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All Journal HAYATI Journal of Biosciences PROSIDING SEMINAR NASIONAL JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia Jurnal Matematika & Sains Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marine Research in Indonesia Jurnal Segara ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Jurnal Perikanan

Agus Sabdono

Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro

Author-ID : 367511

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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EKSISTENSI KOPROSTANOL DAN BAKTERI COLIFORM PADA LINGKUNGAN PERAIRAN SUNGAI, MUARA, DAN PANTAI DI JEPARA PADA MONSUN TIMUR Tri Yuni Atmojo; Tony Bachtiar; Ocky Karna Radjasa; Agus Sabdono
Jurnal Ilmu Lingkungan Vol 9, No 1 (2011): April 2011
Publisher : School of Postgraduate Studies, Diponegoro Univer

Limbah domestik merupakan salah satu sumber utama pencemaran di perairan pantai pada negara yang sedang berkembang, namun kurang mendapatkan perhatian serius dibandingkan dengan pencemaran oleh industri. Dengan terus meningkatnya aktivitas manusia di wilayah pesisir dan kesadaran akan pentingnya lingkungan bersih bagi kesehatan, estetika dan alasan ekologis lainnya, deteksi tentang kontaminasi limbah menjadi penting untuk diketahui secara lebih baik. Selama ini indikator kontaminasi limbah domestik ditentukan berdasarkan jumlah mikroorganisme intestinal khususnya kelompok bakteri coliform. Koprostanol diusulkan sebagai alternatif indikator limbah domestik, sehingga diperlukan kajian eksistensi koprostanol untuk persyaratan kelayakannya sebagai indikator, serta bakteri coliform sebagai pembanding.Penelitian dilakukan lingkungan sungai, muara dan pantai di sungai Ciliwung Jakarta. Hasil menunjukkan bahwa koprostanol dapat terdeteksi pada sedimen dan tidak terdeteksi pada kolom air. Eksistensi koprostanol didapatkan nilai tertinggi pada lingkungan perairan sungai (5,81μg/g) dibandingkan muara (5,63μg/g), dan pantai (2,93μg/g). Bakteri total coliform terdeteksi pada kolom air maupun sedimen pada lingkungan perairan sungai (2,8 x 104 - 4,3 x 104) sel/100 ml, muara (0 - 4 x 103) sel/100 ml, dan tidak terdeteksi pada lingkungan perairan pantai, sementara fecal coliform terdeteksi di lingkungan perairan sungai (2 x 104 - 4,3 x 104 ) sel/100 ml, dan muara (0 - 4 x 104 sel/100 ml), namun tidak terdeteksi pada lingkungan perairan pantai.

AKTIVITAS JAMUR SIMBION SPONS TERHADAP JAMUR Trichophyton sp. DI PULAU BIAK, KABUPATEN BIAK-NUMFOR, PAPUA Popi IL Ayer; Agus Sabdono; Agus Trianto
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 1, No 1 (2018)
Publisher : ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua

Jamur Trichophyton sp. merupakan penyebab penyakit kulit yang disebut dengan Tinea Imbrikata. Persebaran penyakit ini di Indonesia dapat ditemukan di wilayah tertentu antara lain Papua, Sulawesi dan Sumatra. Kasus Tinea imbrikata banyak ditemukan di bagian Selatan Samudra Pasifik dengan tingkat infeksi ditemukan sebesar 18% di Papua dan Papua New Guinea. Organisme laut memiliki potensi yang sangat besar dalam menghasilkan senyawa-senyawa aktif yang dapat digunakan sebagai bahan baku obat-obatan dibandingkan dengan senyawa yang dihasilkan oleh tumbuhan teresterial. Spons merupakan invertebrata laut berpori yang bersifat filter feeder sehingga menjadi habitat bagi mikroorganisme. Jamur merupakan mikroorganisme simbion spons selain bakteri yang berpotensi sebagai sumber senyawa metabolit sekunder dalam bidang farmakologi. Tujuan dari penelitian ini adalah untuk mengetahui jenis-jenis jamur simbion spons C1K1 yang memiliki aktivitas antijamur terhadap jamur Trichophyton sp. Isolasi jamur simbion spons dilakukan dengan menggunakan metode pengenceran dan metode tempel. Identifikasi isolat jamur dilakukan dengan pengamatan morfologi koloni jamur yang terbentuk, kemudian dilakukan pemisahan isolat satu dengan lainnya. Hasil isolasi menunjukan terdapat 25 isolat jamur simbion spons (C1K1). Analisis pengujian antijamur tahap awal dilakukan dengan metode overlay dimana diperoleh 4 isolat jamur yang memiliki aktivitas antijamur terhadap jamur Trichophyton sp.Kata kunci: Trichophyton sp., jamur simbion spons, metabolit sekunder

