Article Per Year (5 Year)
p-Index From 2021 - 2026
1.383
P-Index
This Author published in this journals
All Journal Jurnal Natur Indonesia Media Penelitian dan Pengembangan Kesehatan Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Alchemy Jurnal Penelitian Kimia PROSIDING SEMINAR KIMIA Biopropal Industri Medika Tadulako INDONESIAN JOURNAL OF PHARMACY BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Fitofarmaka Indonesia Chimica et Natura Acta Farmasains : Jurnal Ilmiah Ilmu Kefarmasian ANNALES BOGORIENSES JURNAL ATOMIK Biosaintifika: Journal of Biology & Biology Education JSFK (Jurnal Sains Farmasi & Klinis) FITOFARMAKA: Jurnal Ilmiah Farmasi Pharmaceutical And Biomedical Sciences Journal (PBSJ) Journal of Social Research Jurnal Kefarmasian Indonesia Kimia Padjadjaran Annales Bogorienses
Partomuan Simanjuntak
Pusat Penelitian Bioteknologi Lembaga Ilmu Pengetahuan Indonesia Cibinong, Jawa Barat
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

Published : 35 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 35 Documents
Search

 1   2   3   4 
AKTIVITAS ANTIOKSIDAN EKSTRAK KAPANG ENDOFIT Cb.Gm.B3 ASAL RANTING KAYU MANIS (Cinnamomum burmanni) Rachman, Fauzy; Mubarik, Nisa Rachmania; Simanjuntak, Partomuan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9174.357 KB) | DOI: 10.29122/jbbi.v5i2.3052

Abstract

Antioxidant Activity of Endophytic Fungi Cb.Gm.B3 Extract from Cinnamon (Cinnamomum burmanni) TwigsABSTRACTThere are many degenerative diseases that are caused by a free radical effect. Cinnamon (Cinnamomum burmanni) contains cinnamaldehyde compounds that have activity as a powerful antioxidant and fight free radicals. Endophytic fungi can be found in cinnamon plants living symbiotically. Endophytic fungi produce a variety of bioactive metabolites including antioxidants. This research was conducted to isolate endophytic fungi from C. burmanni plant which is active as antioxidant. Endophytic fungi isolation was carried out using surface sterilization method and cultivated in PDA media. Antioxidant activity test was performed using free radical 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. Selected isolates were then identified molecularly to determine their species. A total of nine fungi were isolated from cinnamon twigs. The result showed that the highest antioxidant activity was obtained from Cb.Gm.B3 with IC50 of 13.219 ± 0.755 µg/mL. The selected isolate Cb.Gm.B3 taxonomically has a high similarity with Neofusicoccum parvum isolate PEL23 (Accession no: KY053054.1).Keywords: antioxidant, Cinnamomum burmanni, 2.2-diphenyl-1-picrylhydrazyl, endophytic fungi, Neofusicoccum parvum ABSTRAKKayu manis (Cinnamomum burmanni) mengandung senyawa sinamaldehid yang memiliki aktivitas sebagai antioksidan kuat dan dapat menangkal radikal bebas. Dalam tanaman kayu manis terdapat kapang endofit yang hidup bersimbiosis. Kapang endofit dapat menghasilkan berbagai senyawa metabolit bioaktif termasuk antioksidan. Penelitian ini dilakukan untuk mengisolasi kapang endofit dari tanaman C. burmanni yang aktif sebagai antioksidan. Isolasi kapang endofit dilakukan menggunakan metode sterilisasi permukaan dan ditanam pada media PDA. Pengujian aktivitas antioksidan dilakukan menggunakan metode peredaman radikal bebas dengan reagen 2.2-difenil-1-pikrilhidrazil (DPPH). Isolat terpilih diidentifikasi secara molekuler untuk menentukan spesiesnya. Sebanyak 9 isolat kapang berhasil diisolasi dari jaringan ranting tanaman kayu manis. Aktivitas antioksidan tertinggi (IC50) didapatkan dari isolat Cb.Gm.B3 sebesar 13,219 ± 0,755 µg/mL. Isolat terpilih Cb.Gm.B3 secara taksonomi memiliki tingkat kemiripan yang tinggi dengan Neofusicoccum parvum isolat PEL23 (No. aksesi: KY053054.1).Kata Kunci: antioksidan, Cinnamomum burmanni, 2.2-difenil-1-pikrilhidrazil, kapang endofit, Neofusicoccum parvum
Antioxidant Activity of Endophytic Fungi Isolated from Turmeric Flowers Septiana, Eris; Yadi, Yadi; Simanjuntak, Partomuan
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24396

