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THE INHIBITORY ACTIVITY OF KELAKAI LEAF EXTRACT AGAINST THE GROWTH OF Porphyromonas gingivalis ATCC® 33277™ Destri Khusnul Khotimah; I Wayan Arya Krishnawan Firdaus; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 5, No 2 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i2.8945

Abstract

ABSTRACTBackground: Chronic periodontitis is an infectious disease that causes damage on periodontal ligament and alveolar bone. The severity of periodontitis is caused by several types of bacterial species which one of them is Porphyromonas gingivalis bacteria with a prevalence of 85% in oral cavity. The extract of kelakai leaf contained antibacterial in the form of flavonoid, alkaloid, tannin, and steroid. Flavonoid consists of some chemical compounds which is one of them is quercetin. The level of quercetin in kelakai leaf is 503.56 mgQE/g. From some secondary metabolites, kelakai leaf has inhibitory power toward gram negative bacterial, Porphyromonas gingivalis. Objective: This research was intended to know the activity of inhibitory power of kelakai leaf toward Porphyromonas gingivalis bacteria. Method: This research was an experimental research consisted of 5 experimental groups that were group of kelakai leaf extract on the concentrations of 100 mh/ml, 75 mg/ml, 50mg/ml, and 25 mg/ml and the control group (0.2% chlorhexidine). Each treatment was done in 4 repetitions. The test of inhibitory power used diffusion method by measuring the inhibitory zone around the growth of Porphyromonas gingivalis on Mueller Hinton Agar media. The data were analyzed by using One Way Anova 95% and then continued with LSD. Results: Based on the LSD test, it was known that the extract of Kelakai leaf had inhibitor power activity toward Porphyromonas gingivalis. The highest inhibitory zone was on the concentration of 100 mg/ml with inhibitory zone of 14.61 mm. Conclusion: The extract of kelakai leaf had inhibitory power activity toward Porphyromonas gingivalis bacteria in vitro. Keywords: 0.2% chlorhexidine, Diffusion method, Inhibitory power, Stenochlaena palustris extract, Porphyromonas gingivalis.
EFEKTIVITAS ANTIFUNGI EKSTRAK METANOL BATANG PISANG MAULI (Musa acuminata) DAN Chlorhexidine gluconate 0,2% TERHADAP Candida albicans Dita Permatasari; Lia Yulia Budiarti; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 1, No 1 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i1.409

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ABSTRACT Background: Mauli banana stem extract has antifungal effectiveness against Candida albicans because it has flavonoid, saponin, and tannin substances. Chlorhexidine gluconate 0,2% is widely used to treat oral candidiasis caused by Candida albicans fungal infection. Chlorhexidine gluconate 0,2% is known to have caused side effects. Purpose: The purpose of this study is to knowing the antifungal effectiveness differences between 25%, 80%, 100% mauli stem methanol extracts and chlorhexidine gluconate 0,2% against Candida albicans. Methods: This study was true experimental with post test-only with control group design consisted of 4 treatment groups. Treatment group I,II,III each was given 25%, 80%, 100% mauli banana stem methanol extract respectively and treatment group IV was given chlorhexidine gluconate 0,2% as positive control group. The repetition of each treatment was 7 times. Antifungal effectiveness was assessed by measuring inhibition zone of Candida albicans growth using diffusion method. Results: This research showed that mean inhibition zone of treatment group I (13 mm), treatment group II (17 mm), treatment group III (19 mm), and treatment group IV (21 mm). One-way Anova test indicated that there was significant difference between 25%, 80%, 100% mauli banana stem methanol extracts and chlorhexidine gluconate 0,2%. Conclusion: Based on the Post-hoc Bonferroni test, it can be concluded that 100% concentration mauli banana stem methanol extract had more prominent antifungal effectiveness than 25% and 80% against Candida albicans but still less effective than chlorhexidine gluconate 0,2%.Keywords: mauli banana stem extract, antifungal effectiveness, Candida albicans, chlorhexidine gluconate 0,2%ABSTRAK Latar Belakang: Ekstrak batang pisang mauli memiliki efektivitas antifungi terhadap Candida albicans karena mengandung senyawa flavonoid, saponin, dan tanin. Chlorhexidine gluconate 0,2% biasanya digunakan untuk mengobati kandidiasis oral yang disebabkan oleh infeksi jamur Candida albicans. Chlorhexidine gluconate 0,2% diketahui dapat menimbulkan efek samping. Tujuan: Tujuan penelitian ini adalah untuk mengetahui perbedaan efektivitas antifungi ekstrak metanol batang pisang mauli konsentrasi 25%, 80%, 100% dan chlorhexidine gluconate 0,2% terhadap Candida albicans. Metode: Penelitian ini merupakan penelitian eksperimental murni dengan post test-only with control group design yang terdiri dari 4 perlakuan. Perlakuan I,II,III masing-masing diberikan ekstrak metanol batang pisang mauli konsentrasi 25%, 80%, 100% dan perlakuan IV diberikan chlorhexidine gluconate 0,2% sebagai kontrol positif. Jumlah pengulangan setiap perlakuan adalah 7 kali. Efektivitas antifungi dinilai dari mengukur zona hambat pertumbuhan Candida albicans dengan metode difusi. Hasil: Penelitian ini menunjukkan bahwa rata-rata zona hambat yang terbentuk pada perlakuan I (13 mm), perlakuan II (17 mm), perlakuan III (19 mm), dan perlakuan IV (21 mm). Uji Oneway Anova menunjukkan bahwa terdapat perbedaan bermakna antara efektivitas antifungi ekstrak methanol batang pisang mauli konsentrasi 25%, 80%, 100% dan chlorhexidine gluconate 0,2%.Kesimpulan: Berdasarkan uji Post-hoc Bonferroni dapat disimpulkan bahwa efektivitas antifungi ekstrak metanol batang pisang mauli konsentrasi 100% lebih besar dibandingkan 25% dan 80%, tetapi masih kurang efektif dengan chlorhexidine gluconate 0,2%.
EXFOLIATIVE CHEILITIS (Case report) I Wayan Arya K. Firdaus; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6169

