Article Per Year (5 Year)
p-Index From 2021 - 2026
3.512
P-Index
This Author published in this journals
All Journal Jurnal Fitopatologi Indonesia Agrikultura Jurnal Ilmu-Ilmu Pertanian Indonesia AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGRITROPICA : Journal of Agricultural Sciences JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Martabe : Jurnal Pengabdian Kepada Masyarakat Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Akta Agrosia Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Jurnal PADAMU NEGERI (Pengabdian pada Masyarakat Bidang Eksakta) Jurnal AGRISEP: Kajian Masalah Sosial Ekonomi Pertanian dan Agribisnis
Mimi Sutrawati
Universitas Bengkulu
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Transportation Other

Published : 29 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 29 Documents
Search

 1   2   3 
PENYAKIT TUNGRO PADA TANAMAN PADI (Oryza sativa) DI KECAMATAN TABA PENANJUNG : INSIDENSI PENYAKIT DAN DETEKSI VIRUS SECARA MOLEKULER Abdul Fiddin; Mimi Sutrawati; Hendri Bustamam; Dwi Wahyuni Ganefianti; Sipriyadi Sipriyadi
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 23 No 1 (2021)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.23.1.37-45

Abstract

[TUNGRO DISEASE ON RICE PLANT (Oryza sativa) IN TABA PENANJUNG DISTRICT: DISEASE INCIDENCE AND MOLECULAR DETECTION OF VIRUSES]. Tungro is one of the important diseases of rice plants. Tungro disease caused by Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). This research aims to obtain information on the spread of the disease, vector population, and molecular detection by polymerase chain reaction method. This research was conducted from December 2019 to April 2020 in rice fields in Taba Penanjung sub-district, Bengkulu Tengah district at ± 540 meters above sea level and virus detection in the Laboratory of Biotechnology and Genetics Department of Biology Faculty of Mathematics and Natural Sciences (MIPA) and Laboratory of Plant Protection, Agriculture Faculty, Bengkulu University. Data analysis used descriptive analysis. The results showed that the highest disease incidence on the seven weeks after planting (wap) is 1.7% and the highest population of the vector on the 7 wap with an average of 7.7 vector/100m2 in the village of Taba Penanjung. Detection of viruses with the PCR method with the specific primer DAF/DAR succeeded in amplification of DNA band measuring 1,400 bp. RTSV was not detected by RT- PCR using a specific primer or RTSV. The result of this detection shows that the symptoms of tungro on rice plants in the cultivation of Taba Penanjung district associated with RTBV infection. RTSV unable to detect can be caused by many factors in the detection process, including total RNA extraction, cDNA synthesis, DNA amplification, and DNA visualization of PCR products. 
DETEKSI MOLEKULER DAN ANALISIS GENETIK BEGOMOVIRUS PADA TANAMAN CABAI DI DESA PEMATANG DONOK Ewa Aulia; Mimi Sutrawati; Tunjung Pamekas
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 24 No 2 (2022)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.24.2.69-74

Abstract

[MOLECULAR DETECTION AND GENETIC ANALYSIS OF BEGOMOVIRUS ON CHILLI IN PEMATANG DONOK VILLAGE]. Many symptoms of yellow curly leaf disease, but there have been no reports of viral species infected the chili plants.   The research was aimed  to determine the disease incidence of yellow leafcurl disease and detection of Begomovirus infected chilli plants in Kepahiang regency based on  polymerase chain reaction (PCR). Sampling was done by purposive sampling method based on the symptoms; yellow leafcurl, leaf malformation, and stunting. Virus detection with PCR method using general primer Begomovirus (SPG 1/2). The PCR product was sent to First Base, Malaysia for DNA sequencing. Sequencing data were analyzed using MEGA 6 (Molecular Evolutionary Genetics Analysis) software for phylogenetic tree construction. Based on field observations, the incidence of disease ranged from 96.4%-100% and the whitefly vector insect (Bemisia tabaci) was found. PCR using general primers Begomovirus obtained DNA bands measuring 912 bp according to the primary target. Based on the Blastn results, the nucleotide sequences of three Begomovirus samples from Pematang Donok Village had nucleotide similarities with the Pepper yellow leaf curl Indonesia virus (PYLCIV) isolates from Bali and Java contained in the GenBank database, with a nucleotide similarity value of 91.77%-98.99%.  
CONSUMER PREFERENCES ON NEW CHILLI VARIETIES OF BENGKULU UNIVERSITY Arianti, Nyayu Neti; Ganefianti, Dwi Wahyuni; Sutrawati, Mimi
Jurnal AGRISEP JURNAL AGRISEP VOL 18 NO 02 2019 (SEPTEMBER)
Publisher : Badan Penerbitan Fakultas Pertanian, Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.655 KB) | DOI: 10.31186/jagrisep.18.2.331-342

