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All Journal HAYATI Journal of Biosciences Biospecies Biotika: Jurnal Ilmiah Biologi Bioscience Bioeducation Journal Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) AL KAUNIYAH Jurnal Peternakan Indonesia BIOMA Jurnal Pendidikan Biologi Jurnal Penelitian Sains BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi JKTP: Jurnal Kajian Teknologi Pendidikan BIOEDUSCIENCE Jurnal Biologi Tropis ICoTE Proceedings JURNAL PENGABDI Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Bioma : Jurnal Biologi dan Pembelajaran Biologi JPBIO (Jurnal Pendidikan Biologi) Bioedusiana: Jurnal Pendidikan Biologi GERVASI: Jurnal Pengabdian kepada Masyarakat Jurnal Pengabdian Dharma Laksana JURNAL BIOEDUCATION Jurnal Mangifera Edu Reswara: Jurnal Pengabdian Kepada Masyarakat Jurnal Sains dan Edukasi Sains IJPD (International Journal Of Public Devotion) Bioscientist : Jurnal Ilmiah Biologi Integrated Science Education Journal Arwana: Jurnal Ilmiah Program Studi Perairan Jurnal ABM Mengabdi Oryza : Jurnal Pendidikan Biologi TROPICAL GENETICS EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Quagga: Jurnal Pendidikan dan Biologi Tropical Genetics Unnes Science Education Journal Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi Jurnal Perikanan Jurnal Pendidikan MIPA Jurnal Info Kesehatan
WOLLY CANDRAMILA
Universitas Tanjungpura
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Other

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Journal : Tropical Genetics

 1 
Identification of single nucleotide polymorphisms on the D-loop region of mtDNA in Sundanese population Wolly Candramila; Sony Heru Sumarsono; Bambang Suryobroto; Maelita Ramdani Moeis
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Identification of sequence polymorphism on the D-loop region of mtDNA has been done for various purposes, including health and medical treatment. In this research, single nucleotide polymorphisms were identified in the D-loop region of mtDNA of the Sundanese population in western Java. A total of 118 unrelated and healthy Sundanese probands were collected from closed-traditional kampung adat and open communities distributed in 14 cities and regencies in western Java. DNA amplification and direct sequencing of the D-loop region were proceeded using primers L15990 and H409. Multi-alignment was conducted not only intrapopulation but also with D-loop sequence data stored in GenBank for comparison. In this research, we categorized high-frequency SNPs as less effective for identification in population studies because of their presence in other populations outside Indonesia. Meanwhile, lower-frequency SNPs showed typical variants of Sundanese haplotypes. On the other hand, rare or low-frequency SNPs should be re-examined in larger size of samples to have a better understanding of risk factors for many diseases.
Maternal genetic variation of Betawi population Wolly Candramila; Bambang Suryobroto
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Betawi identity was assessed based on the diversity of maternal lines using genetic markers in the D-loop region of mitochondrial DNA. The results of the cleavage by the restriction enzymes DdeI, HaeII, HaeIII, and HinfI showed five patterns of genetic variants which were later named Haplotype I, II, III, IV, and V. Haplotype I had the highest frequency of 75.6%, followed by Haplotype III 11.5%, and Haplotype II 10.3%. Haplotypes IV and V were only found in 1 individual each and were assumed to be individual mutations. The highest haplotype in the Betawi population showed the same restriction enzyme cleavage pattern with all comparison samples from the Sunda and Malay populations. Meanwhile, Haplotype II and III were strongly suspected as the result of the divergence of Haplotype I and can also be found in other ethnic groups in Indonesia. This finding confirms Jakarta as a location for ethnic mixing which then produces a new local culture as Betawi Culture.
Phylogenetic and Genetic Distance Analysis of the Mangrove Worm (Namalycastis) Based on the 18S rRNA Gene Using in Silico Methods Aditya, Arielda Putri; Aditya, Junardi; Candramila, Wolly; Prabasari, Kartika
Tropical Genetics Vol. 5 No. 2 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i2.100

Abstract

Namalycastis is a polychaete worm that inhabits dynamic aquatic environments and exhibits high genetic and morphological variability. This study investigates the phylogeny of the genus Namalycastis through an in silico analysis using the 18S ribosomal RNA (rRNA) gene. This genetic marker is considered ideal as it combines conserved and variable regions, both essential for taxonomic and evolutionary analyses. The objective of this research was to examine the phylogenetic relationships and genetic distances among 12 species of the genus Namalycastis based on their 18S rRNA gene sequences. DNA sequences were obtained from the NCBI database and aligned using Clustal-W in MEGA XI software. Phylogenetic reconstruction was performed using the Neighbor-Joining method with 1000 bootstrap replications, applying the Kimura 2-Parameter (K2P) model. The results revealed that Namalycastis jaya shares a very close evolutionary relationship with Namalycastis abiuma, forming a monophyletic clade that is distinct from Namalycastis hawaiiensis. The genetic distances among N. jaya, N. abiuma, and N. abiuma group sp. indicate a close evolutionary affinity, whereas N. hawaiiensis displays greater genetic divergence from the other two species. Overall, this study demonstrates that the 18S rRNA gene is an effective molecular marker for identifying phylogenetic relationships among Namalycastis species. The findings also highlight the potential of in silico methods in elucidating evolutionary patterns within the genus Namalycastis.
Evaluating Misconceptions in the Representation of Mendelian Genetic Inheritance in High School Biology Textbooks Kusniawati, Kusniawati; Candramila, Wolly; Wahyuni, Eko Sri
Tropical Genetics Vol. 5 No. 2 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i2.107