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Background : Exfoliative Cheilitis is defined as a chronic inflammatory disorder of the vermillion border of the lips, which is characterized by the persistent formation of scales and crusts. Underlying stress or psychiatric conditions may cause or exacerbate exfoliative cheilitis. Purpose : This paper reports the mechanisme of stress as the predisposing factor of exfoliative cheilitis. Case : The patient had been suffering the desquamation, dryness, and tenderness of lips for three months. The disease was not treated by any drugs. She consumed the multivitamin then ate more fruits and vegetables, but it was not getting well. Case Management : The anamnesis results that she had an allergy of some foods and never got serious diseases. There was story of biting and picking of lips, when she was getting stress. She was reffered by oral medicine specialist to get the complete blood count and Ig E test, but all of the results were normal. The final diagnosis was Exfoliative cheilitis, and the differential diagnosis was Allergic Stomatitis. She was prescibed triamcinolone acetonide 0,1% topically on her lips for three times until seven days. The instruction to patient were drinking much water and avoiding her habit of biting and picking of lips. Conclusion : Severe tress will trigger more proinflamation cytokines to increase epidermal growth factor (EGF) of oral mucous, so that caused unremitting desquamation.
MANAGEMENT OF VARICELLA ZOSTER IN ADULT PATIENT (Case report) Maharani Laillyza Apriasari; Selviana Rizky Pramitha
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6184

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Background: Varicella zoster is a self-limiting disease arising from acute primary infection in skin and mucosa. This disease frequently occurs in children. Its emergence in adult patient may aggravate the symptoms. Purpose: To elaborate the management of Varicella zoster in adult patient. Case: 40 years old female was presented with lips and mouth ulcers for the past two days. Prior to ulceration, itchy watery wheals were manifested on her face and continuously spread to the whole body. Patient confirmed that she experienced this condition for the first time and initially given Acyclovir 400 mg and Paracetamol 500 mg from Community Health Centre healthcare provider. After regularly consuming prescribed drugs for four days, fever and ulcerations had not been subsided. Case management: Intraoral examination displayed one to two milimetre painful multiple ulcers, yellow in color, with regular margin surrounded by erythema on left upper lip, lower lip, and posterior palatum mucosa. Patient was subsequently prescribed with Acyclovir tablet 800 mg four times a day, Ibuprofen tablet 400 mg three times a day, multivitamin B complex and C caplet once a day and aloevera-containing mouthwash to be applied thrice daily. Conclusion: Varicella zoster in patients older than thirteen should be given antiviral therapy to prevent future complications.
THE EFFECT OF TOMAN FISH EXTRACT (Channa Micropeltes) ONNEUTROPHILIN DIABETES MELLITUSWOUND HEALING (In Vivo Study in the Back of Male Wistar Mice (Ratus Novergicus) Nuril Fajriani; Amy Nindia Carabelly; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4613

