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Journal : Journal of Fisheries

Fungsi Sel Imun Interleukin-4 (IL-4) pada Ikan Kerapu Tikus (Cromileptes altivelis) dengan Induksi Protein Imunogenik Vibrio harveyi [The Function Of Interleukin-4 (IL-4) Immune Cells On Humpback Grouper (Cromileptes altivelis) By Induction Of Immunogenic Protein Of Vibrio harveyi ] Uun Yanuhar; Asus Maizar SH; Ita Fitria S
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11588

Abstract

Abstract Humpback grouper (Cromileptes altivelis) is a commodity to be cultivated because it has a high price, but the grouper highly susceptible to Vibriosis (Vibrio harveyi). The research objective was to see the function of immune cells of interleukin-4 (IL-4) are formed due to the induction of immunogenic proteins of Vibrio harveyi. Research procedures include Vibrio harveyi immunogenic protein isolation, SDS-PAGE electrophoresis, elektroelusi and dialysis, clinical exam of Vibrio harveyi immunogenic proteins, and immunohistochemical to look at immune cells (cells IL-4) with using a secondary antibody labeling of anti IL-4 biotin conjugate. The results showed that Vibrio harveyi protein found have immunogenic properties is 51.16 kDa. This protein is an adhesin protein. Protein 51.16 kDa was purified by electroelution and the protein will be used as a vaccine administered by intraperitoneal injection in grouper (Cromileptes altivelis) at a dose of 31.6 ug protein / 150 g of fish weight. In organ tissues of the brain, eyes and kidneys of induced fish by immunohistochemical examination, It was detected Immune cells (cells IL-4) using a secondary antibody labeling of anti IL-4 biotin conjugate. Tues formed IL-4 can be seen on the network grouper indicated by a change in tissue color with an inverted microscope examination of the golden brown color is formed on the IL-4 immune cells. It show the cross-reaction between 51.16 kDa immunogenic protein induced in fish with anti IL-4 biotin conjugate. The conclusions is the protein of Vibrio harveyi with molecular weight of 51.16 kDa is an immunogenic protein which able to induce formation of IL-4 in grouper.
Pengaruh Pemberian Bahan Aktif Ekstrak Nannochloropsis oculata Terhadap Kadar Radikal Bebas Pada Ikan Kerapu Tikus (Cromileptes altivelis) yang Terinfeksi Bakteri Vibrio alginolyticus [The Influence Of Supply Of Bioactive Extract Nannochloropsis oculata To Dose Of Free Radicals In The Humpback Grouper (Cromileptes altivelis) That Was Infected By Vibrio alginolyticus] Uun Yanuhar
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 2 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i2.11687

Abstract

AbstractNanochloropsis oculata is one of kind green algae that overload in Indonesia ocean. The purpose of this research  was to know the potency antioxidant of Nannochloropsis oculata extract as in the Humback Grouper. The research was conducted by  experimently and used direct random sampling as design with 4 experiments and 3 repeats  i.e control fish, fish was  added Nannochloropsis oculata extract, Vibrio alginolyticus - infected fish, and Vibrio alginolyticus - infected fish that was added Nannochloropsis oculata extract. The result showed, fish that added by Nannchloropsis oculata extract made a different influence which was very clear to the decreasing of  free radical in the body. Free radical (malonaldehid) in the Vibrio alginolyticus -  infected fish was 4, 04 µg/100 mg which fish was added by Nannochloropsis oculata extract, the free radical level was decrease into 3, 29 µg/100 mg massa.
Mekanisme Infeksi Vibrio Pada Reseptor Ikan Kerapu Tikus Cromileptes altivelis [The Mechanism Of Vibrio Infection To Grouper Reseptor Cromileptes altivelis] Uun Yanuhar
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11693

Abstract

Abstract One of the most favourite fish that farmer fish cultivated because its high economic value was gouper (C. altivelis). The obstacle in cultivation system was caused by Vibrio algynolitycus. Vibrio angullarum, Vibrio parahaemolyticus. Vibrio harveyi infection. Many deseases that attacked to the grouper was caused by the role of pathogen specific as a ligan and its reseptor. The aim of these study was to improve and to know the role of reseptor in vibriosis mechanism with tested spesific reseptor protein of grouper Cromileptes altivelis. These study used experimental and descriptive methode with identified of grouper reseptor, Hemaglutinin test and observation use electron microscope. These study result showed that reseptor protein of C. altivelis was found in its eyes. These showed spesivity and reactivity to the vibriosis specific epitop.
Plastic Litter as Pollutant in the Aquatic Environment: A mini-review Rifka Rimbi Anggraini; Yenny Risjani; Uun Yanuhar
Jurnal Ilmiah Perikanan dan Kelautan Vol. 12 No. 1 (2020): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v12i1.17963

