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Komponen Bioaktif pada Tanaman Centrocema Pubescens dan Potensinya sebagai Pakan Ternak Peni Wahyu Prihandini; Henny Leondro; Yuli Arif Tribudi; Aju Tjatur Nugroho Krisnaningsih; Dimas Pratidina Puriastuti Hadiani; Dewi Khosiya Robba; Dyah Tuwi Ramsiati; Mochammad Chanafi; Wahyuni Indah Wulansari
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 1 (2024): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.01.6

Abstract

Centrosema pubescens adalah leguminosa merambat yang dimanfaatkan sebagai pakan ternak karena kandungan proteinnya yang tinggi. Namun, penelitian seputar tanaman Centrosema pubescens masih sangat minim, terutama dalam bidang identifikasi senyawa bioaktif. Penelitian ini bertujuan untuk mengidentifikasi senyawa bioaktif yang terkandung dalam ekstrak Centrosema pubescens. Bagian daun, batang dan bunga tanaman Centrosema pubescent diekstraksi menggunakan pelarut etanol 70%, dipekatkan menggunakan rotary evaporator, kemudian dianalisis menggunakan perangkat LC-MS. Hasil analisis LC-MS menunjukkan keberadaan 86 senyawa yang diketahui. Kromatogram pada tanaman Centrosema pubescens terdapat satu puncak tertinggi dengan komposisi 3,490% yaitu senyawa prodephinidin C2 (golongan senyawa flavonoid). Lebih lanjut, senyawa golongan flavonoid glikosida seperti rutin, naringin, dan kaempferol juga terindentifikasi pada legum ini. Untuk senyawa yang teridentifikasi yaitu 20-hydroxyecdysone. Berdasarkan banyaknya senyawa bioaktif pada Centrosema pubescens, maka dapat direkomendasikan sebagai fitobiotik pada pakan ternak
Vitamin E Supplementation to Quail (Coturnix-coturnix Japonica) Treated by Heat Stress: Effects on Blood Hematology Parameters Astuti, Farida Kusuma; Rinanti, Rosyida Fajri; Nurul, Mohamad; Tribudi, Yuli Arif
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 2 (2024): JNT Jurnal Nutrisi Ternak Tropis September
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.02.7

Abstract

Heat stress is a major problem in poultry farming.  Environmental temperature and humidity greatly in fluence the performance of poultry, especially quails raised in tropical environments. Vitamin E is a fat-soluble vitamin that has an antioxidant effect. This study aimed to evaluate the effect of vitamin E supplementation on the blood profile of quails kept under heat stress. This study used 4 treatments of T0 as Control (Commercial Feed + Heat Stress 35 oC); T1 (Control + 100 mg of vitamin E/kg); T2 (Control + 150 mg of vitamin E/kg) and T3 (Control + 200 mg of vitamin E/kg). Observed parameters of leukocytes, heterophylls, lymphocytes and monocytes. The data was analyzed by Analysis of Variant (ANOVA) if there were differences between treatments, followed by the Duncan test. The observation results showed that the addition of vitamin E could increase the number of leukocytes, lymphocytes, monocytes and reduce the number of heterophytes and the H/L ratio of quail blood that experienced heat stroke. Based on the blood profile, the addition of vitamin E as much as 200 mg/kg in feed is able to reduce heat stress due to heat stress in laying quails.
The Conservation on Indonesian Native Chicken Biodiversity Through Primordial Germ Cells Cryopreservation Tribudi, Yuli Arif; Kostaman, Tatan
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.682 KB) | DOI: 10.33772/jitro.v9i1.20400

Abstract

ABSTRACT Primordial germ cells (PGCs) can be cryopreserved and used as a potential tool for the preservation of poultry biodiversity. By transplanting donor PGCs into recipient embryos to produce chimera germline, PGCs can migrate to developing gonads where germ cells are produced, thus enabling reproduction of offspring derived from donor PGCs via mating of chimera germline. Collection of PGCs from the circulation of chickens. Fertile eggs were incubated in a portable incubator for 2.5 days. Purification of circulating PGCs with nycodenz. Then the PGCs were frozen using the 5% DMSO cryoprotectant and to determine the success of cryopreservation, the donor PGCs were transplanted into the recipient embryos. The number of circulating PGCs was 50.58 ± 5.79 cells per embryo, the percentage of PGCs viability after thawing was 80.18 ± 1.25%. From the results of the PGCs transplant by looking at the migration of donor PGCs in recipient embryos with PKH-16 staining, it is clear. In conclusion, PGCs cryopreservation in this study can generally support the survival of good chickens and it is hoped that this PGCs conservation protocol can be used for other native chickens.Keywords: PGC, cryopreservation, chicken, conservation
Clitoria ternatea Increases Milk Production in Dairy Cows by Inhibiting Dopamine Receptor D2: A Computational Study Leondro, Henny; Hadiani, Dimas Pratidina Puriastuti; Krisnaningsih, Aju Tjatur Nugroho; Lestari, Oke Anandika; Wahyudi, Didik; Gusmalawati, Dwi; Tribudi, Yuli Arif; Prihandini, Peni Wahyu
Indonesian Journal of Chemistry Vol 24, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.92920

