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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Biospecies Biotika: Jurnal Ilmiah Biologi Bioscience Bioeducation Journal Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) AL KAUNIYAH Jurnal Peternakan Indonesia Jurnal Pendidikan Biologi Indonesia BIOMA Jurnal Penelitian Sains BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi JKTP: Jurnal Kajian Teknologi Pendidikan BIOEDUSCIENCE Jurnal Biologi Tropis ICoTE Proceedings JURNAL PENGABDI PENDIPA Journal of Science Education Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Window of Health : Jurnal Kesehatan BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Bioma : Jurnal Biologi dan Pembelajaran Biologi JPBIO (Jurnal Pendidikan Biologi) Bioedusiana: Jurnal Pendidikan Biologi GERVASI: Jurnal Pengabdian kepada Masyarakat Jurnal Pengabdian Dharma Laksana JURNAL BIOEDUCATION Jurnal Mangifera Edu Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Reswara: Jurnal Pengabdian Kepada Masyarakat Jurnal Sains dan Edukasi Sains IJPD (International Journal Of Public Devotion) Bioscientist : Jurnal Ilmiah Biologi Integrated Science Education Journal Jurnal ABM Mengabdi Oryza : Jurnal Pendidikan Biologi BIOMA : Jurnal Biologi dan Pembelajarannya TROPICAL GENETICS EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Quagga: Jurnal Pendidikan dan Biologi Tropical Genetics Unnes Science Education Journal Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi Jurnal Perikanan Jurnal Pendidikan MIPA Jurnal Info Kesehatan IJIS Edu : Indonesian Journal of Integrated Science Education Arwana: Jurnal Ilmiah Program Studi Perairan Biodiversitas Journal of Biological Diversity
WOLLY CANDRAMILA
Universitas Tanjungpura
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Journal : tropical genetics

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Identification of single nucleotide polymorphisms on the D-loop region of mtDNA in Sundanese population Wolly Candramila; Sony Heru Sumarsono; Bambang Suryobroto; Maelita Ramdani Moeis
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Identification of sequence polymorphism on the D-loop region of mtDNA has been done for various purposes, including health and medical treatment. In this research, single nucleotide polymorphisms were identified in the D-loop region of mtDNA of the Sundanese population in western Java. A total of 118 unrelated and healthy Sundanese probands were collected from closed-traditional kampung adat and open communities distributed in 14 cities and regencies in western Java. DNA amplification and direct sequencing of the D-loop region were proceeded using primers L15990 and H409. Multi-alignment was conducted not only intrapopulation but also with D-loop sequence data stored in GenBank for comparison. In this research, we categorized high-frequency SNPs as less effective for identification in population studies because of their presence in other populations outside Indonesia. Meanwhile, lower-frequency SNPs showed typical variants of Sundanese haplotypes. On the other hand, rare or low-frequency SNPs should be re-examined in larger size of samples to have a better understanding of risk factors for many diseases.
Maternal genetic variation of Betawi population Wolly Candramila; Bambang Suryobroto
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Betawi identity was assessed based on the diversity of maternal lines using genetic markers in the D-loop region of mitochondrial DNA. The results of the cleavage by the restriction enzymes DdeI, HaeII, HaeIII, and HinfI showed five patterns of genetic variants which were later named Haplotype I, II, III, IV, and V. Haplotype I had the highest frequency of 75.6%, followed by Haplotype III 11.5%, and Haplotype II 10.3%. Haplotypes IV and V were only found in 1 individual each and were assumed to be individual mutations. The highest haplotype in the Betawi population showed the same restriction enzyme cleavage pattern with all comparison samples from the Sunda and Malay populations. Meanwhile, Haplotype II and III were strongly suspected as the result of the divergence of Haplotype I and can also be found in other ethnic groups in Indonesia. This finding confirms Jakarta as a location for ethnic mixing which then produces a new local culture as Betawi Culture.
Phylogenetic and Genetic Distance Analysis of the Mangrove Worm (Namalycastis) Based on the 18S rRNA Gene Using in Silico Methods Arielda Putri Aditya; Junardi Aditya; Wolly Candramila; Kartika Prabasari
Tropical Genetics Vol. 5 No. 2 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i2.100

