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All Journal Jurnal Riset Kesehatan THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Prima Jurnal Mandala Pharmacon Indonesia Jurnal Analis Medika Biosains (JAMBS) Jurnal Pengabdian Masyarakat Multidisiplin Jurnal Pengabdian Masyarakat Sasambo Quality : Jurnal Kesehatan Jurnal Kefarmasian Indonesia Journal of Indonesia Laboratory Students (JILTS) Jurnal Indonesia Mengabdi Galen: Jurnal Ilmu Farmasi dan Kesehatan
Dewi, Lale Budi Kusuma
POLTEKKES KEMENKES MATARAM
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Journal : Jurnal Kesehatan Prima

 1 
GOLD IMMUNOCHROMATOGRAPHIC ASSAY (GICA) SEBAGAI IMUNOSENSOR MENDETEKSI ANTIBODI Bacillus Anthracis PENYEBAB PENYAKIT ZOONOSIS Gunarti Gunarti; Yunan Jiwintarum; Awan Dramawan; Lale Budi Kusuma Dewi
Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v9i1.64

Abstract

Abstract: The purpose of this study was to identify patterns of protein antigens full / secretory antigens may be antigenic protein (PA) of Bacillus anthracis by the use of SDS-PAGE method (Laemmli) which can be used as bioreseptor on the test device fabrication method for detecting antibodies GICA of Bacillus anthracis , identify patterns of proteins that are antigenic protein (PA) or indicate the nature of the reactivity of Bacillus anthracis by using Western blotting and Determining the specificity and sensitivity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference. This study is a descriptive observational study. Research variable antigenic protein of Bacillus anthracis as bioreseptor and Antibodies from Bacillus anthracis as imunosenssor. The test prototype test device made using 20 sample with ELISA as a reference for calculating the sensitivity and specificity. The results of this study were identified band – band of 92 kDa, 68 kDa, 54 kDa, 43 kDa, 29 kDa and 18 kDa that can be used as bioreseptor on GICA, identified a pattern of proteins that are antigenic or indicate the nature of the reactivity of the Bacillus anthracis 68 kDa by Western blotting using the method, extract secretory antigen (SA) Bacillus anthracis can be used as a test device bioreseptor on GICA method for the detection of IgG antibodies against Bacillus anthracis and sensitivity and specificity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference each is 100% and 87.5%.
The Effect of Roasting Methods of Ingredients The Songgak Tribe Sasak Herbal on Active Compounds Using Gas Chromatography Mas Spectroscopy (Gc-Ms) Analysis) Agrijanti, Agrijanti; Kusuma Dewi, Lale Budi; Khusuma, Ari; Wardoyo, Eustachius Hagni
Jurnal Kesehatan Prima Vol 18, No 2 (2024): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v18i2.1540

Abstract

Songgak is a concoction of the Sasak tribe of Lombok in West Nusa Tenggara. It consists of coffee mixed with groundwood, cloves, black pepper, coriander, and nutmeg. The original recipe involves roasting the spices before mixing them with coffee, namely using a frying pan over a stove. The roasting process is carried out without controlling the temperature and roasting time, so the roasted ingredients need to be standardized(Dewi et  al., 2021). GCMS is a tool used to analyze compounds in samples using chemical separation methods, mainly chromatography. The compounds in the mixture are separated in a chromatographic column. The separation mechanism between several compounds occurs due to differences in the solubility values of each in the moving solvent and differences in the absorption of each of the competing compounds into the stationary phase (Gross, 2017). In this research, variations will be carried out on spices, namely T1 original treatment of roasting spices over fire for 8 minutes, T2 drying spices in sunlight, T3 oven for 5 minutes, T5 oven for 5 minutes, T4 oven for 15 minutes and T5 oven for 15 minutes. In the T4 treatment, namely oven for 10 minutes, the results of the analysis of the substance content with the most types, namely 20 types, were obtained. The active anti-microbial compound found in all treatments was Hexadecanoic acid, 2-hydroxy-1-(h)(C19 H38 O4) BM:330, which helps inhibit the growth of cancer cells. (National Library Medicine, n.d.). Octadecanoic acid (CAS) Stear (C18 H34 O2) BM:282 Anti-inflammatory, Antiandrogenic Cancer prevention, Hypocholesterolemia Dermatigenic, 5-Alpha reductase inhibitor, Anemiagenic, Insecticide (Natarajan et al., 2019) and Tetradecanoic acid (CAS) Myris (C14 H28 O2) BM:228 as anti-inflammatory and anti-nociceptive. 
The Effect of Roasting Methods of Ingredients The Songgak Tribe Sasak Herbal on Active Compounds Using Gas Chromatography Mas Spectroscopy (Gc-Ms) Analysis) Agrijanti, Agrijanti; Kusuma Dewi, Lale Budi; Khusuma, Ari; Wardoyo, Eustachius Hagni
Jurnal Kesehatan Prima Vol. 18 No. 2 (2024): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v18i2.1540