Abstract

This research was conducted to 1) Analyze the perception of consumers on the attributes of chili, and 2) Know which chili products are selected by consumers based on shape,  size and which are most wanted to buy. Forty consumer respondents were taken by accidental sampling technique.  Consumers are asked to assess 10 types of the UNIB chili varieties, both monoculture and combination polyculture between varieties. The results showed that the majority of respondents stated that the size and shape attributes were important as the basis for the decision to buy chili, while the attributes of uniform size and shape are considered normal.  The variety of chili which consumers like because of their size, shape and desire to be purchased were UNIB C H13. While for this type of combination, the combination of UNIB C H13 and UNIB C H53 is desired to be purchased by consumers. This type of combination should be cultivated by farmers because it is resistant to pests, high productivity and preferred by consumers.
PENGEMASAN VAKUM PADA GURAMI PRESTO UNTUK MENINGKATKAN KUALITAS PRODUK DI KOPERASI PRODUKSI AMANAH SEJATI KOTA BENGKULU Anis, Ulfah; Sutrawati, Mimi
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 5, No 6 (2022): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v5i6.2303-2308

Abstract

Gourami fish has been cultivated and marketed by the Amanah Sejati cooperative located in Bengkulu city. Gurami is sold as fresh product. The price is not comparable with the cost of gurami cultivation. Therefore, it is necessary to make an effort to increase the economic value of the gourami fish. One of the solutions is to make it into presto gourami products. The gourami fish is then vacuum-packed to improve the quality and extend its shelf life. Community service aims to (1) provide knowledge about the type of packages, and (2) provide knowledge about how to vacuum packaging on presto gourami. These community services are coordination and socialization, counseling, and practice. These services were going well. The participants were enthusiastic about the counseling and practice.  
Detection and Sequence Analysis of Pepper Yellow Leaf Curl Virus Isolates That Infected Chili (Capsicum annuum L.) in Bengkulu, Indonesia Gustian, Deri; Sutrawati, Mimi; Fauzi, Ariffatchur; Hamidi, Ilmi; Mengesha, Nakachew Minuye; Do, Duy-Hung; RATTANA, Nitchayamon
AGRITROPICA : Journal of Agricultural Sciences Vol. 8 No. 2 (2025)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.8.2.74-80

Abstract

Pepper yellow leaf curl Aceh virus (PepYLCAV) and pepper yellow leaf curl Indonesia virus (PepYLCIV) are begomoviruses that dominate chili cultivation in Indonesia. Characterization of these two begomoviruses is essential as basic information for the development of virus control technologies. The symptoms on chili plants indicate that PepYLCIV is more severe than PepYLCAV, with severe mosaic, curling, and yellowing, whereas PepYLCAV causes only mild mosaic and yellowing. Sequencing results also show that the nucleotides and amino acids between PepYLCAV and PepYLCIV are significantly different. This suggests that the pathogenicity of the two viruses on chili plants differs. Protein structure predictions using AlphaFold3 also show significant differences. Based on available data, control of PepYLCAV and PepYLCIV can be achieved using different approaches, as these viruses have distinct sequences that may affect their pathogenicity.
Penerapan Teknologi Pengendalian Terpadu untuk Penyakit Tanaman Hortikultura di Desa Tapak Gedung, Kabupaten Kepahiang, Bengkulu Bustamam, Hendri; Sariasih, Yenny; Sutrawati, Mimi; Handayani, Dewi; Alimansyah, Alimansyah; Adelia Putri Khaila; Apri Fajar Susmiati
Jurnal PADAMU NEGERI (Pengabdian pada Masyarakat Bidang Eksakta) Vol. 6 No. 2 (2025)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37638/padamunegeri.v6i2.1959

