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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Ilmu Ternak JURNAL ILMU DAN TEKNOLOGI PETERNAKAN JURNAL SAINS PERTANIAN EQUATOR Jurnal Ekonomi, Bisnis dan Kewirausahaan (JEBIK) Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Sains Peternakan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Teknologi Pertanian Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Indonesian Journal of Chemistry Jurnal Teknologi Pertanian (Agricultural Technology Journal) JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Medik Veteriner Jurnal Ilmiah Fillia Cendekia Jurnal Nutrisi Ternak Tropis (JNT) Abdimasku : Jurnal Pengabdian Masyarakat Tarjih Tropical Livestock Journal Aves : Jurnal Ilmu Peternakan
Yuli Arif Tribudi
Fakultas Pertanian, Universitas Tanjungpura, Pontianak
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Social Sciences Veterinary Other

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CHARACTERISTICS OF THE BODY SIZE OF THE SENDURO GOAT WHICH IS ORIGIN FROM SENDURO, LUMAJANG, EAST JAVA Guruh Prasetyo; Tri Eko Susilorini; Wike Andre Septian; Yuli Arif Tribudi; Ahmad Zarkasi Efendi; Nur Hidayah; Heri Damayanti; Suyadi Suyadi
Jurnal Veteriner Vol 24 No 2 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.2.230

Abstract

Germplasm diversity is a valuable source of genetic material whose existence needs to be protected and preserved. One of the original Indonesian goat germplasms is Senduro goat, which has been established by the Decree of the Ministry of Agriculture Republic of Indonesia No. 1055/Kpts/ SR.120/10/2014 and originates from Senduro District, Lumajang Regency, East Java. The purpose of the study was to analyze the phenotypic traits of Senduro goats based on their quantitative traits. Phenotype observations include body length, chest circumference, shoulder height, ear length, and rewos hair length and correlate each phenotype trait. The materials used in this study were 14 and 25 head of adult male and female Senduro goats, respectively. Senduro goats that were observed in the condition of incisor the thirdth (I3 ) were replaced by permanent teeth, it mean the Senduro goats aged 2-4 years. The morphometric data obtained were calculated as mean, standard deviation, and correlation between morphometric measures using the Genstat 14.2 program and then analyzed descriptively. The results of this study showed that Senduro goats had a body weight of 24.56 ± 5.48 kg (male) and 26.84 ± 6.50 kg (female), a body length of 63.89 ± 4.76 cm (male) and 63.32 ± 6.29 cm (female), a chest circumference of 61.67 ± 4.36 kg (male) and 66, 48 ± 6.56 cm (female), a shoulder height of 66.33 ± 4.53 cm (male) and 64.71 ± 5.76 cm (female), ear length of 31.22 ± 2.17 cm (male) and 28.13 ± 2.74 cm (female), and length of rewos/surai hair of 17.56 ± 1.67 cm (male) and 14.58 ± 2.39 cm (female) Morphometric measures in Senduro goats are positively correlated with each other. There is a highly significant correlation between chest circumference, body length, and height and body weight in Senduro goats.
Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples Azizah, Nurul; Kostaman, Tatan; Tribudi, Yuli Arif; Kusumaningrum, Diana Andrianita; Suyatno, Suyatno; Muttaqin, Zultinur; Koswara, Eko; Gunawan, Muhammad
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.292-299

Abstract

The optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompassed two treatment groups, each iterated six replication. Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. Cryopreservation included the controlled freezing of blood and purified PGCs samples in Nalgene cryogenic vials with the incorporation of banker cells. Following thawing, statistical analyses revealed no significant variance in the mean PGCs count between purified PGCs and blood samples (p > 0.05). The percentages of PGCs mortality and the recovery rate demonstrated statistical significance (p < 0.05). Further investigations indicated that cryopreserving blood samples significantly increased the recovery rate of PGCs while decreasing post-thaw mortality. Morphology of PGCs from blood cryopreservation showed similar morphology to PGCs in general. The potential application of cell-based cryo-banking will contribute to the preservation of poultry genetic diversity for sustainability and adaptation to future poultry demands.
Komponen Bioaktif pada Tanaman Centrocema Pubescens dan Potensinya sebagai Pakan Ternak Peni Wahyu Prihandini; Henny Leondro; Yuli Arif Tribudi; Aju Tjatur Nugroho Krisnaningsih; Dimas Pratidina Puriastuti Hadiani; Dewi Khosiya Robba; Dyah Tuwi Ramsiati; Mochammad Chanafi; Wahyuni Indah Wulansari
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 1 (2024): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.01.6