Antipathogenic Activity of Acroporid Bacterial Symbionts Against Brown Band Disease-Associated Bacteria Rosa Amalia; Diah Ayuningrum; Agus Sabdono; Ocky Karna Radjasa
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 2 (2021): August 2021
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.536

The coral reefs’ condition in most regions in Indonesia has been declining due to coral diseases, such as Brown Band Disease (BrBD). A treatment for BrBD involves the use of biological control agents that have antagonistic properties against disease-causing agents. This study aimed to isolate bacteria from healthy hard coral, those associated with BrBD, and those that had bioactivities against BrBD. Sampling and identification of corals and BrBD were carried out in March 2015 at the Marine National Park of Karimunjawa. Bacteria from healthy and infected corals were isolated and purified. The isolates were subjected to antipathogenic assay using overlay and agar diffusion methods. Finally, molecular identification of active bacteria was carried out using the 16S rRNA gene amplification. As many as 57 bacterial isolates were obtained from healthy coral, as well as four bacterial isolates from coral with BrBD symptoms. A total of 15 bacterial isolates (26%) showed antipathogenic activity against BrBD-associated bacteria. Three isolates with the strongest antipathogenic activities, i.e., GAMSH 3, KASH 6, and TAPSH 1 were identified by 16S rRNA gene sequences. The results showed that they were aligned to Virgibacillus marismortui (97%), Oceanobacillus iheyensis (97%), and Bacillus cereus (96%), respectively.

Coral Recruitment on Artificial Patch Reefs Deployed in The Marginal Reefs: Effect of Multilevel Substrate on Density of Coral Recruit Munasik Munasik; Agus Sabdono; Eunike Dorothea Hutapea; Sugiyanto Sugiyanto; Denny Nugroho Sugianto
Jurnal Segara Vol 17, No 1 (2021): April
Publisher : Politeknik Kelautan dan Perikanan Dumai

A study of coral recruitment on Artificial Patch Reefs (APR) was performed in the marginal reef of Panjang Island, Central Java (Java Sea) to examine whether multilevel substrates of APR affect the density of coral recruits. Long-term and short-term observations were applied in yearly monitoring 2017-2019 and biweekly observations for 3 months in early 2019. Coral recruitment significantly varied among level substrates of APR (F(a,b) .05=3.08; p-value<0.05) and there was a significant difference at the beginning of the year (F(a,b) .05=5.52; p-value<0.05). The density of recruits on the substrates after 4 years post-deployment of APRs was 0.2 to 129.2 m-2 while the recruitment rate within short-term observations was 0.28-1.28 m-2 per month. The highest coral recruitment occurred at the middle to the top level of APR, while the lowest recruitment was found in the lowest level of APR. This is possibly due to high resuspension from the seabed. Oulastrea was dominant in both long- and short-term recruitment periods while Pocillopora was rare due to post-settlement mortality which trigger the overgrown coral-killing sponges. The results indicated that the adaptation of coral recruitment in the marginal environment is determined by the high recruitment of the small-colony coral species which possessed stress-tolerant for turbidity disturbance, such as Oulastrea crispata. This study suggested that the multilevel substrates, Artificial Patch Reefs (APR) are one of the reef rehabilitation methods which can be applied in the marginal environment enhancing coral recruitment.