Abstract

Permasalahan utama budidaya hortikultura di Desa Tapak Gedung, Kabupaten Kepahiang, Bengkulu adalah tingginya ketergantungan petani terhadap pupuk dan pestisida sintetis serta rendahnya kemampuan petani dalam mendiagnosis penyakit tanaman secara tepat. Kondisi tersebut berdampak pada tingginya biaya produksi, risiko kesehatan, serta potensi kerusakan lingkungan. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan dan keterampilan petani dalam diagnosis awal penyakit tanaman hortikultura serta penerapan teknologi pengendalian hama dan penyakit terpadu berbasis agens hayati. Metode pelaksanaan meliputi penyuluhan, pelatihan, pendampingan, dan pembuatan demplot budidaya bawang merah dengan aplikasi agens antagonis Trichoderma sp. dan Streptomyces sp. Evaluasi dilakukan melalui pre-test dan post-test, observasi partisipatif, serta keberhasilan demplot. Hasil kegiatan menunjukkan peningkatan pemahaman petani mengenai perbedaan penyakit biotik dan abiotik, kemampuan petani dalam memperbanyak dan mengaplikasikan agens hayati, serta keberhasilan penerapan pengendalian hama dan penyakit terpadu pada demplot bawang merah dengan hasil panen yang sehat dan baik. Kegiatan ini berkontribusi pada penerapan pertanian hortikultura berkelanjutan yang ramah lingkungan dan berbasis hasil riset perguruan tinggi.
Pengendalian Hama dan Penyakit Terpadu pada Tanaman Bawang Merah di Desa Sumber Sari Kabupaten Kepahiang, Provinsi Bengkulu Hikmah, Hikmatul Husna Al Mursyidi; Sutrawati, Mimi; Bustamam, Hendri; Ginting, Sempurna; Khaila, Adelia Putri; Susmiati, Apri Fajar
DHARMA RAFLESIA Vol 23 No 2 (2025): DESEMBER (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v23i2.44046

Abstract

Petani hortikultura di Desa Sumber Sari, Kabupaten Kepahiang, masih bergantung pada pestisida sintetis untuk mengendalikan hama dan penyakit tanaman. Penggunaan pestisida sintetis secara terus menerus dapat menyebabkan resistensi organisme pengganggu tanaman, mencemari lingkungan, serta menimbulkan gangguan kesehatan bagi petani maupun konsumen. Kegiatan ini bertujuan untuk meningkatkan pengetahuan petani dalam mengenali gejala penyakit tanaman serta keterampilan petani dalam memproduksi dan mengaplikasikan biopestisida secara mandiri dengan menggunakan agens hayati Trichoderma sp. dan Streptomyces sp.. Metode kegiatan meliputi sosialisasi, penyuluhan, pelatihan teknis, praktik lapangan, serta pendampingan dan evaluasi melalui pre-test dan post-test. Pelatihan ini berhasil meningkatkan pengetahuan dan kapasitas teknis petani secara signifikan mengenai pengendalian hayati, dengan seluruh peserta (100%) menyatakan ketertarikan untuk menerapkan teknologi ini. Sebanyak 80% peserta memahami proses pembuatan biopestisida, dan 60% mampu mengaplikasikannya di lahan. Kegiatan ini menunjukkan bahwa pendekatan partisipatif yang menggabungkan teori dan praktik lapangan efektif dalam membangun kesadaran serta kemandirian petani menuju pertanian berkelanjutan. Perlu penguatan kelembagaan lokal dan pelatihan lanjutan serta fasilitasi akses terhadap bahan dan informasi guna memperluas adopsi teknologi pengendalian hayati di tingkat petani.
Respon Ketahanan Sebelas Varietas Pepaya terhadap Isolat Papaya ringspot virus Asal Rejang Lebong, Bengkulu Aulia, Ewa; Sutrawati, Mimi; Simarmata, Marulak
Jurnal Agrikultura Vol 37, No 1 (2026): April, 2026
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v37i1.68714

Abstract

Gejala mosaik kuning ditemukan pada berbagai jenis tanaman pepaya di Rejang Lebong, Bengkulu namun, gejala terbanyak ditemukan pada pepaya varietas Calina. Gejala mosaik kuning pada tanaman pepaya dapat disebabkan oleh infeksi Papaya ringspot virus (PRSV). Untuk mengurangi  penyebaran penyakit di lapangan perlu dilakukan upaya pengendalian berupa penggunaan varietas tahan. Untuk mendapatkan  varietas tahan, perlu dilakukan pengujian respon ketahanan tanaman. Dalam penelitian ini, respon ketahanan 11 varietas pepaya yang telah tersedia di pasaran diuji terhadap isolat PRSV asal Rejang Lebong dengan tujuan untuk mendapatkan tanaman pepaya yang tahan terhadap infeksi PRSV asal Rejang Lebong. Dengan demikan,  dapat direkomendasikan kepada petani sebagai upaya pengendalian penyakit di lapangan. Varietas pepaya yang diuji adalah varietas Calina, Bangkok, Merah Delima, Sunrise, Orange Lady, Red Lady, Taiwan, Arum, Miba, Golden, dan Lokal yang diulang 5 kali dengan 3 tanaman per ulangan dan 3 tanaman tanpa inokulasi PRSV sebagai kontrol negatif. Inokulasi dilakukan secara mekanis menggunakan cairan perasan tanaman yang terkonfirmasi positif PRSV berdasarkan hasil deteksi virus secara serologi dengan metode Dot Immunobinding Assay (DIBA). Berdasarkan hasil matriks penilaian untuk masa inkubasi, insidensi penyakit, dan keparahan penyakit pada 11 varietas pepaya, ketahanan tanaman pepaya dibagi menjadi 3 kategori: tahan, moderat, dan rentan. Tanaman tahan terdiri dari pepaya varietas Orange Lady, Red Lady, Taiwan, dan Miba. Tanaman moderat terdiri dari pepaya varietas Sunrise, Arum, Golden, dan Lokal. Tanaman rentan terdiri dari pepaya varietas Calina, Bangkok, dan Merah Delima. Oleh karena itu, penggunaan varietas tahan menjadi solusi untuk pengendalian PRSV pada tanaman papaya di Kabupaten Rejang Lebong.
First Detection of Major Viruses Causing Mixed Infections in Melon (Cucumis melo L.) in Bengkulu Province, Indonesia Mimi Sutrawati; Hendri Bustamam; Sempurna Br. Ginting; Ratna Nabila Pradita; Ella Mustika; Ewa Aulia
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 2 (2025): December 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.2.108 - 118.2025

