Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

2.125

P-Index

This Author published in this journals

All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Peternakan Tropika ARSHI Veterinary Letters Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences

Gusti Ayu Yuniati Kencana

Laboratorium Virologi Fakultas Kedokteran Hewan Universitas Udayana, Denpasar

Author-ID : 425703

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 55 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

SEROPREVALENSI PENYAKIT AVIAN INFLUENZA SUBTIPE H5N1 PADA AYAM BURAS DI PASAR BERINGKIT DAN GALIRAN, BALI Baiq Indah Pertiwi; Gusti Ayu Yuniati Kencana; I Nyoman Suartha
Jurnal Sain Veteriner Vol 38, No 3 (2020): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55896

Avian Influenza (AI) is a strategic communicable and zoonotic disease. The cause is a virus with Highly Pathogenic Avian Influenza (HPAI) subtype H5N1. The poultry market has important roles in the preservation, propagation, and spreads of the Avian Influenza (AI) virus from poultry to other species and humans. The purpose of this study was to determine the level of seroprevalence of Avian Influenza (H5N1) in free-range chickens at the Beringkit and Galiran market. A total of 120 free-range chickens were used as a sample. They have taken 60 serum samples per market. Serum removal is made from 5 merchants under the provisions of 3 samples from merchants who sell 6 to 10 free-range chickens. The sampling period is carried out for 2 months every 2 weeks 4 times. Sample testing was performed at the Denpasar Veterinary Centre with Haemagglutination (HA) and Haemagglutination Inhibition (HI) as barriers. The data titer of the antibodies obtained was analyzed by Non-Parametric Statistic Test Chi-Square (χ2) using IBM SPSS for windows. The results of the study showed that the AI subtype of the H5N1 subtype in both Beringkit Markets is 3.3% and Galiran Market is 6.7%, with seroprevalence in the two markets of 5.0% which is statistically not dissimilar (P<0.05). To prevent the transmissions of AI disease at the Beringkit Market and Galiran is recommended for vaccination and more attention to the market management and the free-range chicken maintenance system.

Respon Imun Seluler Ayam Petelur Pascavaksinasi Avian Influenza Subtipe H5N1 Isolat dari Bali Gusti Ayu Yuniati Kencana; Tri Komala Sari; I Nyoman Suartha; I Ketut Tomy Caesar Ramanda; Anak Agung Sagung Kendran
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.66086

Avian Influenza subtype H5N1 (AI-H5N1) is a malignant virus that is very detrimental to laying chickens because it is highly contagious and mutates easily. Prevention of AI-H5N1 disease in laying chickens is carried out by vaccination, therefore to maintain the quality of the vaccine, continuous research is needed. This study aims to determine the potential of AI-H5N1 vaccine isolates from Bali as measured based on cellular immune response based on total and differential leukocyte cells. Formation of antibodies is influenced by the nonspecific and specific immune system involving leukocytes, especially lymphocytes. Total of 40 layers of Novogen Brown strain were used for the research sample, kept since the age of one day on a commercial farm in Perean Village, Tabanan Regency, Bali. Laying chickens are vaccinated at 5 weeks of age by intramuscular injection. Total of 20 chikens were taken randomly and used for the sample. Blood draws were performed once pre-vaccination and five times each week after vaccination with anticoagulants. Total leukocytes were examined by an auto hematology analyzer, while differential leucocytes with thin blood smear stained with Giemsa. Total and differential leukocyte data were analyzed by means of the variance test followed by the Duncan test. Results showed that AI-H5N1 vaccination from Bali isolates could increase total and differential leucocytes of laying chickens and had significant effect on the mean total leukocytes, the absolute values of heterophyll cells, eosinophils, lymphocytes, and monocytes, but had no significant effect on post-vaccination basophil cells.

