), Kromium (Cr) dan tembaga (Cu). Parameter fisika-kimia contoh air dan kandungan logam berat sampel air yang berasal dari daerah pantai Semarang juga diukur dengan menggunakan Atomic Absorption Spectrophotometer (AAS). Hasil penelitian menunjukkan bahwa nilai keragaman antar parameter fisika kimia (pH, suhu, salinitas) adalah kecil. Warna, rasa dan bau dari sampel air juga diamati. Kandungan logam berat (mg L-1) dalam sampel air menunjukkan nilai Cr 5.083 + 1.59, Pb 5.52 + 1.34, Fe 1.199 + 1.29. Sedangkan logam Hg dan Cu dalam penelitian ini tidak terdeteksi. Nilai rerata hasil penelitian tersebut melebihi ambang maksimum yang disyaratkan World Health Organization (WHO) dan Indonesian Drinking & Domestic Water Quality Standard for Ground Water . Penelitian ini menunjukkan bukti adanya kontaminasi logam berat yang membahayakan pada suplai airtanah dangkal di daerah pantai Semarang. Kata kunci: parameter fisika kimia, logam berat, Atomic Absorption Spectrophotometer Heavy metals in the aquatic environment have to date come mainly from naturally occurring geochemical materials. However, this has been enhanced by human activities such as boat activity, industrial effluents, domestic sewage etc. An attempt was made to determine the level of trace metals such as Mercury (Hg), Lead (Pb), Iron (Fe), Chromium (Cr) and Copper (Cu). The physico-chemical and trace metal contents of water samples from coastal zone of Semarang were assessed using Atomic Absorption Spectrophotometer technique. Results indicated that low variation existed among some physico-chemical parameter (pH, temperature, salinity). In the water sample colour, taste and odor were investigated. Heavy metal levels (mg L-1) in the water were Cr 5.083 + 1.59, Pb 5.52 + 1.34, Fe 1.199 + 1.29. However, Hg and Cu were not detected in any of the samples. Comparison of the metal contents in the water sample with World Health Organization (WHO) limits and Indonesian Drinking & Domestic Water Quality Standard for Ground Water showed that the mean levels of Fe, Pb, Cr were exceeded the maximum permissible levels for drinking water. This work has conclusively proven the presence of dangerous heavy metal contamination of the groundwater supply in the coastal area of Semarang. Key words: Physico-chemical parameters, heavy metals, Atomic Absorption Spectrophotometer

Pertumbuhan Karang Bercabang Acropora aspera, Stylophora pistilata dan Pocillopora darmicornis Hasil Planulasi di Laboratorium Diah P Wijayanti; Chrisna A Suryono; Agus Sabdono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 2 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini berlangsung di perairan Pulau Panjang Jepara pada tahun 2003. Tujuan dari penelitian ini adalah untuk mengetahui perkembangan planula yang ditransplan di perairan. Metoda yang digunakan adalahdiskriptip dengan pengamatan langsung dan menginterprestasikannya. Hasil yang didapat menunjukan pola perkembangan planula tumbuh menjadi karang muda. Pertumbuhan paling cepat dialami oleh A asperanamun ketiga jenis karang tidak menunjukan perbedaan secara nyata dalam pertumbuhan. Selama pengamatan kondisi perairan sangat sesuai untuk tumbuhnya karang.Kata kunci : Planula, A. aspera, S, pistilata, P. darmicornisThe research was held at Panjang Island waters in 2003. The aim of the study was understanding the development of planula which were transplanted in the sea. Descriptive method with direct watching andinterpretation was used in this study. The result showed that planula growth to be young coral reef. The faster growth was shown by A. aspera, but all the coral was not significant different in growth. During theresearch water quality condition was suitable for coral to grow.Key words: Planula, A. aspera, S, pistilata, P. darmicornis

Bakteri Simbion Karang Porites dari Perairan Gunungkidul, Yogyakarta dan Aktivitas Antibakteri terhadap Bakteri Patogen Staphylococcus aureus dan Escherichia coli Rivan Novianto Madilana; Diah Permata Wijayanti; Agus Sabdono
Buletin Oseanografi Marina Vol 7, No 1 (2018): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Porites merupakan genus karang yang memiliki persebaran luas di Indonesia, termasuk perairan Gunungkidul, Yogyakarta. Penelitian menunjukkan bahwa bakteri simbion karang Porites memiliki potensi antibakteri dalam menanggulangi bakteri patogen. Penelitian ini betujuan untuk mengetahui jenis bakteri simbion karang Porites dari Perairan Gunungkidul Yogyakarta yang memiliki aktivitas antibakteri patogen S. aureus dan E. coli. Bakteri simbion diisolasi dari fragmen jaringan karang dengan pengenceran bertingkat, kemudian uji aktivitas antibakteri dilakukan dengan menggunakan metode overlay dan difusi paperdisk. Delapan dari 64 isolat aktif menghambat kedua bakteri patogen Staphylococcus aureus dan Escherichia coli. Dua diantaranya merupakan isolat unggul yang menunjukkan aktivitas antibakteri paling baik. Kedua isolat selanjutnya diidentifikasi karakteristik molekular DNA dengan sekuen gen 16S rRNA. Hasil identifikasi 16S rRNA menunjukkan isolat GKP1.4.3 memiliki kesamaan 99% dengan Bacillus pumilus strain NBRC 12092, dan isolat GKP3.2.2 memiliki kesamaan 99% dengan Vibrio natriegens strain NBRC 15636.Porites is a coral which has distributed widely in Indonesia, including Gunungkidul Waters, Yogyakarta. Research has shown that Porites coral symbiont bacteria have antibacterial potency against pathogenic bacteria.This study aims to determine the type of Porites coral symbiont bacteria collected from the waters of Gunungkidul Yogyakarta which has antibacterial activity of S. aureus and E. coli. Bacteria symbionts were isolated from coral tissue fragments by serial dillution method, while antibacterial activity was performed by using overlay and paperdisk diffusion method. Eight of the 64 active isolates inhibited both pathogenic bacteria S. aureus and E. coli. Two of 8 isolates showed stronger antibacterial activity. The two isolates subsequently identified the molecular characteristics of DNA with the 16S rRNA gene sequence. The identification of 16S rRNA showed that GKP1.4.3 isolate had 99% similarity with Bacillus pumilus strain NBRC 12092, and GKP3.2.2 isolate had 99% similarity with Vibrio natriegens strain NBRC 15636.