Abstract

Antioxidants are compounds needed by the body to fight free radicals that can cause adverse effects on health. All parts of turmeric plant have antioxidant activity, so does the flower. It is thought that the endophytic fungi from turmeric flowers also have antioxidant activity and no studies have reported it yet. The purpose of this study was to study the antioxidant activity of single and mixed cultures of endophytic fungi isolated from turmeric flowers. In vitro antioxidant activity assay was conducted using the DDPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method by measuring its absorption using a UV-Vis spectrophotometer at a wavelength of 517 nm. The extract of single and mixed cultures of endophytic fungi isolated from turmeric flowers showed antioxidant activity. The combination of fungal cultures can increase the antioxidant activity of a single culture. This study is the first to report that the combination of endophytic fungi from turmeric flowers can increase its antioxidant activity. Therefore, the presence of the endophytic fungi in turmeric flowers that have antioxidant activity can provide additional information so that the utilization of turmeric flowers as a source of natural antioxidants will increase.
Aktivitas Fraksi Air Kulit Batang Mahoni (Swietenia macrophylla King.) dan Studi In Silico Senyawa Kimia Penghambat Enzim α-Glukosidase Khaerunnisa, Arini; Djamil, Ratna; Sulastri, Lilik; Simanjuntak, Partomuan
Jurnal Fitofarmaka Indonesia Vol 9, No 1 (2022): JURNAL FITOFARMAKA INDONESIA
Publisher : Jurnal Fitofarmaka Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v9i1.800

Abstract

 Inhibition of the α-glucosidase can be used as antidiabetic therapy because it can reduce postprandial blood sugar levels. The stem bark of mahogany (Swietenia macrophylla King.) have properties as inhibitors of enzyme α-glucosidase. The purpose of this study was to determine the active compound of mahogany stem bark against α-glucosidase enzyme in vitro and in silico. 96% ethanol extract of mahogany stem bark was partitioned with n-hexane, ethyl acetate and water. Testing of inhibition of α-glucosidase enzyme work done with substrate p-nitrophenyl-α-D-glucopyranoside. The water fraction was separated on silica gel column chromatography (SiO2 : dichloromethane-methanol = 10 : 1 ~ 1 : 1 and methanol). Molecular docking using AutoDock 4.2.6 was performed to predict the binding modes of α-glucosidase enzyme with chemical compound from water subfraction of stem bark mahogany. The results showed that water fraction of stem bark mahogany have antidiabetic activity with IC50 value of 1.51 µg/mL. Fraction Fr-1 gave an inhibition of 83.61 ± 1.11 %. Fr-1 contains chemical compound catechin, evodionol, swietenitin E, 11-deoxyswienialide D, swietenitin J and H. In silico study showed that catechin and evodionol free energy value (ΔG) -6.98 and -6.25 Kcal/mol and inhibition constanta (Ki) 7.61 and 26.18 µM.
Potensi Ekstrak Kapang Endofit Asal Rimpang Kunyit sebagai Antimalaria dan Antioksidan Septiana, Eris; bustanussalam, Bustanussalam; Rachman, Fauzy; Simanjuntak, Partomuan
Jurnal Kefarmasian Indonesia VOLUME 7, NOMOR 1, FEBRUARI 2017
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v7i1.3482

Abstract

Malaria infection is still a public health problem, especially in developing countries, including Indonesia. Malaria infection is usually accompanied by increased free radicals in the body of the patient. This situation will cause decreasing the immune system of the patient. Explorating of new drugs that have antimalarial and also antioxidant activity is very important. Therefore, this study aims to determine in vitro antimalarial and antioxidant activity of endophytic fungi extract origin turmeric from Sukabumi. This study begins with fermentation process of endophytic fungi on the broth medium and ethyl acetate was used to extract the filtrat to gain the test extracts. Heme polymerization inhibition and free radical DPPH scavenging method were used to antimalarial and antioxidant in vitro assay respectively. The results showed that 18 isolates of endophytic fungi have antimalarial activity and one isolate inactive and also all isolates have antioxidant activity. Smi.Cl.6F isolate was the most active isolate on the antimalarial and antioxidant assay with IC50 value of each assay were 1.93 mg/mL and 32.28 mg/L respectively. Therefore, Smi.Cl.6F isolates potentially be used as new antimalarial drugs
Efek Sinergis dari Kombinasi Ekstrak Herba Pegagan (Centella asiatica) dan Ekstrak Daun Kelor (Moringa oleifera) dalam Meningkatkan Fungsi Memori Yudhi Purwoko, Marcus Laurentius; Syamsudin, Syamsudin; Simanjuntak, Partomuan
JSFK (Jurnal Sains Farmasi & Klinis) Vol 9 No 2 (2022): J Sains Farm Klin 9(2), Agustus 2022
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.9.2.111-120.2022

