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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Peternakan Tropika ARSHI Veterinary Letters Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences

Gusti Ayu Yuniati Kencana

Laboratorium Virologi Fakultas Kedokteran Hewan Universitas Udayana, Denpasar

Author-ID : 425703

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Identifikasi Secara Serologi Galur Virus Flu Burung Subtipe H5N1 Clade 2.1.3 dan Clade 2.3.2 pada Ayam Petelur (SEROLOGICAL IDENTIFICATION OF AVIAN INFLUENZA STRAIN VIRUS SUBTYPE H5N1 CLADE 2.1.3 AND CLADE 2.3.2 FROM LAYER) Aprilia Kusumastuti; Syamsidar .; Agustin Zaharia Paderi; Arini Nurhandayani; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the study was to know avian influenza (AI) infection in field by using serology test in threemarketing area of AI vaccines. Haemagglutination inhibition methode was used in this test. There werefour antigen strains of AI subtype H5N1 clade 2.1.3 (AIstrainA/Chicken/West Java/PWT-WIJ/2006, AIstrain A/Chicken/Garut/BBVW-223/2007, AI strain A/Chicken/West Java-Nagrak/30/2007, and AI strainA/Chicken/Pekalongan/BBVW-208/2007) and 2 antigen strains of AI subtype H5N1 clade 2.3.2 (AI strainA/duck/Sukoharjo/BBVW-1428-9/2012 and AI strain A/duck/Sleman/BBVW-1463-10/2012) was used inthis study for HI test. The result presents that 93,33% chicken farms in three marketing area of PT. SanbioLaboratories have positive antibody titre to AI subtype H5N1 clade 2.1.3. This titre may be obtained fromAI clade 2.1.3 vaccination. From 15 samples, 92,86% are positive to AI subtype H5N1 clade 2.3.2A/duck/Sukoharjo/BBVW-1428-9/2012 and 92,31% are positive to A/duck/Sleman/BBVW-1463-10/2012 evenwithout AI clade 2.3.2 vaccination. This antibody titre may be obtained from AI clade 2.1.3 vaccine crossprotection or field infection.

Seroprevalensi Flu Burung Subtipe H5N1 pada Itik Bali di Pasar Hewan Beringkit dan Pasar Umum Galiran, di Bali DERISNA SAWITRI UNGSYANI; Gusti Ayu Yuniati Kencana; I Wayan Masa Tenaya
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Flu burung atau Avian influenza (AI) subtipe H5N1, termasuk kelompok Highly Pathogenic Avian Influenza (HPAI) yang masih mengancam industri perunggasan, kesehatan manusia, dan sejumlah spesies burung liar. Itik merupakan reservoir alami virus AI H5N1 dan diduga kuat dapat menularkan virus ke unggas lain maupun manusia. Penelitian ini bertujuan untuk mengetahui seroprevalensi virus AI subtipe H5N1 pada itik bali di dua pasar yang menjual hewan hidup yaitu Pasar Hewan Beringkit di Kabupaten Badung dan Pasar Umum Galiran di Klungkung, Bali. Total sampel yang diambil sebanyak 120 serum itik bali, yang diambil dari itik umur lebih dari tiga bulan dan tidak divaksinasi AI subtipe H5N1. Pengambilan sampel dilakukan sebanyak empat kali dengan interval waktu dua minggu dari masing-masing pasar tersebut. Semua sampel serum tersebut selanjutnya diuji dengan uji Hambatan Hemaglutinasi (HI). Hasil uji menunjukan bahwa seroprevalensi AI subtipe H5N1 di Pasar Hewan Beringkit sebesar 20,0%, sedangkan di Pasar Umum Galiran sebesar 23,3%. Hasil ini mengindikasikan bahwa itik bali yang disampling dari kedua pasar tersebut pernah terpapar virus AI H5N1 secara alami. Surveilans dan monitoring serta tindakan pencegahan secara berkala perlu dilakukan untuk mencegah penyebaran penyakit AI H5N1 di pasar hewan.