Abstract

Dairy cow's milk is a primary commodity in various countries and increasing milk production in dairy cows is crucial. Clitoria ternatea has the potential to enhance milk production in dairy cows. This research aims to analyze C. ternatea's ability to induce milk production in dairy cows by targeting the DRD2 protein. The compounds within C. ternatea were screened for drug-likeness, toxicity, physicochemical properties, and membrane permeability parameters. The DRD2 protein in dairy cattle was modeled using homology modeling. The interaction stability between C. ternatea compounds and DRD2 was analyzed through molecular docking and dynamic using AutoDock Vina and Webgro. The study results revealed that among the 18 compounds, 5 passed the drug-likeness screening: citronellal, alpha-terpinolene, 15-methyxypaysine, allyl-crotyl-zinc, and 9,12-octadecadiynoic. These 5 compounds exhibited low toxicity and demonstrated easy penetration of lipid membranes. Molecular docking results indicated that citronellal and alpha-terpinolene had the lowest binding energy values and were bound to the inhibitor's side. Molecular dynamic simulations also confirmed the stability of the interaction between citronellal and alpha-terpinolene with DRD2. In conclusion, this research suggests that C. ternatea can potentially increase milk production in dairy cows by inhibiting the DRD2 protein, primarily through citronellal and alpha-terpinolene.
First Screening of Heat Shock 70kDa Protein 1A (HSPA1A) Gene Polymorphisms in Indonesian Local Sheep Sari, Apriliana Putri Zahara Nafsina Luvita; Ibrahim, Alek; Tribudi, Yuli Arif; Maulana, Yasin Pradana
Tarjih Tropical Livestock Journal Vol. 4 No. 2 (2024): Tarjih-Tropical Livestock Journal
Publisher : Program Studi Peternakan Fakultas Sains dan Teknologi Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/trolija.v4i2.862

Abstract

Heat shock proteins (HSPs) are a collection of proteins that promote thermotolerance in cells and protect against environmental stress. This study was a preliminary analysis to identify the nucleotide variant within the partial heat shock 70kDa protein 1A (HSPA1A) gene in several Indonesian sheep breeds. The blood samples were collected from five breeds of Indonesian sheep (Sapudi, Batur, Sakub, Wonosobo, and Garut) to be analyzed and amplified using a specific primer. The sequences were aligned using Clustal Omega to identify the single nucleotide polymorphism (SNP). An ExPasy program was used to detect the amino acid changes. The genotyping was done by direct examination of the peak of the electropherogram. As a result, based on the sequence alignment and direct observation of the electropherograms, three variants were found: SNP c.286A>G, c.503A>G, and c.733A>G. All SNPs were categorized as synonymous mutations since they did not alter the amino acid. The genotyping of samples showed two types of genotypes (AG and GG) from each SNP. In conclusion, our study suggests exploring other regions of the HSPA1A gene to find candidate mutations for further genetic analysis.
Genetic External Characteristics of Tukong: Indonesian Rumpless Indigenous Chicken Tribudi, Yuli Arif; Nurgiartiningsih, Veronica Margaretha Ani; Ulfah, Maria; Sari, Aprilianna Putri Zahara Nafsina Luvita; Hariyono, Dwi Nur Happy; Sitaresmi, Pradita Iustitia; Crooijmans, Richard PMA; Natsir, Muhammad Halim; Gusmalawati, Dwi; Ibrahim, Alek
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1147-1156