Abstract

Otak merupakan pusat syaraf yang sangat berpengaruh terhadap respon anak untuk melihat, mendengar, berpikir, dan melakukan gerakan. Terganggunya fungsi kognitif mengakibatkan terhambatnya perkembangan pertumbuhan dan mempengaruhi kualitas masa depannya khususnya kecerdasannya. Tujuan penelitian ini adalah untuk mengetahui konsentrasi terbaik dari kombinasi ekstrak herba pegagan (Centella asiatica) dan ekstrak daun kelor (Moringa oleifera) dalam meningkatkan fungsi memori dan pembelajaran. Metode yang dilakukan adalah Radial 8-arm maze test yang digunakan untuk mengetahui adanya brain disorder yang berupa defisit pada pembelajaran dan memori serta menggunakan Passive avoidance test untuk mengetahui memori dan fungsi kognitif otak. Histopatologi otak diperiksa dengan menghitung jumlah sel neuron pada CA 1, CA 3 dan DG. Hasil pengujian menunjukkan bahwa kombinasi ekstrak herba pegagan dan daun kelor perbandingan 1:1 meningkatkan fungsi memori dan pembelajaran. Hasil histopatologi otak menunjukkan bahwa kombinasi ekstrak aman untuk digunakan
BIOPRODUKSI SENYAWA KIMIA ANTIDIABETES OLEH ISOLAT KAPANG ENDOFIT KUMIS KUCING (Orthosiphon spicatus B. B. S.) Sulastri, Lilik; Kartika, Rudi; Simanjuntak, Partomuan
PROSIDING SEMINAR KIMIA Vol 3 No 1 (2024): Prosiding SNK 2024
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kumis kucing plant (Orthosiphon spicatus B. B. S.) are one of the plants that have been used as diuretic, antidiabetic and antihypertensive drugs. Identification of chemical compounds, and antidiabetic activity assay of bioproduction of endophytic fungi with code B.Os.1F that isolated from the stems of kumis kucing have been carried out. Two fermentation methods (dynamic and static fermentation) to determine the pH. The tlc profile analysis and pH showed that B.Os.1F isolate by dynamic fermentation for 12 days had the potential to produce chemical compounds as antidiabetics at pH 4 ~ 6. The product of fermentation are extracted with ethyl acetate, then fractionated by column chromatography (SiO2): (i) n-hexane-ethyl acetate [10:1 ~ 1:1]; (ii) chloroform-methanol (20:1 ~ 1:1) based on “bioassay guided fractionation” with a-glucosidase enzyme inhibition assay. The isolate of B.Os.1F.2-3 had an activity of 76 %, and identify based on UV-Vis, Fourier-Transform Infra Red (FT-IR) spectroscopies and gas chromatography-mass spectrometer (GC-MS) are estimated to be Ergosta-5,7,9(11),22-tetraen-3-ol compounds. Keywords : Endophytic fungus, Orthosiphon spicatus B.B.S., α-glucosidase enzyme, ergosta-5,7,9(11),22-tetraen-3-ol.
Antioxidant Activity of Bioproduced Endophytic Fusarium Sp. HSFP-3 from Sijangkang Plant (Hornstedtia scyphifera Var. fusiformis) Koraag, Alfa Michael Gabriel; Farida, Yunahara; Tarigan, Elsera Br.; Efendy, Oscar; Agusta, Andria; Simanjuntak, Partomuan
Jurnal Fitofarmaka Indonesia Vol 11, No 3 (2024): JURNAL FITOFARMAKA INDONESIA (ENGLISH EDITION)
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v11i3.1252

