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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Peternakan Tropika ARSHI Veterinary Letters Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences
Gusti Ayu Yuniati Kencana
Laboratorium Virologi Fakultas Kedokteran Hewan Universitas Udayana, Denpasar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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The Production and Use of Monoclonal Antibodies for the Detection of Avian Influenza Antigen in the in Infected Chickens Nyoman Mantik Astawa; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A safe and appropriate diagnostic method for avian influenza virus (AIV) infection in chickens wasestablished using monoclonal antibodies (mAbs) against the virus. The virus used for the production of themonoclonal antibodies was an Indonesian AIV-H5N1 isolate. Immortal mouse myeloma cells were fusedwith the lymphocytes derived from the spleen of mice immunized with the virus. The mAbs were tested fortheir specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehydeinactivated virus and normal allantoic fluid as antigens. Twelve mAbs specific against AIV were isolatedand 8 mAbs were used for immunodetection of AIV antigen in chicken’s tissues. By indirect ELISA, themAbs were able to detect AIV antigen in allantoic fluid at the titre as low as 2-2 to 2-4 HA units per 0.1 ml.By immunoperoxidase staining AIV–antigen was detected in paraffin embedded tissues of AIV-infectedchickens. AIV antigen was not detected in chickens which were confirmed to be AIV negative. In theinfected chickens, high intensity of AIV antigen was detected in proventricle gland and small intestine.The AIV antigen with a lesser intensity was detected in lungs and spleen but hardly detected in muscle,brain and several other tissues. This study show clear evidences that mAbs produced in this study areapplicable for use in the detection of AIV antigen in infected chickens.
Vaksin Kombinasi Newcastle Disease dengan Avian Influenza Memicu Imunitas Protektif pada Ayam Petelur terhadap Penyakit Tetelo dan Flu Burung (COMBINED NEWCASTLE DISEASE (ND) AND AVIAN INFLUENZA (AI) VACCINES INDUCE PROTECTIVE IMMUNE RESPONSE IN COMMERCIA Gusti Ayu Yuniati Kencana; I Nyoman Suartha; Ni Made Ayu Sintya Paramita; Arini Nur Handayani
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Newcastle disease (ND) and Avian Influenza (AI) are infectious diseases and still endemic in Indonesia.Prevention of the disease is conducted by vaccination of birds as the source of the infection. The use ofcombined ND-AI vaccine is expected to be able to prevent both diseases simultaneously. This study aimwas to determine the potency of combined ND-AI vaccine in field condition. Field trial vaccination wasconducted in commercial layer chickens in Tabanan Bali, and the HI test was conducted at the Faculty ofVeterinary Medicine Udayana University, Denpasar. Field trial in commercial layer chickens showed thatthe average HI titer of ND sera from pre-vaccinated chickens was 22.7HI units and AI titer was 21.27 HIunits. The ND titers increased to 25.47 HI Unit, 27.0 HI units, and to 28.73 HI units, whereas AI titersincreased to 27.93 HI Unit, 28.53 HI units, and 28.47 HI units in two, three and four weeks post-vaccinationwith the ND-AI combined vaccine, respectively. Statistically, based on ND and AI antibody pre and postvaccination,it is indicated that the combined ND-AI vaccine was able to induce immune response higherthan the protective titer level (>24). Period of collecting the sera samples also affected the titer of NDVand AI antibodies (P<0.01). Therefore it is recommended that vaccination should be conducted at antibodytiter of < 4 HI Unit.