Co-Authors Agus Indarjo Agus Trianto Agus Trianto Agus Trianto Agus Triyanto Agustina Agustina Agustina Aiyen Tjoa Aldion Adin Nugroho Ali Djunaedi Ali Djunaedi Ali Ridlo Ambariyanto , Ambariyanto Ambariyanto Angelina Ferawaty Siregar Angelina Ferawaty Siregar Aninditia Sabdaningsih Azizi, Muhammad Faris B Tyas Susanti B. Tyas Susanti Bambang Yulianto Baskoro Rochaddi Bhaskoro Rochaddi Burhan Habibi Yunus Chrisna A Suryono Chrisna A Suryono Chrisna A Suryono Chrisna Adhi Suryono Chrisna Adhi Suryono Delianis Pringgenies Denny Nugroho Sugianto Desy Wulan Triningsih DIAH AYUNINGRUM Diah Ayuningrum, Diah Diah P Wijayanti Diah P Wijayanti Diah Permata Wijayanti Diah Permata Wijayanti Diah Permata Wijayanti Dio Dirgantara Duhita Sinidhikaraning Kencana Elena Zocchi Endang Sri Lestari Erwin Ivan Riyanto Erwin Ivan Riyanto Eunike Dorothea Hutapea Farrastasya Muflihul Azzami Fauziah Shahul Hamid Gina Saptiani Gina Saptiani Hadi Endrawati Haeruddin Haeruddin Hakim, Muhamad Fikri Hudi Nur Hans Arthur Philips HANS- PETER GROSSART Hans-Peter Grossart Herawati Sudoyo Herida, Azalia Puspa Hermin Pancasakti Kusumaningrum Heru Kurniawan Alamsyah Ibnu Pratikto Ida Ayu Putu Sri Widnyani Intan Budi Setiasih Irwani Irwani Irwani Irwani Irwani Irwani Irwanto, Eko Ita Widowati JOEDORO SOEDARSONO Johannes F Imhoff Jusup Suprijanto Jusup Suprijanto Jutta Wiese Larasati, Stefanie Jessica Henny Leenawaty Limantara Mada Triandala Sibero Mada Triandala Sibero Meinhard Simon Miftahuddin Majid Khoeri Misbakul Munir Muchlissin, Sakti Imam Muhamad Fikri Hudi Nur Hakim Muhamad Ziaul Faiz Muhammad Eka Darmawan Rafsanjani Muhammad Zainuri Nadya Cakasana Norma Afiati Norma Afiati Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Ocky Karna Radjasa Ocky Karna Radjasa ocky radjasa Popi IL Ayer Prastyo Abi Widyananto Puspa Kapinangasih Putut Har Riyadi Raden Ario Rafsanjani, Muhammad Eka Darmawan Ragil Saptaningtyas Raja Aditya Sahala Siagian Ratna Diyah Palupi Retno Hartati Rignolda Djamaludin Rina Setyowati Sulistiyoningrum Rini Pramesti Rivan Novianto Madilana Romadhon Romadhon Rory Anthony Hutagalung Rosa Amalia Rudhi Pribadi Rudiger Stöhr S Sukarmi S. Sulistiyani Sakti Imam Muchlissin Sakti Imam Muchlissin Sakti Imam Muchlissin Setiasih, Intan Budi Setyani, Wilis Ari Sibero, Mada Triandala Slamet Budi Prayitno Sri Achadi Nugraheni Sri Lintang Artono Sri Redjeki Sri Redjeki Stalis Norma Ethica Stefanie Jessica Henny Larasati Subagiyo Subagiyo Subagiyo Subagiyo Subagyo Subagyo Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sunaryo Sunaryo Suzana Kristy Satriani Fofied Syauqina Nashihi Aufar Thorsten Brinkoff TONNY BACHTIAR Tonny Bachtiar Tony Bachtiar Tony Bachtiar Torben Marten TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Ulfah Amalia Wahyuningsih, Candra Widy Febriansyah Wilis Ari Setyani Yeni Sulistiyani Yesaya Putra Pamungkas

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