Abstract

Endophytic mold Fusarium sp. HSFP-3 is one of the endophytic molds isolated from Sijangkang plants (Hornstedtia scyphyfera var. fusiformis). The purpose of this study is to conduct bioproduction, and test the antioxidant activity of bioproduced extracts and identify active fractions that have antioxidant activity. The research method started from the cultivation of endophytic mold HSFP-3 on PDB media for 21 days, the bioproduction results were extracted using ethyl acetate and acetone solvents. Antioxidant activity of HSFP-3 extract was determined by IC50 and AAI values, using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Isolation of antioxidant active fraction was carried out by column chromatography method using sephadex LH-20 stationary phase and silica. Isolation of antioxidant active compounds of HSFP-3 extract using bioautography guided assay approach with TLC-DPPH Bioautography method. The antioxidant test results of HSFP-3 extract has moderate activity with IC50 value 74,64 and AAI 0,844. Fraction 2.4 is an active fraction that has spot compounds with higher yellow color intensity compared to other fractions. Spot with Rf 0.35 in fraction 2.4 has a dark zone under UV 254 light. has a sea blue color under UV 366 light and a dark purple color after with Vanillin-Sulfate reagent
ISOLATION OF CHEMICAL ACTIVE COMPOUNDS ANTIOXIDANT FROM ETHYL ACETATE FRACTION OF BETEL LEAF FOREST (Piper aduncum L.) Batan, Alfrida; Daniel, Daniel; Simanjuntak, Partomuan
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.259 KB)

Abstract

Research about the isolation of chemical active compounds antioxidants from ethyl acetate fraction of betel leaf forest (Piper aduncum L.) have been done. This research aimed to determine the type of secondary metabolites that contained in ethyl acetate fraction and the value of antioxidant activity as well as the structure that contained in ethyl acetate fraction. The test results of phytochemical showed that the secondary metabolites in ethanol extract are alkaloids, steroids and triterpenoids. The inhibitory concentration 50% (IC50) value that shown in the fraction Piper aduncum ethyl acetate-4-3 (PAEA-4-3) is 132.84 ppm. Fraction of PAEA-4-3 that have high levels of antioxidant activity was analyzed using a spectrophotometer Ultra Violet-Visible (UV-Vis), Infrared Fourier-Transform (FTIR) and Nuclear Magnetic Resonance (NMR) proton and carbon. The result analysis of PAEA-4-3 isolates showed the compound as 2,3-dimethoxy-metilanaoksi-benzene.
Aktivitas Antimalaria Ekstrak Air dan Etanol Tanaman Obat Berdasarkan Penghambatan Pembentukan β-Hematin Hapsari, Yatri; Kusharyoto, Wien; Simanjuntak, Partomuan
Kimia Padjadjaran Vol 1, No 1 (2022)
Publisher : Kimia Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Malaria merupakan penyakit yang disebabkan oleh parasit Plasmodium sp. melalui gigitan nyamuk Anopheles sp. betina. Salah satu mekanisme parasit yang dijadikan target pencarian obat antimalaria adalah mekanisme penghambatan pembentukan hemozoin. Plasmodium dalam siklusnya akan mendegradasi hemoglobin menjadi heme bebas yang toksik untuk dirinya sendiri. Untuk melindungi dirinya parasit akan mengubah heme bebas menjadi hemozoin. Senyawa β-hematin merupakan analog senyawa hemozoin. Pada penelitian ini diuji aktivitas antimalaria terhadap 13 ekstrak etanol metode maserasi (dingin) dan 13 ekstrak air metode dekok (panas). Tanaman yang digunakan adalah salam koja (Murraya koenigii), akar kuning (Arcangelisia flava), jung rahab (Baeckea frutescens), pule (Alstonia scholaris), buah makassar (Brucea javanica), bidara (Ziziphus mauritiana), mimba (Azadirachta indica), murbei (Morus alba), tabat barito (Ficus deltoidea jack), ceplikan (Solidago virgaurea), srigunggu (Clerodendron serratum [L.] Sp r), rumput mutiara (Hedyotis corymbosa), bawang dayak (Eleutherine palmifolia). Pengujian menggunakan air panas dikarenakan cara masyarakat lokal dalam memanfaatkan tanaman obat akan dibandingkan dengan pelarut organik yang umum digunakan dalam penelitian. Ekstrak yang memiliki aktivitas terbaik diukur nilai IC50 dan dilanjutkan dengan skrining fitokimia. Hasil penelitian menunjukkan bahwa aktivitas antimalaria lebih baik menggunakan pelarut air dengan metode dekok dibandingkan pelarut etanol dengan metode maserasi. Ekstrak yang aktivitasnya paling tinggi adalah ekstrak air daun jung rahab sebesar 58,48% dengan nilai IC50 sebesar 51,5 ppm. Skrining fitokimia menunjukkan bahwa ekstrak air daun jung rahab mengandung golongan senyawa flavonoid, saponin dan tanin.
AKTIVITAS ANTIOKSIDAN KOMBINASI EKSTRAK ETANOL DAUN JARONG (Stachytarpheta indica) DAN BATANG CENTE (Lantana camara) Septiana, Eris; Mawadah, Nursifa; Simanjuntak, Partomuan
Media Farmasi: Jurnal Ilmu Farmasi Vol. 17 No. 2: September 2020
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v17i2.17299

