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Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Abstract

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability
STUDI STRUKTUR DAN KOMUNITAS PLANKTON DI SUNGAI JEMBAYAN KECAMATAN LOA KULU, KUTAI KARTANEGARA Patang, Nurfadilah A.; Hendra, Medi; Kurniati, Reni; Fudiantoro, Aldi; Hariani, Nova
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 15 No 2 (2023): BIOPROSPEK: Jurnal Ilmiah Biologi
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v15i2.1212

Abstract

Penelitian ini bertujuan untuk mengetahui kondisi lingkungan (fisika dan kimia) serta struktur komunitas plankton di sungai Jembayan kecamatan Loa Kulu kabupaten Kutai Kartanegara. Teknik pengambilan sampel menggunakan random sampling. Data yang diperoleh diolah dan disajikan dalam bentuk kelimpahan plankton, indeks keanekaragaman, indeks keseragaman dan indeks dominasi. Selama penelitian diperoleh rentang kecerahan termasuk dalam kategori perairan keruh, rentang suhu menunjukkan rentang cukup baik untuk pertumbuhan biota perairan, rentang salinitas yang menunjukkan air tawar, rentang TDS termasuk dalam rentang normal dan rentang pH termasuk dalam kategori dari pH rendah. Selama penelitian ditemukan plankton yang termasuk dalam kelas 10 yaitu Bacillariophyceae, Chlorophyceae, Crustacea, Cyanophyceae, Dictyophyceae, Euglenophyceae, Monogononta, Rhizopoda, Rotifera dan Zygnematophyceae. Selama penelitian kelimpahan plankton di Stasiun 1 sebanyak 15.050 individu/Liter, di Stasiun 2 sebanyak 20.819 individu/Liter, di stasiun 3 sebanyak 25.627 individu/Liter. Indeks Keanekaragaman (H') pengamatan berkisar antara 2,709 hingga 3,064 dengan keanekaragaman tertinggi terdapat pada stasiun dua sore sebesar 3,064 dan terendah pada Stasiun 1. Semua stasiun penelitian mempunyai tingkat keanekaragaman sedang. Indeks keseragaman (E) berkisar antara 0,831 sampai 0,940 menunjukkan bahwa jumlah individu tidak merata atau tidak seragam. Indeks dominasi (D) yang berkisar antara 0,057 hingga 0,093 menunjukkan bahwa tidak terdapat jenis plankton tertentu yang mendominasi perairan pada setiap stasiun pengamatan.
Keragaman Kultivar Lokal Ubi Jalar (Ipomoea batatas (L.) Lam) di Kabupaten Kutai Timur-Kalimantan Timur, Indonesia Maysavitrie Citra Wijayanti Kristianto; Medi Hendra; Linda Oktavianingsih
Al-Kauniyah: Jurnal Biologi Vol 17, No 1 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i1.30539