AMINO-TERMINUS OF POLYMERASE BASIC-2 OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM VARIOUS ANIMAL SPECIES IN INDONESIA Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The information on pathogenicity and adaptation factors of avian influenza virus (AIV) in mammalsis very inportant in an effort to reduce the risk of avian influenza (AI) pandemic in the future. Polymerasegene complex appears to be the major factors for adaptation of AIV to certain animal species. A preliminarystudy on role of non-coding region (NCR) and amino-terminus of polymerase-basic 2 (PB2) is presented.Purified viral RNA of AIV isolated from chicken, duck, pig, and quail of Bali and Yogyakarta was reversetranscribed into cDNA and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR)using PB-2 universal forward primer and specifically designed backward primer. The result showed thatall AIV’s H5N1 isolated from chicken, duck, quail, and pig, posed PB2 amino-terminus typical for IndonesianAIV H5N1. However, polymorphic amino acids of the protein fragment did not show any species specificmotive, with the exception of the pig isolate Sw/Tabanan/2006 which had specific substitution of D16E,H17Q, M40I, and H124Y.

Deteksi Virus Avian Influenza Subtipe H5N1 pada Itik di Pasar Hewan Beringkit dan Pasar Galiran, Bali Ni Wayan Intan Martinez; Gusti Ayu Yuniati Kencana; I Nyoman Dibia
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Avian Influenza merupakan penyakit zoonosis disebabkan oleh virus Avian Influenza (AI) subtipe H5N1, penyakit ini ditemukan hampir di seluruh belahan dunia. Itik adalah unggas air yang merupakan inang alami virus AI. Virus AI subtipe H5N1 yang menyerang unggas dapat ditemukan pada unggas hidup yang dijual di pasar. Pasar hewan berperan penting dalam penyebaran virus AI dari unggas ke unggas, serta dari unggas ke manusia. Tujuan penelitian ini adalah untuk mengetahui sirkulasi virus AI pada itik di Pasar Hewan Beringkit dan Pasar Galiran, Bali. Total sampel 120 swab kloaka dan trakea itik diambil di pasar hewan Beringkit dan Galiran masing-masing 60 sampel. Sampel swab digabung (pooling) berdasarkan pedagang itik dan waktu pengambilan. Isolasi virus dilakukan pada telur ayam bertunas (TAB) berumur sembilan hari. Carian allantois yang dipanen selanjutnya diuji serologis hemaglutinasi (HA/HI) dan uji Reverese Transkriptase Polymerase Chain Reaction. Data hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan masing-masing satu sampel positif virus AI subtipe H5N1 dari setiap pasar dengan proporsi positif di kedua pasar sebesar 1,7% (2/120). Data ini mengindikasikan bahwa virus AI subtipe H5N1 masih bersirkulasi di Pasar Hewan Beringkit dan Galiran.

Respons Antibodi Sekunder Terhadap Penyakit Tetelo pada Ayam Petelur Pascavaksinasi Ulangan dengan Vaksin Tetelo Aktif (NEWCASTLE DISEASESECONDARY ANTIBODY RESPONSE AFTER REVACCINATION IN LAYER WITH THE ACTIVE ND VACCINE) Andika Budi Kurnianto; Gusti Ayu Yuniati Kencana; I Nyoman Mantik Astawa
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Revaccination is required in order to preventNewcastle Disease (ND) reccurence inlayers chickens. Oneof vaccine for ND revaccination is freeze-died ND active vaccine containing e” 106,5EID50. Revaccinationisdone to trigger a faster secondary antibody responses in layers and can achieve protective antibody titersagainst ND that can be monitored by a hemagglutinationinhibition (HI). The aim of this study was todetermine the ND secondary antibody responses in layers after revaccination with ND active vaccine.Antibody titer of 20 layers chickens of 20 week old were determined before revaccinations (week 0) andafter revaccinations (week 1 until week 9). The first vaccination was conducted using ND-IB (NewcastleDisease-Infectious Bronchitis) at the age of 2 days through eye drops and subcutaneous injection at the ageof 5 days using a dose of 1 ampoule.Vaccination is repeated at the age of 20 weeks at a dose of 1 ½ ampoule through drinking water. Blood samples were collected on the wing vein (venous cutane ulnar) and left for 5-10 minutes at room temperature.Sera were then collected and stored at -20oC until use. HI antibody titerwas determined by micro titeration system. The HI mean titers were analyzed by Duncan test. The studyresults showed that antibody titer before revaccination was3,47 HI log 2 and the HI titers after revaccinationwere 4,02; 5,22; 6,52; 7,85; 8,4; 8,6; 7,7; 5,92; dan 3,87 HI log 2 respectivelly at weeks 1, 2, 3, 4, 5, 6, 7, 8, and9.The NDV revaccination with ND active vaccine significantly (P <0.01) increased in antibody titer inlayers starting from week 1 to week 6, but decreased following week 7 to week-9. It can be concluded thatrevaccinantion with ND active vaccine increases the antibody titers in layer chickens.