Karaterisasi Virus Avian Influenza Subtipe H5N1 Isolat Lapang Asal Bali Untuk Kandidat Vaksin Gusti Ayu Yuniati Kencana; I Nyoman Suartha; I Made Kardena; Arini Nurhandayani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.103 KB) | DOI: 10.19087/jveteriner.2020.21.4.530

Abstract

A research on the isolation and characterization of the Avian Influenza H5N1 subtype field isolate has been carried out at the BSL-3 Laboratory of PT Sanbio Laboratories, Bogor. The aim of the study was to prepare a candidate for the H5N1 subtype Avian Influenza virus vaccine. Virus isolates were taken from field isolates from Bali. A total of seven field H5N1 AI subtypes from Bali were characterized in Bogor. The isolates were: isolate 3A, isolate 4A, isolate 9C, isolate 10 A, isolate 10 C, isolate P65, isolate P67. The passage of isolates was carried out on 9-day-old embryonic Specific Pathogenic (SPF) chicken eggs by injecting 0.1 mL of SPF isolates/eggs through the allantoic cavity. Each isolate was placed in five SPF eggs and then incubated in an incubator at 37 C and candled every day. Since day 2-4 post inoculation, embryo death has occurred. The eggs are harvested by their allantoic fluid and tested for haemagglutination test(HA/HI). The HI test results were confirmed by Reverse Transcriptase Polymerase Chain Reaction(RT-PCR) using the front primer FPHA232_13 (ATTGGTTAYCATGCAAAYAACTCG) and the back primer BPHA232_597 (GGAAAYATAGGTRGTTGGRTTYTGATAG) The results were five of the seven isolates were positive AI subtype B5 585 - 581 The five isolates of AI subtype H5N1 were subsequently sequenced, the results were all positive for AI virus subtype H5N1 clade 2.3.2. Each field isolate was given the name A / Chicken / Bali3A / GAY / 2019; A / Chicken / Bali9C / GAY / 2019; A / Chicken / BaliA4 / GAY / 2019; A / Chicken / Bali10A / GAY / 2016 and A / Chicken / Bali10C / GAY / 2019. One A / Chicken / Bali 9C / GAY / 2016 isolate was subsequently repeated 7 times until a stable H5N1 subtype AI virus titer was obtained. The results of matching with bioinformatics turned out that A / Chicken / Bali 9C / GAY / 2016 isolates had a kinship of 98.62% with AI subtype H5N1 Banyuwangi, amounting to 98.45% with AI subtype H5N1 Lamongan, amounting to 98.10% with AI-H5N1 Lumajang, 97.58% with AI-H5N1 Kediri, 97.07% with AIH5N1 Blitar, 96.72% with AI-H5N1 Denpasar, 96.72% with AI-H5N1 Buleleng and 96.72% with AI-H5N1Sukoharjo. The conclusion is one of isolate namely A / Chicken / Bali 9C / GAY / 2019 including AI subtype H5N1 clade 2.3.2, is’t stable at passage on SPF eggs, has a kinship of 96.72% with A / duck / Sukoharjo / BBVW-1428- 9/2012, the virus content is 106.9 ELD50 so it is potential for vaccine candidates.
Non Coding Region dan Amino Terminus Gen Polimerase Asidik Virus Avian Influenza Subtipe H5N1 Asal Hewan Indonesia Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The knowledge on the species adaptation factor of avian influenza virus of H5N1 subtype (AIV H5N1)is very important as a signal for the emergence of a new strain with pandemic potential. This research wasconducted to find out the sequence variation of the Non-Coding Region (NCR) and Coding Region (CR) of 5’-terminal cRNA of the polymerase acidic (PA). Total RNA from twenty six (26) avian influenza virussubtype H5N1 isolates were amplified using reverse-transcriptase-polymerase chain reaction (RT-PCR)with a universal forward primer for influenza virus and specifically designed backward primers. Nineteen(19) gene fragments of PA could be amplified. RT-PCR products were sequenced and analyzed using Mega4 software. The length of NCR of PA gene was found to be 24 bases and conserved. A/T composition of PANCR was 58.3%. Species and geographical specificity could not be found in the genetic distance, the aminoacid polymorphism, as well as the phylogenetic analysis of the CR. RNA sequencing is discussed andrecomended to be studied further.