Abstract

 AbstrakUbi jalar (Ipomoea batatas (L.) Lam) merupakan salah satu tanaman pangan yang memiliki banyak manfaat di beberapa daerah di Indonesia termasuk di Kabupaten Kutai Timur. Informasi tentang keragaman morfologi kultivar lokal ubi jalar diperlukan untuk pemuliaan tanaman ke depannya. Penelitian dilakukan pada Maret-Juli 2021 untuk mengetahui keragaman dan hubungan kekerabatan kultivar lokal ubi jalar berdasarkan karakter morfologi. Kultivar lokal ubi jalar diambil secara purposive sampling melalui metode jelajah di 11 kecamatan, Kabupaten Kutai Timur. Karakterisasi morfologi menggunakan 32 karakter berdasarkan panduan International Board for Plant Genetic Resource (IBPGR). Analisis klaster untuk melihat hubungan kekerabatan antar kultivar menggunakan software MVSP versi 3.1. Hasil penelitian menunjukkan terdapat keragaman kultivar lokal ubi jalar yang ada di Kutai Timur berdasarkan karakter daun (bentuk, tipe cuping), umbi (bentuk, warna daging), bunga (bentuk dan warna). Hasil analisis klaster membentuk dua kelompok besar dari 23 aksesi kultivar lokal ubi jalar yang ditemukan dengan koefisien 9,671. Kelompok A dengan satu anggota, yaitu kultivar lokal 9 (Gei Meng Hom) yang terpisah dari kultivar lokal lainnya berdasarkan karakter umbi. Kultivar ini dibudidayakan oleh masyarakat asli Kalimantan (suku Dayak Wehea). Kelompok B terdiri dari 22 aksesi kultivar lokal yang dapat dikelompokkan berdasarkan karakter daun.AbstractSweet potato (Ipomoea batatas (L.) Lam) is a food crop that has many benefits in several regions in Indonesia, including East Kutai Regency. Information about the morphological diversity of local sweet potato cultivars is needed for future plant breeding. The research was conducted from March-July 2021 with the aim of determining the diversity and relationships of local sweet potato cultivars based on morphological characters. The collection of local sweet potato cultivars was carried out using purposive sampling using the roaming method in 11 sub-districts, East Kutai Regency. Morphological characterization uses 32 characters based on the International Board for Plant Genetic Resources (IBPGR) guidelines. Cluster analysis to see the kinship relationships formed between local sweet potato cultivars using MVSP software version 3.1. The results of the research show the diversity of local sweet potato cultivars in East Kutai based on leaf characters (shape, lobe type), tubers (shape, flesh color), flowers (shape and color), as well as forming two large groups based on the results of cluster analysis on 23 accessions which was found with a coefficient of 9.671. Group A with one member, namely local cultivar 9 (Gei Meng Hom) which is separated from other local cultivars based on tuber characteristics and is a local cultivar of sweet potato cultivated by the indigenous people of Kalimantan (Wehea Dayak tribe). Group B consists of 22 local cultivar accessions resulting from community cultivation which can be grouped based on leaf characteristics.  
Genetic diversity of local sweet potato cultivars (Ipomoea batatas [L.] Lam.) from Bulungan, North Kalimantan based on morphological characters OKTAVIANINGSIH, LINDA; FITRIANI, FITRIANI; HENDRA, MEDI; ARIF, MUHAMMAD FAUZI
Jurnal Natural Volume 24 Number 3, October 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i3.40568

Abstract

Indonesia is one of the centers of sweet potato diversity worldwide. Sweet potatoes have the potential to be developed for food consumption. This research aims to determine the diversity of sweet potato cultivars, their morphological characteristics, and the relationships between local sweet potato cultivars in Bulungan Regency, North Kalimantan. The local sweet potato cultivars were selected using purposive sampling with the exploration method. The morphological characterization of the samples is based on IBPGR (International Board for Plant Genetic Resources) standards. Cluster analysis was conducted to identify the relationships using MVSP software version 3.1. The research identified 27 local sweet potato cultivars in Bulungan Regency. Morphological characterization revealed 41 characters that can differentiate each local sweet potato cultivar. The most influential characteristics include leaf shape, flower surface texture, the position of the stigma, flower surface color, back petal color, center shape and the number of lobes, primary leaf vein color, lobe type, main leaf bone color, stem internode length, and plant type. A flower shape that had not been discovered before, a pentagonal shape with curves, was identified. Cluster analysis divided the cultivars into two major groups with a coefficient range of 0.593 to 0.888 (59.3 to 88.8%). Cluster A consisted of one member, LK-7 (purple sweet potato), while Cluster B consisted of several subgroups with 26 local cultivars. The people of Bulungan Regency use these local cultivars for food, animal feed, and medicinal purposes.
PENGARUH LAMA PEREBUSAN SIMPLISIA DAUN APAH (Albertisia papuana Becc.) YANG DIGUNAKAN SEBAGAI PENYEDAP MAKANAN OLEH MASYARAKAT KAB. TANA TIDUNG TERHADAP ANGKA CEMARAN MIKROBA Rosnah Rosnah; Medi Hendra; Eko Kusumawati
Jurnal Ilmiah Manuntung Vol 2 No 1 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v2i1.41

Abstract

The purpose of this study is to determine the effect of long simplicia Apah’s leaves (Albertisia papuana Becc.) that’s used as a food’s flavoring by people of Kab. Tana Tidung against microbial contamination numbers. The design of research used a pattern completely randomized design (RAL), with a dilution series of samples 10-8, 10-9 and 10-10 and different boiling’s level (5, 10, 15, 20 and 25 minutes). The results obtained that the longer of boiling process is used, then the less the number of microbial contamination of colony growth. Average number of microbial contamination in samples in the boiling of 5 minutes is 92 x 1010 microbial colonies, in boiling 10 minutes is 87 x 1010 microbial colonies, in boiling 15 minutes is 56 x 1010 microbial colonies, in boiling 20 min is 44 x 1010 colonies of microbes and the boiling simplicia infuse for 25 minutes is 33 x 1010 colonies of microbes. Based of Analysis variance variety in each treatment showed that in boiling 20 minutes and 25 minutes showed real significant difference (p <0.05), but the boiling 5, 10 and 15 minutes there was no significant difference (P> 0.05). The results of the Anova, showed that in boiling treatment of simplicia Apah’s leaves (Albertisia papuana Becc.) significantly affects the growth of microbial colonies. Analysis of Least Significant Difference (LSD) at the level of 95%, the result that the effects of long boiling the simplicia Apah’s leaves (Albertisia papuana Becc.) which is used as a food flavoring by people of Kab. Tana Tidung, significant effect on the numbers of microbial contamination
UJI PENDAHULUAN DAUN TENEM Litsea cubeba (Lour.) Pers Risa Supriningrum; Medi Hendra; Hendri Misak
Jurnal Ilmiah Manuntung Vol 2 No 1 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v2i1.42