Abstract

Namalycastis is a polychaete worm that inhabits dynamic aquatic environments and exhibits high genetic and morphological variability. This study investigates the phylogeny of the genus Namalycastis through an in silico analysis using the 18S ribosomal RNA (rRNA) gene. This genetic marker is considered ideal as it combines conserved and variable regions, both essential for taxonomic and evolutionary analyses. The objective of this research was to examine the phylogenetic relationships and genetic distances among 12 species of the genus Namalycastis based on their 18S rRNA gene sequences. DNA sequences were obtained from the NCBI database and aligned using Clustal-W in MEGA XI software. Phylogenetic reconstruction was performed using the Neighbor-Joining method with 1000 bootstrap replications, applying the Kimura 2-Parameter (K2P) model. The results revealed that Namalycastis jaya shares a very close evolutionary relationship with Namalycastis abiuma, forming a monophyletic clade that is distinct from Namalycastis hawaiiensis. The genetic distances among N. jaya, N. abiuma, and N. abiuma group sp. indicate a close evolutionary affinity, whereas N. hawaiiensis displays greater genetic divergence from the other two species. Overall, this study demonstrates that the 18S rRNA gene is an effective molecular marker for identifying phylogenetic relationships among Namalycastis species. The findings also highlight the potential of in silico methods in elucidating evolutionary patterns within the genus Namalycastis.
Evaluating Misconceptions in the Representation of Mendelian Genetic Inheritance in High School Biology Textbooks Kusniawati Kusniawati; Wolly Candramila; Eko Sri Wahyuni
Tropical Genetics Vol. 5 No. 2 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i2.107

Abstract

Genetics learning is prone to misconceptions due to complex concepts, abstract reasoning, and inconsistencies in textbook presentations, which can hinder students’ understanding of inheritance patterns such as Mendelian genetics. This study analyzes the potential misconceptions in high school biology textbooks regarding Mendel’s Laws and dominance–recessive inheritance using Dikmenli et al.’s categorization, aiming to provide references for teachers in selecting and supplementing instructional materials. This is a descriptive qualitative research using content analysis method. Four textbooks, selected through a survey of biology teachers in Pontianak City, were analyzed by comparing key concepts in the textbooks with scientifically accurate explanations from authoritative references. The findings were systematically examined, categorized, and validated through member-checking to ensure accuracy, highlighting the most common types of misconceptions in the textbooks. The analysis revealed variations in the coverage and accuracy of 13 key concepts related to Mendel’s Laws and dominance–recessive inheritance, with Books A–C containing several potential misconceptions while Book D showed none. The most frequent types of misconceptions identified were misidentifications, followed by oversimplifications and overgeneralizations, often involving definitions of gene, genotype, phenotype, and independent assortment. These findings highlight the importance of accurate textbook content and suggest that educators must critically evaluate and clarify textbook explanations to prevent persistent misunderstandings in students’ conceptual understanding of genetics.
Identification and Categorization of Misconceptions on Mutations in Highschool Biology Textbooks Mohammad Alfin Rizkiandi; Wolly Candramila; Andi Besse Tenriawaru
Tropical Genetics Vol. 6 No. 1 (2026): Tropical Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v6i1.130

Abstract

This study examined misconceptions found in Grade XII Biology textbooks, specifically in the topic of mutation. A qualitative descriptive approach was employed using content analysis on four Biology textbooks commonly used in senior high schools in Pontianak. Key concepts were identified based on Core Competency 3.8 and compared with credible references from genetic literature. Potential misconceptions were verified by two experts and categorized into five types of errors according to the classification by Dimenli, Cardak, and Oztas: misidentification, oversimplification, overgeneralization, undergeneralization, and outdated concepts or terminology. Data validity was ensured through triangulation techniques involving teacher interviews, textbook content analysis, and documentation. The results indicate that two out of the four textbooks analyzed contain several misconceptions in presenting mutation material, ranging from definitions and types of gene mutations to chromosomal mutations. These findings highlight the importance of evaluating textbook content as a primary learning resource to prevent conceptual misunderstandings among students. This research is expected to serve as a reference for teachers and students in selecting and utilizing Biology textbooks more critically.
Identification and Characterization of Genetic Variants in the Human ACAN Gene Using the ClinVar Database Arielda Putri Aditya; Wolly Candramila
Tropical Genetics Vol. 6 No. 1 (2026): Tropical Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v6i1.138