Penapisan Aktivitas Antibakteri Dari Bakteri Asosiasi Spons Terhadap MRSA (Methicilin-resistant Staphylococcus aureus) Farrastasya Muflihul Azzami; Agus Trianto; Agus Sabdono
Journal of Marine Research Vol 11, No 2 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i2.31813

Resistensi bakteri patogen tehadap antibiotik menjadi masalah besar bagi dunia kesehatan. Bakteri Staphylococcus aureus yang resisten terhadap antibiotik yang dikenal dengan MRSA (Methicilin-resistant Staphylococcus aureus). Bakteri MRSA mengalami resistensi antibiotik disebabkan oleh mutasi genetik yang disebabkan terapi antibiotik yang bersifat irasional. Penyakit yang disebabkan oleh bakteri patogen MRSA dengan tingkat infeksi yang bervariasi dapat terjadi pada jaringan lunak, tulang, organ pernapasan, serta jaringan endovaskuler yang menimbulkan indikasi berbagai penyakit seperti bronkitis, pneumonia, meningitis, dan sepsis. Masalah resistensi antibiotik dapat diatasi dengan pencarian antibiotik baru dengan eksplorasi bahan alam yang berasal dari laut seperti spons. Spons menyediakan tempat hidup utk bakteri simbion dan simbion membantu spons dlm proses nutrifikasi spons menghasilkan senyawa metabolit untuk perlindungan diri dari pathogen dan prodatornya, kompetisi ekologis. Metabolit yang dihasilkan oleh spons merupakan hasil biosintesis sehingga bakteri asosiasinya memiliki komponen yang mirip dengan spons. Tujuan penelitian untuk mencari bakteri yang berasosiasi dengan spons yang memiliki aktivitas antibakteri terhadap bakteri patogen MRSA dan mengidentifikasi secara molekuler bakteri asosiasi spons tersebut. Penelitian ini menggunakan metode eksperimental laboratoris dengan melakukan pengujian aktivitas antibakteri dari bakteri yang berasosiasi dengan spons. Metode yang dilakukan yaitu purifikasi bakteri, penapisan aktivitas antibakteri dengan metode agar plug dan identifikasi molekuler isolat bakteri. Hasil dari penelitian ini didapatkan 2 isolat yang berasosiasi dengan spons yang memiliki aktivitas antibakteri terhadap MRSA yaitu isolat RA-S20.4 dan RA-S20.5. Zona hambat isolat RA-S20.4 sebesar 8,3 mm dan isolat RA-S20.5 sebesar 9,6 mm. Hasil identifikasi isolat bakteri secara molekuker didapatkan bahwa isolat RA-S20.4 serta RA-S20.5 memiliki kekerabatan dekat dengan Vibrio alginolyticus sebesar 99,86% dan 99,65%. Pathogen bacteria resistance towards antibiotics have been a major problem in the medical field. The bacteria Staphylococcus aureus is known to be resistant to antibiotics known as MRSA. MRSA bacteria obtained their ability to be resistant towards antibiotics through gene mutations caused by irrational antibiotic application. MRSA are capable of causing diseases with various diseases such as bronchitis, pneumonia, meningitis, and sepsis. Antibiotic resistance can be overcome by discovering new antibiotics through exploration of marine natural products such as sponges. Marine sponges are hosts for various microbes such as bacteria, because they can protect microbes from predators by producing secondary metabolite. The metabolites that are produced by sponges are the result of biosynthesis, in which the bacteria that associated with these sponges have compounds similar to those of sponges. The purpose of this research is to discover bacteria associated with sponges that have antibacterial activity against MRSA pathogen bacteria, as well as to molecularly identify the sponge-associated bacteria. The activity in this study is bacterial isolation and purification, antibacterial screening activity, and molecular identification of bacterial isolates. Result shows that there are 2 isolates associated with sponges that have antibacterial activity against MRSA, namely isolate RA-S20.4 and RA-S20.5. The inhibition zone for isolate RA-S20.4 was 8,3 mm and isolate RA-S20.5 was 9,6 mm. The result of molecular identification of bacterial isolates shows that RA-S20.4 and RA-S20.5 were closely related to Vibrio alginolyticus by 99.86% and 99.65%.

