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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Veteriner Journal of Aquatropica Asia JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Akuatika Indonesia Journal of Aquaculture Science Journal of Aquaculture and Fish Health Grouper: Jurnal Ilmiah Perikanan Jurnal Abdi Insani Journal of Marine and Coastal Science Bioscientist : Jurnal Ilmiah Biologi Rambideun : Jurnal Pengabdian Kepada Masyarakat Journal of Fisheries & Marine International Journal of Natural Science Studies and Development (IJOSS)
Woro Hastuti Setyantini
Department Of Aquaculture, Faculty Of Fisheries And Marine, Universitas Airlangga, Campus C, Mulyorejo Street, 60115 Surabaya, INDONESIA.
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Public Health Social Sciences Veterinary

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PENGARUH WAKTU PANEN DAN NUTRISI MEDIA TERHADAP BIOPIGMEN Spirulina platensis setyaningsih, Iriani -; Tarman, Kustiariyah -; satyantini, Woro hastuti; barus, dita aguistina
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 16 No. 3 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.596 KB) | DOI: 10.17844/jphpi.v16i3.8054

Abstract

Spirulina platensis merupakan alga hijau biru yang mengandung biopigmen fi kosianin dan antioksidanyang bermanfaat bagi kesehatan. Produksi biopigmen dipengaruhi oleh nutrien dan umur kultur. Penelitianini bertujuan menentukan pengaruh media dan umur panen terhadap kandungan fi kosianin dan aktivitasantioksidan. Kultivasi S. platensis dilakukan di dalam laboratorium menggunakan media KT, MT, dan Walne.Ekstraksi fi kosianin dilakukan menggunakan pelarut akuades dan bufer fosfat. Kandungan fi kosianin S.platensis dalam media MT (10,07 mg/mL) tidak berbeda nyata dengan media Walne (7,49 mg/mL), tetapiberbeda nyata dengan media KT (0,71 mg/mL). Aktivitas antioksidan S. platensis pada ketiga media tidakberbeda nyata. Kandungan fi kosianin S. platensis pada hari ke-12 (10,42 mg/mL) berbeda nyata dengan harike-6 (2,70 mg/mL), hari ke-14 (8,14 mg/mL), dan hari ke-17 (3,09 mg/mL). Aktivitas antioksidan S. platensispada keempat umur panen tidak berbeda nyata.Kata kunci: antioksidan, fi kosianin, Spirulina platensis, waktu panen
PENGARUH WAKTU PANEN DAN NUTRISI MEDIA TERHADAP BIOPIGMEN Spirulina platensis setyaningsih, Iriani -; Tarman, Kustiariyah -; satyantini, Woro hastuti; barus, dita aguistina
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 16 No. 3 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.596 KB) | DOI: 10.17844/jphpi.v16i3.8055

Abstract

Spirulina platensis merupakan alga hijau biru yang mengandung biopigmen fi kosianin dan antioksidanyang bermanfaat bagi kesehatan. Produksi biopigmen dipengaruhi oleh nutrien dan umur kultur. Penelitianini bertujuan menentukan pengaruh media dan umur panen terhadap kandungan fi kosianin dan aktivitasantioksidan. Kultivasi S. platensis dilakukan di dalam laboratorium menggunakan media KT, MT, dan Walne.Ekstraksi fi kosianin dilakukan menggunakan pelarut akuades dan bufer fosfat. Kandungan fi kosianin S.platensis dalam media MT (10,07 mg/mL) tidak berbeda nyata dengan media Walne (7,49 mg/mL), tetapiberbeda nyata dengan media KT (0,71 mg/mL). Aktivitas antioksidan S. platensis pada ketiga media tidakberbeda nyata. Kandungan fi kosianin S. platensis pada hari ke-12 (10,42 mg/mL) berbeda nyata dengan harike-6 (2,70 mg/mL), hari ke-14 (8,14 mg/mL), dan hari ke-17 (3,09 mg/mL). Aktivitas antioksidan S. platensispada keempat umur panen tidak berbeda nyata.Kata kunci: antioksidan, fi kosianin, Spirulina platensis, waktu panen
Growth of Nannochloropsis oculata Cultured using Tofu Liquid Waste Fertilizer Rahmadan, Rizky Yanuar; Satyantini, Woro Hastuti; Sari, Luthfiana Aprilianita
International Journal of Natural Science Studies and Development (IJOSS) Vol. 2 No. 1 (2025): IJOSS
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijoss.v2i1.18

