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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Jurnal Nutrisi Ternak Tropis (JNT) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Bantara Journal of Animal Science journal of Basic Medical Veterinary Jurnal Agro Veteriner
Agustono, Bodhi
Departemen Peternakan, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C UNAIR Mulyorejo, Surabaya, Jawa Timur, Indonesia 60115
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

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Prevalence and Risk Factors of Gastrointestinal Helminth Infection in Pet Cats in Wonogiri District, Central Java Sekarsari, Dara Ayu Pramesti; Saputro, Amung Logam; Agustono, Bodhi; Yunita, Maya Nurwartanti; Praja, Ratih Novita; Solikhah, Tridiganita Intan
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.49559

Abstract

Gastrointestinal helminth infection is the most common and the easiest infection to infect wild cats or pet cats but is often ignored by the cat owners because sometimes there are no symptoms or a lack of attention from the cat owner to their pet cat. The aim of this study was to estimate the prevalence and risk factors of gastrointestinal helminth infection in pet cats in Wonogiri District, Central Java. A total of 100 cat samples were evaluated using a questionnaire, then fecal microscopic examination using simple native method, sedimentation, and floatation methods. Data obtained from this study were presented descriptively and analyzed using the Chi-Square test. The result showed that 37 out of 100 fecal samples were found positive infected with gastrointestinal helminth including Toxocara cati and Dipylidium caninum or the prevalence of gastrointestinal helminth infection can be calculated at 37%. The result of the analysis of significant risk factors were breed, number of pet cat owners, grooming, deworming, and vaccination.
The addition of vitamin C in tris–egg yolk extender maintained Sapera goat semen quality in 5° C storage Pahlevy, Jolly Rifqi; Ratnani, Hermin; Restiadi, Tjuk Imam; Fikri, Faisal; Saputro, Amung Logam; Agustono, Bodhi
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.1-8

Abstract

Goats are small ruminants that are reared by the rural community for financial income and nutrition. This study was aimed to determine the effect of vitamin C addition in tris–egg yolk extender on the lifespan of Sapera goat spermatozoa stored in 5o C. Semen was collected from 1.5 years old Sapera male goat. The ejaculates were diluted in Tris-egg yolk (T-EY) extender added with 0, 0.2, 0.3, and 0.4 g vitamin C/mL extender for T0, T1, T2, and T3 groups, respectively. The spermatozoa motility, viability, and morphological abnormality were assessed along with storage at 5° C. The result showed that spermatozoa motility was the highest (p <0.05) in T1 compared to other groups and qualified for AI use for up to 72 hours. In 24 hours storage, the spermatozoa viability was not significantly different (p >0.05) among the groups. The spermatozoa viability in the T1 group was the highest (p <0.05) among the groups along 48-96 hours of storage. The spermatozoa morphological abnormalities of the T1 group was the lowest (p <0.05) compared to other treatment groups in the range of 24-72 hour storage. In the control group (T0), the less than 5% spermatozoa morphological abnormalities (qualified for artificial insemination) were only in the 24 hours storage, while those of the T1 group were up to 72 hours. It could be concluded that the addition of 0.2 g vitamin C/100 mL T-EY extender maintained the quality of Sapera goat semen for 72 hours at 5°C.
Pre-freezing at 10 cm above liquid nitrogen surface for eight minutes resulted the best Sapera goat semen quality Pasha, Zehansyah Algifari; Agustono, Bodhi; Saputro, Amung Logam
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.27-33

