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Potensi Probiotik Terhadap Feed Efficiency dan Egg Mass pada Ayam Petelur Mas'ad, Khasmawati; Lokapirnasari, Widya Paramita; Al Arif, Moh. Anam; Soeharsono, Soeharsono; Kurnijasanti, Rochmah; Harijani, Nenny
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.203-207

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui penggunaan probiotik yang mengandung Enterobacter sp., Cellulomonas sp., Actinomyces sp., Bacillus sp., dan Lactobacillus sp. terhadap feed efficiency dan egg mass pada Ayam Petelur. Sebanyak 36 ekor ayam petelur umur 30 minggu yang terdiri dari 3 perlakuan.  Perlakuan terdiri dari P0 (tanpa probiotik), P1 (probiotik 1 ml/liter), P2 (probiotik 2 ml/liter). Hasil penelitian pemberian probiotik berpengaruh (p<0.05) terhadap feed efficiency dan egg mass. Pemberian probiotik sebanyak 2 ml/liter air minum (P2) menunjukkan nilai feed efficiency tertinggi (66.1b ± 3.29). Nilai egg mass tertinggi (51.9b ± 3.36) terdapat pada P1 yaitu perlakuan yang diberi probiotik sebanyak 1 ml/liter air minum. Dapat disimpulkan bahwa pemberian probiotik dengan dosis 1 ml/liter (P1) maupun dosis 2 ml/liter (P2) meningkatkan feed efficiency dan egg mass.
Effect of Probiotics Bacillus subtilis on Feed Efficiency and Egg Mass of Laying Hens Pratama, Hanif Sabekti; Lokapirnasari, Widya Paramita; Soeharsono, Soeharsono; Al-Arif, Muhammad Anam; Harijani, Nenny; Hidanah, Sri
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.37-41

Abstract

The aim of this research was to investigate the effect of probiotics Bacillus subtilis on feed efficiency and egg mass of laying hens. This research was using 36 of 38 weeks-old laying hens Isa Brown strain. The concentration of probiotics B. subtilis was 1 x 109 CFU/ml, there were three kinds of treatments, P0 (without given probiotics B. subtilis), P1 (given 0.1 gram of probiotics B. subtilis per kg of feed), and P2 (given 0.2 gram of probiotic B. subtilis per kg of feed). The results of feed efficiency, the provision of probiotics can provide a significantly different values (p <0.05) between treatments either P0, P1, or P2. Provision probiotics P1 showed the highest value of feed efficiency (81.72 ± 5.28), while the lowest value at P0 was (64.06 ± 3.30). The results showed that there were significantly different in egg mass values (p <0.05) between treatments both P0, P1, and P2. Probiotic P2 shows the highest value (53.30 ± 2.13), while the lowest egg mass value of probiotics is as much as P1 (40.83 ± 4.73). Based on the results of this study it can be concluded that the B. subtilis probiotics both at a dose of 0.1 gram / kg of feed and a dose of 0.2 gram / kg of feed can affect the growth performance of laying hens.
Bacillus subtilis Probiotics in Chicken Feed Improve Egg Quality with Differences in Shelf Life Wardiana, Nurul Ika; Lokapirnasari, Widya Paramita; Harijani, Nenny; Al-Arif, Mohammad Anam; Ardianto, Ardianto
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.8-13

Abstract

This study aims to examine the effect of giving Bacillus subtilis probiotics in laying hens on improving the quality of internal and external eggs with differences in shelf life. A total of 60 laying hens were divided into 4 treatment groups, namely P0 as a control that was not given probiotic B. Subtilis, P1 feed was added 0.005 grams B. Subtilis, P2 feed with added 0.01 grams B. Subtilis, P3 feed added 0.02 grams B. Subtilis then the eggs produced are stored for 1 day, 5 days and 10 days. The results of this study indicate that the administration of B. Subtilis probiotics with a concentration of 0.02 grams affects the increase in egg weight, egg yolk index value and HU values. The addition of B. Subtilis probiotics to racial feed can improve egg quality.
Antibacterial of Cinnamon Bark (Cinnamomum burmannii) Essential Oil Against Methicillin-Resistant Staphylococcus aureus Fadlilah, Shinta Levea Ni'matul; Effendi, Mustofa Helmi; Tyasningsih, Wiwiek; Suwanti, Lucia Tri; Rahmahani, Jola; Harijani, Nenny; Ramandinianto, Sancaka Chasyer; Khairullah, Aswin Rafif
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.56-62