Abstract

Genetics learning is prone to misconceptions due to complex concepts, abstract reasoning, and inconsistencies in textbook presentations, which can hinder students’ understanding of inheritance patterns such as Mendelian genetics. This study analyzes the potential misconceptions in high school biology textbooks regarding Mendel’s Laws and dominance–recessive inheritance using Dikmenli et al.’s categorization, aiming to provide references for teachers in selecting and supplementing instructional materials. This is a descriptive qualitative research using content analysis method. Four textbooks, selected through a survey of biology teachers in Pontianak City, were analyzed by comparing key concepts in the textbooks with scientifically accurate explanations from authoritative references. The findings were systematically examined, categorized, and validated through member-checking to ensure accuracy, highlighting the most common types of misconceptions in the textbooks. The analysis revealed variations in the coverage and accuracy of 13 key concepts related to Mendel’s Laws and dominance–recessive inheritance, with Books A–C containing several potential misconceptions while Book D showed none. The most frequent types of misconceptions identified were misidentifications, followed by oversimplifications and overgeneralizations, often involving definitions of gene, genotype, phenotype, and independent assortment. These findings highlight the importance of accurate textbook content and suggest that educators must critically evaluate and clarify textbook explanations to prevent persistent misunderstandings in students’ conceptual understanding of genetics.
Co-Authors Ade Mirza Aditya, Arielda Putri Aditya, Junardi Adrianto, Oktavianus Misro Afandi Afandi Afriany, Messy Aini Lestari Akbar, Didi Alda, Alda Aldiansyah, Rifki Andi Besse Tenriawaru Andri Maulidi Anisyah Yuniarti Anzani, Fitri Armawati Armawati Armawati Armawati, Armawati Asriah Nurdini M. Ayu, Febriana Emmytha Bambang Suryobroto Basuki Hardigaluh Bilqis, Khofifah Azmi Budiman, Muhammad Sholid Chaerani Chaerani Dani Abdillah Dani Abdillah Deska Erasmawanti Desta Rotuana Sihombing Desta Rotuana Sihombing Didi Akbar Dita Lindayani Dita, Syifa Ulva Nara Eka Ariyati Eka Mardianti Eka Mardiyanti Eko Sri Wahyuni Eko Widodo Elda Irma Jeanne Joice Kawulur Endah Maulina Eneng Nunuz Rohmatullayaly Entin Daningsih Fajri, Hayatul Fazry, Nur Fitri Anzani Fitriani, Eria Gelagat, Kristianus Kariman Gita Aprilia Gita Aprilia Gola, Lisa Habibina Habibina Hayatul Fajri Herman, Maria Angelina Bonita Hosniah Hosniah Hosniah, Hosniah Ilma Al-Islami Indriyana, Indriyana Ira Lestari Irma Naura Rifanka Isyatirradhiyah, Isyatirradhiyah Junardi Junardi Junardi Junardi Junardi Junardi Kurniati, Yulia Kusniawati, Kusniawati Kusuma, Apriadi Marki Laili Fiitri Yeni Laili Fitri Yeni Lestari, Aini Lestari, Desriani Lisa Gola MAELITA RAMDANI MOEIS MAELITA RAMDANI MOEIS Magdalena Magdalena Magdalena Magdalena Mahfut Mardiyanti, Eka Maria Angelina Bonita Herman Maria Angelina Bonita Herman Marinda Sari Sofiyana Masriani . Mega Sintia Muftifah, Khusnun Nisa Muhammad Reza Muhammad Taufik Riandi Mundiarto, Sigit Murdiyaningsih, Asriah Nurdini Ningsih, Kurnia Ningsih, Kurnia Ningsih Nur Dwi Handarsiputri Nurkaida Winarni Nury Kamelia Oktaviani, Dwi Pamane, Yusta Vera Pamela, Pamela Pasaribu, Erna Cristina Pasaribu, Revi Lestari Prabasari, Kartika Pristalika, Yunike Putri, Ruqiah Ganda Qairunisa, Maya Rahmadanty Rini Muharini Riska Dawan Tari Rizki Oktaviani Oktaviani Robby Firmansyah RR. Ella Evrita Hestiandari Ruqiah Ganda Putri Panjaitan Ruspandi, Ruspandi Salsabila, Alfiya Sintia, Mega Sisi Marda Lorensa Sisi Marda Lorensa Siti Fatimah Siti Fatimah Siti Nurhasanah Siti Sahara Siti Zubaidah SONY HERU SUMARSONO SONY HERU SUMARSONO Suhara, . Sunandar, Ari Suvani, Monika Susi Syahrina, Syahrina Syamswisna , Syifa Ulva Nara Dita Tenriawaru, Andi Besse Tiara, Marcelini Widya Titin . Titin Hartini Titin Titin Titin Titin Titin Titin Titin Titin Uci Ramadhani Wahyuni, Eko Sri Wahyuni Widodo Eko widya astuti Winarni, Nurkaida Wiwin, Mardonius Muliadi Yesi Mila Sari Yogi Dirgari Yokhebed Yokhebed Yuliarsih, Asih Yunike Pristalika Zainiarni, Sri Wahyuni