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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Journal of the Indonesian Tropical Animal Agriculture Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner To Maega: Jurnal Pengabdian Masyarakat Advances in Tropical Biodiversity and Environmental Sciences Journal of Veterinary and Animal Sciences
Luh Made Sudimartini
Laboratorium Farmakologi Dan Farmasi Veteriner, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Standarisasi Cemaran Mikroba Sampel Daun Pegagan sebagai Persyaratan Mutu Bahan Baku Sediaan Obat Luh Made Sudimartini; Genta Dhamara Adam Putranto; I Gusti Ketut Suarjana; I Made Merdana
Buletin Veteriner Udayana Vol. 14 No. 4 August 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.117 KB) | DOI: 10.24843/bulvet.2022.v14.i04.p01

Abstract

Pegagan is an excellent plant used as a raw material for traditional medicine. The bioactive content in pegagan leaves plays a role in the wound healing process, able to cure complex dermatitis in dogs, proven as neuroprotective, and can reduce oxidative stress of body cells. Many efficacies make pegagan leaves have the potential to be produced into various forms of medicinal preparation. This study will calculate the number of microbes’ contamination in pegagan leaves which will be used as various drug preparations and then compare it to the General Standard Parameters for Medical Plant Extracts of the Ministry of Health of Republic Indonesia in 2000 and Regulation of the Head of BPOM RI Number 32 of 2019. The examination of microbial contamination was carried out through the Total Plate Count and Yeast Fungus Test. The research data obtained were quantitative data that were analyzed by counting the number of microbes that grow on PCA and PDA after incubation at the appropriate growth temperatures. The results of this study show that the Total Plate Count from the sample was 3250 CFU/gr and the Yeast Fungus Test was 450 CFU/gr. The number of Total Plate Count has fulfilled the requirements for the Oral Drugs (£105 colony/gr) and required for Semi Solid Drugs (£107 colony/gr). The number of Yeast Fungus Test are required for Oral Drugs (£103 colony/gr) and required for Semi Solid Drugs (£104 colony/gr).
Kidney Histopathology of Wistar Rats Chronic Apical Periodontitis Luh Made Sudimartini; Anastasia Bhala; I Wayan Wirata; Ni Luh Eka Setiasih; Ni Kadek Eka Widiadnyani; I Made Merdana
Advances in Tropical Biodiversity and Environmental Sciences Vol 6 No 2 (2022): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2022.v06.i02.p04

Abstract

Chronic apical periodontitis is an inflammatory disease that causes periodontal tissue destruction and forms chronic lesions. This study aims to prove that the administration of green meniran leaf ethanol extract can affect the kidney histopathology of chronic apical periodontitis Wistar rats. This study used a randomized posttest only control group design with four treatment groups and twelve replications of three sub-groups according to the time of application of the medicament paste, namely the 7th, 14th, and 21st days so that 48 Wistar rats were used. The treatment group consisted of negative control (K1), only injected with disease-causing bacteria. The positive control group was given 2% Calcium Hydroxide and Chlorhexidine digluconate. Treatment group 1(P1) was given calcium hydroxide and 10% green meniran leaf ethanol extract, and treatment group 2(P2) was given calcium hydroxide, 2% chlorhexidine digluconate, and 10% green meniran leaf ethanol extract. All samples were male Wistar rats with a weight ranging from 300-350 grams. The variables observed were congestion and necrosis with a score of 0 (normal); 1 (focal); 2 (multifocal); 3 (diffuse). The data were analyzed using the Kruskal-Wallis non-parametric statistical test and if it showed a significant effect, it was continued with the Mann-Whitney test. The results of the Kruskal-Wallis test showed that in the K2, P1, and P2 treatments, there was a significant difference (P<0.05) in the category of congestion and necrosis of the histopathological changes of the kidneys. The results of this study indicate that the ethanol extract of green meniran leaves which has a function as an antioxidant can repair kidney damage caused by free radicals in chronic apical periodontitis conditions
Histopatologi Hati Tikus Wistar Periodontitis Apikalis Kronis Pasca Pemberian Medikamen Intrakanal Theresia Ene; I Made Merdana; Ida Bagus Oka Winaya; Ni Kadek Eka Widiadnyani; Luh Made Sudimartini
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p15