Abstract

The ACAN gene plays an important role in skeletal growth and development, so mutations in this gene are associated with various disorders such as short stature and skeletal dysplasia. This study aims to identify and characterize ACAN gene mutation variations using the ClinVar database at the National Center for Biotechnology Information to support the interpretation of pathogenicity and genetic diagnosis. This study was conducted in silico with a descriptive-comparative approach using ACAN gene variant data from the ClinVar database at the National Center for Biotechnology Information. Pathogenic and likely pathogenic variants were analyzed and grouped based on germline classification, molecular consequence, and variation type. The results of the ClinVar database analysis at the National Center for Biotechnology Information showed that most ACAN gene variants are categorized as pathogenic, with missense variants, frameshift variants, and single-nucleotide variants as the most dominant mutation types found. Genetic variations in the ACAN gene have a strong clinical association with skeletal growth disorders, especially short stature, so that ACAN gene variant analysis has the potential to support genetic diagnosis, prognosis, and genetic counselling in skeletal disorders. This study also shows that the use of bioinformatics databases can help understand the relationship between genetic variation and disease more systematically.
Co-Authors Abdillah, Dani Ade Mirza Adrianto Oktavianus Afandi Afandi Afriany, Messy Agung Mulyono Aini Lestari Akbar, Didi Alda, Alda Aldiansyah, Rifki Alexandra Jeni Yoesianata Andi Besse Tenriawaru Andri Maulidi Anisyah Yuniarti Anisyah Yuniarti Annisafira Nanda Rosa Anzani, Fitri Ari Sunandar Arielda Putri Aditya Armawati Armawati Armawati Armawati, Armawati Asriah Nurdini M. Asriah Nurdini Mardiyyaningsih Asriah Nurdini Mardiyyaningsih Asriah Nurdini Mardiyyaningsih Asriah Nurdini Mardiyyaningsih Atthirilla Binuri Ayu, Febriana Emmytha Bambang Suryobroto Basuki Hardigaluh Bilqis, Khofifah Azmi Budiman, Muhammad Sholid Chaerani Chaerani Dani Abdillah Deska Erasmawanti Desriani Lestari Desta Rotuana Sihombing Desta Rotuana Sihombing Didi Akbar Dita Lindayani Dita, Syifa Ulva Nara Eka Ariyati Eka Mardianti Eka Mardiyanti Eko Sri Wahyuni Eko Sri Wahyuni Eko Sri Wahyuni Eko Widodo Elda Irma Jeanne Joice Kawulur Endah Maulina Eneng Nunuz Rohmatullayaly Entin Daningsih Entin Daningsih Eria Fitriani Eria Fitriani Erna Cristina Pasaribu Fajri, Hayatul Fauzan Daffa Fazry, Nur Fitri Anzani Fitriani, Eria Gelagat, Kristianus Kariman Gita Aprilia Gita Aprilia Gola, Lisa Habibina Habibina Hayatul Fajri Herman, Maria Angelina Bonita Hosniah Hosniah Hosniah, Hosniah Ikhsan Samir Ilma Al-Islami Indriyana, Indriyana Ira Lestari Irma Naura Rifanka Isyatirradhiyah, Isyatirradhiyah Ivan Ivan Junardi Aditya Junardi Junardi Junardi Junardi Junardi Junardi Kartika Prabasari Khusnun Nisa Muftifah Kurniati, Yulia Kusniawati Kusniawati Kusniawati, Kusniawati Kusuma, Apriadi Marki Laili Fiitri Yeni Laili Fitri Yeni Laili Fitri Yeni Lestari, Aini Lisa Gola MAELITA RAMDANI MOEIS Magdalena Magdalena Magdalena Magdalena Mahfut Mardiyanti, Eka Maria Angelina Bonita Herman Maria Angelina Bonita Herman Marinda Sari Sofiyana Mas Akhbar Faturrahman Masriani . Maya Rahmadanty Qairunisa Mega Sintia Mohammad Alfin Rizkiandi Muftifah, Khusnun Nisa Muhammad Reza Muhammad Taufik Riandi Mundiarto, Sigit Murdiyaningsih, Asriah Nurdini Nanda Luthfi Ningsih, Kurnia Ningsih, Kurnia Ningsih Nur Dwi Handarsiputri Nurkaida Winarni Nury Kamelia Oktaviani, Dwi Oktaviani, Rizki Pamane, Yusta Vera Pamela, Pamela Pasaribu, Erna Cristina Pasaribu, Revi Lestari Pristalika, Yunike Putri, Ruqiah Ganda Qairunisa, Maya Rahmadanty Rini Muharini Riska Dawan Tari Robby Firmansyah RR. Ella Evrita Hestiandari Ruqiah Ganda Putri Panjaitan Ruspandi, Ruspandi Salsabila, Alfiya Saripah Nur Asikin Sintia, Mega Sisi Marda Lorensa Sisi Marda Lorensa Siti Fatimah Siti Fatimah Siti Nurhasanah Siti Sahara SITI USWATUN HASANAH Siti Uswatun Hasanah Siti Zubaidah SONY HERU SUMARSONO SONY HERU SUMARSONO Suhara, . Suvani, Monika Susi Syahrina, Syahrina Syamswisna , SYAMSWISNA SYAMSWISNA Syamswisna Syamswisna Syamswisna Syamswisna Syifa Ulva Nara Dita Tenriawaru, Andi Besse Tiara, Marcelini Widya Titin . Titin Hartini Titin Titin Titin Titin Titin Titin Titin Titin Titin Titin Uci Ramadhani Wahyuni, Eko Sri Wahyuni Waskito, Prayogi Widodo Eko widya astuti Winarni, Nurkaida Wiwin Saraswati Wiwin, Mardonius Muliadi Yesi Mila Sari Yogi Dirgari Yokhebed Yokhebed Yuliarsih, Asih Yunike Pristalika Zainiarni, Sri Wahyuni