Abstract

Tukong chicken, also known as rumpless chicken due to the absence of a tailbone, is one of the indigenous chicken breeds in Indonesia currently at risk of extinction. Due to its scarcity, genetic information on the Tukong chicken remains limited, and its potential has not been fully explored. Therefore, this study aimed to determine the genetic external characteristics of the Tukong chickens. Data were collected from 205 chickens (52 males; 153 females) raised in Landak and Bengkayang Regency, West Kalimantan. The varieties are based on feather color, plumage color, feather pattern, feather flicker, comb shape, and shank color. The data were used to analyze the rate of the original gene, the frequency of autosomal genes, sex-linked genes, feather pattern, introgression rate, gene frequency, and expected heterozygosity. The majority of the Tukong chickens had colored feathers (88.78%), columbian feather type (47.80%), plain feather pattern (61.46%), silver feather flicker (54.63%), yellow-white shank (62.44%), and pea comb (77.07%), which were controlled by ii, ee, bb, SS, IdId, and P_ genes, respectively. The introgression rate from exotic breeds into Tukong chicken is 53.2%, with the original gene at 46.8%, while the average expected heterozygosity was 0.416±0.025, indicating a high level of genetic diversity of the traits. This observed variation in qualitative morphological characteristics might benefit both the conservation and utilization of the Tukong chicken.
Identification of Single-Nucleotide Polymorphism, Linkage Disequilibrium, and Restriction Enzyme Analysis of the Growth Hormone Gene in Kampung Super Chicken Sari, Apriliana Putri Zahara Nafsina Luvita; Maharani, Dyah; Febrianto, Firman; Maulana, Yasin Pradana; Tribudi, Yuli Arif
Tarjih Tropical Livestock Journal Vol. 5 No. 1 (2025): Tarjih Tropical Livestock Journal
Publisher : Program Studi Peternakan Fakultas Sains dan Teknologi Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/trolija.v5i1.936

Abstract

This study aimed to investigate single-nucleotide polymorphisms (SNPs) and haplotype diversity within the growth hormone (GH) gene of Kampung Super chickens, a hybrid breed derived from KUB males and commercial layer hens. In this study, thirty-eight blood samples of Kampung Super chicken were collected and analyzed. A total of 11 SNPs were identified within intronic regions of the GH gene. Despite their non-coding location, these variants may influence gene regulation through mechanisms such as alternative splicing, mRNA transport, or chromatin accessibility. Linkage disequilibrium (LD) analysis revealed several strong associations among SNP pairs, indicating the presence of conserved haplotype blocks potentially under stabilizing selection. In silico restriction enzyme analysis showed that five SNPs displayed three genotypic classes, and four of them could be effectively genotyped using PCR-RFLP, while one SNP (g.3018G/A) lacked suitable restriction sites. The SNP g.3094C/T exhibited high diagnostic potential using multiple enzymes (StuI, MspI, XmaI), while g.3129A/T was distinguishable using TaqI, although the adjacent SNP overlap limited interpretability. Two SNPs (g.3261C/T and g.3268G/A) were digestible by enzymes with multiple cut sites, complicating gel-based resolution. These findings provide a foundation for future functional and association studies and support the use of GH gene polymorphisms in marker-assisted selection strategies for growth-related traits in Kampung Super chicken.
Effect of Pineapple Peel Probiotics in Drinking Water on The Production Performance and Egg Quality of Quail Lestari, Retno Budi; Permadi, Edy; Andri, Andri; Tribudi, Yuli Arif; Mustaqim, Zainul
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 2 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i2.p255-267

Abstract

This study aimed to evaluate the effects of adding pineapple peel probiotics to drinking water on quail productivity and egg quality. The experiment used 240 Peksi strain quails, aged 12 weeks, and pineapple peel probiotics containing Lactobacillus casei. A completely randomized design was applied, with six treatment groups receiving varying concentrations of pineapple peel probiotics in drinking water: 0% (P0), 1%/liter (P1), 2%/liter (P2), 3%/liter (P3), 4%/liter (P4), and 5%/liter (P5). The observed parameters included feed consumption, hen-day production (HDP), egg weight, feed conversion ratio, and the fat and cholesterol content of the eggs. Data were analyzed using ANOVA, followed by Duncan's test for post-hoc comparisons where significant differences were observed. The addition of pineapple peel probiotics in drinking water did not significantly affect (P>0.05) feed consumption, daily egg production, egg weight, feed conversion ratio, or the fat and cholesterol content of the eggs. However, supplementation at pineapple peel probiotics in drinking water to can daily egg production in quails.
PENINGKATAN KAPASITAS PETERNAK ENTOK MELALUI TEKNOLOGI BREEDING DAN PEMASARAN DIGITAL Yuli Arif Tribudi; Andri Andri; Dwi Gusmalawati; Aprilianna Putri Zahara Nafsina Luvita Sari
Jurnal Abdi Negeriku Vol 5, No 1 (2026): Jurnal Abdi Negeriku
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/jan.v5i1.84956