Abstract

Dental disease is a problem that is common in Indonesia, and it affects around 96.58% of the population. The use of chemical drugs that can cause side effects in patients makes people start other alternative treatments by using natural ingredients namely meniran (Phyllanthus niruri L). This study examines the liver histopathology of Wistar rats with chronic apical periodontitis after administration of intracanal drugs. This study looked at the effects of a medicament paste on rats for a period of seven, fourteen, and twenty-one days. Experimental animals were adapted to chronic apical periodontitis by inducing Enterococcus faecalis into right maxillary molars for 21 days.On day 22, all drug treatment groups were opened with GIC and given the prescribed drug treatment, and then closed again with GIC. The observed variables were the presence of inflammatory cell infiltration, hyperemia, and necrosis. Data were analyzed using a non-parametric statistical Kruskal-Wallis test, and if there was a significant difference, it was continued with the Mann-Whitney test. The results of the study showed that there was no significant difference between the three treatment groups (P1, P2, P3) and there was a significant difference between P0 and the other two groups. However, the results of statistical analysis of necrosis observed by histopathology in the liver showed that there was no significant difference between P0, P1, P2 and P3 treatments (P>0.05).From the research results, it can be concluded that administration of meniran leaves ethanolic extract can improve liver histopathology in rats with chronic apical periodontitis.
Efficacy of Polyherbal Oil Against Gastrointestinal Nematode in Kintamani Dogs I Made Merdana; Luh Made Sudimartini; Samsuri Samsuri; Putu Devi Jayanti
Journal of Veterinary and Animal Sciences Vol 4 No 2 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i02.p02

Abstract

Gastrointestinal helminth parasites in animals can be zoonotic-parasitic. This study aims to determine the efficacy of polyherbal oil formulations against intestinal worm parasites of Kintamani dogs. This study used 24 Kintamani Dogs aged 3-6 months which were kept by releasing them into the wild. The anthelmintic potency of polyherbal oil formulations was determined by measuring Eggs Per Gram of feces (EPG), using the Modified Stoll Dilution method before and seven days after treatment. P0 as control was given a placebo, while groups P1, P2, and P3 were given Polyherbal oil at doses of 300, 600, and 900 mg/kg body weight for three consecutive days. The results showed that there was a decrease in the EPG value sequentially P0, P1, P2, and P3 which was -4%, 23.1%, 66.7%, and 81.3%. Based on the identification of worm eggs, it was shown that the efficacy of polyherbal oil against Toxocara canis decreased EPG at P1, P2, and P3 by 26.09%, 73.33%, and 86.08%. Against Ancylostomata spp respectively at P1, P2 and P3 caused a decrease in EPG of 17.39%, 52.63%, and 72.0%. Against Strongyl spp worms respectively at P1, P2 and P3 caused a decrease in EPG by 16.67%, 58.82%, and 73.68%. Statistical analysis showed that polyherbal oil had a significant effect (P<0.05) on the decrease in EPG. We can conclude that the polyherbal oil formulation has vermicidal efficacy against intestinal worm parasites in Kintamani dog puppies.
Herbal Extract as An Antibacterial Against Gram Positive Bacteria Causing Dermatitis Complex I Nyoman Suartha; I GustiKetut Suarjana; Luh Made Sudimartini; I Made Merdana; I Made Dira Swantara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p07

Abstract

Study in order to evaluate the antibacterial effect of herbal extract from leaves of three different species of plant namely neem(Azadirachtaindica A. Juss), gotu-kola (Centellaasiatica), andsoursop (Annona muricata L) against bacteria causing dermatitis complex in dogs have been undertaken. The antibacterial activities of the herbal extract were evaluated using the agar diffusion test with extract at three different concentrations 5%, 10%, and 25%, respectively.Staphylococcus aureus was isolated from the dermatitis complex cases in dogs. The results showed various antibacterial activities of all the herbal extract at all concentration as indicated by the diameter of inhibition haloes produced around the orifice. The average inhibition haloes for each herbal extract concentration was 2.19± 0.53 mm; 4.32± 0.84 mm; 5.86± 0.79 mm, respectively. It can be concluded that herbal extract from the three plants is a potential antimicrobial against agents causing dermatitis complex.
Sonogram Testicular Relationships with Semen Quality of Kintamani Dog I Wayan Nico Fajar Gunawan; Luh Made Sudimartini; I Wayan Wirata
Journal of Veterinary and Animal Sciences Vol 4 No 1 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i01.p03