Molecular Identification of Brown Algae Sargassum sp. from the Lombok Coastal Waters Yeni Sulistiyani; Norma Afiati; Haeruddin Haeruddin; Agus Sabdono
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.14760

Sargassum is a well-known genus of brown algae in Indonesia that has long been investigated due to its economic importance. To support its biological research, it is important to identify and classify the species studied. Morphological identification had greatly contributed to taxonomy, however, it cannot distinguish Sargassum species accurately due to its plasticity. The current study aims to identify Lombok Sargassum molecularly using an ITS2 DNA barcode. Fresh algae were collected from Ekas Bay (EB) and Aan Cape (AC) in Lombok. DNA was first purified, and then its gene product was amplified using ITS2 primers. DNA sequences were examined and traced using the Basic Local Alignment Search Tool (BLAST). DNA sequences were processed in MEGA-X to reconstruct the phylogenetic tree and estimate the genetic distance. Three species were identified based on the BLAST results: Sargassum cf granuliferum, Sargassum polycystum, and Sargassum oligocystum. The base length obtained ranged from 521 to 637 bp, with a similarity percentage of 99.25 to 100%. The phylogenetic tree exhibited each recognized Sargassum species was clustered with the same species from the gene bank. Interspecies genetic distance was 0,000-0,0039, while amongst Sargassum species it's 0,0136-0,2395. The genetic distance between Sargassum and Ulva adherens (outgroup) was >1. Sargassum species found in Lombok were closely related to other similar species in the GenBank.

Analisis Morfometri Rajungan (Portunus pelagicus) di Perairan Desa Keboromo Kabupaten Pati Jawa Tengah Hans Arthur Philips; Sri Redjeki; Agus Sabdono
Journal of Marine Research Vol 11, No 3 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i3.33340

Rajungan merupakan hasil laut bernilai ekonomi tinggi yang telah lama dikonsumsi oleh masyarakat dalam dan luar negeri. Tingginya nilai jual yang terdapat di pasar luar dan dalam negeri menyebabkan adanya peningkatan terhadap jumlah penangkapan rajungan. Upaya penangkapan yang terus meningkat menyebabkan hasil tangkapan nelayan semakin lama semakin menurun. Penelitian ini bertujuan untuk mengetahui variasi lebar karapas, berat tubuh, pola pertumbuhan, rasio jenis kelamin dan persentase tingkat kematangan gonad, hubungan lebar karapas dan berat tubuh dan siklus reproduksi rajungan di Desa Keboromo. Penelitian ini menggunakan metode deskriptif. Hasil dari penelitian ini menunjukkan dari 977 ekor rajungan yang diamati, sebaran ukuran lebar karapas rajungan rajungan jantan dan betina yang tertangkap selama penelitian berkisar antara 50-169 mm dengan nilai total tertinggi berada pada interval 100-109 mm dengan jumlah 358 ekor. Hubungan lebar karapas dan berat rajungan menghasilkan pola pertumbuhan alometrik negatif dengan nilai b sebesar 1,47 pada rajungan jantan dan 1,44 pada rajungan betina. Jumlah rasio kelamin rajungan jantan yang tertangkap di Desa Keboromo sebesar 57% (554 ekor) dan 43% (423 ekor) pada rajungan betina. Tingkat kematangan gonad pada rajungan betina terbanyak berada pada kelas II dengan persentase 77% dan dengan jumlah 325 ekor dengan ciri-ciri ukuran abdomen lebar, berwarna gelap (tampak luar abdomen), sudah berisi gonad dan terkadang berwarna jingga. Crab is a marine product with high economic value that has long been consumed by people at home and abroad. The high selling value found in foreign and domestic markets causes an increase in the number of crab catching. The increasing fishing effort causes fishermen's catches to decrease over time. This study aims to determine the variation of carapace width, body weight, growth pattern, sex ratio and the percentage of gonadal maturity level, the relationship between carapace width and body weight and the reproduction cycle of crabs in Keboromo Village. This research uses a descriptive method. The results of this study showed that from 977 crabs observed, the distribution of carapace width of male and female crabs caught during the study ranged from 50-169 mm with the highest total value being at intervals of 100-109 mm with a total of 358 individuals. The relationship between carapace width and crab weight resulted in a negative allometric growth pattern with a b value of 1.47 for male crabs and 1.44 for female crabs. The sex ratio of male crabs caught in Keboromo Village was 57% (554) and 43% (423) for female crabs. The highest level of gonad maturity in female crabs was in class II with a percentage of 77% and with a total of 325 tails with the characteristics of a wide abdomen, dark color (outside of the abdomen), filled with gonads and sometimes orange.