Respons Antibodi terhadap Penyakit Tetelo pada Ayam yang Divaksin Tetelo dan Tetelo-Flu Burung (NEWCASTLE DISEASE/ND ANTIBODY RESPONSE OF CHICKENS VACCINATED WITH ND SINGLE AND COMBINED ND AND AVIAN INFLUENZA VACCINES) Gusti Ayu Yuniati Kencana; Nyoman Suartha; Mesakh Parlindungan Simbolon; Arini Nur Handayani; Steffi Ong; Syamsidar .; Aprillia Kusumastuti
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to investigate antibody response of specific pathogen-free (SPF) chickens vaccinatedwith single inactivated Newcastle disease (ND) vaccine and combined inactive ND and avian influenza(AI) vaccines and to known the efficacy of both vaccines. The vaccines used were killed ND vaccine andkilled ND-AI vaccine produced by PT. Sanbio Laboratories Bogor, West Java. SPF chickens were vaccinatedwith 3 different doses. Antibody titer of SPF chickens against ND virus were determined byhaemagglutination inhibition (HI) test. As many as 130 two week old SPF chickens were used and theywere divided into 2 groups (A and B) consisting of 60 chickens and 10 chickens were used as control withoutvaccine. Group A chickens were vaccinated with ND-K vaccine and group B were vaccinated with combinedkilled ND-AI vaccines. Each group was further divided into 3 subsgroups (1, 2 and 3) consisting 20 chickens.Subgroups 1, 2 and 3 were vaccinated intramuscularly respectively with intramuskular 1, 1/10 and 1/100doses of each vaccines. Antibody response of chickens against ND virus was examined before vaccinationand every three week after vaccination and was expresses as geometric mean titre (GMT) HI units. Theresult showed that the titre antibody against ND increased at the second week following the vaccination.The antibody titer against ND virus of chickens vaccinated single killed ND at the second week in eachdose were 6.05 GMT HI unit, 4.05 GMT HI unit, and 0.9 GMT HI unit. The antibody titre at the third week were 7.90 GMT HI unit ,5.40 GMT HI unit and 2.20 GMT HI unit. The antibody titre against ND virus ofchickens vaccinated with combined ND-AI vaccine at the second week were 6.30 GMT HI unit , 4.15 GMTHI unit , and 2.05 GMT HI unit. At the third week, the antibody titre against ND virus of chickensvaccinated with combined ND-AI vaccine in each subgroup were 7.45 GMT HI unit, 5.60 GMT HI unit , and2.40 GMT HI unit . It showed that the antibody titers at single doses of killed ND vaccine (7.90 GMT HIunit) and combined ND-AI vaccine (7.45 GMT HI unit) at the third week after vaccination were both stilleffective as both titres were above standard protective titre.
Monoclonal Antibodies as Ligands for Purificaion of Rabies virus Proteins from the Brain Tissues of Infected Dogs and Mice (ANTIBODI MONOCLONAL SEBAGAI LIGAND UNTUK PURIFIKASI PROTEIN VIRUS RABIES ASAL JARINGAN OTAK ANJING DAN MENCIT TERINFEKSI) Nyoman Mantik Astawa; Gusti Ayu Yuniati Kencana; Ida Bagus Suardana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Immunoaffinity chromatography using monoclonal antibodies (mAbs) as ligands has been used topurify rabies virus (RV) individual proteins. In this method, mAbs against RV were firstly purified,coupled to CnBr-agarose resin and used for purification RV individual proteins. Brain tissue homogenatesderived from infected and uninfected dogs and mice were mixed with mAbs-CnBr agarose resin and washedextensivelly phosphate buffered salin (PBS). Following elution and neutralization, purified proteins weredetected by enzyme-linked immunosirbent assay (ELISA) and Western blotting assay. Of the three mAbs(BB5, AE11 and AF6) as ligands, mAb AE11-CnBr agarose resin yielded highest protein levels as comparedto those of mAb BB5-CnBr agarose and mAb AF6-CnBr agarose resins. In Western Blotting assay, thepurified protein appeared to be 65 Kda (glycoprotein) and 38 kDa proteins. In ELISA test, the purifiedproteins reacted with both mAbs and policlonal antibodies (pAbs).
