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PENGEMASAN VAKUM PADA GURAMI PRESTO UNTUK MENINGKATKAN KUALITAS PRODUK DI KOPERASI PRODUKSI AMANAH SEJATI KOTA BENGKULU Ulfah Anis; Mimi Sutrawati
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 5, No 6 (2022): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v5i6.2303-2308

Abstract

Gourami fish has been cultivated and marketed by the Amanah Sejati cooperative located in Bengkulu city. Gurami is sold as fresh product. The price is not comparable with the cost of gurami cultivation. Therefore, it is necessary to make an effort to increase the economic value of the gourami fish. One of the solutions is to make it into presto gourami products. The gourami fish is then vacuum-packed to improve the quality and extend its shelf life. Community service aims to (1) provide knowledge about the type of packages, and (2) provide knowledge about how to vacuum packaging on presto gourami. These community services are coordination and socialization, counseling, and practice. These services were going well. The participants were enthusiastic about the counseling and practice.  
Deteksi dan Identifikasi Begomovirus pada Tembakau yang Ditanam Tumpang Sari dengan Tanaman Cabai Sutrawati, Mimi; Nadrawati, Nadrawati; Djamilah, Djamilah; Aulia, Ewa
Agrikultura Vol 34, No 3 (2023): Desember, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i3.47941

Abstract

Beragam spesies Begomovirus telah dilaporkan di berbagai daerah di Indonesia. Gejala penyakit mosaik menguning akibat infeksi Begomovirus telah dilaporkan pada berbagai tanaman budidaya di Bengkulu antara lain cabai, mentimun, labu, melon, pepaya, dan gulma. Tanaman tembakau banyak ditanam sebagai tanaman pinggir di lahan budidaya cabai. Berdasarkan pengamatan di lahan budidaya cabai di Kabupaten Kepahiang, Bengkulu ditemukan tembakau bergejala keriting dan mosaik kuning. Tujuan penelitian ini adalah mendeteksi dan mengidentifikasi Begomovirus penyebab penyakit mosaik menguning tembakau di Bengkulu. Deteksi dan identifikasi virus dilakukan dengan menggunakan metode polymerase chain reaction (PCR) menggunakan primer universal yang mengkode transcriptional activator protein (TrAp) dan replication-associated protein (Rep) dengan target fragmen ± 900 pb. Hasil amplifikasi PCR menunjukkan adanya pita target ± 900 pb pada sampel daun tembakau. Produk PCR kemudian dikirim ke First Base, Malaysia untuk dilakukan sikuensing. Begomovirus yang menginfeksi tembakau  menunjukkan homologi 99% dengan isolat Pepper Yellow Leaf Curl Indonesia Virus (PYLCIV) pada cabai di Bali (nomor aksesi LC381263). Hasil identifikasi ini merupakan laporan pertama infeksi PYLCV pada tanaman tembakau di Bengkulu.
Pemanfaatan Limbah Sayuran dan Buah untuk Pembuatan Ekoenzim di Desa Lawang Agung Kabupaten Seluma, Provinsi Bengkulu Sutrawati, Mimi; Romeida, Atra; Anwar, Guswarni
DHARMA RAFLESIA Vol 22 No 1 (2024): JUNI (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v22i1.35995

Abstract

Limbah buah dapat dimanfaatkan sebagai bahan baku pembuatan ekoenzim. Selama ini limbah buah biasanya hanya dimanfaatkan sebagai bahan baku kompos, padahal banyak cara pemanfaatan limbah yang dapat menghasilkan beragam produk untuk keperluan sehari-hari. Tujuan kegiatan pengabdian ini untuk menyebarkan teknik sederhana pemanfaatan limbah rumah tangga yaitu fermentasi limbah buah menjadi ekoenzim. Kegiatan Pengabdian pada Masyarakat (PPM) ini dilakukan oleh tim Kewirausahaan Fakultas Pertanian Universitas Bengkulu. Kegiatan diawali dengan penjelasan materi tentang ecoenzim, pemanfaatannya dalam kehidupan sehari-hari dan praktek pembuatan ekoenzim skala rumah tangga. Evaluasi menunjukkan peningkatan pengetahuan masyarakat tentang ekoenzim. Peserta umumnya tertarik menerapkan teknologi pembuatan ekoenzim untuk pengolahan limbah buah di rumah masing-masing.
Infection of Ageratum yellow vein virus on Weed Crassocephalum crepidioides in Bengkulu Marpaung, Nia Kurniati Br.; Sutrawati, Mimi; Ganefianti, Dwi Wahyuni; Novanda, Ridha Rizki; Pamekas, Tunjung
Jurnal Fitopatologi Indonesia Vol 19 No 1 (2023): Januari 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.1.39-44