The Stability of the Bali’s Rabies Virus Molecular Marker for the Development of Diagnostic Method Putu Dimas Abiyoga; Gusti Ayu Yuniati Kencana; I Nyoman Dibia
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.457 KB)

The objectives of this study were to prove that the molecular marker of Bali’s rabies virus is still conserveand to develop a diagnostic method based on molecular marker. Thirty brain samples of dog that had beeninfected by a rabies virus from 2014 and 2015 were used for this research. The sequences of nucleotidewhich were obtained and the sequences of nucleotide accessed in GenBank were analyzed using MEGA5.2 software. The result provided that the specific amino acid (isoleusin) at position 308 (open readingframe) as a molecular marker of Bali’s rabies virus was still conserve. Fragment of N gene amplified byReverse RT-PCR method with a specifically designed primers showed that every isolate of rabies virus hadit’s own typical band, and could distinguish Bali isolate from the others in Indonesia.

Seroprevalensi Infeksi Virus Newcastle Disease dan Deteksi Paramyxovirus Pada Itik di Peternakan dan Pasar Unggas di Bali I GBA Purwanda; I Gusti Ngurah Kade Mahardika; Gusti Ayu Yuniati Kencana
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.823 KB)

Seroprevalence of Newcastle Disease Virus (NDV) infection and the presence of paramyxovirus on ducksat the farmland and the poultry market in Bali have not been known. The purpose of this study was todetermine the comparison of seroprevalence of NDV infection and the presence of paramyxovirus on duckat farmlands and poultry markets in Bali. Locations sampled were Gelgel, Tojan, Akah, Takmung, Tusanvillages in Klungkung Regency, as well as Mengwi, Mengwitani, Lukluk, Sangeh, and Blahkiuh villages ofBadung Regency. The poultry market samples were Galiran of Klungkung Regency, and Bringkit ofBadung Regency. Serum samples and cloacal-tracheal swabs were taken using stratified-random samplingfrom adult ducks of both markets and farmland that had more than 500 individuals in a flock. Samplingwas carried out every month for 6 months. Antibody against NDV was detected with InhibitionHaemagglutination test (HI). Tracheal and cloacal swabs were propagated in fertile chicken eggs of 9-11days old. Paramyxovirus was detected by the haemagglutination (HA) test and Reverse TranscriptasePolymeraseChain Reaction (RT-PCR). The correlation between NDV seroprevalences at farmland andpoultry markets was analyzed using non-parametric test of Chi-square. The results showed that theseroprevalence of NDV on March until August 2012 reached 45% on farmlands in both regencies, while inthe markets were up to 32.6%. There was no correlation between NDV seroprevalence at farmlands andpoultry markets in the two regencies (r = 0.522, P> 0.05). The paramyxoviruses detected were APMV-5and APMV-8, while NDV was not found.

Seroprevalensi Newcastle Disease pada Ayam Buras di Kecamatan Tabanan, Kabupaten Tabanan, Bali Ledi Natalia Surbakti; Gusti Ayu Yuniati Kencana; I Nyoman Suartha
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Newcastle Disease (ND) is one of the poultry diseases that can infect domestic chickens. It is an infectious disease that has a detrimental impact on chicken farmers. ND infection can be acute to chronic, and it can also affect all types of poultry, especially chickens, both broiler and domestic chickens. Tabanan Subdistrict is a location where poultry trade activities are often carried out between regions and/ or provinces, so domestic chickens have the potential to be exposed to ND. This study aims to determine the disease seroprevalence in domestic chickens in Tabanan District, Tabanan Regency, Bali. The sample of this study was serum from chicken’s blood samples that were taken from six villages in Tabanan Sub-district. Samples were taken from villages where each village consisted of three banjars. Banjar is a division of Bali administrative areas, and eight samples of domestic chickens were collected from each sampled banjar. A total sample of 144 serums of non-vaccinated domestic chicken. Serum was tested serologically with hemagglutination (HI) test at the Virology Laboratory of the Faculty of Veterinary Medicine, Udayana University. The results showed that ND seroprevalence in domestic chickens in Tabanan Subdistrict was 3.17%. Positive samples were gathered from Gubug, Bongan, and Wanasari villages with antibody titers ranged from 24 to 25. The conclusions of seropositive results showed that domestic chicken in the Tabanan district has currently been infected with the Newcastle Disease virus.