Tingkah Laku dan Kemunculan Hiu Paus (Rhincodon typus, Smith 1828) di Pantai Bentar Probolinggo Puspa Kapinangasih; Diah Permata Wijayanti; Agus Sabdono
Journal of Marine Research Vol 11, No 1 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i1.31727

Hiu Paus diketahui muncul di Pantai Bentar dari bulan Desember hingga Maret. Kemunculan Hiu Paus diduga berhubungn dengan aktivitas makan. Fenomena ini dimanfaatkan oleh masyarakat sekitar Pantai Bentar untuk menarik kedatangan wisatawan. Kondisi tersebut berpotensi menimbulkan dampak negatif bagi Hiu Paus. Penelitian ini bertujuan untuk mengetahui lokasi kemunculan Hiu Paus di Pantai Bentar serta perubahan tingkah laku Hiu Paus saat kegiatan wisata berlangsung. Penelitian dilakukan pada bulan Maret 2021 menggunakan metode observasi langsung dengan melakukan pengamatan di lokasi yang diduga menjadi tempat kemunculan Hiu Paus. Data tambahan diperoleh dari hasil wawancara dengan pengelola dan pemandu wisata. Lokasi kemunculan dicatat menggunakan GPS. Total lokasi kemunculan yang ditemukan berjumlah 9 lokasi yang tersebar di Perairan Gending, yaitu di bagian barat dan timur Pantai Bentar. Kemunculan terbanyak berada di timur Pantai Bentar. Hal ini karena wilayah tersebut dekat dengan bagan dan menjadi wilayah pelayaran nelayan Ikan Teri yang merupakan makanan Hiu Paus. Hiu Paus yang ditemukan berjumlah 9 ekor. Tingkah laku Hiu Paus yang ditemukan ketika aktivitas wisata berlangsung, yaitu perubahan secara perlahan seperti menyelam dan mendekat secara perlahan, perubahan mendadak seperti menjauh secara mendadak, perubahan secara tajam seperti menjauh secara tajam dan tidak merespon. Faktor yang mempengaruhi perubahan tingkah laku diduga karena adanya aktivitas yang dilakukan oleh manusia seperti berenang, mengejar Hiu Paus, melihat dari kapal, pelayaran kapal dan pemberian pakan. Hasil dari penelitian ini diharapkan dapat memberi masukan dalam pengelolaan wisata Hiu Paus Pantai Bentar. Whale sharks are known to appear at Bentar Beach from December to March. The appearance of whale sharks is thought to be related to feeding activities. This phenomenon is used by the community around Bentar Beach to attract tourist arrivals. This condition has the potential to have a negative impact on the whale sharks. This study aims to determine the location of the appearance of whale sharks on Bentar Beach and changes in whale shark behavior during tourism activities. The study was conducted in March 2021 using the direct observation method by making observations at the location suspected to be the place where the Whale Shark appeared. Additional data obtained from interviews with managers and tour guides. The location of occurrence is recorded using GPS. The total location of the emergence found was 9 locations scattered in Gending Waters, namely in the west and east of Bentar Beach. Most occurrences are in the east of Bentar Beach. This is because the area is close to Bagan and is a shipping area for Anchovy fishermen which are the food for whale sharks. There were 9 whale sharks observed. Whale Shark behavior found during tourist activities, namely slow changes such as diving and approaching slowly, sudden changes such as moving away suddenly, sharp changes such as moving away sharply and not responding. Factors that influence behavior change are thought to be due to activities carried out by humans such as swimming, chasing whale sharks, watching from ships, ship sailing and feeding. The results of this study are expected to provide input in the management of Whale Shark tourism in Bentar Beach.

Nutrient Enrichment Impact of Wastewater Shrimp Ponds on Coral Reefs of Nyamplungan Village, Karimunjawa Raja Aditya Sahala Siagian; Agus Sabdono; Sunaryo Sunaryo; Agus Trianto; Dio Dirgantara
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 3 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.3.267-278