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Background: Toman fishcontains albumin and unsaturated fatty acids which can be an alternative of diabetes mellitus wounds healing process. People with diabetes mellitus have their neutrophil decreased. Albumin increases the number of neutrophil. Unsaturated fatty acid decreases the number of neutrophil. Purpose: To prove the effect of toman fish extract of 16 ml/Kg BW mice orally on thenumber of neutrophil in day 2, 4, and 8 of male wistarmice with diabetic wound. Materials and Method: This research applied pure experimental research method with posttest only control design. This research took 36 wistarmice which weredivided into negative control group which is given BR2 feed, treatment group which is giventoman fish extract in 16 mL/Kg BW mice and positive control group which is given haruan fish extract in 13.54 mL/Kg BW mice. The Result: One-way ANOVAshowedthat there wassignificant differenceof all groups in day 2 (p=0.000), day 4 (p=0.000) and day 8 (p=0.000). Post-Hoc LSD test for the average of neutrophil number showedthat there was significant difference (p<0.05) between the negative control group, treatment group and the positive control group. Conclusion: Toman fish extract has an effect which increasesthe number ofneutrophil in day 2 and decreasesthe number of neutrophil in day 4 and 8 on the healing of diabetic wound in mice.
MANAGEMENT OF ACUTE BACTERIAL SIALADENITIS (Case Report) I Wayan Arya Krishnawan Firdaus; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4592

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Background : Sialadenitis is the inflammation of the salivary gland. Predisposing factors for acute sialadenitis include diabetes mellitus, hypothyroidism, renal failure, and Sjögren syndrome. The most common bacterial cause of acute bacterial sialadenitis is Staphylococcus aureus. Purpose : to report the management of Acute bacterial sialadenitis, especially the recommendation antibiotic for treatment of  Acute bacterial sialadenitis. Case : Woman, 34 years old,  suffered the  pain of left buccal especially when she was eating. There was a lowgrade fever. She has been suffering since 3 days ago, but she did not give any drugs. The intra oral examination showed  the stenoni of parotid gland  was swollen and painful, and  erythematous. The purulent discharge often was observed from the duct orifice when the gland was checked by palpation. The patient was diagnosed as  Acute Bacterial sialadenitis. Case management : Amoxycillin caplet 500 mg three times a day,  Ibuprofen caplet 400 mg three times a day, and oral rinse contains aloevera gargle three times a day for seven days,  then the instruction to patient took a lot of rehydration. After seven days, Cefadroxil capsul 500 mg two times  a day replaced amoxycillin caplet 500 mg three times a day. Conclusion : It can  be concluded that Cephalosporins display superior pharmacokinetics in saliva and cover the spectrum of all bacteria implicated in sialadenitis.
EFFECT OF Musa acuminate AND Ocimum basilicum MIXED EXTRACTS ON BIOACTIVE RESIN’S FLUORIDE RELEASE Kurnia Fatwati; Dewi Puspitasari; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8131

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Background: Bioactive composite resin was materials that are able to released fluoride when the intra oral pH value drops. Mouthwash is one of the triggers for changes in pH of the oral cavity. The many side effects caused by chemical mouthwashes make herbal plants an alternative to natural mouthwash. Traditional plants that have the potential to be used as mouthwash include of Mauli Banana (Musa acuminata) and basil (Ocimum basilicum L.).Objective: To analyze the effect of Mauli banana stem (Musa acuminate) and basil leaves (Ocimum basilicum) mixed extracts on fluoride (F) release from bioactive resin. Method: Thirty specimens (15mm diameter x 1mm thick; n=5/group) were fabricated using Activa ™ Bioactive Restorative (Pulpdent). Mauli banana stem and basil leaves extracts mixture at the concentration of 25%, 50%, 75%, and 100% were respectively exerted as treatment groups while 0.2% Chlorhexidine Gluconate (CHX) and aquadest employed as control groups. Bioactive resin immersion was performed for 7 days in 37oC incubator prior to the measurement of F release using pH meter Lutron (208) TL Lutron (Taiwan). Results: One Way Anova and Post Hoc Dunnets-T3 not only depicted the significant differences among treatment groupprior tontly easurement ofrable bioactive resin durability in the oral cavity. on f of 50% (3.22±0.46ppm), 75% (2.31±0,45ppm), 100% (2.29±0.46ppm), but also control group CHX (2.31±0,45ppm), and aquadest (1.85±0.91ppm) compared to 25% treatment group (6.00±0.87ppm).Conclusion: There is a significant effect of Mauli Banana stem and basil leaves extract mixture on bioactive resin F release. Increasing concentration may reduce the number of F release yet achieve a preferable bioactive resin durability in the oral cavity.
THE EFFECT OF Musa acuminata AND Ocimum basilicum MIXED EXTRACTS TO THE SURFACE HARDNESS OF BIOACTIVE COMPOSITE RESIN Dewi Puspitasari; Maharani Laillyza Apriasari; Dewi Rahayu; Priyawan Rachmadi
Dentino : Jurnal Kedokteran Gigi Vol 5, No 2 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i2.8970