Abstract

Litsea cubeba (Lour Pers.) has been used by the Dayak community Lundayeh and Kenyah, the village of Long Berang, District Mentarang, North Borneo, as drug . Leaves and bark used as medicine for diarrhea. Preliminary data concerning the characteristics and content of secondary metabolites has not been reported. This study aims to determine the organoleptic, macroscopic,  microscopic characteristics and content of secondary metabolites tenem leaves. The results showed that the leaf tenem have organoleptic characteristics typical aromatic smell like citrus (lemon), taste slightly bitter, slightly spicy, chelates. Macroscopic characteristics in the form of a single leaf, lanceolate, elongated, tapered leaf tip, leaf base tapering, bone pinnate leaves, upper surface shiny, lower surface dull and slightly hairy, color light green to dark green. Microscopy test results showed fragments of sklereid, oil cell, stomata type of parasitic, non-glandular trichomes. Simplicia tenem leaf powder contains alkaloids, tannins and terpenoids. The ethanol extract of leaves tenem containing compounds, terpenoids, tannins, alkaloids
INVENTARISASI DAN IDENTIFIKASI TUMBUHAN OBAT SUB ETNIS KUTAI DESA GENTING TANAH KECAMATAN KEMBANG JANGGUT KABUPATEN KUTAI KARTANEGARA KALIMANTAN TIMUR Anita Apriliana; Medi Hendra; Atila Sapitri
Jurnal Ilmiah Manuntung Vol 2 No 1 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v2i1.54

Abstract

Indonesia has rich tropical forests will be diverse plants that can be used as a traditional medicine, ranging from roots, stems, leaves, until all fruit is of great value and can be used as a medicine for the health as well as from different tribe who were in Indonesia. Since old time sub etnic Kutai using different types of plants as a medicine because the lack of medical personnel and health care center in curing diseases. This study aims to find out the types of medicinal herbs used by tribes. The results there were 39 Medicinal plants used by tribes that is Serai, Sirih, Tumbuh daging, Kepius, Patah tulang, Kunyit root, Senuru , Mali wood, Prepat wood, lupun root, Kecubung, Terung pipit, Gelinggang, Beribit grass, Syurga leaf, Tapis bongol, Nyiur ongo, Singgah bini wood, Penggel habeng, Serkemudi grass, Tunjuk langit root, Kemet hitam, Pacar beleng, penggel putih, Ketotol grass, Kemantrah, Sahung wood, Tajerebo, Cula adem, Risip leaf, Lalang, Sampai root, Cancut haji jewa, Mersesat leaf, Lekop leaf, Kuku tupai, Kempis root, Kemet putih and Kedeme
KEANEKARAGAMAN DAN KOMPOSISI IKAN PADA KONDISI PASANG DAN SURUT DI MUARA SUNGAI KELAY KABUPATEN BERAU KALIMANTAN TIMUR Yudistira, Yudistira; Jusmaldi, Jusmaldi; Hendra, Medi
Jurnal Pendidikan Matematika dan IPA Vol 10, No 2 (2019): Juli 2019
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (798.015 KB) | DOI: 10.26418/jpmipa.v10i2.25868

Abstract

Research on diversity and composition of fish at low tide and high tide condition in downstream Kelay River, District Berau, East Kalimantan is not yet know. The purpose of this research was to determine diversity and composition of fish that present at low tide and high tide condition in downstream Kelay River. In research using purposive sampling method and various sizes gill nets used. Results of this research shows that fish were collected at three locations sampling in downstream Kelay River consist of 14 species, 9 families, and 953 individuals. Based on the number of species, diversity Shannon-Wiener index and dominance index has stable of communities, with high value of diversity index (H'=1.975), high evenness index (E=0.749), and low value of dominance index  (D= 0.173). The number of fish species found at low tide were 11 species and at high tide were 12 species. The similarity index of fish species at low tide and high tide condition were 64.3%.
Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Abstract