Abstract

Centrosema pubescens adalah leguminosa merambat yang dimanfaatkan sebagai pakan ternak karena kandungan proteinnya yang tinggi. Namun, penelitian seputar tanaman Centrosema pubescens masih sangat minim, terutama dalam bidang identifikasi senyawa bioaktif. Penelitian ini bertujuan untuk mengidentifikasi senyawa bioaktif yang terkandung dalam ekstrak Centrosema pubescens. Bagian daun, batang dan bunga tanaman Centrosema pubescent diekstraksi menggunakan pelarut etanol 70%, dipekatkan menggunakan rotary evaporator, kemudian dianalisis menggunakan perangkat LC-MS. Hasil analisis LC-MS menunjukkan keberadaan 86 senyawa yang diketahui. Kromatogram pada tanaman Centrosema pubescens terdapat satu puncak tertinggi dengan komposisi 3,490% yaitu senyawa prodephinidin C2 (golongan senyawa flavonoid). Lebih lanjut, senyawa golongan flavonoid glikosida seperti rutin, naringin, dan kaempferol juga terindentifikasi pada legum ini. Untuk senyawa yang teridentifikasi yaitu 20-hydroxyecdysone. Berdasarkan banyaknya senyawa bioaktif pada Centrosema pubescens, maka dapat direkomendasikan sebagai fitobiotik pada pakan ternak
Vitamin E Supplementation to Quail (Coturnix-coturnix Japonica) Treated by Heat Stress: Effects on Blood Hematology Parameters Astuti, Farida Kusuma; Rinanti, Rosyida Fajri; Nurul, Mohamad; Tribudi, Yuli Arif
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 2 (2024): JNT Jurnal Nutrisi Ternak Tropis September
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.02.7

Abstract

Heat stress is a major problem in poultry farming.  Environmental temperature and humidity greatly in fluence the performance of poultry, especially quails raised in tropical environments. Vitamin E is a fat-soluble vitamin that has an antioxidant effect. This study aimed to evaluate the effect of vitamin E supplementation on the blood profile of quails kept under heat stress. This study used 4 treatments of T0 as Control (Commercial Feed + Heat Stress 35 oC); T1 (Control + 100 mg of vitamin E/kg); T2 (Control + 150 mg of vitamin E/kg) and T3 (Control + 200 mg of vitamin E/kg). Observed parameters of leukocytes, heterophylls, lymphocytes and monocytes. The data was analyzed by Analysis of Variant (ANOVA) if there were differences between treatments, followed by the Duncan test. The observation results showed that the addition of vitamin E could increase the number of leukocytes, lymphocytes, monocytes and reduce the number of heterophytes and the H/L ratio of quail blood that experienced heat stroke. Based on the blood profile, the addition of vitamin E as much as 200 mg/kg in feed is able to reduce heat stress due to heat stress in laying quails.
The Conservation on Indonesian Native Chicken Biodiversity Through Primordial Germ Cells Cryopreservation Tribudi, Yuli Arif; Kostaman, Tatan
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.682 KB) | DOI: 10.33772/jitro.v9i1.20400