Abstract

Sapera goat is a dairy goat resulting from a cross between a Saanen buck and an Ettawah cross doe. These small ruminants are reared by rural households for poverty reduction and undernutrition prevention. Breeding of Sapera goat through artificial insemination (AI) technique was expected to accelerate the increase in population. Unfortunately, the frozen semen of Sapera buck is not available yet. This study aims to determine the effect of the distance between semen straw and liquid nitrogen surface during pre-freezing process on the quality of frozen semen of Sapera goats. Semen was obtained from 1.5 years old Sapera buck. The ejaculates were diluted in tris-egg yolk extender to obtain a concentration of 120 x 106 spermatozoa/mL, then equilibrated at 5°C for one hour. The extended semen was packaged in 0.5 mL French straws (60 x 106 spermatozoa/straw). The filled straws were pre-frozen in the cold handling cabinet (Minitube, Germany) held at 10, 11, 12, 13, 14, and 15 cm above liquid nitrogen respectively for T1, T2, T3, T4, T5, and T6 groups, each with four replicates. After pre-freezing for eight minutes the straws were immediately plunged, and stored in liquid nitrogen (-196°C) for two days, followed by quality assessment. The best post-thawed motility (41.75 ± 2.06 %), viability (49.00 ± 0.82 %) and morphological abnormalities (4.75 ± 0.96 %) were obtained from pre-freezing stage with a distance of 10 cm. It could be concluded that in tris-egg yolk extender, post-thawed spermatozoa viability and motility of Sapera buck were qualified for AI when fresh semen was pre-frozen at 10 cm distance of straws from the surface of liquid nitrogen for eight minutes. 
Probiotics as Alternatives to Antibiotic Growth Promoters in ISA Brown Males Chicken Afililla, Zhaza; Agustono, Bodhi; Marbun, Tabita Damaria; Vedy, Dayang Urmila Shahnaz; Yulisah, Dina; Riastianto, Muhammad Rofiq; Firmansyah, Muhammad Alif Rifqi; Farihah, Diniah Umy; Zahra, Reyza Miladiya Khuriyah; Afandik, Nadya Ashila; Ghultom, Astri Agustina; Meiliza, Nazwa; Nugroho, Adi; Prima, Jaya Agusc
Media Kedokteran Hewan Vol. 37 No. 1 (2026): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v37i1.2026.22-30

Abstract

Antibiotic Growth Promoters (AGP/AGPs) have been used to improve feed efficiency in poultry, but are banned in Indonesia due to food safety concerns. This study evaluated the potential of probiotics as an AGP alternative in ISA Brown male layer chickens during the finishing period, focusing on blood glucose and cholesterol levels. A total of 180 chickens were reared for 42 days. Treatment groups received basal feed supplemented with probiotics at 1–5 mL/kg, equivalent to 1.2×10⁹ to 6×10⁹ CFU/kg feed, containing Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium spp. Blood cholesterol was analysed using an enzymatic colorimetric method at 500 nm, while glucose was measured using the glucose oxidase method at 505 nm. Results showed that probiotic treatments (P2, P3, P5) significantly reduced glucose levels compared to the control (P0) and AGP group (P1). A moderate decrease in cholesterol levels was also observed in probiotic groups. These effects may be linked to improved gut microbial balance and nutrient metabolism. The probiotic combination used in this study shows potential as a safer and more sustainable alternative to AGPs. Further studies are recommended to optimize dosage and assess long-term impacts on poultry health and productivity.
PROTECTIVE EFFECTS OF COMBINED VITAMIN E AND ZINC SULFATE ON OVARIAN AND UTERUS WEIGHT OF ALBINO RATS (Rattus norvegicus) EXPOSED TO LEAD ACETATE Fitriani Sultan; Tri Wahyu Suprayogi; Rimayanti; Ragil Angga Prastiya; Bodhi Agustono; Amung Logam Saputro
VITEK : Bidang Kedokteran Hewan Vol. 16 No. 1 (2026): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/594dbr52

Abstract

Lead exposure exerts toxic effects on the female reproductive system, particularly on the ovaries and uterus, resulting in hypofunction and organ atrophy. Oxidative stress induced by lead accumulation may be attenuated by antioxidants such as vitamin E and zinc sulfate. This study aimed to evaluate the protective effects of vitamin E, zinc sulfate, and their combination on ovarian and uterus weights in albino rats (Rattus norvegicus) exposed to lead acetate. Twenty-five female rats were randomly divided into five groups (K, T0, T1, T2, and T3) and treated orally for 21 days. On day 22, the rats were euthanized, and the ovaries and uterus were collected and weighed. The average ovarian weights for K, T0, T1, T2, and T3 were 25.80 ± 2.49; 18.62 ± 4.36; 28.40 ± 5.67; 33.78 ± 6.09; and 36.84 ± 5.15 mg, respectively. The average uterus weights for K, T0, T1, T2, and T3 were 292.48 ± 36.81; 249.96 ± 25.97; 315.86 ± 26.56; 325.54 ± 31.53; and 375.00 ± 36.55 mg, respectively. In conclusion, vitamin E and zinc sulfate demonstrated protective effects against lead-induced reproductive toxicity, with the combined treatment showing the greatest improvement in ovarian and uterus weights.
Towards a Micro-Circular Economy in Poultry Production: Integrating Probiotic Substitution and Waste Valorization to Mitigate Environmental Impact Bodhi Agustono; Maya Nurwartanti Yunita; Zhaza Afililla; Ragil Angga Prastiya; Suciyono; Faizal Ulhaq; Mirza Atikah Madarina Hisyam; Widya Paramita Lokapirnasari
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.83263