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ABSTRACTBackgrounds: The use of mouthwash daily may influence the mechanical properties of bioactive composite resin as a dental restoration, one of the mechanical properties is surface hardness. Herbal mouthwash can be used to minimize the side effect of mouthwash that contain alcohol. Mauli banana stem and basil leaf extracts can be produced into herbal mouthwash. Methods: Thirty specimens (10mm diameter x 2mm thick; n=5/group) bioactive composite resins were immersed in a mixed solution of Mauli banana and basil leaf extracts with concentration of 25%, 50%, 75 %%, 100%, the control group of chlorhexidine gluconate 0.2% and aquadest for 7 days in 37oC. Measurement of the surface hardness was using the Vickers Microhardness Tester with 100 gf load for 15 seconds. Results: One Way ANOVA and Post Hoc Bonferroni exhibited the significant differences (p <0.05) in hardness values between the specimens that immersed in the mixed solution Mauli banana and Basil leaf extracts, which in the concentration of 100% (4,49 ± 4,61 VHN) compared with 25% (38,20 ± 2,58 VHN), 50% (41,40 ± 3,84 VHN) and 75% concentration (40,40 ± 3,55 VHN). There was no siginificant difference (p >0.05) between specimens immersed in CHX, aquadest and the mixed solution of mauli banana and basil leaf extracts in all concentration. Conclusion: There is no change in bioactive composite resin surface hardness after immersed in the mixed solution of Mauli Banana stem (Musa acuminata) and Basil leaf (Ocimum basilicum) extracts.Keywords: Basil leaf extract, Bioactive resin, Mauli Banana stem extract, Surface hardness
THE EFFECT OF MAULI BANANA STEM (Musa acuminata) AND BASIL LEAF (Ocimum basilicum L.) EXTRACT ON THE VALUE OF DIAMETRAL TENSILE STRENGTH OF BIOACTIVE COMPOSITE RESIN Dewi Puspitasari; Annisa Aulia Rahmah; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 7, No 1 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i1.13106

Abstract

Background: Chemical contents in mouthwash could cause several side effects in the oral cavity, hence natural ingredients were used such as Mauli banana stem and basil leaf extract. Mouthwash exposure in the oral cavity affects composite resin, one of it is a bioactive composite resin which has advantages over conventional ones. The contact of mouthwash with bioactive composite resin affects strength properties. Purpose: To analyse the effect of Mauli banana stem and basil extract on the diametral tensile strength value of the bioactive composite resin. Method: Thirty specimens (diameter 6mm x thickness 3mm; n=5/ group) were made using Activa™ Bioactive Restorative (Pulpdent) and immersed in the following group of 25%, 50%, 75% and 100% Mauli banana stem and basil leaf extract as a treatment groups, chlorhexidine gluconate 0,2 % (CHX) and aquadest as a control groups for 24 hours in the incubator at 37oC. Diametral tensile strength value was measured by Universal Testing Machine (Tokyo Testing Machine MFG CO.LTD) with 0,5 mm/minute crosshead speed and 250 kgF load cell. Result: One Way Anova and Post Hoc Bonferroni show there is a significant difference between treatment groups of 25% extract (41,33±6,20 MPa) and treatment groups of 100 % extract (55,06±6,42 MPa). Meanwhile, there is no significant difference for other groups. Conclusion: There is a significant effect of Mauli banana stem and basil leaf extract on the diametral tensile strength value of bioactive composite resin.
THE EFFECT OF MAULI BANANA STEM EXTRACT GEL (Musa acuminata) APPLICATION IN CONCENTRATION OF 37.5% ON EPITHELIAL THICKNESS (Research report) Monica Thiodora Limay; Maharani Laillyza Apriasari; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6166

