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Journal : Journal of Fisheries

Characteristics of Water-Soluble Collagen Extracted from Catfish (Pangasius sp.) Skin Using Different Acetic Acid (CH3COOH) Concentrations S.Pi., M. Si, Patmawati; Nuzil Romadhoni, Puput; Puspitaningsih , Devi; Sulmartiwi, Laksmi; Nirmala, Dwitha; Endi Rahmantyo, Lastiko; Moechthar, Oemar; Raseetha, Siva; Akmal Alwi Husein, Mohamad; Khadijah Zai
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.72086

Abstract

Graphical Abstract Highlight Research Optimized extraction enhances fish skin-based collagen as an alternative to bovine and porcine sources. Hydro-Extraction Advantage offer a cost-effective approach for producing high-purity, water-soluble collagen. Varying CH₃COOH concentrations influence collagen yield and solubility, impacting its potential applications in food, pharmaceutical, and cosmetic industries. Hydro-extracted collagen is biodegradable and free of harmful residues.     Abstract Collagen extraction from fish skin offers a sustainable approach to valorize fish processing by-products, and fish skin catfish (Pangasius sp.) is recognized as a promising collagen source. However, previous studies reported that in low water solubility, limiting its functional application in food, pharmaceutical, and cosmetic industries. To address this challenge, this study investigates the use of varying acetic acid concentrations (0.4, 0.6, and 0.8 M) during the hydrolysis stage prior to hydro-extraction, aiming to enhance the solubility and quality of the extracted collagen. Parameters measured included yield, solubility, proximate composition (protein, fat, air content, ash), organoleptic quality, molecular weight, amino acid profile and functional groups analysis. The best results were achieved with 0.6 M acetic acid, resulting in type I collagen coupled by the amide groups A, B, I, II, III, and molecular weights (65, 95, 130 and 270 kDa). The dominant amino acids identified was glycine. This treatment yielded a collagen extraction rate of 9.04% and solubility of 79.71%. The proximate composition included 67.34% protein, 14.87% fat, 8.48% moisture, and 10.69% ash. Organoleptic scores for appearance, odour, and texture were 7.80, 7.93, and 6.80, respectively. The collagen met the SNI 8076:2020 standard for protein content, moisture, and organoleptic attributes; however, fat and ash contents exceeded the specified limits. In conclusion, the acetic acid concentration significantly affects the physicochemical and sensory properties of collagen. Catfish skin shows strong potential as a raw material for collagen production, which supports its use in the food, pharmaceutical, and cosmetic sectors.
Characteristics of Different Chitosan Types on κ-Carrageenan Polyelectrolyte Complex (PEC) Bioplastics as Food Packaging Puteri, Dhena Celia Haryadi; Alamsjah, Mochammad Amin; Sulmartiwi, Laksmi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.74222

Abstract

Graphical Abstract   Highlights Research 1. This study investigates the impact of incorporating chitosan from different sources (shrimp, crab, and fish scales) with varying molecular weights on the mechanical characteristics of bioplastics used for food packaging. 2. The present study aims to optimize the volume ratio of κ-carrageenan and chitosan to form stable polyelectrolyte complexes (PECs) for bioplastic production. 3. This study evaluates the quality of tilapia fillets wrapped with κ-carrageenan and chitosan PEC bioplastics after 24 h storage. 4. The present study investigates the reaction mechanism involved in the formation of polyelectrolyte complexes between κ-carrageenan and chitosan for bioplastic production.     Abstract Bioplastic represents an eco-friendly alternative to synthetic plastic and can be derived from natural polysaccharides like carrageenan. κ-carrageenan is noted for its gel-forming properties, making it a common raw material for bioplastics. Mechanical properties values of κ-carrageenan bioplastics are usually below standard. The addition of materials such as chitosan can enhance those properties. Chitosan-carrageenan can form polyelectrolyte complexe (PEC) through electrostatic interactions without toxic crosslinking agents. Polymer’s molecular weight is a crucial factor influencing PEC formation. Chitosan's molecular weight varies based on the raw material and extraction process. This study aims to identify the most suitable type of chitosan for food packaging bioplastics using polyelectrolyte complex (PEC) method. Three types of commercial chitosan with different molecular weights were evaluated (shrimp, crab, and fish scale chitosan). Japanese Industrial Standards (JIS) were used for characterization assessment of bioplastics such as thickness, tensile strength, water resistance, water vapor transmission, and biodegradation rate as well as additional tests including Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) analysis, and Total Plate Count (TPC) on fish fillets. The findings indicated that crab chitosan-κ carrageenan PEC bioplastic exhibited optimal results with a thickness of 0.178 mm, tensile strength of 18.053 MPa, elongation at break at 211.73%, water resistance of 63.94%, water vapor transmission (WVT) of 0,001456 g/m2/day, biodegradation rate of 3.358% over 7 days, and the lowest TPC in fish fillets after 24 h, increasing from 4.39 log CFU/g to 7.45 log CFU/g. Molecular weight of chitosan was shown to significantly influence the PEC bioplastics’ characteristics.
Effect of Ultrasonic Assisted Extraction with Ethanol for Removing Lipid on Catfish (Pangasius sp.) Skin as a Collagen Source and Its Characteristics Agustina, Maulida; Patmawati, Patmawati; Mubarok, Shofy; Sulmartiwi, Laksmi; Wulandari, Diah Anggraini; Zai, Khadijah; Siva, Raseetha; Pujiastuti, Dwi Yuli; Nirmala, Dwitha; Carattri Kusuma Werdani, Money; Moechthar, Oemar; Alwi Husein, Mohamad Akmal
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.46061