Abstract

Beberapa jenis gulma dengan gejala infeksi virus ditemukan pada tiga sentra pertanaman pepaya (Carica papaya) di Provinsi Bengkulu, Indonesia. Gejala pada gulma ialah tulang daun dan lamina daun menguning, mosaik kuning, dan keriting. Gejala tersebut mirip dengan gejala infeksi Begomovirus pada beberapa jenis tanaman. Penelitian dilakukan dengan tujuan mendeteksi dan mengidentifikasi spesies Begomovirus pada spesies gulma Crassocephalum crepidioide. Deteksi Begomovirus dilakukan dengan metode polymerase chain reaction menggunakan sepasang primer universal Begomovirus, SPG1/SPG2. Pita DNA berukuran 912 pb berhasil diamplifikasi dari sampel gulma C. crepidioides dengan gejala daun keriting. Berdasarkan analisis Blastn, sampel Begomovirus asal gulma C. crepidioides memiliki kekerabatan yang paling dekat dengan Ageratum yellow vein virus (AYVV) isolat asal Taiwan (DQ866134.1) dengan homologi sebesar 99%. Hasil penelitian ini merupakan laporan pertama infeksi AYVV pada C. crepidioides di Indonesia.
Begomovirus Associated with Yellow Mosaic Symptom on Eggplant Plant in Bengkulu: Begomovirus Associated with Yellow Mosaic Symptom on Eggplant Plant in Bengkulu Nabillah, Ade; Alamria, Intan Suryani; Sari, Icha Kurnia Nanda; sutrawati, mimi; Bustamam, Hendri
Jurnal Fitopatologi Indonesia Vol. 20 No. 1 (2024): Januari 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.1.47-53

Abstract

Terung (Solanum melongena) merupakan tanaman hortikultura yang memiliki nilai ekonomi tinggi dan dibudidayakan secara luas. Salah satu kendala budi daya tanaman terung ialah adanya infeksi virus yang menyebabkan penyakit. Pada beberapa pertanaman terung di Bengkulu ditemukan gejala sistemik mosaik kuning yang mirip dengan infeksi Begomovirus pada cabai. Penelitian bertujuan mendeteksi dan mengidentifikasi penyebab gejala mosaik kuning pada tanaman terung di Bengkulu yang diduga disebabkan oleh Begomovirus dan berasosiasi dengan betasatelit. Sampel diambil secara purposive dari pertanaman terung di Kota Bengkulu, Kabupaten Bengkulu Tengah, Kabupaten Bengkulu Utara, Kabupaten Kepahiang, dan Kabupaten Rejang Lebong. Deteksi virus dilakukan dengan metode PCR menggunakan primer universal Begomovirus dan primer spesifik betasatelit serta identitas virus dikonfirmasi dengan perunutan sikuen DNA. PCR dengan primer universal Begomovirus dan betasatelit berhasil mengamplifikasi DNA berturut-turut berukuran ±912 pb dan ±1300 pb dari sampel daun yang bergejala mosaik kuning, malformasi daun, dan penebalan tulang daun. Berdasarkan hasil perunutan DNA sampel dari Bengkulu Utara dan Kepahiang menunjukkan homologi tertinggi berkisar 99% dengan Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) asal Indonesia, Malaysia, dan Thailand. Hasil deteksi dan identifikasi ini merupakan laporan pertama infeksi TYLCKaV dan asosiasinya dengan betasatelit Begomovirus pada terung di Bengkulu.
Virus Identification in Yard Long Bean Plants with Yellow Mosaic Symptoms Sutrawati, Mimi; Aulia, Ewa
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 2 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.2.116-121