Abstract

HighlightThe issue of plastic litter and microplastics which is currently of special concern in global particularly IndonesiaThe review of size, colour and shape of microplastics that becomes part of the plastic litterThe observed review highlight of plastic litter and microplastics impact on environmental health particularly aquatic biotaAbstractThe negative impact that comes from plastic litter in the aquatic environment is a scourge for the entire world including Indonesia.Plastic litter has a huge influence on the most biota who lives in symbiosis with along the aquatic environment as it is able to sustainability. Moreover, there are other factors that cause the death of aquatic biota. Nowadays, the negative impact of plastic litter occurs on an ongoing basis through the food chain process which until now has not been well studied. Therefore, it is necessary to do further studies on the influence of the food chain as a vector of plastic litter distribution, particularly microplastic on aquatic biota from the first trophic level to high trophic levels. 
Effect of Aquaculture Wastewater And Zarrouk in Increasing Biomass, Protein, and Carotenoids levels of Spirulina platensis Anisa Millah Taqiyyah; Yenny Risjani; Asep Awaludin Prihanto; Uun Yanuhar; Mohammad Fadjar
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.40822

Abstract

Highlight Research S. platensis has been analyzed for its productivity. Analysis of the productivity of S. platensis on the use of fish culture wastewater media. S. platensis production can be increased by aquaculture wastewater. Aquaculture wastewater enhances the production of cell density, SGR, biomass, protein, and carotenoid of S. platensis. Abstract Increased productivity of Spirulina sp. in the form of high protein, carotenoids, and biomass content can be achieved by improving its nutrient supply. Inorganic fertilizers are nutrient sources, which are generally used in the culture of this organism on laboratory and industrial scale, but there are several drawbacks, including their high costs and limited availability. Several studies have also reported the use of zarrouk fertilizer as a standard culture medium for Spirulina platensis. Therefore, this study aims to determine the effect and the best concentration of fish culture wastewater treatment in Spirulina platensis culture using biomass, protein content, and carotenoid pigments as indicators. A two-factorial completely randomized designs (CRD) was used in this study, where the factors include the dose of organic waste and Zarrouk fertilizer. The microalgae samples, namely S. platensis were cultured using fresh water. This research consists of two factors. The first factor is the dose of organic waste, and the second factor is the dose of Zarrouk fertilizer. The wastewater treatment consisted of 0, 2, 4, and 6 ml/L, while Zarrouk dosages include 0, 0.5, and 1 ml/L. The best results were obtained from the sample treated with 6 ml/L aquaculture wastewater without the addition of Zarrouk. Furthermore, this treatment gave biomass production of 0.781 g/L, 50.441% protein, and 1.246 mg/L carotenoids. Based on the results, S. platensis culture can be carried out using fish culture wastewater without the addition of Zarrouk fertilizer.
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Alfarisi, Muhammad Asnin Alfi Nur Rusydi Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Baihani Amira Masitha Anasiru, Arief Farhan Kurniawan Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Annisa Fitri Anggreini Apri Supii Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Bili Darnanto Susilo BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Defa Rizqi Machfuda Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Ekwan Nofa Wiratno Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Fitrianesia, Fika Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gilang Rusrita Aida Gilang Rusrita Aida Gunanti Mahasri Hardoko Hardoko Hartati Kartikaningsih Herlina, Cucun Herly Evanuarini Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Kharisma Nuria Fitri Lutfi Ni`'Matus Salamah Lutfia Fahimatul Ilmi MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Maria Kristiani Marsono Marsono Martiningsih, Nur Fauzi-yah Martiningsih, Nur Fauziyah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Fadjar Mohammad Musa Mufidah, Rhobithotus Muhamad Muhajir Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nezya Pramudya Wardani Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika R Adharyan Islamy Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari, Septi Setyayunita, Tamaryska Shiddiq, Muhammad Faiq Ash Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Umi Zakiyah Utari, Inpita Casuarina Eqisetia Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Arif Wahyudi Yusuf Wahyudi Zainal Abidin