Vaksin Gumboro Menyebabkan Imunosupresif pada Respons Primer Vaksin Penyakit Tetelo Ayam Pedaging Gusti Ayu Yuniati Kencana; Anak Agung Ayu Mirah Adi; Ida Bagus Komang Ardana; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The variety of Gumboro live vaccine strains (hot, intermediate, and mild) are available inIndonesia. The immunosuppresive effect of these vaccines under field conditions is not known.This research was conducted to determine this devastating effect of such vaccine strains on theimmune response of chickens vaccinated againts Newcastle disease (ND). Sixty chickens werekept separately in five groups (i.e. V1, V2, V3, V4, and K). At the age of seven days, group V1, V2,and V3 were given hot, intermediate, and mild strains of Gumboro live vaccine respectively whilethe other two groups recieved no Gumboro vaccine (V4 and K). At the age of 14 days, all groups,except group K which were kept as a negative control, were vaccinated against ND. The level ofantibody produced in response to ND vaccination was measured in sera collected at day 0, 7, 14,and 21 post ND vaccination using a standard micro-haemaglutination inhibition test. Data of theantibody titers were analyzed using analysis of variance followed by Duncan’s multiple range test.The results showed that all Gumboro vaccine strains still retain its immunosuppressive nature onhumoral immune response in chickens that later vaccinated against ND. The geometric meantiter (GMT) of anti-NDV antibody of group V4 (unvaccinated againts Gumboro) was significantlyhigher than that of group V1, V2, and V3, i.e. groups of chickens that had been given varietystrains of Gumboro vaccines, at the first and second week after ND vaccination (p<0.05). Thedifference of this immunosuppressivenes among variety of Gumboro vaccine strains need furtherclarification.

Perbandingan Sekuens Konsensus Gen Hemaglutinin Virus Avian Influenza Subtipe H5N1 Asal Unggas di Indonesia dengan Subtipe H5N2 dan H5N9 I Gusti Ngurah Kade Mahardika; I Nyoman Suartha; Ida Bagus Kade Suardana; I Gusti Ayu Yuniati Kencana; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Consensus sequence of hemagglutinin (HA) gene of avian influenza viruses of H5N1 subtype isolatedfrom fowl in Indonesia – hereafter named as H5N1_Indonesia – is compared with that of H5N2 and H5N9viruses. Sequence information were downloaded from the public database GeneBank. The genetic distancesand nucleotide as well as its deduced amino-acid sequence alignment were analysed. At nucleotide level,genetic distances of HA between H5N1_Indonesia to H5N2 and H5N9 are 16.2% and 9.6%, respectively.At amino-acid level, the distances are 9.7% and 6.8%. The genetic distances of HA1 fragments are 19.0%(H5N1_Indonesia – H5N2) and 10.9% (H5N1_Indonesia – H5N9). At amino-acid, level the genetic distancesof HA1 are 13.1% (H5N1_Indonesia-H5N2) and 8.8% (H5N1_Indonesia – H5N9). All three subtypes havedifferent glycosilation motive and variation of amino-acid sequence of four antigenic sites. The consequenceof those facts is discussed.