Abstract

Penggunaan tanaman obat sebagai sumber senyawa antioksidan semakin meningkat. Beberapa tanaman yang telah dilaporkan sebagai sumber senyawa antioksidan adalah daun jarong (Stachytarpheta indica) dan batang cente (Lantana camara). Namun, belum ada penelitian yang melaporkan kombinasi kedua tanaman tersebut sebagai sumber antioksidan. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh kombinasi ekstrak etanol daun jarong dan batang cente terhadap aktivitas antioksidannya. Aktivitas antioksidan in vitro berdasarkan pada metode peredaman radikal bebas DPPH (2,2-diphenyl-1-picrylhydrazyl). Penapisan fitokimia dan analisis indeks kombinasi juga dilakukan untuk menentukan efek kombinasinya. Semua ekstrak etanol tunggal dan kombinasi menunjukkan aktivitas antioksidan. Kombinasi ekstrak etanol daun jarong dan batang cente dalam perbandingan 1:2 memberikan aktivitas antioksidan tertinggi dengan nilai IC50 sebesar 18,86 µg/mL. Penapisan fitokimia menunjukkan bahwa senyawa steroid, tanin, flavonoid, dan fenolik ditemukan pada kedua ekstrak. Alkaloid, kuinon, dan saponin hanya ditemukan dalam ekstrak daun jarong. Sementara itu, kumarin hanya ditemukan dalam ekstrak batang cente. Kombinasi ekstrak etanol menunjukkan efek sinergis ringan-sedang sampai mendekati aditif dengan nilai CI antara 0,7723-0,9607. Oleh karena itu, kombinasi ekstrak dapat meningkatkan aktivitas antioksidannya.
Co-Authors A.A. Ketut Agung Cahyawan W Amalia . ANDRIA AGUSTA Arif Soeksmanto Arini Khaerunnisa Batan, Alfrida Bina Lohita Sari Bustanussalam ., Bustanussalam Bustanussalam Bustanussalam, Bustanussalam Daniel Daniel Dian Malini Otovina, Dian Malini Dian Ratih Laksmitawati Djamil, Ratna Djoko, Wahyuni Dyna Grace Romatua Aruan E.G. Said, E.G. Edward J Dompeipen, Edward J Efendy, Oscar Enny Sudarmonowati ERIS SEPTIANA Esti Mumpuni, Esti Farida Farida Farida, Yunahara Faridah Faridah Fauzy Rachman Gianny, Demitra Hardiyati, Iin HARI PURNOMO Harmastini Sukiman, Harmastini Haro, Gindo Koraag, Alfa Michael Gabriel Kusharyoto, Wien Lamek Marpaung, Lamek Lekatompessy, Sylvia J.R. Lilik Sulastri M. Rahayuningsih Mastura, Mastura Mawadah, Nursifa Muhammad Ahkam Subroto Murniaty Simorangkir Nasution, Pandapotan Ngadiman Ngadiman NISA RACHMANIA MUBARIK Purwoko, Marcus Yudhi Putra, Ari Permana Reubun, Yonathan Tri Atmodjo Ribu Surbakti, Ribu Rudi Kartika, Rudi Rumella Simarmata, Rumella Rurianti, Wandesta Rutdianti, Rutdianti Saifur Rohman SARONOM SILABAN Setyningrum, Eka Damayanti Shirly Kumala Siswa Setyahadi Sri Hartati Sukiman, Harmastini I. Suwarno, Tisno Suwarno, Tisno Syamsudin Syamsudin Tarigan, Elsera Br. Tarso Rudiana, Tarso Taurhesia, Shelly Titi Parwati, Titi Tiwit Widowati, Tiwit Tonel Barus, Tonel Wiwi Winarti Yadi, Yadi Yatri Hapsari, Yatri Yudhi Purwoko, Marcus Laurentius