Abstract

This study aimed to determine the in vitro antibacterial activities of essential oil from cinnamon bark (Cinnamomum burmannii) on Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk. Essential oil from cinnamon bark obtained from the steam distillation method and essential oil was made in a series dilution with a concentration of 1%, 2%, 4%, and 8%. The antibacterial activities were tested using the disk diffusion method. Results showed from five isolates of MRSA, one isolate was sensitive of essential oil with a concentration 2% and all of the isolate was sensitive with a concentration 4% and 8%. The antibacterial activity showed by inhibition zones on  MRSA. The results suggest that the activity of the essential oils of cinnamon bark has an antibacterial effect on MRSA and it is developed as phytopharmaca.
Detection of Escherichia coli Contamination using Most Probable Number (MPN) methods in Chicken Meats in Market of Surabaya Wardhana, Dhandy Koesoemo; Safitri, Devi Ayu; Annisa, Suwaibatul; Effendi, Mustofa Helmi; Harijani, Nenny
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.118-124

Abstract

Foodborne diseases are diseases transmitted through contaminated food and are one of the main public health problems. The bacteria that usually contaminates chicken meat is Escherichia coli. E. coli contamination in chicken meat can affect consumer health. Chicken meat processing and market conditions affect the level of bacteria contamination. This study aims to measure the contamination of E. coli bacteria in chicken meat in Surabaya's markets. The method which used to count and estimate the number of E. coli bacteria found in chicken meat in this study was Most Probable Number (MPN). The results showed 20 samples (33.3%) from 60 samples gave negative results. This indicated that only 20 samples of chicken meat were safe for consumption because the MPN value is <1 í— 101 CFU/g while the remaining 40 samples (66.7%) were not safe for consumption because the MPN value is> 1 í— 101 CFU/g. The standard of SNI 3924: 2009 concerning the minimum requirement for the amount of E. coli contamination in chicken meat is 1í—101 CFU/g so that the contamination of E. coli which found in chicken meat in Surabaya was high.
Correlation of Reproduction Management on Losses of Dairy Farmers in Wagir District, Malang Regency Ishaq, Rizal Maulana; Suharsono, Suharsono; Harijani, Nenny; Hidanah, Sri; Mustofa, Imam
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.281-284

Abstract

This study was conducted to find out how much losses the breeders get when their cattle experience a decline in day open (DO), service per conception (S/C), and calving interval (CI).There were 108 lactating dairy cows from the dairy farm, Wagir District, Malang Regency.The data that has been taken is then processed by analysis using linear regression method.The results of the analysis prove that reproductive management has an effect of 82% on farmer expenditure.For each setback DO per day, the farmer pays Rp. 609,05.Meanwhile, for each additional S/C the farmer pays Rp. 99,921.7.For each setback the farmer CI will incur a fee of IDR 228,54 / day.So that the equation found is expendses = 609,05 (DO) + 99,921.7 (s / c) +228,54 (CI).
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Most Probable Number of Coliform on Beef from Krian Abattoir, Sidoarjo Pratiwi, Sambang Dewi; Harijani, Nenny; Sarudji, Suryanie; Budiarto, Budiarto; Estoepangestie, Soelih
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36476

Abstract

The problem on food health is the high amount of bacterial contamination of food served by food industries. The quality of beef can be affected by the level of bacterial contamination in meat. Coliform is a group of bacteria used as a sanitary hygiene indicator. The purpose of this study is to measure the value of MPN Coliform in beef at Krian's abattoir of district Sidoarjo. Thirty samples were taken from the abattoir of Krian, Sidoarjo. Most Probable Number was used to determine the level of Coliform's contaminate beef. The results showed that all beef samples at abattoir were contaminated with Coliform. The average contaminant levels was 706,76 MPN/g. Twenty six samples exceed the maximum limit of Coliform bacteria contamination in accordance with Indonesian National Standard (SNI). The highest Coliform MPN value is ≥ 1600 MPN / g, whereas the lowest MPN Coliform value is 8 MPN/g.
ANALISIS FAKTOR YANG MEMPENGARUHI KEJADIAN MASTITIS SUBKLINIS DAN KLINIS PADA SAPI PERAH (STUDI KASUS DI KOPERASI AGRIBISNIS DANA MULYA KECAMATAN PACET, KABUPATEN MOJOKERTO) Nisa, Hefi Choirun; Purnomo S., Bambang; Damayanti L., Tita; Hariadi, Mas'ud; Sidik, Romziah; Harijani, Nenny
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.789 KB) | DOI: 10.20473/ovz.v8i1.2019.66-70

