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Journal : Jurnal Perlindungan Tanaman Indonesia

The Effectiveness of Several Plant Extracts to Induce Rice Plant Resistance against Bacterial Leaf Blight - (Xanthomonas oryzae pv. oryzae) Fitri Widiantini; Avissa Ayuningdiyas; Endah Yulia; Tarkus Suganda
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.34912

Abstract

Resistant plants are one of the disease control techniques that considered to be effective. Resistant plants can be produced in various ways including the application of plant extracts. The aim of this study was to examine the ability of several plant extracts to increase the resistance of rice plants to bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo). A total of 13 plants were extracted and applied in two methods, which were seed treatment and seedling treatment which sprayed on two-week old rice seedlings. Xoo bacteria were inoculated on rice plants two weeks after planting. The observations on the intensity of BLB disease infection showed that water hyacinth extract (Eichhornia crassippes), spiny amaranth (Amaranthus spinosus) and jasmine leaves (Jasminum grandiflorum) can suppress the development of BLB disease in both application methods. The application of plant extracts as inducing agents needs to be repeated to maintain the activated plant defense mechanism.
Antifungal Potency of Secondary Metabolites Produced by Endophytic Bacteria against Pathogenic Fungi Pyricularia oryzae Cav. Fitri Widiantini; Mia Rahmah Qadryani; Fuji Hartati; Endah Yulia
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.48392

Abstract

Blast disease caused by Pyricularia oryzae Cav. is one of the most important diseases on rice. One of the alternative controlling methods in P. oryzae is biological control through the utilization of secondary metabolites produced by endophytic bacteria. The study aimed to determine the antifungal potency of secondary metabolites produced by rice endophytic bacteria against P. oryzae. The experiment was conducted using 9 endophytic bacteria isolated rice (Os1, Os2, Os3, Os4, Os5, Os6, Os7, Os8, and Os10). Each isolates were grown in ISP2 liquid media and the secondary metabolites compounds were extracted using two different solvents; methanol and ethyl acetate : methanol (4:1) (v/v). The effect of secondary metabolites was tested using agar well diffusion method. The results demonstrated that the secondary metabolites extracted by both solvents have antifungal effect on the growth of P. oryzae. The highest growth inhibition was shown by secondary metabolites extracted by ethyl acetate : methanol (4:1) from Os1 (42%) and Os3 (39%). Antifungal activity of the secondary metabolites was indicated by the formation of clear zone. HPLC (High Performance Liquid Chromatography) analysis showed the differences of peaks and retention time between secondary metabolites produced by Os1 and Os3 which has antifungal activity and secondary metabolites produced by Os10 that did not show the antifungal activity.
Co-Authors Aditya Bintan Pratama Agung Karuniawan Agung Kurniawan Agus Susanto Agus Susanto Agus Susanto Aina Anna Roosda Andang Purnama Andang Purnama Andang Purnama Asri Peni Wulandari Avissa Ayuningdiyas Betty Natalie Fitriatin Ceppy Nasahi Citra Bakti, Citra Danar Dono Danar Dono Deni Ariyono Dewi, Vira Kusuma Dinda Sekar Ayu Fiko Dwindry Formanda Dwisari Januarily Pitaloka Elga Sari Ema Komalasari Ema Komalasari, Ema Emma Trinurani Sofyan Endah Yulia Endah Yulia Endah Yulia Endah Yulia Yulia Fajri Maulana Anugrah Fakhrurroja, Hanif Feliatra Fiko, Dinda Sekar Fitri, Elysa Fuji Hartati Fuji Hartati Ghifari, Raden Faqih Hilmiy Hadi Syahir Muhadam Hersanti - Hersanti HERSANTI Hilda Sandra Utami Ida Nurhelawati Ida Nurhelawati Ifa Dwi LUTHFIANA Kang, Seung Won Kartika Sari Kirana Sonya Harviana Krissanti, Petra Sulistya Dian Lindung Tri Puspasari Luciana Djaya, Luciana Margaretta Christita, Margaretta Mia Rahmah Qadryani Mitalo, Oscar W. Muhammad Maksum Nabilla, Anisa Nadhira, Nadhira Neneng S Widayani Nia Rossiana Nono Carsono Nono Carsono Noor Istifadah Nugraha, Gema Takbir Parakkasi, Karmilla Prabowo, Yudhisa Hendri Putra, Fitriansyah Putu Ghita Novilaressa Ramdan Firmansah Rangga Irawan Prasetyo RIKA MELIANSYAH Riswandi, Hafiz Rizqullah, Ahmad Fauzan Safri Ishmayana Santa Monica Santika Sari Satriyo Restu Adhi Septiandini, Adelia Serly Noviyawati Silviya Rahma Dhiya Syafira Siska Rasiska SIska Rasiska, SIska Sri Hartati Sri Hartati Sri Hartati Sudarjat Sudarjat Sukmono Suwignyo Susanti, Rista Syahnur, Fitika Syarif Hidayat Tarkus Suganda Tati Herlina Tiara Febricyanti Putri Senjaya Tualar Simarmata Vira Kusuma Dewi Vira Kusuma Dewi Wahyu Daradjat Natawigena, Wahyu Daradjat Wawan Kurniawan Yulia, Endah Yulia Yunira, Alma Yusup Hidayat Yusup Hidayat