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Background: Injury is a state of damage or loss of tissue that can be caused by physical action. Plants that can be used in wound healing is mauli banana stem.  Mauli banana stem extract gel concentration of 37.5% contains tannins and saponins which have potential as immunomodulators. Previous research has stated that mauli banana stem extract gel concentration of 37.5% can cure traumatic ulcers. Purpose: To prove the effect of mauli banana stem (Musa acuminate) extract gel application in concentration of 37.5% on epithelial thickness in wistar rat’s (Rattus novergicus) oral mucosa lesions  on the 7th day of wound healing process. Method and material: This research was a true experimental research with posttest only control group design. It used 3 treatment groups consisted of a group which was given mauli banana stem (Musa acuminata) extract gel concentration of 37.5%, hydroxypropyl methylcellulose gel (HPMC) and patent drug containing Aloe vera extract gel. Results: The mean of  epithelial thickness in the wound healing process on the 7th day in treatment group, negative control group, and positive control grou were 119.1857 μm, 82.4563 μm, and 90,5937μm respectively . One-way Anova's test showed that there is a difference between the result of the treatment groups. Post hoc LSD test showed significant difference of epithelial thickness between mauli banana stem extract concentration of 37.5% group and hydroxypropyl methylcellulose gel (HPMC) group. There is no significant differerence between mauli banana stem extract gel concentration of 37.5% group and the patent drug containing Aloe vera extract gel group. Conclusion: The application of mauli banana stem extract gel in concentration of 37.5% can increase epithelial thickness in oral mucosa on the 7th day of wound healing process
Co-Authors Adhiya, Geyanina Melda Agung Satria Wardhana Almadina Latanza Amy Nindia Carabelly Anita Fitriani Annisa Vara Nurdianty Aprilyani, Nur Ariska Endariantari Asphia Rahmah Astuti, Nadia Dewi Aulia Azizah Aulia Rahma Annisa Bagus Soebadi Beta Widya Oktiani Beta Widya Oktiani Cane Lukisari, Cane Debby Saputera, Debby Destri Khusnul Khotimah Dewi Nurdiana Dewi Puspitasari Dewi Puspitasari Dewi Rahayu Diah Savitri Ernawati Dita Permatasari Dita Permatasari Dwi Kurniawan, Fajar Kusuma Dwi Setyaningtyas, Dwi Eko Suhartono Eriwati, Yosi Kusuma Erni Marlina Fajriani, Nuril Firdaus, I Wayan Arya Krishnawan Fuady, Ridwan Ichshalul Hadi Waskito Hamdani, Riky Hatta, Isnur Hening Tuti Hendarti Husma, Emma Annahal I Wayan Arya K. Firdaus I Wayan Arya Khrisnawan Firdaus I Wayan Arya Krishnawan Firdaus I Wayan Arya Krisnawan Firdaus Ichrom Nahzi, Muhammad Yanuar Ika Kustiyah Oktaviyanti Khairunnisa Amalia Pratami Komarudin, Koko Krishnawan Firdaus, I Wayan Arya Kurnia Fatwati Lia Yulia Budiarti M. Jusri M. Ridhotama Wibowo Maria Tanumihardja, Maria Marina Arum Syahadati Mashud, Mustain Melisa Budipramana, Melisa Moh. Ihsan Rifasanto Monica Thiodora Limay Monita Valentine Silalahi Muhammad Ikhlasul Amal Sangadji MUHAMMAD RAYHAN Nadia Dewi Astuti Nafi’ah, Nafi’ah Nor Kamalia Novie Aprianti Novie Aprianti, Novie Nurashfia Nurashfia Nuril Fajriani Nurrahman, Tri Nurul Ikhsani Umar Pramitha, Selviana Rizky Priyawan Rachmadi Rahmadella, Afifah Renie Kumala Dewi Retno Andriati, Retno Retno Pudji Rahayu Rifasanto, Moh. Ihsan Saldy Rizky Saputra Saldy Rizky Saputra, Saldy Rizky Sari, Tiara Intan Permata Sherli Diana Silalahi, Monita Valentine Sundah, Serenada Audria Tri Putri, Deby Kania Utami, Juliyatin Putri Wenda Fitriati Noor Wenda Fitriati Noor, Wenda Fitriati Yunike Christanti Yusnida, Resha Yusrinie Wasiaturrahmah