Abstract

The study was conducted to know the factor that potentially affect the incidence of subclinical and clinical mastitis in dairy cows in the area Agribusiness Cooperative Dana Mulya Pacet, Mojokerto. Data retrieval were done by examining the incidence of subclinical and clinical mastitis in 100 lactation dairy cows. Milk samples were taken from milking each quarter of dairy cows in the afternoon, then tested using CMT reagent (California Mastitis Test). In addition data was obtained from farmers through structured questionnaires. The data was processed and presented in tabular form and analyzed statistically by the method of regression trees. The conclusion of this research showed that dairy cows in the area of Agribusiness Cooperative Dana Mulya Pacet, Mojokerto affected by subclinical mastitis reached 76% and clinical mastitis 11%. This was influenced by the milking sequence factor of the nipple which was not distinguish between sick and healthy nipples, the lack of sanitation before milking, and the location of livestock disposal is too close to the cowshed.
Penggunaan kombinasi Gonadotropin untuk pengobatan hipofungsi ovarium pada sapi perah Masruro, Novia Alfi; Mulyati, Sri; Madyawati, Sri Pantja; Samik, Abdul; Ratnani, Hermin; Harijani, Nenny
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (662.863 KB) | DOI: 10.20473/ovz.v9i1.2020.23-27

Abstract

This case report aims to determine the response of estrus and pregnancy rates in dairy cows with ovarian hypofunction by administering a combination of gonadotropin hormones. The sample used was ten dairy cows that had been calving, were not pregnant, showed no signs of estrus for more than 60 days after the last calving and had ovarian hypofunction based on rectal palpation. All dairy cows were injected 300 IU PG-600, then divided into two groups, each injected with 100 and 300 IU hCG respectively for first group and second group, intramuscularly at the time of insemination. The results showed that the estrus response occurred in all cows, in 6-8 (6.8 ± 0.84) days (first group), and 6-7 (6.2 ± 0.45) days (second group). Trans-rectal palpation pregnancy diagnosis 60 days after artificial insemination showed that all dairy cows in both groups were pregnant. It could be concluded that the treatment of dairy cows suffering from ovarian hypofunction with a combination of 300 IU PG-600 and 100 IU hCG was sufficient to induce 100% estrus rates and 100% pregnancy rates.
Co-Authors A. T. Soelih Estoepangestie Abdul Samik, Abdul Agnes Theresia Soelih Estoepangestie Al Arif, M. Anam Al Arif, Moh. Anam Al arif, Mohammad Anam Al-Arif, M. Anam Al-Arif, Muhammad Anam Anam Al Arif Anam Al Arif, Mohammad Andriani, Anita Dwi Annisa, Suwaibatul Budiarto Budiarto Caska - Chusniati, Sri Damayanti L., Tita Dhimar Maulud Dyahningrum Didik Handijatno Elok Sukma Pertiwi Endang Suprihati Estoepangestie, Soelih Fadlilah, Shinta Levea Ni'matul Fedik Abdul Rantam Fithria Nisa Hanifah Hermin Ratnani Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Irawan, Putri Indra Ishaq, Rizal Maulana Iwan Sahrial Hamid Karimah, Balqis Khairullah, Aswin Rafif Koesnoto Soepranianondo Lailatul Maghfiroh Lucia Tri Suwanti, Lucia Tri Mardian, Nur Zaini Nanda Mariana Ruth Theresia Hutabarat Mas'ad, Khasmawati Masruro, Novia Alfi Mas’ud Hariadi Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nanda, Elga Rastika Virya Nining Sari Virgandina Vinola Nisa, Hefi Choirun Poedji Hastutiek Pratama, Hanif Sabekti Pratiwi, Sambang Dewi Purnomo S., Bambang Raharjo, Dadik Rahmahani, Jola Rahmi, Chintyadhevi Aulia Ramandinianto, Sancaka Chasyer Rochmah Kurnijasanti Romziah Sidik, Romziah Safitri, Devi Ayu Sarudji, Suryanie Setiawan Koesdarto Siti Hadijah Soeharsono Soeharsono Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati, Sri Pantja Subagyo, Safira Fauziyyah Suharsono Suharsono Sunarso, Agus Suwarno Suwarno Tri Nurhajati Trilas Sardjito Tyasningsih, Wiwiek Wardhana, Dhandy Koesoemo Wardiana, Nurul Ika Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yunita, Maya Nurwartanti