The Production and Use of Monoclonal Antibodies for the Detection of Avian Influenza Antigen in the in Infected Chickens Nyoman Mantik Astawa; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A safe and appropriate diagnostic method for avian influenza virus (AIV) infection in chickens wasestablished using monoclonal antibodies (mAbs) against the virus. The virus used for the production of themonoclonal antibodies was an Indonesian AIV-H5N1 isolate. Immortal mouse myeloma cells were fusedwith the lymphocytes derived from the spleen of mice immunized with the virus. The mAbs were tested fortheir specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehydeinactivated virus and normal allantoic fluid as antigens. Twelve mAbs specific against AIV were isolatedand 8 mAbs were used for immunodetection of AIV antigen in chicken’s tissues. By indirect ELISA, themAbs were able to detect AIV antigen in allantoic fluid at the titre as low as 2-2 to 2-4 HA units per 0.1 ml.By immunoperoxidase staining AIV–antigen was detected in paraffin embedded tissues of AIV-infectedchickens. AIV antigen was not detected in chickens which were confirmed to be AIV negative. In theinfected chickens, high intensity of AIV antigen was detected in proventricle gland and small intestine.The AIV antigen with a lesser intensity was detected in lungs and spleen but hardly detected in muscle,brain and several other tissues. This study show clear evidences that mAbs produced in this study areapplicable for use in the detection of AIV antigen in infected chickens.

Vaksin Kombinasi Newcastle Disease dengan Avian Influenza Memicu Imunitas Protektif pada Ayam Petelur terhadap Penyakit Tetelo dan Flu Burung (COMBINED NEWCASTLE DISEASE (ND) AND AVIAN INFLUENZA (AI) VACCINES INDUCE PROTECTIVE IMMUNE RESPONSE IN COMMERCIA Gusti Ayu Yuniati Kencana; I Nyoman Suartha; Ni Made Ayu Sintya Paramita; Arini Nur Handayani
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Newcastle disease (ND) and Avian Influenza (AI) are infectious diseases and still endemic in Indonesia.Prevention of the disease is conducted by vaccination of birds as the source of the infection. The use ofcombined ND-AI vaccine is expected to be able to prevent both diseases simultaneously. This study aimwas to determine the potency of combined ND-AI vaccine in field condition. Field trial vaccination wasconducted in commercial layer chickens in Tabanan Bali, and the HI test was conducted at the Faculty ofVeterinary Medicine Udayana University, Denpasar. Field trial in commercial layer chickens showed thatthe average HI titer of ND sera from pre-vaccinated chickens was 22.7HI units and AI titer was 21.27 HIunits. The ND titers increased to 25.47 HI Unit, 27.0 HI units, and to 28.73 HI units, whereas AI titersincreased to 27.93 HI Unit, 28.53 HI units, and 28.47 HI units in two, three and four weeks post-vaccinationwith the ND-AI combined vaccine, respectively. Statistically, based on ND and AI antibody pre and postvaccination,it is indicated that the combined ND-AI vaccine was able to induce immune response higherthan the protective titer level (>24). Period of collecting the sera samples also affected the titer of NDVand AI antibodies (P<0.01). Therefore it is recommended that vaccination should be conducted at antibodytiter of < 4 HI Unit.

Karaterisasi Virus Avian Influenza Subtipe H5N1 Isolat Lapang Asal Bali Untuk Kandidat Vaksin Gusti Ayu Yuniati Kencana; I Nyoman Suartha; I Made Kardena; Arini Nurhandayani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