Abstract

Ultrasonography is a high-end tool whose initial use to diagnose pregnancy and now being used for fetal development monitoring, birth prediction, diagnosis, and management of reproductive diseases. The aim of this study was to evaluate the initial relationship between body weight, testicular sonogram on the quality of semen collected from the basal Kintamani dogs which is expected to be useful in reproductive management of Kintamani dogs. The study used 10 male Kintamani dogs with healthy reproductive status. The tool used is in the form of an ultrasound for livestock, namely the Veterinary Ultrasound Scanner with a 5 MHz probe transducer, the image mode in the form of B-mode which will be displayed on the LCD with a size of 10 inches. Ultrasound examination is carried out in series every once a week. The semen examination was carried out immediately after the testicular ultasonography examination was carried out. No association was found between echogenicity and heterogeneity of the prostate gland or other size of the prostate gland and semen quality. Testicular volume, prostart and age did not affect the quality of Kintamani dog spermatozoa, while the testicular sonogram had an effect on the quality of Kintamani dog spermatozoa.
Sebaceous Adenoma in a Geriatric Poodle Dog: A Case Report Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah; Sudimartini, Luh Made; Merdana, I Made; Sudipa, Putu Henrywaesa; Pemayun, I Gusti Agung Gde Putra; Sewoyo, Palagan Senopati
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.413-419

Abstract

A 14-year-old black male Poodle was brought to the Animal Teaching Hospital, Udayana University by its owner with clinical signs of frequent licking of its left front paw. Upon examination, red bumps were observed on the left front leg, accompanied by small, round black spots scattered on the dorsal side of the body. Additionally, black nodules were present on the lower eyelids and hind limbs. Surgical intervention was undertaken to excise the tumor mass, with the animal under anesthesia induced by ketamine at 5 mg/kg BW intravenously. The reddish nodule was excised by performing an elliptical incision at the base of tumor. Postoperatively, the animal received an antibacterial injection comprising ceftriaxone and tazobactam at 25 mg/kg BW intramuscularly and antiseptic wound dressing for supportive care. Microscopic examination revealed neoplastic cells arranged into lobules of varying sizes and shapes within the tumor mass. These lobules consisted of differentiated sebocytes and basaloid cells. At the periphery of the neoplastic lobules, the basaloid cells displayed several layers and exhibited invasion with moderate anisocytosis. The mitotic index was no more than ten cells in one field of view. Based on these histopathological features, the tumor was confirmed to be a sebaceous adenoma. After a 10-month follow-up period, there were no signs of tumor recurrence observed.
Optimalisasi Strategi Hilirisasi dan Komersialisasi Produk Inovasi di Universitas Udayana Desak Nyoman Dewi Indira Laksmi; Anak Agung Istri Sri Wiadnyani; Luh Made Sudimartini
To Maega : Jurnal Pengabdian Masyarakat Vol 7, No 2 (2024): Juni 2024
Publisher : Universitas Andi Djemma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35914/tomaega.v7i2.2556

Abstract

Pengabdian ini membahas program percepatan hilirisasi dan komersialisasi produk inovasi di lingkungan Universitas Udayana. Fokus utama penelitian adalah melibatkan lembaga inkubator bisnis sebagai fasilitator utama dalam mengakselerasi produk inovasi dari dosen-dosen di Universitas Udayana. Dengan mengusung model bisnis inkubasi yang melibatkan tahapan sosialisasi, pra inkubasi, inkubasi, dan demo day, penelitian ini menciptakan roadmap pengabdian yang memberikan arah pengembangan bisnis dari hasil riset menjadi produk komersial. Melalui kegiatan pelatihan, seperti semangat berbisnis, pemahaman legalitas usaha, dan strategi pengembangan usaha, serta sosialisasi program Pendanaan Riset dan Inovasi untuk Indonesia Maju - Perusahaan Pemula Berbasis Riset (RIIM-PPBR), penelitian ini bertujuan untuk memotivasi dosen di Universitas Udayana untuk menghasilkan produk inovasi dan membentuk start-up. Dengan sasaran pada dosen yang telah memiliki produk inovasi atau yang masih dalam tahap pengembangan, program ini berusaha meningkatkan kualitas produk inovasi yang dapat dihasilkan oleh dosen di lingkungan Universitas Udayana. Simpulan penelitian menekankan pentingnya kolaborasi antara akademisi, pemerintah, dan industri untuk mempercepat hilirisasi produk inovasi, sementara saran diajukan berfokus pada intensifikasi kolaborasi melalui berbagai event kegiatan guna meningkatkan jumlah produk inovasi yang dapat dikomersialkan dan dimanfaatkan oleh masyarakat.
Identification and Prevalence of Aspergillus sp Isolated from Bali Dog’s Skin Sudipa, Putu Henrywaesa; Puja, I Ketut; Dharmayuda, Anak Agung Gde Oka; Gunawan, I Wayan Nico Fajar; Sudimartini, Luh Made; Jayanti, Putu Devi; Sukernayasa, I Wayan; Mufa, Romy Muhammad Dary
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21594