Increased nutrients can cause a rise prevalence in coral disease. Shrimp pond wastewater can produce nitrate, phosphate, and ammonia from the residual of the feeding and shrimp feces. Nyamplungan village, Karimunjawa was selected as the study site as it represents a location with shrimp pond activity that close to coral reef ecosystem. This study aims to examine the difference of disease prevalence, coral cover, and coral genus diversity at ±300 m and ±1000 m from the shrimp pond and to examine the relationships between disease prevalence and nutrients (nitrate, phosphate, ammonia). Coral disease prevalence was measured within a modified 30 x 2 m belt transect at six sampling stations. Station 1, 2, and 3 were 6-8 m in depth (±1000 m from the shrimp pond), then station 4, 5, and 6 were depth 1-3 m (±300 m from the shrimp pond). Coral genus diversity was conducted by using the Roving Diver Visual Method. Coral cover was measured within Line Intercept Transect (LIT) 30 m. The results show that station 5 had the highest disease prevalence (30.80%±2.78%). Station 1 had highest coral cover (90.87%±7.16%). Coral genus diversity had a medium category for each station. Disease prevalence was significantly different between station depths of 1-3 m (±300 m to the shrimp pond) and depths 6-8 m (±1000 m from the shrimp ponds) (P<0.05). Disease prevalence had strong relationship with nitrate (r= 0.975), phosphate (r= 0.972) and ammonia (r= 0.958). These results suggest that the continuation of coral monitoring with additional disease incidence and disease progression, temporally.

Degradasi Karbohidrat pada Pakan Udang oleh Isolat Kapang Endofit Mangrove Sri Lintang Artono; Agus Trianto; Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya; Agus Sabdono; Subagiyo Subagiyo; Delianis Pringgenies; Rignolda Djamaludin; Aiyen Tjoa
Jurnal Kelautan Tropis Vol 26, No 1 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i1.17292

Feed quality is a very important factor in shrimp farming because it will affect shrimp growth, water quality and even the emergence of pathogenic bacteria. High quality feed can be well digested by shrimp so that it can improve the growth and do not leave plenty of residue. Mold is capable of producing cellulase which can be used to improve feed quality by shortening carbohydrate chains. This paper will discuss the application of endophytic molds to simplify cellulose in shrimp feed. Mold isolates were collected from mangrove ecosystems in North Sulawesi. The media used for culturing mold are potato dextrose agar (PDA) and potato dextrose broth (PDB). Fermentation test was conducted using the fungal isolates; Hypocreales sp. and Diaporthe stewartii and consortia and controls. Fermentation results were checked by TLC and Fehling's test to determine the composition of the compounds and the presence of reducing sugar either mono or di-saccharide. Fermented shrimp feed shows differences in texture and color. Fermented feed extract with Hypocreales sp. 2.69 g (3.36%), Diaporthe stewartii 4.9 g (6.13%), and consortium 3.75 g (4.69%). TLC results neither under UV light nor visualization of vanillin sulfate did not show any differences of compounds in the control and the fermented feed. The results of the Fehling test showed that the mold was able to degrade cellulose that can be utilized to increase the shirmp feed quality. Kualitas pakan merupakan faktor yang sangat berpengaruh dalam budidaya udang karena akan mempengaruhi pertyumbuhan udang, kualitas air bahkan timbulnya bakteri pathogen. Pakan yang baik mudah dicerna oleh udang sehingga dapat meningkatkan pertumbuhan secara maksimal dan tidak terlalu banyak meninggalkan residu. Kapang mampu memproduksi selulase yang dapat digunakan untuk meningkatkan kualitas pakan dengan memperpendek rantai karbohidrat. Tulisan ini akan membahas aplikasi kapang endofit untuk menyederhanakan selulosa pada pakan udang. Isolat kapang dikoleksi dari ekosistem mangrove di Sulaweri Utara. Media yang digunakan untuk mengkultur kapang adalah potato dextrose agar (PDA) dan potato dextrose broth (PDB). Uji fermentasi dengan kapang Hypocreales sp. dan Diaporthe stewartii dan konsorsium serta kontrol. Hasil fermentasi dicek dengan TLC dan uji Fehling untuk mengetahui komposisi senyawa dan keberadaan mono-karbohidrat. Pakan udang yang difermentasi menunjukkan perbedaan tekstur dan warna. Ekstrak pakan fermentasi dengan Hypocreales sp. sebesar 2,69 g (3,36%), Diaporthe stewartii sebesar 4,9 g (6,13%), dan konsorsium sebesar 3,75 g (4,69%). Hasil TLC dan visualisasi vanillin sulfat tidak menunjukkan perbedaan jenis senyawa pada pakan kontrol dan pakan terfermentasi. Hasil uji Fehling menunjukkan bahwa kapang mampu mendegradasi selulosa yang dapat digunakan untuk meningkatkan kualitas pakan udang.