Abstract

Melon cultivation has increased rapidly in recent years and is frequently associated with widespread virus-like symptoms in Bengkulu Province, Indonesia; however, thereis no formal documentation of the mixed viral infections has been available. Therefore, this study aimed to detect major viruses infecting melon crops in Bengkulu Province and to evaluate their occurrence based on field symptoms and serological detection. Field surveys were conducted at three melon cultivation sites: Kandang Limun Village (Bengkulu City), Air Sebakul Village (Central Bengkulu Regency), and Tawang Rejo Village (Seluma Regency). Symptomatic leaf samples were collected purposively. Virus detection was performed using the Dot Immunobinding Assay (DIBA) with antisera specific to Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV), and Papaya ringspot virus (PRSV). The results showed that the dominant symptoms of viral infection in melon plants included mosaic, vein banding, chlorosis, leaf curling, and stunted growth in both Snow & Rock and Merlin F1 varieties. Disease incidence varied among varieties and locations, with the highest incidence recorded in Snow & Rock (73.3%). Serological detection revealed that 100% of symptomatic samples tested positive for CMV, TMV, and PRSV, confirming that all infections occurred as mixed infections. This study represents the first report of mixed viral infections in melon crops in Bengkulu Province. These findings demonstrate that overlapping and variable field symptoms cannot be attributed to a single virus and cannot be reliably distinguished through visual diagnosis alone, highlighting the importance of laboratory-based diagnostic approaches for accurate virus identification.
Co-Authors Abdul Fiddin Adelia Putri Khaila Agustin Zarkani Alamria, Intan Suryani ALI NURMANSYAH Alimansyah, Alimansyah Andreani Kinata Anwar, Guswarni Apri Fajar Susmiati Atra Romeida Aulia, Ewa Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Dewi Handayani Djamilah Djamilah Djamilah Djamilah Djamilah, Djamilah Do, Duy-Hung Dwi Wahyuni Ganefianti Dwi Wahyuni Ganefianti Dwi Wahyuni Ganefianti, Dwi Wahyuni Eko Supriyono Ella Mustika Ewa Aulia Ewa Aulia Fausiah T. Ladja Fauzi, Ariffatchur Gede Suastika Gede Suastika Gustian, Deri Hamidi, Ilmi Haquarsum, Eka J.V. Hendri Bustamam Hendri Bustamam Hendri Bustamam Herison, Catur Hikmah, Hikmatul Husna Al Mursyidi Khaila, Adelia Putri Listihani, Listihani Manullang, Yedija Marlin Marlin Marpaung, Nia Kurniati Br. Marulak Simarmata Mengesha, Nakachew Minuye Merakati Handajaningsih Nabillah, Ade Nadrawati, Nadrawati Novanda, Ridha Rizki Nyayu Neti Arianti Nyayu Neti Arianti Nyayu Neti Arianti, Nyayu Neti Parwito Parwito, Parwito Priyatiningsih Priyatiningsih Priyatiningsih Ratna Nabila Pradita RATTANA, Nitchayamon Reny Herawati Rustikawati Sari, Icha Kurnia Nanda Selangga , Dewa Gede Wiryangga Sempurna Br. Ginting SEMPURNA GINTING SEMPURNA GINTING Sipriyadi Sitohang, Enjelina Br Sobir Sobir SRI HENDRASTUTI HIDAYAT Susmiati, Apri Fajar Tunjung Pamekas Tunjung Pamekas, Tunjung Ulfah Anis Witanto Madyo Utomo Wuri Prameswari Yenny Sariasih Yenny Sariasih