Abstract

Begomovirus infection, which causes yellow leaf curl disease, is commonly found in the Bengkulu province, affecting various plants, including chilli, melon, cucumber, papaya, and weeds. The viruses reported to date in long bean plants include Mungbean yellow mosaic India virus (MYMIV) and Bean common mosaic virus (BCMV). This study aimed to detect viruses in long bean samples collected from Musi Rawas (South Sumatra) and North Bengkulu (Bengkulu), areas where yellow mosaic symptoms resembling those caused by Begomovirus infection were observed. Virus detection was performed using PCR-based DNA analysis with specific MYMIV (MY1/MY2) primers, targeting a DNA fragment of approximately 238 bp. The results revealed that yard-long bean plants from Musi Rawas were positively infected with MYMIV, while those from North Bengkulu tested negative for MYMIV. Begomovirus or other viral infections may cause the yellow mosaic symptoms observed in North Bengkulu yard long beans. Therefore, further investigations using additional primers are needed to identify the specific virus responsible for the symptoms in this region.
Tingkat Pengetahuan Dan Keterampilan Petani Tentang Tanaman Refugia Dan Musuh Alami Hama Padi di Desa Sumber Agung, Bengkulu Utara Hamidi, Ilmi; Djamilah; Sutrawati, Mimi; Zarkani, Agustin; Sitohang, Enjelina Br
DHARMA RAFLESIA Vol 22 No 2 (2024): DESEMBER (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v22i2.38363

Abstract

Penggunaan pestisida kimia yang berlebihan dalam pengendalian hama padi di Desa Sumber Agung, Bengkulu Utara, telah menimbulkan dampak negatif terhadap lingkungan dan kesehatan. Oleh karena itu, diperlukan pendekatan ramah lingkungan, seperti pemanfaatan tanaman refugia dan musuh alami, untuk mendukung keberlanjutan pertanian padi. Program pengabdian masyarakat ini bertujuan meningkatkan pengetahuan dan keterampilan petani tentang manfaat tanaman refugia dan peran musuh alami dalam pengendalian hama. Metode kegiatan meliputi observasi, pelaksanaan pelatihan interaktif, dan evaluasi menggunakan pre-test dan post-test. Hasil menunjukkan peningkatan skor rata-rata pengetahuan petani tentang tanaman refugia dari 1,50 menjadi 3,00, peran musuh alami dari 1,70 menjadi 3,60, dan keterampilan diagnostik hama padi dari 1,70 menjadi 3,20. Pelatihan ini berhasil meningkatkan pemahaman petani terkait teknik pengelolaan hama berbasis ekologi yang mengurangi ketergantungan pada pestisida kimia. Edukasi lebih lanjut diharapkan dapat memperluas adopsi strategi pengendalian hayati untuk mendukung produktivitas dan kelestarian ekosistem pertanian di wilayah ini.
Assessment on Genetic Diversity and Relationship of 19 Bird Pepper Genotypes Based on Morphological and SSR Markers Rustikawati, Rustikawati; Herison, Catur; Sutrawati, Mimi; Prameswari, Wuri
AGRIVITA Journal of Agricultural Science Vol 47, No 1 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i1.4447