Abstract

The intensification of the global poultry sector is currently challenged by the need to balance productivity with the mitigation of antimicrobial resistance (AMR) and environmental pollution. This study addresses the technological gap among smallholder farmers in Banyuwangi, Indonesia, by implementing a comprehensive technology transfer program focused on the functional substitution of antibiotic growth promoters (AGPs) with probiotics and the development of waste management cycles. Employing a Participatory Action Research (PAR) framework, the intervention integrated cognitive coaching, technical demonstrations, and longitudinal field applications to bridge the divide between biotechnological innovation and grassroots practice. Data were obtained from 27 broiler farmers through pre- and post-test evaluations and Focus Group Discussions (FGDs) to measure literacy shifts and technical adoption. Quantitative analysis revealed a significant transformation in biotechnology literacy, with understanding of probiotic mechanisms increasing from 37.0% (baseline) to 85.2% post-intervention. Furthermore, technical competence in functional feed formulation improved by 33.4% (p < 0.05), while the intention to adopt sustainable practices increased to 96.3%. These findings indicate that barriers to adoption among smallholders are primarily driven by limited access to applied science rather than cultural resistance. The study concludes that this hybrid extension model successfully establishes a viable micro-circular economy, offering a replicable strategy for upstream AMR mitigation in developing agropolitan regions.
Potential of Bajakah Tampala Root Extract (Spatholobus littoralis Hassk) as an Antibacterial Agent Against Vibrio harveyi Kana Maulida; Iwan Sahrial Hamid; Maslichah Mafruchati; Bodhi Agustono; Amung Logam Saputro
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.87807

Abstract

Antibiotics are commonly used to treat vibriosis in shrimp. However, their prolonged use at high doses may lead to the development of antibiotic resistance. As a preventive measure against antibiotic resistance, the use of natural antibacterial agents is required. Bajakah tampala root (Spatholobus littoralis Hassk) has been reported to possess antibacterial properties. This study employed six treatment groups: a positive control using a tetracycline antibiotic disc, a negative control using distilled water and CMC-Na, and four treatment groups (P1, P2, P3, and P4) treated with Bajakah tampala root extract (Spatholobus littoralis Hassk) at concentrations of 25%, 50%, 75%, and 100%, respectively. After treatment, the diameters of the inhibition zones were measured. The data obtained were analyzed using the Shapiro–Wilk normality test, followed by one-way analysis of variance (ANOVA). The results showed that treatment P4, with a 100% concentration of Bajakah tampala root extract (Spatholobus littoralis Hassk), produced an inhibition zone diameter comparable to that of the positive control.
Co-Authors Adi Nugroho Afandik, Nadya Ashila Al arif, Mohammad Anam Boedi Setiawan Burhanudin Erma Safitri Faisal Fikri Faizal Ulhaq Farihah, Diniah Umy Firmansyah, Muhammad Alif Rifqi Fitriani Sultan Ghultom, Astri Agustina Hanifah, Novi Fitria Hermin Ratnani Iskandari, Novia Nur Iwan Sahrial Hamid Kana Maulida Khafsah, Khafsah Lailiyah, Fahlevi Ma'ruf, Anwar Mafruchati, Maslichah Marbun, Tabita Damaria Meiliza, Nazwa Mirni Lamid Mirza Atikah Madarina Hisyam Muhammad Thohawi Elziyad Purnama Mumtazi, Fauzan Nusdianto Triakoso Pahlevy, Jolly Rifqi Pasha, Zehansyah Algifari Poedji Hastutiek Praja, Ratih Novita Prastika, Zeni Prastiya, Ragil Angga Prayogo, Danu Prima, Jaya Agusc Primarizky, Hardany Pudji Srianto Rachmanita, Rona Taufiqul Rahmahani, Jola Ratna Damayanti Riastianto, Muhammad Rofiq Rimayanti Salsabila Putri, Elma Saputro, Amung Logam Saraswati, Adyayutti Wijang Sekarsari, Dara Ayu Pramesti Setyawan, Firman Siti Komariyah Solikhah, Tridiganita Intan Sri Pantja Madyawati Suciyono, Suciyono Suherni Susilowati Sulaeman, Nur Sa'adah Sunarso, Agus Tito, Muhammad Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Vedy, Dayang Urmila Shahnaz Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Yudhana, Aditya Yulianto, Rangga Yulisah, Dina Yunita, Maya Nurwartanti Zahra, Reyza Miladiya Khuriyah Zhaza Afililla