Abstract

ABSTRACT Primordial germ cells (PGCs) can be cryopreserved and used as a potential tool for the preservation of poultry biodiversity. By transplanting donor PGCs into recipient embryos to produce chimera germline, PGCs can migrate to developing gonads where germ cells are produced, thus enabling reproduction of offspring derived from donor PGCs via mating of chimera germline. Collection of PGCs from the circulation of chickens. Fertile eggs were incubated in a portable incubator for 2.5 days. Purification of circulating PGCs with nycodenz. Then the PGCs were frozen using the 5% DMSO cryoprotectant and to determine the success of cryopreservation, the donor PGCs were transplanted into the recipient embryos. The number of circulating PGCs was 50.58 ± 5.79 cells per embryo, the percentage of PGCs viability after thawing was 80.18 ± 1.25%. From the results of the PGCs transplant by looking at the migration of donor PGCs in recipient embryos with PKH-16 staining, it is clear. In conclusion, PGCs cryopreservation in this study can generally support the survival of good chickens and it is hoped that this PGCs conservation protocol can be used for other native chickens.Keywords: PGC, cryopreservation, chicken, conservation
Clitoria ternatea Increases Milk Production in Dairy Cows by Inhibiting Dopamine Receptor D2: A Computational Study Leondro, Henny; Hadiani, Dimas Pratidina Puriastuti; Krisnaningsih, Aju Tjatur Nugroho; Lestari, Oke Anandika; Wahyudi, Didik; Gusmalawati, Dwi; Tribudi, Yuli Arif; Prihandini, Peni Wahyu
Indonesian Journal of Chemistry Vol 24, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.92920

Abstract

Dairy cow's milk is a primary commodity in various countries and increasing milk production in dairy cows is crucial. Clitoria ternatea has the potential to enhance milk production in dairy cows. This research aims to analyze C. ternatea's ability to induce milk production in dairy cows by targeting the DRD2 protein. The compounds within C. ternatea were screened for drug-likeness, toxicity, physicochemical properties, and membrane permeability parameters. The DRD2 protein in dairy cattle was modeled using homology modeling. The interaction stability between C. ternatea compounds and DRD2 was analyzed through molecular docking and dynamic using AutoDock Vina and Webgro. The study results revealed that among the 18 compounds, 5 passed the drug-likeness screening: citronellal, alpha-terpinolene, 15-methyxypaysine, allyl-crotyl-zinc, and 9,12-octadecadiynoic. These 5 compounds exhibited low toxicity and demonstrated easy penetration of lipid membranes. Molecular docking results indicated that citronellal and alpha-terpinolene had the lowest binding energy values and were bound to the inhibitor's side. Molecular dynamic simulations also confirmed the stability of the interaction between citronellal and alpha-terpinolene with DRD2. In conclusion, this research suggests that C. ternatea can potentially increase milk production in dairy cows by inhibiting the DRD2 protein, primarily through citronellal and alpha-terpinolene.
First Screening of Heat Shock 70kDa Protein 1A (HSPA1A) Gene Polymorphisms in Indonesian Local Sheep Sari, Apriliana Putri Zahara Nafsina Luvita; Ibrahim, Alek; Tribudi, Yuli Arif; Maulana, Yasin Pradana
Tarjih Tropical Livestock Journal Vol. 4 No. 2 (2024): Tarjih-Tropical Livestock Journal
Publisher : Program Studi Peternakan Fakultas Sains dan Teknologi Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/trolija.v4i2.862

Abstract

Heat shock proteins (HSPs) are a collection of proteins that promote thermotolerance in cells and protect against environmental stress. This study was a preliminary analysis to identify the nucleotide variant within the partial heat shock 70kDa protein 1A (HSPA1A) gene in several Indonesian sheep breeds. The blood samples were collected from five breeds of Indonesian sheep (Sapudi, Batur, Sakub, Wonosobo, and Garut) to be analyzed and amplified using a specific primer. The sequences were aligned using Clustal Omega to identify the single nucleotide polymorphism (SNP). An ExPasy program was used to detect the amino acid changes. The genotyping was done by direct examination of the peak of the electropherogram. As a result, based on the sequence alignment and direct observation of the electropherograms, three variants were found: SNP c.286A>G, c.503A>G, and c.733A>G. All SNPs were categorized as synonymous mutations since they did not alter the amino acid. The genotyping of samples showed two types of genotypes (AG and GG) from each SNP. In conclusion, our study suggests exploring other regions of the HSPA1A gene to find candidate mutations for further genetic analysis.
Genetic External Characteristics of Tukong: Indonesian Rumpless Indigenous Chicken Tribudi, Yuli Arif; Nurgiartiningsih, Veronica Margaretha Ani; Ulfah, Maria; Sari, Aprilianna Putri Zahara Nafsina Luvita; Hariyono, Dwi Nur Happy; Sitaresmi, Pradita Iustitia; Crooijmans, Richard PMA; Natsir, Muhammad Halim; Gusmalawati, Dwi; Ibrahim, Alek
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1147-1156

