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All Journal Buletin Peternakan Jurnal Veteriner Jurnal Ilmu dan Teknologi Hasil Ternak Buletin Plasma Nutfah Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika AGRIVITA, Journal of Agricultural Science JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production Jurnal Ternak Jurnal Agriovet Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Livestock and Animal Research Jurnal Agripet Animal Production Journal of Agriprecision & Social Impact
Tri Eko Susilorini
Faculty Of Animal Science, Brawijaya University, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Buletin Peternakan

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Polymorphism of Cyt-b Gene in Several Indonesian Cattle Using PCR-RFLP Method Agung, Paskah Partogi; Perwitasari, Dyah; Farajallah, Achmad; Said, Syahruddin; Kaiin, Ekayanti Mulyawati; Saputra, Ferdy; Hermansyah, Alfandy; Kuswati, Kuswati; Susilorini, Tri Eko; Zein, Moch. Syamsul Arifin
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.87484

Abstract

The cytochrome b (Cyt-b) gene is one of the genes that is located in the mitochondrial DNA. Variations in the Cyt-b gene can be used to compare different animal species to investigate the origin of certain animal species. This study aimed to assess the genetic diversity of Indonesian local cattle breeds, including Bali cattle as an Indonesian native cattle breed and Banteng as the wild type of Bali cattle, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 336 individual DNA samples from Indonesian cattle breeds were analyzed in this study. The RFLP method using three restriction enzymes, i.e., HinfI, HaeIII, or XbaI, was used to identify the variation of the Cyt-b gene. The Cyt-b gene was polymorphic based on the PCR-RFLP method. There were six alleles of the Cyt-b gene found in this study, i.e., A and B allele (HinfI), C and D allele (HaeIII), X and Y allele (XbaI). All alleles can be found in Pasundan, Madura, and PO cattle. Pesisir cattle have the highest allele frequency D. The UPGMA results showed three clusters of Indonesian native cattle in this study. Cluster 1 consists of Pasundan, Banteng, and Bali cattle. Cluster 2 consists of Madura, PO, and SO cattle. Pesisir was separated from other cattle in cluster 3. The X allele could become an indicator to distinguish Banteng and Bali cattle.
Co-Authors Abdul Manab ACHMAD FARAJALLAH Adelina Ari Hamiyanti Aditya Cahya Wardhana Adli, Danung Nur Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Zarkasi Efendi Ali Murthadho Amam Amam Amam Amam Angel Aprilia Panjaitan Anggun Permata Sari Anif Mukaromah Wati Anisa Wulandari Ardiantoro, Ari Aswah Ridhowi Desinta Wulandari Desinta Wulandari Dyah Perwitasari Efendi, Ahmad Zarkasi Endang Setyowati Ferdy Saputra Firman Jaya Fitria Ella Wardani Fitria Ella Wardani Gani, Tifany Sonia Gatot Ciptadi Guruh Prasetyo Hanum Muarifa Hanum Muarifah Heri Damayanti Hermansyah, Alfandy Husnassabil, Ahmad Hadin Ike Mawarni Handayani Ilmiatus Sholichatunnisa Imam Thohari Inggita Leli Murtika Inggita Leli Murtika K Ghozi Kaiin, Ekayanti Mulyawati Kanzul Kamal Putra Kusumastuti, Anie Eka Kuswati Kuswati Kuswati Kuswati Larasati, Rizka Latifah Hanum Lutfiana Safitri Manik Eirry Sawitri Marjuki Marjuki Masdiana Ch. Padaga Mashudi Mashudi Mashudi Mashudi Moch Sasmito Djati Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Muhammad Halim Natsir Muhammad Sanjaya Kusuma Nanda Dwi Putra Nur Hidayah Nur Hidayah Nurgiartiningsih, V. M. A. Nurul Huda, Asri Oki Selfiana Marsono Osfar Sjofjan Palayukan, Jisril Paskah Partogi Agung Paskah Partogi Agung Pramujo, Muhammad Prasetyo, Guruh Puguh Surjowardojo Puguh Surjowardojo Puguh Surjowardojo Putri, Aprillia Ika Rachmad Dharmawan Ramdhan Riky Winandi Rifa'i Rifa'i Rifa’i Rifa’i Rini Dwi Wahyuni Rini Dwi Wahyuni Septian, Wike Andre Septian Sucik Maylinda Sucik Maylinda Sucik Maylinda Surjowadojo, Puguh Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Syahruddin Said Tasya Nur Karina Teguh Wicaksono Umi Wisaptiningsih Vasco Benarivo Widi Nugroho Wike Andre Septian Wike andre Septian Wina Astriyani Yuli Arif Tribudi