A research on the isolation and characterization of the Avian Influenza H5N1 subtype field isolate has been carried out at the BSL-3 Laboratory of PT Sanbio Laboratories, Bogor. The aim of the study was to prepare a candidate for the H5N1 subtype Avian Influenza virus vaccine. Virus isolates were taken from field isolates from Bali. A total of seven field H5N1 AI subtypes from Bali were characterized in Bogor. The isolates were: isolate 3A, isolate 4A, isolate 9C, isolate 10 A, isolate 10 C, isolate P65, isolate P67. The passage of isolates was carried out on 9-day-old embryonic Specific Pathogenic (SPF) chicken eggs by injecting 0.1 mL of SPF isolates/eggs through the allantoic cavity. Each isolate was placed in five SPF eggs and then incubated in an incubator at 37 C and candled every day. Since day 2-4 post inoculation, embryo death has occurred. The eggs are harvested by their allantoic fluid and tested for haemagglutination test(HA/HI). The HI test results were confirmed by Reverse Transcriptase Polymerase Chain Reaction(RT-PCR) using the front primer FPHA232_13 (ATTGGTTAYCATGCAAAYAACTCG) and the back primer BPHA232_597 (GGAAAYATAGGTRGTTGGRTTYTGATAG) The results were five of the seven isolates were positive AI subtype B5 585 - 581 The five isolates of AI subtype H5N1 were subsequently sequenced, the results were all positive for AI virus subtype H5N1 clade 2.3.2. Each field isolate was given the name A / Chicken / Bali3A / GAY / 2019; A / Chicken / Bali9C / GAY / 2019; A / Chicken / BaliA4 / GAY / 2019; A / Chicken / Bali10A / GAY / 2016 and A / Chicken / Bali10C / GAY / 2019. One A / Chicken / Bali 9C / GAY / 2016 isolate was subsequently repeated 7 times until a stable H5N1 subtype AI virus titer was obtained. The results of matching with bioinformatics turned out that A / Chicken / Bali 9C / GAY / 2016 isolates had a kinship of 98.62% with AI subtype H5N1 Banyuwangi, amounting to 98.45% with AI subtype H5N1 Lamongan, amounting to 98.10% with AI-H5N1 Lumajang, 97.58% with AI-H5N1 Kediri, 97.07% with AIH5N1 Blitar, 96.72% with AI-H5N1 Denpasar, 96.72% with AI-H5N1 Buleleng and 96.72% with AI-H5N1Sukoharjo. The conclusion is one of isolate namely A / Chicken / Bali 9C / GAY / 2019 including AI subtype H5N1 clade 2.3.2, is’t stable at passage on SPF eggs, has a kinship of 96.72% with A / duck / Sukoharjo / BBVW-1428- 9/2012, the virus content is 106.9 ELD50 so it is potential for vaccine candidates.

Co-Authors Adi, Anak Agung Ayu Mira Agustin Zaharia Paderi Anak Agung Ayu Mirah Adi Anak Agung Sagung Kendran Andika Budi Kurnianto Anggreni, Ni Kadek Wiwik Aprilia Kusumastuti Aprillia Kusumastuti Arini Nur Handayani Arini Nur Handayani Arini Nurhandayani Arini Nurhandayani Baiq Indah Pertiwi Bhakty, Zatya Wira Charles Rangga Tabbu DERISNA SAWITRI UNGSYANI Estry Gusnita Damanik Fajar Mubarok G.A.M.K. Dewi Gusti Ayu Mayani Kristina Dewi Gusti Ngurah Narendra Putra Hamdani Y. Hartaputera, I Nyoman Surya Tri I GBA Purwanda I Gusti Agung Ayu Suartini I Gusti Ngurah Kade Mahardika I Ketut Eli Supartika I Ketut Suada I Ketut Tomy Caesar Ramanda I Komang Wahyu Yuliana I Made Kardena I Nyoman Dibia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Nyoman Sulabda I Putu Sudiarta I Putu Wira Adi Wibawa I Wayan Gorda I Wayan Masa Tenaya I wayan Teguh Wibawan I. A. P. Apsasri I. B. Ardana I. B. K. Suardana I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Suardana Kadek Karang Agustina Ketut Budiasa Ledi Natalia Surbakti Luh Dewi Anggreni Mesakh Parlindungan Simbolon Muh Ramadhan Musdalifa, Annisa Ni Made Ayu Sintya Paramita Ni Made Krisna Dewi Ni Wayan Apsari Shantika Pratistha Ni Wayan Intan Martinez Nirhayu, Nirhayu Nyoman Suartha Paranitha, Dewa Ayu Putu Dimas Abiyoga Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Putu Mira Puspitayani Raisis Farah Dzakiyyah Al-Aliyya Ratih, Dwi Kusuma Komala Remontara, Al Afuw Niha S.K. Widyastuti Silaban, Jesiaman Sri Kayati Widyastuti Steffi Ong Sumayani, N. K. E. Suwartini, Ni Komang Syamsidar . Syamsidar Syamsidar TRI KOMALA SARI Widya Asmara Widyasanti, Ni Wayan Helpina Wijaya, Dhyana Ayu Manggala Yuliantari, Ida Ayu Made

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