Abstract

Tukong chicken, also known as rumpless chicken due to the absence of a tailbone, is one of the indigenous chicken breeds in Indonesia currently at risk of extinction. Due to its scarcity, genetic information on the Tukong chicken remains limited, and its potential has not been fully explored. Therefore, this study aimed to determine the genetic external characteristics of the Tukong chickens. Data were collected from 205 chickens (52 males; 153 females) raised in Landak and Bengkayang Regency, West Kalimantan. The varieties are based on feather color, plumage color, feather pattern, feather flicker, comb shape, and shank color. The data were used to analyze the rate of the original gene, the frequency of autosomal genes, sex-linked genes, feather pattern, introgression rate, gene frequency, and expected heterozygosity. The majority of the Tukong chickens had colored feathers (88.78%), columbian feather type (47.80%), plain feather pattern (61.46%), silver feather flicker (54.63%), yellow-white shank (62.44%), and pea comb (77.07%), which were controlled by ii, ee, bb, SS, IdId, and P_ genes, respectively. The introgression rate from exotic breeds into Tukong chicken is 53.2%, with the original gene at 46.8%, while the average expected heterozygosity was 0.416±0.025, indicating a high level of genetic diversity of the traits. This observed variation in qualitative morphological characteristics might benefit both the conservation and utilization of the Tukong chicken.
Identification of Single-Nucleotide Polymorphism, Linkage Disequilibrium, and Restriction Enzyme Analysis of the Growth Hormone Gene in Kampung Super Chicken Sari, Apriliana Putri Zahara Nafsina Luvita; Maharani, Dyah; Febrianto, Firman; Maulana, Yasin Pradana; Tribudi, Yuli Arif
Tarjih Tropical Livestock Journal Vol. 5 No. 1 (2025): Tarjih Tropical Livestock Journal
Publisher : Program Studi Peternakan Fakultas Sains dan Teknologi Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/trolija.v5i1.936

Abstract

This study aimed to investigate single-nucleotide polymorphisms (SNPs) and haplotype diversity within the growth hormone (GH) gene of Kampung Super chickens, a hybrid breed derived from KUB males and commercial layer hens. In this study, thirty-eight blood samples of Kampung Super chicken were collected and analyzed. A total of 11 SNPs were identified within intronic regions of the GH gene. Despite their non-coding location, these variants may influence gene regulation through mechanisms such as alternative splicing, mRNA transport, or chromatin accessibility. Linkage disequilibrium (LD) analysis revealed several strong associations among SNP pairs, indicating the presence of conserved haplotype blocks potentially under stabilizing selection. In silico restriction enzyme analysis showed that five SNPs displayed three genotypic classes, and four of them could be effectively genotyped using PCR-RFLP, while one SNP (g.3018G/A) lacked suitable restriction sites. The SNP g.3094C/T exhibited high diagnostic potential using multiple enzymes (StuI, MspI, XmaI), while g.3129A/T was distinguishable using TaqI, although the adjacent SNP overlap limited interpretability. Two SNPs (g.3261C/T and g.3268G/A) were digestible by enzymes with multiple cut sites, complicating gel-based resolution. These findings provide a foundation for future functional and association studies and support the use of GH gene polymorphisms in marker-assisted selection strategies for growth-related traits in Kampung Super chicken.
Effect of Pineapple Peel Probiotics in Drinking Water on The Production Performance and Egg Quality of Quail Lestari, Retno Budi; Permadi, Edy; Andri, Andri; Tribudi, Yuli Arif; Mustaqim, Zainul
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 2 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i2.p255-267