Peran Coding dan Non-Coding Region dari Gen Polimerase Kompleks Dalam Adaptasi Virus Avian Influenza Gusti Ayu Yuniati Kencana
Buletin Veteriner Udayana Vol. 4 No.1 Pebruari 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.289 KB)

Avian influenza (AI) is an avian diseases that can cause deadly humaninfection. The disease is caused by Orthomyxoviridae virus, genus influenzavirus type A,subtype H5N1. Pathogenicity of AI virus is polygenic, which means AI virus isdetermined by all the genes. Here the role of coding region (CR) and non-coding region(NCR) of polymerase gene complex is critically reviewed. The coding region of thepolymerase genes can be explained as follows. The amino acid position 627 PB2 gene isa factor adaptation of AI viruses in mammals. Polymerase basic-1 (PB1) protein acts as acentral activity of the enzyme catalyzing the viral polymerase. Polymerase Basic-1 bindsto the terminal end of the vRNA and the cRNA and shows endo-nuclease activity. Theactivity has been identified on the E508, E519, and D522. Polymerase Acid (PA) proteinsplay a role in supporting the biological activity of the polymerase gene complex, but itsmechanism of action in transcription, replication has not been disclosed as clear. Anotherrole of the PA protein is forming a complex of RNA polymerase and express anproteolytic role of cell proteins that suppress cell division. Polymerase acid gene has alsoserine protease activity has been identified at position S624. The non-coding regionmight also play role in the pathogenecity of influenza virus. The results of sequenceanalysis of non-coding region (NCR) at 5’-end of polymerase gene complex of AI virussubtype H5N1 from poultry and pigs in Indonesia showed that the NCR genes PB2, PB1and PA are homogeneous, whereas there are variants of the PB1 gene isolates from ducksA / Duck / Badung, 2006. Occurrence of deletions, insertions, and mutations in the NCRand CR polymerase complex genes may likely lead to genetic changes in viruses whichpotentially also change the nature of biology of AI virus. The study on the 3’-end of thegenes needs to be carried out.

Respon Imun Primer Ayam Petelur Pasca Vaksinasi Egg Drop Syndrome Gusti Ayu Yuniati Kencana; I Nyoman Suartha; I Putu Wira Adi Wibawa
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.936 KB)

This study was conducted to determine the primary immune response post vaccination using EDS inactivated vaccine polyvalent. The sample used was a commercial layer farm in the village of Tiga, regency of Bangli, Bali. A total of 25 layer which were14 weeks old vaccinated using EDS-76 inactivated vaccine containing polyvalent Newcastle disease antigen virus, infectious bronchitis and egg drop syndrome by intramuscularly injection. Examination of EDS antibody titer using serologic test by Hemagglutination Inhibition (HI) test. Egg drops syndrome antibody titer checked four times, once before vaccination, and every week for three weeks post-vaccination to see the immune responses. The average antibody titer then analyzed using an univariate of variance test followed by a test of Least Significant Difference, Duncan test and regression analysis. The result showed an increase antibody of EDS was significantly every week post vaccination. The average antibody titers of EDS are 22,6 HI unit at one weeks post vaccination, about 25,04 HI unit at two weeks post vaccination and 26,4 HI unit at three weeks post vaccination.