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan peternak entok terkait manajemen breeding dan pemasaran digital di Desa Rasau Jaya III, Kabupaten Kubu Raya, Kalimantan Barat. Kegiatan dilaksanakan pada tanggal 11 Mei 2026 dengan melibatkan 15 peternak entok menggunakan pendekatan partisipatif melalui penyuluhan, Focus Group Discussion (FGD), pelatihan, dan pendampingan. Materi yang diberikan meliputi teknik seleksi bibit unggul, manajemen perkawinan, recording ternak, penetasan dan brooding, serta strategi pemasaran digital melalui media sosial dan platform daring. Evaluasi keberhasilan program dilakukan menggunakan metode pre-test dan post-test untuk mengukur peningkatan pemahaman peternak. Hasil kegiatan menunjukkan adanya peningkatan skor pengetahuan pada seluruh aspek pelatihan, yaitu recording ternak dari 59 menjadi 78, pemahaman perkawinan entok dari 73 menjadi 91, dan pemasaran digital dari 62 menjadi 83. Selain itu, peternak mulai mampu menerapkan pencatatan data ternak, memahami teknik reproduksi yang lebih terarah, serta memanfaatkan media digital sebagai sarana promosi dan pemasaran produk. Program ini menunjukkan bahwa edukasi berbasis partisipatif mampu meningkatkan kapasitas teknis dan kemampuan pemasaran peternak entok secara efektif. Kegiatan ini diharapkan dapat mendukung peningkatan produktivitas, pendapatan, dan keberlanjutan usaha peternakan entok skala rakyat di Desa Rasau Jaya III.
Co-Authors Achmad Munir Agustin, Ayu Tri Agustina Widyaworo Kunharjati Ahmad Tohardi Ahmad Tohardi Ahmad Zarkasi Efendi Aju Tjatur Nugroho Krisnaningsih Alek Ibrahim Ali Mahmud Andri Andri Andri Andri Andri Andri Andri andri Aprilianna Putri Zahara Nafsina Luvita Sari Ardi Libra Ryadi Arif Hidayatullah Arina Nurfianti Bonifasius Eri Budi Utomo Crooijmans, Richard PMA Dani Susanto Deny Sulistyowati Dewi Khosiya Robba Dian Eka Setyaningtyas Diana Andrianita Kusumaningrum, Diana Andrianita Didik Wahyudi Dwi Gusmalawati Dyah Maharani Dyah Tuwi Ramsiati Fachry Abda El Rahman Farida Kusuma Astuti Farida Kusuma Astuti, Farida Kusuma Febrianto, Firman Freselia Ria Pratiwi Guruh Prasetyo Hadiani, Dimas Pratidina Puriastuti Handika Rifki W.P Hariyono, Dwi Nur Happy Heri Damayanti Irsa Sylviandari Josua Parulian Hutajulu Karpriana, Angga Permadi Koswara, Eko Leondro, Henny Maria Ulfah Maulana, Yasin Pradana Mochammad Chanafi Muhammad Gunawan Muhammad Halim Natsir Murianto, Benni Musa Alfius Mustaqim, Zainul Nining Haryuni Nisa’us Sholikah Nur Hidayah Nurgiartiningsih, V. M. A. Nurgiartiningsih, Veronica Margaretha Ani Nuria Fa'ul Jannah NURUL AZIZAH Nurul, Mohamad Nurwahid Nurwahid Oke Anandika Lestari Peni W. Prihandini Peni Wahyu Prihandini Peni Wahyu Prihandini Permadi, Edy Prihandini, Peni Wahyu Puguh Surjowardojo Rakhmad Perkasa Harahap Retno Budi Lestari Retno Budi Lestari Rinanti, Rosyida Fajri Ristito Widodo Ristyawan, Mochammad Ridwan Sari, Apriliana Putri Zahara Nafsina Luvita Sari, Aprilianna Putri Zahara Nafsina Luvita Sitaresmi, Pradita Iustitia Sri Susilowati Suyadi Suyadi Suyatno Suyatno Tatan Kostaman Tri Eko Susilorini Utin Yulia Via Anggi Agustin Vindo Lesmana Wahidin Yoga Febriyanto Wahyuni Indah Wulansari Wike andre Septian Woro Busono, Woro Yashinta Salsabila zakiya tulyaqin Zultinur Muttaqin