Abstract

Aspergillus sp are fungi that commonly caused a systemic respiratory infection in Human and Dog. In Bali, most of the dog are free-roaming dogs, and has poor skin health condition. Some of them are suspected with fungal infection. Aspergillus sp has high risk to infect the dog, because they are available everywhere in environment. Therefore, this research is conducted for adding primary data of Aspergillus sp isolated from dog’s skin and their prevalence. The samples are 15 free-roaming Bali dogs with suspected fungal infection. Identification was carried out by using microscopic and macroscopic observation methods, skin swab was taken from the dog’s skin using the Mackenzie method, and the sterile toothbrush swab gently from the dog’s skin to the Sabouraud Dextrose Agar (SDA) media. The fungi were cultured on SDA media for macroscopic identification for approximately 3-4 days, mostly to observe how the fungal colony grew and its color. Microscopic identification are using adhesive tape, the tape was gently pressed into the fungal colony, the tape was stuck into the surface of object-glass with 3-4 drops of Methylene Blue staining, and the object glass was examined by using a light microscope, the findings of Aspergillus sp fungi are recorded and the data are descriptively presented. The result shows that most Aspergillus species that were found is Aspergillus fumigatus and Aspergillus niger according to their colonies growth and microscopic appearance, and the prevalence from 15 samples were 53.33%. Keywords: Aspergillus sp, fungi, Bali Dogs, Identification, Prevalence
Co-Authors Afrizal Choirul Umam Ainul - Hidayah Amelia Avianti Saritjang Anak Agung Ayu Mirah Adi Anak Agung Gde Jaya Wardhita Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayuda Anak Agung Gde Oka Dharmayudha Anak Agung Istri Sri Wiadnyani Anak Agung Sagung Kendran Anastasia Bhala Archie Leander Maslim Boro, Saptarima Eka Estiani Cahyaniarta, I Kade Candra Cyrilus Jefferson Bour Desak Nyoman Dewi Indira Laksmi Elsa Hidayati Elti Febilani Fernandes, Nuno Firdaus, Ihsanul Genta Dhamara Adam Putranto Gunawan, I Wayan Nico Fajar Gunawan, I. W. N. F. Heparandita, Ananda Agung Dextra I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Gusti Putu Tovan Mahottama I GustiKetut Suarjana I Ketut Anom Dada I Ketut Berata I Ketut Puja I Made Agus Wiranata I Made Dira Swantara I Made Kardena I Made Merdana I Made Sukada I Nyoman Suartha I W. F. Gunawan I Wayan Fandhi Wibawa Lostapa I Wayan Gorda I Wayan Juli Sumadi I Wayan Sudira I Wayan Wirata I Wayan Wirata Ida Bagus Dimas Kusumadarma Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Iwan Harjono Utama Jayawardhita, Anak Agung Gde K. K. Agustina Kadek Karang Agustina Ketut Budiasa Komang Sri Gilang Airlangga Laksmi, D. N. D. I Luh Dewi Anggreni Luwis, Jeremy Christian Made Suma Anthara Made Suma Anthara Megariyanthi, Ni Putu Arie Mufa, Romy Muhammad Dary Ni Kadek Eka Widiadnyani Ni Kadek Laura Sastrawan, Ni Kadek Laura Ni Luh Eka Setiasih Ni Luh Putu Kusuma Clara Dewinda Ni Made Rita Krisna Dewi Ni Nyoman Werdi Susari Ni Putu Ayu Dewi Wijayanti Ni Putu Trisna Asih Pratama Putra, I Wayan Dwika Putra, Komang Darma Yudha Putrawan, Baja Sadhayu Putri, Adelia Putri, Ayu Chitra Adhitya Putu Adrian Junaedi Putu Dessy Wilantari Putu Devi Jayanti Putu Henrywaesa Sudipa Putu Oka Samirana Putu Suastika Rahim, M. Andry Rohmandhani, Roby Rosni Lumban Gaol Sakura, Yoakim Wilson Samsuri , Samsuri Samsuri Samsuri Samsuri Saweng, Cikal Farah Irian Jati Sawitajaya, I Made Sewoyo, Palagan Senopati Sitohang, Martina Tiodora Steven Dwi Purbantoro Sukernayasa, I Wayan Sutadisastra, Nisha Aisya Theresia Ene Wayan Herry Gumawan Widyanti, Agnes Indah Wirawan, I Gede Yoana Pratiwi Clara Pakpahan