Abstract

Growth of Nannochloropsis oculata is affected by nutrients in its culture media. Tofu liquid waste which is processed into fertilizer is expected to be an alternative nutrient that is more economical for microalgae culture activities. The purpose of this study was to determine the effect of tofu liquid waste fertilizer with different concentrations on the growth of Nannochloropsis oculata. This study used an experimental method with Completely Randomized Design (CRD). The treatment was different concentrations namely P1 (1 ml L-1), P2 (3 ml L-1), P3 (5 ml L-1), P4 (7 ml L-1) and P0 (Walne nutrient 1 ml L-1) each treatment were repeated four times. The main parameters in this study were cell density, cell growth rate and cell doubling time. Data obtained were analyzed using Analysis of Variance (ANOVA) and continued with Duncan's Multiple Range Test (DMRT). Results showed that the treatment of tofu liquid waste fertilizer with different concentrations had a significant different (p<0,05) on the growth of Nannochloropsis oculata. Concentration of 5 ml L-1 (P3) was the best concentration for the growth of Nannochloropsis oculata with peak cell density 1,335±13.54x104 cells ml-1, cell growth rate 0.900±0.001 cells ml-1 day-1 and cell doubling time 18.493±0.026 hours.
Polyvalent formalin-killed Aeromonas hydrophila vaccine with oil adjuvant drives coordinated humoral, innate, and cytokine responses in giant gourami (Osphronemus goramy) Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan 2026: JIPK VOLUME 18 ISSUE 1 YEAR 2026 (FEBRUARY 2026, ISSUE IN PROGRESS)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Bacterial septicaemia caused by Aeromonas hydrophila is an important constraint for giant gourami (Osphronemus goramy) culture in Southeast Asia. Inactivated whole-cell (bacterin) vaccines are widely used against bacterial diseases in aquaculture, but comparative data on monovalent versus polyvalent A. hydrophila vaccines, with and without oil-based adjuvant, remain scarce for this species. This study evaluated the safety, immunological responses, and protective efficacy of three formalin-killed cell (FKC) vaccines prepared from gourami-derived A. hydrophila isolates: a monovalent FKC (P2), a non-adjuvanted polyvalent FKC (P3), and an oil-adjuvanted polyvalent FKC (P4), using PBS (P1) as a control. Sub-adult giant gourami were vaccinated intraperitoneally and monitored for 42 days. Serum agglutinating antibody titres, nitroblue tetrazolium (NBT)-reducing activity, and splenic il-1β and ifn-γ mRNA expression were measured at multiple time points. At 21 days post-vaccination, a separate cohort was challenged intraperitoneally with virulent A. hydrophila Ah-S1, and survival was recorded for 14 days; relative percent survival (RPS) was calculated at day 14. All FKC formulations were clinically well tolerated, with only transient post-vaccination inappetence and no gross injection-site pathology. Vaccination induced clear, treatment- and time-dependent increases in agglutinating antibody titres, NBT-reducing activity, and splenic il-1β and ifn-γ transcription, with the strongest and most sustained responses in P4, intermediate responses in P3 and P2, and minimal changes in P1. Following homologous challenge, day-14 survival was 8.3% in P1, 61.7% in P2, 75.0% in P3, and 83.3% in P4, with corresponding RPS values of 58.2%, 72.7%, and 81.8%, respectively. Under these experimental conditions, the oil-adjuvanted polyvalent FKC (P4) produced the greatest enhancement of immune responses and protection against intraperitoneal A. hydrophila challenge in giant gourami. These findings support this formulation as a candidate for further vaccine development in O. goramy and highlight the need for dose optimisation, safety assessment, heterologous challenge, and field-validation studies before recommendations for large-scale use in aquaculture.
Co-Authors A. Shofy Mubarak Abdul Manan Abdul Manan Adriana Monica Sahidu Agustono - Agustono . Agustono Agustono Agustono Agustono Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Alim Isnansetyo Alvira Febrianti Pratiwi Anam, M Khairul Ananta Kurniawan Andry Wijayanto Anggun Nurani Citrowati Annas Salleh Ariyandra, Rheza Asmaul Khusnah Aulia Ikhsan Syamsuri Ayu Puspitarani Boedi Setya Rahardja Brian Zuliyan Browijoyo Santanamurti Catur Pujiono Cicik Kurniawati, Cicik Daruti Dinda Nindarwi Daruti Dinda Nindarwi Dewi, Nina Nurmalia Dimas Eraparamarta Harkitiyanto dita aguistina barus Dita Wisudyawati Djamil, Hasim Dwitha Nirmala Elangga Sony Widiharsono Emy Koestanti Sabdoningrum Enang Harris Faisol Mas’ud Falatehan, Naufal Febri Setyawati Firdaus, Fitrul Azizi Fuquh Rahmat Shaleh Gazali Salim Gunanti Mahasri Ika Purnamasari Isroni, Wahyu Juanda Juanda Karina Rahardjo Kismiyati , Kuncorojakti, Suryo Kustiariyah Tarman Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Lia Oktavia Ika Putri Lilis Cahaya Septiana Lina Wafia Asmi Luthfiana Aprilianita Sari M Wahyu Alfian Manik, Melani Indah Sari Meilina, Sinta Cahya Mohammad Noor Amal Azmai Muchammad Yunus Muhamad Amin Muhammad Amin Muhammad Arief Muhammad Izzudin Syaifullah Muhammed Jefri Myrna Budi N. Juni Triastuti Naila Budiatin Wahyu Mufidah Nizar Afiansyah Loekman Nova Erika Nur Bambang Priyo Utomo Okky Hermawan Panhar, Fio Surya Pratama Semendo Prayogo Prayogo Pursetyo, Kustiawan tri Putri Desi Wulan Sari Rachmat Rizaldi Rahayu Kusdarwati Rahmadan, Rizky Yanuar Rahmahani, Jola Rahmawati, Ghishella Ayu Rheza Ariyandra Rozi Rozi Rozi Rozi Rozi Saiful Bakhri Salman Aldo Alfaresi Saniya Lailatul Qodriyah Santanumurti, Muhammad Browijoyo Sapto Andriyono Saragih, Friska Setiawani Sari, Luthfiana Aprilanita Septuresty Hartri Eka Sihombing, Agusmanto Sudarno, Sudarno Sukenda . Suwarno Suwarno Vega Chrisnawati Veryl Hasan Vivaldy Phaza Muharta Wiwiek Tyasningsih Yudi Cahyoko