Co-Authors Agus Indarjo Agus Trianto Agus Trianto Agus Trianto Agus Triyanto Agustina Agustina Agustina Aiyen Tjoa Aldion Adin Nugroho Ali Djunaedi Ali Djunaedi Ali Ridlo Ambariyanto , Ambariyanto Ambariyanto Angelina Ferawaty Siregar Angelina Ferawaty Siregar Aninditia Sabdaningsih Azizi, Muhammad Faris B Tyas Susanti B. Tyas Susanti Bambang Yulianto Baskoro Rochaddi Bhaskoro Rochaddi Burhan Habibi Yunus Chrisna A Suryono Chrisna A Suryono Chrisna A Suryono Chrisna Adhi Suryono Chrisna Adhi Suryono Delianis Pringgenies Denny Nugroho Sugianto Desy Wulan Triningsih DIAH AYUNINGRUM Diah Ayuningrum, Diah Diah P Wijayanti Diah P Wijayanti Diah Permata Wijayanti Diah Permata Wijayanti Diah Permata Wijayanti Dio Dirgantara Duhita Sinidhikaraning Kencana Eko Irwanto Elena Zocchi Endang Sri Lestari Erwin Ivan Riyanto Erwin Ivan Riyanto Eunike Dorothea Hutapea Farrastasya Muflihul Azzami Fauziah Shahul Hamid Gina Saptiani Gina Saptiani Hadi Endrawati Haeruddin Haeruddin Hakim, Muhamad Fikri Hudi Nur Hans Arthur Philips HANS- PETER GROSSART Hans-Peter Grossart Herawati Sudoyo Herida, Azalia Puspa Hermin Pancasakti Kusumaningrum Heru Kurniawan Alamsyah Ibnu Pratikto Ida Ayu Putu Sri Widnyani Intan Budi Setiasih Irwani Irwani Irwani Irwani Irwani Irwani Ita Widowati JOEDORO SOEDARSONO Johannes F Imhoff Jusup Suprijanto Jusup Suprijanto Jutta Wiese Larasati, Stefanie Jessica Henny Leenawaty Limantara Mada Triandala Sibero Mada Triandala Sibero Meinhard Simon Miftahuddin Majid Khoeri Misbakul Munir Muchlissin, Sakti Imam Muhamad Fikri Hudi Nur Hakim Muhamad Ziaul Faiz Muhammad Eka Darmawan Rafsanjani Muhammad Zainuri Nadya Cakasana Norma Afiati Norma Afiati Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Ocky Karna Radjasa Ocky Karna Radjasa ocky radjasa Popi IL Ayer Prastyo Abi Widyananto Puspa Kapinangasih Putut Har Riyadi Raden Ario Rafsanjani, Muhammad Eka Darmawan Ragil Saptaningtyas Raja Aditya Sahala Siagian Ratna Diyah Palupi Retno Hartati Rignolda Djamaludin Rina Setyowati Sulistiyoningrum Rini Pramesti Rivan Novianto Madilana Romadhon Romadhon Rory Anthony Hutagalung Rosa Amalia Rudhi Pribadi Rudiger Stöhr S Sukarmi S. Sulistiyani Sakti Imam Muchlissin Sakti Imam Muchlissin Sakti Imam Muchlissin Setiasih, Intan Budi Setyani, Wilis Ari Sibero, Mada Triandala Slamet Budi Prayitno Sri Achadi Nugraheni Sri Lintang Artono Sri Redjeki Stalis Norma Ethica Stefanie Jessica Henny Larasati Subagiyo Subagiyo Subagiyo Subagiyo Subagyo Subagyo Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sunaryo Sunaryo Suzana Kristy Satriani Fofied Syauqina Nashihi Aufar Thorsten Brinkoff TONNY BACHTIAR Tonny Bachtiar Tony Bachtiar Tony Bachtiar Torben Marten TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Ulfah Amalia Wahyuningsih, Candra Widy Febriansyah Wilis Ari Setyani Yeni Sulistiyani Yesaya Putra Pamungkas

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