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability
Pengenalan Pengelolaan Sampah Sejak Dini Pada Sekolah Dasar di Kecamatan Kota Bangun Mislan, Mislan; Anwar, Yaskinul; Murti, Sus Tri; Lariman, Lariman; Hendra, Medi; Heryadi, Eko
Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Vol. 5 No. 2 (2025): Mei 2025
Publisher : Cipta Media Harmoni

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53624/kontribusi.v5i2.587

Abstract

Latar Belakang: Sampah merupakan permasalah yang belum terselesaikan terlebih lagi berkaitan dengan prilaku masyarakat. Pengenalan pengelolaan sampah perlu ditanamkan sejak dini pada anak – anak, supaya lingkungan kedepannya dapat lestari. Tujuan: Pengabdian ini memberikan pengenalan pengelolaan sampah kepada siswa – siswa sekolah dasar terkait pemilahan dan pengelolaan sampah. Kegiatan ini dilakukan mulai januari sampai maret 2023 di SDN 003 dan 004 Kota Bangun. Metode: Pengenalan pengelolaan sampah dilakukan dengan cara workshop yang dipadukan dengan ceramah dan games dalam mengenalkan cara – cara pengelolaan sampah, khususnya dalam melakukan pemilahan sampah. Hasil: Berdasarkan hasil evaluasi menunjukkan siswa dan pihak sekolah sudah mulai melakukan pengelolaan sampah secara sederhana, terutama untuk sampah plastik, walaupun masih ada beberapa kesalahan dalam pemilahan jenis sampah. Kesimpulan: Dengan melatih pengelolaan sampah sejak dini ini, diharapkan dapat meningkatkan kebersihan dan meningkatkan kelestarian lingkungan sekitar Sungai dan Danau Kaskade Mahakam.
Co-Authors Ahmad Randy Aisya, Sitti Akhmad Darmawan Al Husna, Riezdqhy Amalina F. Anita Apriliana Anita Apriliana, Anita Anwar, Yaskinul Ardiansyah, DT Ariani, Novita Arief Hidayat Arum, Elis Seftia Atila Sapitri Auliana B, Dian Aspiyani Chairul Saleh Crisdayanti, Noviana Daniel Happy Putra Daryadi A, Muhammad DEWI RAHMAWATI Dharma, Bodhi Dijan Sunar Rukmi Dina Hayati Putri Dwi Susanto Dwi Susanto Eko Heryadi eko heryadi, eko Eko Kusumawati Ervinda Yuliatin Eva Marliana, Eva Fitriani Fitriani Freminci Fudiantoro, Aldi Hartono, Thirza Larence Helda Sason Hendri Misak Hendris Hendarsyah Kurniawan, Hendris Hendarsyah Husdiati Imam Rosadi Jamun, Rosalia Jusmaldi Jusmaldi Kadek Subagiada, Kadek Kurniati, Reni Kusumawati, Eko Lariman Lariman Lariman, Lariman Linda Oktavianingsih Linda Oktavianingsih Lonita Lonita Luh Putu Ratna Sundari Martafola, Cristina Maulidiyah Maysavitrie Citra Wijayanti Kristianto Megawati Megawati - Minarti Minarti Misak, Hendri Mislan Mislan Mislan Muhaenal Muhammad Basrowi Muhammad Fauzi Arif Muhammad Fauzi Arif Muhammad Nurdin Mukhlis munasirah, Munasirah Murti, Sus Tri Nikmahtulhaniah Ayu Wulandari Nova Hariani Nur Miati Nuramelia, Nadira Oktaviani, Martha Patang, Nurfadilah A. Primahana, Gian Pujianti, Eva Putri, Fiorhentina Rahayu, Ndaruning Tri Rahmatullah, Kukuh Risa Supriningrum Rivaldi, Ahmad Rofi, Lintang Maula Rosnah Rosnah Rosnah, Rosnah Salsabillah, Natasya Irma Nur Sapitri, Atila Sarah Sarah Sasmitha Savitri, Pitaloka Alif Siti Hadijah Sudrajat Sudrajat SUKIRNO Sukirno Sukirno Surianto Effendi Sus Tri Murti syafrizal Syalsabila, Adelia Utami, Alya Nindityas Widya Fajariani Winata, Ari Yanti Puspita Sari Yanti Puspita Sari Yaskinul Anwar Yudistira Yudistira Yuliatin, Ervinda Zaini, Mulia