Abstract

Abstract Fish skin is rich in collagen (80%-90%). However, catfish (Pangasius sp.) skin has a high lipid content, which can reduce the quality of collagen. Therefore, treatment is needed to remove the lipid using ultrasonic assisted extraction (UAE) with ethanol. Hence, the aim of the present study was to remove lipid content from catfish skin as a raw material for collagen by using UAE with different concentrations of ethanol (25, 50, and 75%). The research was conducted in three stages: removing impurities, collagen extraction, and characterization of collagen. The result exhibited that UAE-ethanol treatment was capable of removing up to 85.6% of lipid content, and the produced collagen had the potential to be utilized as an alternative source of collagen based on its properties. Highlight Research Ethanol could be used as extractor of lipid from catfish skin of Pangasius Ultrasonic assisted extraction could increase efficiency of lipid extraction from catfish skin. Fish skin of Pangasius sp. has a potential source for collagen. Collagen from skin of Pangasius sp. has a good characteristic with high protein content.
Co-Authors A. Shofy Mubarak Abdillah, Annur Ahadi Adriana Monica Sahidu Akmal Alwi Husein, Mohamad Alwi Husein, Mohamad Akmal Amalia, Nia Amalia, Raisya Amalia, Regal Amin, Muhamad Nur Ghoyatul Anggrahini, Witrishya Anika Candrasari Anisa Putri, Dede Apriliani, Anindya Aurora Aribah, Khansa Arifah, Nazhry Zahra Arum, Dyah Ayu Sekar Arum, Kartika Dwi Sekar Aulia, Mutia Arsya Bricareira Bara Presmasitha Carattri Kusuma Werdani, Money Carattri, Money Caroline, Patricia Cicik Kurniawati, Cicik Dewi Nur Intan Sari Dewi, Nina Nurmalia Dwi Yuli Pujiastuti Dwitha Nirmala Eka Nurinsani, Anisa Eka Saputra Elyanti, Ratih Nurfaizi Embiysne , Viamell Endi Rahmantyo, Lastiko Erna Herawati Erna Herawati Erna Herawati, Erna Euclida Qolbun Rahman Fachroni Rahman Fachroni Rahman, Fachroni Faizah , Ariyani Flora Ramona Sigit Prakoeswa Gunanti Mahasri Hamidah, Syalyva Hari Suprapto Heru Pramono Husein, Mohamad Akmal Alwi Janitra, Hadyan Karunia, Fitria Khadijah Zai Kusuma Ningrum, Sukma Lailatul Lutfiyah, Lailatul Larasati, Anastasya Dewi Lestari, Nining Maulida Agustina Maulidia, Cici Meilina, Sinta Cahya Melinda Kusuma Ningrum Mochammad Amin Alamsjah Mubarok, Shofy Muhammad Aji Akbar Muhammad Aji Akbar, Muhammad Aji Muhammad Shoim Dasuki N Nurhayani Nur Mahmudah Nur Mahmudah Nurhayani nurhayani Nuzil Romadhoni, Puput Oemar Moechthar Patmawati Patmawati, Patmawati Pradipta, Savalda Rosca Puspitaningsih , Devi Puteri, Dhena Celia Haryadi Putra, Bernathdo Mahendra Robbi Putri, Monica Angelina Rahma, Sitta Rahmawati, Ghishella Ayu Ramadhan, Novelix Arditan Raseetha, Siva Ratna Widyaningrum Retno Sintowati - Ridwansyah Rochmadina Suci Bestari Rohmatin, Aulia Rokhim, Akhmad Nur Rr. Juni Triastuti S.Pi., M. Si, Patmawati Sapto Andriyono Saputra, Alfian Rahmadhani Satria Hani Sari, Devi Elvina Sari, Dewi Nur Intan Shohifah, Isnatul Umu Sitta Rahma Siva, Raseetha Sri Wahyu B Sri Wahyu Basuki Titi Savitri Prihatiningsih Tri Agustina Tri Agustina, Tri Tri Nur Kristina Wahyu B, Sri Wahyuningtyas, Hibha Mahesti Wardhani, Citra Rachmania Widodo, Langgeng Woro Hastuti Setyantini Wulan, Novrida Wulandari, Diah Anggraini Yanti, Luh Krisna Weda Yudhistira, Ary Yuni Prastyo Kurniati zai, Khadijah Zhanifathul Abhidah, Noer Rahmah