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Fetmi Silvina
Program Studi Agroteknologi, Fakultas Pertanian Universitas Riau Kampus Bina Widya Km 12,5 Simpang Baru Pekanbaru 28293
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Journal : Jurnal Agro

 1 
Induksi kalus daun binahong merah (Basella rubra L.) dengan pe,berian 2,4-D dan kinetin Fetmi Silvina; Isnaini Isnaini; Widya Ningsih
Jurnal Agro Vol 8, No 2 (2021)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/14273

Abstract

Tanaman binahong merah (Basella rubra L.) merupakan salah satu tanaman yang mengandung senyawa metabolit sekunder berkhasiat obat. Kultur kalus adalah salah satu solusi dalam menghasilkan senyawa metabolit sekunder dengan jumlah yang besar.  Penelitian ini bertujuan untuk mengetahui pengaruh pemberian 2,4-D dan kinetin dalam menginduksi kalus daun binahong merah. Penelitian dilakukan di Laboratorium Bioteknologi Tanaman Fakultas Pertanian Universitas Riau pada bulan November 2019 sampai Maret 2020. Percobaan menggunakan rancangan acak kelompok yang terdiri dari dua faktor yaitu 2,4-D dengan empat taraf konsentrasi yaitu 0 ppm, 0,5 ppm, 1 ppm dan 2 ppm dan kinetin dengan empat taraf konsentrasi yaitu 0 ppm, 0,5 ppm, 1 ppm dan 2 ppm dengan tiga ulangan. Hasil penelitian menunjukkan bahwa kombinasi perlakuan tanpa 2,4-D dan 0,5 ppm kinetin memberikan respon terbaik pada saat muncul kalus 11,67 HST dan perlakuan 1 ppm 2,4-D dan 2 ppm kinetin memberikan respon terbaik pada persentase keberhasilan induksi kalus 62,50 %.  Red binahong (Basella rubra L.) is a plant that contains medicinal secondary metabolites.  Callus culture is one solution in producing secondary metabolites in large quantities. This research aimed to determine the effect of 2,4-D and kinetin in inducing callus on red binahong leaves. The research was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, the University of Riau from November 2019 to March 2020. The experiment used a randomized block design with two factors, namely four levels of 2,4-D 0, 0.5, 1, and 2 ppm and four levels of kinetin namely 0, 0.5, 1, and 2 ppm with three replications. The results showed that a combination of 0 ppm 2,4-D and 0.5 ppm kinetin and 1 ppm 2,4-D and 2 ppm had the fastest callus formation 11.67 DAP and a combination of 1 ppm 2,4-D and 2 ppm kinetin produced weigher callus 6.4 mg and had a percentage of callus formation 62.50%.
Co-Authors ', Ardian ', Evawani ', Murniati ', Syafrinal Aceng Sumpena Adi Sucandra Agung Prassetio Agus Sutikno Ali Djamhuri Amrul Khoiri Anak Agung Istri Sri Wiadnyani Andri Ansyah Ansyar, Ihsan Azzam Anthony Hamzah AR, Arman Effendi Ardian Ardian Ardian Ardian Arif Fashli Hakiki Arjuna Kesuma Armaini ' Armaini ' ' Armaini, Armaini Arman Effendi Arnis en Yulia Arnis En Yulia Arnis En Yulia Arnis En Yulia Arnis En Yuliana Aslim Rasyad Besri Nasrul Boby Azlansyah Charles Charles Cica Yulia Mandela1 Dedi Mulyadi Desita Salbiah EKO WAHYUDI Elza Zuhry Erlida Ariani Esyka ' ' Evawani ' Fachry Abda El Rahman Fauziah Mayasari Fifi Puspita Foller, Rudi Gea, Berkat Harapan Jaya Gianka Pratama Harsan Gunawan ' Tabrani Gusmawartati Gusmawartati Hafiz Fauzana Hakiki, Arif Fashli Hazmi, Wahyu Hervina Ramalia Hutasoit, Preddi Gunawan Monontong Idwar, Idwar Ielham Ekha Satria, Ielham Ekha Indradewa, Rhian Irene Farida Widawaty Irfandri Irfandri Irfandri, Irfandri Isna Rahma Dini Isnaini Isnaini Isnaini Isnaini Isnaini Isnaini Isnaini, Isnaini Joni Adri Joni Irawan, Joni Juniarika Silalahi Kartika, Titik Dwi Laksono Trisnantoro Lendri Irawan Lubis, Nursiani M Amrul Khoiri M. Amrul Khoiri Marlisa ' Mestika Hutagalung Mhd. Rizki Hidayu Mita, Nastra Miya Ramadhani Mora, Nur Fadila MUHAMMAD ALI Muhammad Ma'ruf Muntazar, Mhd Roqi Murniati ' Murniati . Murniati Murniati Murniati Murniati Murniati Nawaruddin Nawaruddin Nazara, Marniati Nelvia Nelvia Nelvila, Olva Noreza Masri Norman Sahputra, Norman Nur Fadila Mora Nuratika, Suci Nurbaiti ' Nurhayati Nurhayati Nurhidayah, T. Pajri Ananta Yudha Pebrianti, Riska Perdi Perdi Rahmi Saputri, Rahmi Rahmita, Nurfa Ramadani, Ramadani Ramadhoni, Rizky Ramayani, Dwi Ricky Saputra Sinaga Riska Pebrianti Rizki, Nisa Alifia Rizky Ramadhoni Roy Ibrahim Rusli Rustam Rusli Rustam Sinambela, Flora Krisdyanti Sisgianto Sisgianto Sri Yoseva Suprianto ' Susilawati Susilawati Susilawati, Susilawati Syafrinal ' Syafrinal, Syafrinal Syahputra, Anugrah Kharisma Tengku Nurhidayah Trizayuni, Riska Wardati Wardati Wardati, Wardati Wawan ' Wawan Wawan Widya Ningsih Wulandari, Chicha Yazid Suhadi Yuda, Pajri Ananta Yudi Firmansyah Yulinda Ari Indah Karunia Yunita Fitri Zafitra, Zafitra Zaidan Ersyad Zulham Zulham