Abstract

This study aimed to evaluate the effects of adding pineapple peel probiotics to drinking water on quail productivity and egg quality. The experiment used 240 Peksi strain quails, aged 12 weeks, and pineapple peel probiotics containing Lactobacillus casei. A completely randomized design was applied, with six treatment groups receiving varying concentrations of pineapple peel probiotics in drinking water: 0% (P0), 1%/liter (P1), 2%/liter (P2), 3%/liter (P3), 4%/liter (P4), and 5%/liter (P5). The observed parameters included feed consumption, hen-day production (HDP), egg weight, feed conversion ratio, and the fat and cholesterol content of the eggs. Data were analyzed using ANOVA, followed by Duncan's test for post-hoc comparisons where significant differences were observed. The addition of pineapple peel probiotics in drinking water did not significantly affect (P>0.05) feed consumption, daily egg production, egg weight, feed conversion ratio, or the fat and cholesterol content of the eggs. However, supplementation at pineapple peel probiotics in drinking water to can daily egg production in quails.
Co-Authors Achmad Munir Agustina Widyaworo Kunharjati Ahmad Tohardi Ahmad Tohardi Ahmad Zarkasi Efendi Aju Tjatur Nugroho Krisnaningsih Alek Ibrahim Ali Mahmud Andri Andri Andri Andri Andri Andri Angga Permadi Karpriana Ardi Libra Ryadi Arif Hidayatullah Arina Nurfianti Ayu Tri Agustin Bonifasius Eri Budi Utomo Crooijmans, Richard PMA Dani Susanto Deny Sulistyowati Dewi Khosiya Robba Dian Eka Setyaningtyas Diana Andrianita Kusumaningrum, Diana Andrianita Didik Wahyudi Dwi Gusmalawati Dyah Maharani Dyah Tuwi Ramsiati Farida Kusuma Astuti Farida Kusuma Astuti, Farida Kusuma Febrianto, Firman Freselia Ria Pratiwi Guruh Prasetyo Hadiani, Dimas Pratidina Puriastuti Handika Rifki W.P Hariyono, Dwi Nur Happy Heri Damayanti Irsa Sylviandari Josua Parulian Hutajulu Koswara, Eko Leondro, Henny Maria Ulfah Maulana, Yasin Pradana Mochammad Chanafi Muhammad Gunawan Muhammad Halim Natsir Murianto, Benni Musa Alfius Mustaqim, Zainul Nining Haryuni Nisa’us Sholikah Novianti Novianti Nur Hidayah Nurgiartiningsih, V. M. A. Nurgiartiningsih, Veronica Margaretha Ani Nuria Fa&#039;ul Jannah NURUL AZIZAH Nurul, Mohamad Nurwahid Nurwahid Oke Anandika Lestari Peni W. Prihandini Peni Wahyu Prihandini Peni Wahyu Prihandini Permadi, Edy Prihandini, Peni Wahyu Puguh Surjowardojo Rakhmad Perkasa Harahap Retno Budi Lestari Retno Budi Lestari Rinanti, Rosyida Fajri Ristito Widodo Ristyawan, Mochammad Ridwan Sari, Apriliana Putri Zahara Nafsina Luvita Sari, Aprilianna Putri Zahara Nafsina Luvita Sitaresmi, Pradita Iustitia Sri Susilowati Suyadi Suyadi Suyatno Suyatno Tatan Kostaman Tri Eko Susilorini Utin Yulia Via Anggi Agustin Vindo Lesmana Wahidin Yoga Febriyanto Wahyuni Indah Wulansari Wike andre Septian Woro Busono, Woro Yashinta Salsabila zakiya tulyaqin Zultinur Muttaqin