Abstract

Songgak is a concoction of the Sasak tribe of Lombok in West Nusa Tenggara. It consists of coffee mixed with groundwood, cloves, black pepper, coriander, and nutmeg. The original recipe involves roasting the spices before mixing them with coffee, namely using a frying pan over a stove. The roasting process is carried out without controlling the temperature and roasting time, so the roasted ingredients need to be standardized(Dewi et  al., 2021). GCMS is a tool used to analyze compounds in samples using chemical separation methods, mainly chromatography. The compounds in the mixture are separated in a chromatographic column. The separation mechanism between several compounds occurs due to differences in the solubility values of each in the moving solvent and differences in the absorption of each of the competing compounds into the stationary phase (Gross, 2017). In this research, variations will be carried out on spices, namely T1 original treatment of roasting spices over fire for 8 minutes, T2 drying spices in sunlight, T3 oven for 5 minutes, T5 oven for 5 minutes, T4 oven for 15 minutes and T5 oven for 15 minutes. In the T4 treatment, namely oven for 10 minutes, the results of the analysis of the substance content with the most types, namely 20 types, were obtained. The active anti-microbial compound found in all treatments was Hexadecanoic acid, 2-hydroxy-1-(h)(C19 H38 O4) BM:330, which helps inhibit the growth of cancer cells. (National Library Medicine, n.d.). Octadecanoic acid (CAS) Stear (C18 H34 O2) BM:282 Anti-inflammatory, Antiandrogenic Cancer prevention, Hypocholesterolemia Dermatigenic, 5-Alpha reductase inhibitor, Anemiagenic, Insecticide (Natarajan et al., 2019) and Tetradecanoic acid (CAS) Myris (C14 H28 O2) BM:228 as anti-inflammatory and anti-nociceptive. 
Co-Authors Agrijanti, Agrijanti Aqila Fadia Ari Khusuma Awan Dramawan Baiq Linda Rolian ocatari Erlin Yustin Tatontos Eustachius Hagni Wardoyo Fadhilah Rizkiyah Fakhmi, Shausan Nabila Fihiruddin, Fihiruddin Fitri Getas, I Wayan Gunarti Gunarti I Gusti Ayu Ratih Yuliartini Ayu I Made Suardika I Wayan Getas I Wayan Getas Intan Suryawatie Yomo, Meidiana Iswari Pauzi Iswari Pauzi Lalu Sri Gede Lalu Srigede Lalu Srigede Lalu Srigede Lalu Srigede, Lalu Lidia Febrianisa Maruni Wiwin Diarti Maruni Wiwin Diarti Meidiana Intan Suryawatie Yomo Muhammad Luthfi Nandini, Ni Kadek Ni Kadek Defyantari Ni Luh P. Sri Ayu Sutami D Ni Luh Putu Pebrita Nurul Inayati Nurul Inayati Nurul Inayati Nurul Inayati Pancawati Ariami Pancawati Ariami Permatasari, Lina Pramoswari, Dining RAI WIADNYA, IDA BAGUS Rohmi Rohmi, Rohmi Safitri, Wiwin Safitri, Wiwin Siti Karyani Siti Zaetun Siti Zaetun Siti Zaetun Siti zaetun Siti Zaetun, Siti Sri Hartuti Suaryana, I Made Chandra Thomas Tandi Manu Vani Nurcahyanti Viefa Avrilian Alifah Wadnya, Ida Bagus Rai Wayan Getas Wiwin Safitri Wulandari, Ayu Nurislami Yudha Anggit Jiwantoro Yunan Jiwintarum Zaenal Fikri Ziana Dia Ningsih