Ragam, Prevalensi dan Intensitas Infeksi Parasit pada Sapi Kelompok Tani Niti Sari Desa Baturiti Kabupaten Tabanan, Provinsi Bali Ida Ayu Pasti Apsari; Gusti Agung Ayu Yuniati Kencana; Gusti Ngurah Kade Mahardika; Nyoman Mantik Astawa; Anak Agung Sagung Kendran; I Nyoman Suartha; Srikayati Widyastuti; Ida Bagus Kade Suardana; I Gusti Ayu Mayani Kristina Dewi; I Putu Sudiarta
Buletin Veteriner Udayana Vol. 14 No. 1 February 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Parasitic infections are inseparable from the epidemiological triangle, namely host, agent and environment. Bali cattle as host of parasitic agents are affected by infection by environmental conditions in the village of Baturiti. The purpose of this study was to determine the prevalence and intensity of parasitic infections in cattle from the Niti sari farmer group in Baturiti village. A total of 55 Bali cattle in Niti Sari farmer group cattle were used as samples. Stool samples are taken to check for the presence of parasites and predict the amount of parasitic load that infects. The method of checking the presence of parasites by floatation test and prediction of parasitic load by the Stool method. The results obtained were the prevalence of Coccidia protozoa 52.73% (29/55), Entamoeba sp.16.36% (9/55), Balantidium sp.20.9% (6/55) with intensity of infection respectively 1255.17 ± 964.82 oocysts/gram, 233.3 ± 250 cysts /gram and 150 ± 83.67 cysts/gram feces. The prevalence of Strongyl worm eggs is 61.82% (34/55), Strongyloides sp. 23.64% (13/55) and Toxocara sp. 18.18% with an infection intensity respectively of 420.59 ± 233.26 eggs/gram, 253.85 ± 64.55 eggs/gram and 130 ± 48.3 eggs / gram. Conclusion of Bali cattle in the farmer group Niti Sari Baturiti village infected with the parasitic protozoa Coccidia, Entamoeba sp and Balantidium sp. with low to moderate intensity. Infection by Strongyl, Strongyloides sp. and Toxocara sp. nematodes with low intensity.

Penyebaran Virus Vaksin ND Pada Sekelompok Ayam Pedaging Yang Tidak Divaksinasi dan dipelihara bersama ayam yang divaksinasi Gusti Ayu Yuniati Kencana; Nyoman Mantik Astawa; I Gusti Ngurah Kade Mahardika; I Wayan Gorda
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.611 KB)

Telah dilakukan penelitian untuk mengetahui daya sebar vaksin ND aktif galurlentogenik (La Sota) dan respons immune ayam yang tidak divaksin yang dipeliharabersama ayam yang divaksin secara intramuskuler. Penelitian ini menggunakan rancanganacak lengkap pola berjenjang (split time) dengan faktor utama perlakukan vaksinasi (TO:0% divaksin dan 100% tidak divaksin , T1: divaksin 50 % dan 50 tidak divaksin dan T2:divaksin 75% dan 25% tidak divaksin) dengan sembilan kali ulangan. Faktor tambahanadalah waktu pengambilan serum (minggu ke-0, ke-1, ke-2 dan ke-3) sehingga jumlahsampel adalah 3x9x4= 108 sampel serum. Ayam umur 3 hari divaksinasi ND secara tetesmata kemudian dilakukan vaksinasi intramuskuler pada umur 21 hari sesuai perlakuan.Titer antibodi ND pada ayam perlakuan diuji dengan uji hambatanhemaglutinasi/hemagglutination inhibition (HI) satu hari sebelum vaksinasi, serta satuminggu, dua minggu, dan tiga minggu setelah vaksinasi. Data tentang titer antibodi (GMTHI)terhadap ND ditransformasi dengan akar X+1, dianalisis dengan sidik ragam dandilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa titerantibodi terhadap ND pada ayam yang tidak divaksin dipengaruhi oleh persentase ayamyang divaksin. Antibodi HI unit terhadap virus ND pada ayam yang tidak divaksinasimulai teramati pada minggu ke-2 dan ke-3 setelah vaksinasi. Titer antibodi ayam yangtidak divaksinasi pada kelompok ayam yang hanya divaksin 75% mempunyai titer antibodiyang nyata lebih tinggi dibandingkan dengan kelompok ayam yang divaksin 50% dankontrol (P<0,05). Pada kelompok ayam yang divaksin 50%, titer antibody ND pada ayamyang tidak divaksin secara statistik berbeda tidak nyata dibandingkan dengan kelompokyang divaksin 0% (P>0,05). Pada minggu ke tiga, titer antibody ND ayam yang tidakdivaksinasi pada kelompok ayam yang divaksin 75% nyata lebih tinggi dibandingkandengan pada kelompok ayam yang divaksin 50% (P,0,05). Vaksin ND aktif lentogeik LaSota dapat menyebar dari ayam yang divaksin secara intramuskuler kea yam yang tidakdivaksin