VARIATION OF NON-CODING REGION AND CODING REGION OF 5’-TERMINAL CRNA OF POLYMERASE BASIC 1 OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The sequence of the Non-Coding Region (NCR) and Coding Region (CR) of 5’-terminal cRNA of thepolymerase basic 1 (PB1) gene as a major factor for the species adaptation of avian influenza virussubtype H5N1 (AIV H5N1) has been analysed. The information could be a virological signal for theemergence of a new strain with pandemic potential. Total RNA from twenty six (26) avian influenzasubtype H5N1 isolates were amplified using reverse-transcriptase-polymerase chain reaction (RT-PCR)with a universal forward primer for influenza virus and specifically designed backward primers. Fifteen(15) PB1 gene fragments could be amplified. RT-PCR products were sequenced and analyzed using Mega4software. The length of NCR of PB1 gene was found to be 24 bases and mostly shows conserved sequence,with an exception of Dk/Badung/2006 isolate which has C-7T substitution. A/T composition of PB1 NCRwas 54,2%, while the Dk/Badung/2006 isolate was 58,3%. Species and geographical specificity could not befound in the genetic distance, the amino acid polymorphism, as well as the phylogenetic analysis of t
Pelacakan Kasus Flu Burung pada Ayam dengan Reverse Transcriptase Polymerase Chain Reaction* (DETECTION OF AVIAN INFLUENZA IN CHICKENS BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION) Gusti Ayu Yuniati Kencana; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana; I Nyoman Mantik Astawa; Ni Made Krisna Dewi; Gusti Ngurah Narendra Putra
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian Influenza (AI) or Bird Flu is a fatal zoonotic disease caused by highly pathogenic avian influenza(HPAI) virus of H5N1 sub-type. The disease is still endemic in Indonesia. This study was conducted toinvestigate AI cases in chickens in Bali. Virus isolation was performed in 9 day-old embryonated chickeneggs, and then followed by serologic testing by haemaglutination (HA) and Haemaglutination Inhibition(HI) assay using standard microtiter procedure. All of the samples were further tested with reversetrancriptasepolymerase chain reaction (RT-PCR). All work has been done in the Biomedical and MolecularBiology Laboratory, Faculty of Veterinary Medicine, Udayana University, Denpasar, during the period2009-2011. A total of ten samples were examined A total of ten chicken samples consisting of 6 fieldsamples and 4 meat samples have been confirmed to be AIV H5N1. All field cases showed clinical signsand gross pathology that were typical to the infection of avian influenza. The result indicates that AI casesare still prevalent among chickens in Bali.
KUALITAS TELUR AYAM ISA BROWN UMUR 18-22 MINGGU PASCA DIVAKSINASI EGG DROP SYNDROME DAN DIBERI RANSUM DALAM JUMLAH YANG BERBEDA Sumayani, N. K. E.; G. A. M. K. Dewi; G. A. Y. Kencana
Jurnal Peternakan Tropika Vol 7 No 1 (2019): Vol. 7 Isssues 1 (2019)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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Abstract

Egg drop syndrome causes a decrease in the quality and quantity of eggs so it does not benefit farmers. The research was conducted at the Faculty of Animal Science, Bukit Jimbaran Campus, and egg quality tested every week in the Poultry Laboratory of the Faculty of Animal Science, Sudirman Campus, Denpasar, which lasted for 5 weeks. The study sample was used by Isa Brown laying hens on an average age of 18 weeks. The study design used a completely randomized design (CRD) consisting of 3 treatments and 5 replications. Each replication consisted of 3 laying 1 control hens of Isa Brown so that the total chickens used were 45 tails.The treatment of this study was R1:Chicken Isa Brown without a vaccine candidate and given a commercial ration of 80 g/head/day, R2:Chicken with candidates for the EDS vaccine and given a commercial ration of 80 g/head/day R3:Chicken with candidates vaccine EDS and given 84 g/head/day of commercial ration. The variables