Abstract

A comprehensive assessment of genetic diversity offers invaluable insights to breeders. This study aims to analyze the genetic diversity of 19 cayenne pepper genotypes using morphological and SSR markers.  Morphological markers are essential for evaluating visible traits while SSR markers provide deeper insights into genetic variation. Using both morphological and SSR markers provides a more robust, multidimensional approach to understanding genetic diversity. Morphological characterization was conducted on 19 bird pepper genotypes using 30 distinct traits, further divided into 95 sub-traits, to evaluate vegetative and generative growths. Molecular profiling was carried out using 10 SSR primers. The SSR analysis yielded 80 scorable bands, of which 72 were polymorphic. The high polymorphic information content suggests that these markers were effective in differentiating the genotypes.   The genetic diversity within the population was substantially high, providing valuable genetic material for breeding programs. Genetic similarity analysis divided the population into six distinct groups. Among them, Group I (genotype A05 Taiwan) and Group VI (genotype A02, Bengkulu Indonesia) formed unique, solitary groups, distinguishing them from other clusters. This information is highly beneficial for bird pepper cultivar development, emphasizing the importance of incorporating additional traits to align with the cultivar development of high yielding and saline-stress tolerance.
Growth and Yield of Lettuce (Lactuca sativa L.) by Giving Cow Manure and Empty Palm Oil Bunch Fertilizer on Peat Soil Manullang, Yedija; Herawati, Reny; Handajaningsih, Merakati; Ganefianti, Dwi Wahyuni; Haquarsum, Eka J.V.; Sutrawati, Mimi
Akta Agrosia Vol 23 No 2 (2020)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lettuce (Lactuca sativa L.) is a horticultural plant that is beneficial for health. Efforts are being made to expand the plant area. One of them is by utilizing marginal land such as peatlands.  Peatlands in Indonesia are the most extensive soils in various tropical countries in the world that have low soil fertility and high acidity so that they are not optimal for lettuce growth. The provision of organic material can increase soil fertility by improving physical, chemical, and biological soil properties so that it can support lettuce growth. This study aims to determine the dose of cow manure and the dosage of oil palm empty fruit bunch fertilizer and the optimal dose interaction for growth and yield of lettuce. This research was conducted from June 2019 to August 2019 in Bengkulu City. This study uses a Completely Randomized Design consisting of two factors, which are repeated three times. The first factor is the dose of cow manure with three levels, namely: 0 tons ha-1, 15 tons ha-1, and 30 tons ha-1. The second factor is the dose of oil palm empty fruit bunch fertilizer, which consists of four levels, namely 0 tons ha-1, 5 tons ha-1, 10 tons ha-1, and 15 tons ha-1. The results showed that the dosage of cow manure 15 tons ha-1 and without the application of empty fruit bunches fertilizer gave the best results in leaf length of 18.674 cm. The best dose of cow manure for growth and yield of lettuce is 15 ha-1. The application of OPEFB fertilizer has not been shown to affect the growth and yield of lettuce.
Genetic Diversity of Papaya Using Molecular Markers Random Amplified Polymorphic DNA Aulia, Ewa; Sutrawati, Mimi; Simarmata, Marulak
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.1.17-22

Abstract

Papaya is one type of fruit that is widely consumed and cultivated by the farmer. However, genetic analysis has not been carried out on various types of papaya available on the market. This aims to determine the genetic diversity of papaya plants that can become genetic resources to fullfil food needs and genetic resources for breeders. Genetic analysis was conducted by Random Amplified Polymorphic DNA (RAPD) method using 11 papaya varieties consisting of Calina, Bangkok, Red Pomegranate, Sunrise, Orange Lady, Red Lady, Taiwan, Arum, Miba, Golden, and Local and using 15 RAPD primers consisting of OPA-1, OPA-2, OPA-8, OPA-16, OPC-4, OPC-11, OPC-13, OPC-20, OPD-20, OPE-2, OPE-6, OPE-11, OPE-14, OPM-6, and OPY-15. PCR-RAPD results were translated into binary data and then cluster analysis was conducted using the Unweighted Pair-Group Method Arithmetic (UPGMA) method using the Numerical Taxonomy and Multivariate System (NTSYS) program. The PCR-RAPD results of 11 papaya varieties successfully amplified 8 out of 15 primers that formed 112 DNA bands with 85 polymorphic bands. Genetic diversity analysis showed the results at 90% similarity coefficient formed 9 groups. Group 1 consists of Bangkok and Sunrise varieties. Group 2 consists of Red Pomegranate and Arum varieties. Groups 3 to 9 consisted of Calina, Miba, Local, Golden, Orange Lady, Red Lady, and Taiwan varieties, respectively.