Seroprevalensi Penyakit Avian Influenza Pada Itik Di Kabupaten Klungkung Estry Gusnita Damanik; Gusti Ayu Yuniati Kencana; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.723 KB)

Itik memiliki peran penting dalam penyebaran virus Avian Influenza subtipe H5N1 karena merupakan reservoir alami virus dan infeksinya bersifat subklinis. Pendistribusian itik terjadi dari pasar unggas ke peternakan itik atau sebaliknya. Penelitian ini bertujuan untuk mengetahui perbedaan tingkat seroprevalensi virus Avian Influenza di Pasar Unggas Galiran dan peternakan itik di kabupaten Klungkung pada saat yang bersamaan. Sampel penelitian adalah serum dari itik yang tidak divaksin yang diambil dari pasar dan peternakan di kabupaten Klungkung. Pengambilan sampel dilakukan setiap bulan mulai bulan Maret sampai dengan bulan Agustus 2012. Sampel serum selanjutnya diuji dengan uji Hambatan Hemaglutinasi. Hasil penelitian menunjukkan seroprevalensi AI di Pasar Unggas Galiran dan peternakan itik di Kabupaten Klungkung adalah sebesar 81.4%, perbedaan yang signifikan terjadi pada Juni dan Agustus tetapi tidak signifikan pada bulan Maret, April, Mei, dan Juli. Seroprevalensi virus AI di Pasar Unggas Galiran adalah sebesar 76.2% dan di peternakan sebesar 86.7% dan secara statistik berbeda sangat nyata. Monitoring terhadap virus Avian Influenza berkelanjutan perlu dilakukan baik di peternakan maupun di pasar unggas di Klungkung.

Co-Authors Adi, Anak Agung Ayu Mira Agustin Zaharia Paderi Anak Agung Ayu Mirah Adi Anak Agung Sagung Kendran Andika Budi Kurnianto Anggreni, Ni Kadek Wiwik Aprilia Kusumastuti Aprillia Kusumastuti Arini Nur Handayani Arini Nur Handayani Arini Nurhandayani Arini Nurhandayani Baiq Indah Pertiwi Bhakty, Zatya Wira Charles Rangga Tabbu DERISNA SAWITRI UNGSYANI Estry Gusnita Damanik Fajar Mubarok G.A.M.K. Dewi Gusti Ayu Mayani Kristina Dewi Gusti Ngurah Narendra Putra Hamdani Y. Hartaputera, I Nyoman Surya Tri I GBA Purwanda I Gusti Agung Ayu Suartini I Gusti Ngurah Kade Mahardika I Ketut Eli Supartika I Ketut Suada I Ketut Tomy Caesar Ramanda I Komang Wahyu Yuliana I Made Kardena I Nyoman Dibia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Nyoman Sulabda I Putu Sudiarta I Putu Wira Adi Wibawa I Wayan Gorda I Wayan Masa Tenaya I wayan Teguh Wibawan I. A. P. Apsasri I. B. Ardana I. B. K. Suardana I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Suardana Kadek Karang Agustina Ketut Budiasa Ledi Natalia Surbakti Luh Dewi Anggreni Mesakh Parlindungan Simbolon Muh Ramadhan Musdalifa, Annisa Ni Made Ayu Sintya Paramita Ni Made Krisna Dewi Ni Wayan Apsari Shantika Pratistha Ni Wayan Intan Martinez Nirhayu, Nirhayu Nyoman Suartha Paranitha, Dewa Ayu Putu Dimas Abiyoga Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Putu Mira Puspitayani Raisis Farah Dzakiyyah Al-Aliyya Ratih, Dwi Kusuma Komala Remontara, Al Afuw Niha S.K. Widyastuti Silaban, Jesiaman Sri Kayati Widyastuti Steffi Ong Sumayani, N. K. E. Suwartini, Ni Komang Syamsidar . Syamsidar Syamsidar TRI KOMALA SARI Widya Asmara Widyasanti, Ni Wayan Helpina Wijaya, Dhyana Ayu Manggala Yuliantari, Ida Ayu Made

Title

Found 55 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 55 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 55 Documents
Search