observed were egg weight, egg index, shell weight, shell thickness, egg yolk color, pH and, haugh unit. The results showed that treatment R2 and R3 (P<0,05) were able to increase egg weight, eggshell weight, eggshell thickness, egg yolk color and haugh unit compared to treatment R0 but there was a decrease in egg shape index, egg pH on R2 treatment. Based on the results of the study it can be concluded that the quality and quantity of R3 eggs is higher than the treatment of R2 in chiken egss Isa Brown aged 18-22 week. Keywords : Isa Brown, quality of eggs, rations, vaccine candidates, egg drop syndrome
Seroprevalensi dan Deteksi Antigen Virus Newcastle Disease (ND) Pada Ayam Buras di Kecamatan Lembar, Kabupaten Lombok Barat Hamdani Y.; G. A. Y. Kencana
Jurnal Peternakan Tropika Vol 6 No 1 (2018): Vol. 6 No. 1 (2018)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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Abstract

This study to determine seroprevalence of newcastle disease (ND) virus and detection of antigen on domestic chicken in district lembar, west lombok regency. A total of 6 (six) villages, serve as research sites namely Labuan Tereng Village, Lembar Selatan Village, Lembar Village, Jembatan Kembar Village, East Jembatan Kembar Village, and Nyiur Lembang Village. The samples were sera cloaca swab and tracheal swab taken by simple random sampling. A total of 150 poultry chickens each were taken sera, cloaca swab, and tracheal swab so that the number of samples to 150 sera samples, 150 samples of cloaca swabs, and 150 tracheal samples. Sera was examined by serological test of haemagglutination inhibition (HI) test while antigen detection was performed by hemagglutination (HA) test followed by Reverse Transcriptase Polymrase Chain Reaction (RT-PCR) test. Serology test results from 150 sera samples showed as many as 50 sera samples (33%) positive ND with titer of 22-29 HI units. While the antigen test results from 15 pooled samples showed as many as four positive samples of HA test and RT-PCR test showed a positive four samples with 380bp base length. It is concluded that ND virus infections have occurred in dstrict Lembar, West Lombok regency which hasbeen officially not yet reported. Keywords: Seroprevalence, antigen detection, Newcastle Disease, domestic chicken, West Lombok.
Co-Authors Adi, Anak Agung Ayu Mira Agustin Zaharia Paderi Anak Agung Ayu Mirah Adi Anak Agung Sagung Kendran Andika Budi Kurnianto Anggreni, Ni Kadek Wiwik Aprilia Kusumastuti Aprillia Kusumastuti Arini Nur Handayani Arini Nur Handayani Arini Nurhandayani Arini Nurhandayani Baiq Indah Pertiwi Bhakty, Zatya Wira Charles Rangga Tabbu DERISNA SAWITRI UNGSYANI Estry Gusnita Damanik Fajar Mubarok G.A.M.K. Dewi Gusti Ayu Mayani Kristina Dewi Gusti Ngurah Narendra Putra Hamdani Y. Hartaputera, I Nyoman Surya Tri I GBA Purwanda I Gusti Agung Ayu Suartini I Gusti Ngurah Kade Mahardika I Ketut Eli Supartika I Ketut Suada I Ketut Tomy Caesar Ramanda I Komang Wahyu Yuliana I Made Kardena I Nyoman Dibia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Nyoman Sulabda I Putu Sudiarta I Putu Wira Adi Wibawa I Wayan Gorda I Wayan Masa Tenaya I wayan Teguh Wibawan I. A. P. Apsasri I. B. Ardana I. B. K. Suardana I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Suardana Kadek Karang Agustina Ketut Budiasa Ledi Natalia Surbakti Luh Dewi Anggreni Mesakh Parlindungan Simbolon Muh Ramadhan Musdalifa, Annisa Ni Made Ayu Sintya Paramita Ni Made Krisna Dewi Ni Wayan Apsari Shantika Pratistha Ni Wayan Intan Martinez Nirhayu, Nirhayu Nyoman Suartha Paranitha, Dewa Ayu Putu Dimas Abiyoga Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Putu Mira Puspitayani Raisis Farah Dzakiyyah Al-Aliyya Ratih, Dwi Kusuma Komala Remontara, Al Afuw Niha S.K. Widyastuti Silaban, Jesiaman Sri Kayati Widyastuti Steffi Ong Sumayani, N. K. E. Suwartini, Ni Komang Syamsidar . Syamsidar Syamsidar TRI KOMALA SARI Widya Asmara Widyasanti, Ni Wayan Helpina Wijaya, Dhyana Ayu Manggala Yuliantari, Ida Ayu Made