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Sosialisasi Deteksi Dini Penyakit Tidak Menular dan Pemeriksaan Kesehatan dengan Metode POCT Wirdullutfi; Baiq Isti HIjriani; Heru Setiawan; Eni Suyantri; Marosa Robi’atul Adawiyah
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.14141

Abstract

This community service program aims to enhance participants' knowledge, awareness, and independence in the early detection of non-communicable diseases (NCDs) through blood pressure, blood glucose, and uric acid screening, as an effort to control NCD cases in line with the 2030 Sustainable Development Goals (SDGs) for global well-being. The methods implemented included socialization and health screenings for 55 participants (average age 41 years; range 26-56 years; 67% male, 33% female), with 50 participants screened for blood glucose and uric acid (74% male, 26% female). Screening results showed: normal blood pressure in 45 people (81.9%), prehypertension in 8 people (14.5%), stage 1 hypertension in 2 people (3.6%); normal blood glucose in 44 people (88%), prediabetes in 5 people (10%), diabetes in 1 person (2%); and normal uric acid levels in 47 people (94%), hyperuricemia in 3 people (6%). All participants understood the importance of early NCD detection and committed to creating a healthier and more productive community.
Effect of Follicular Substances on Phytochemical Profile and Antioxidant Capacity of Ketip Banana (Musa paradisiaca Forma typiaca) Peel Ethanol Extract Bahri, Syamsul; Hijriani, Baiq Isti; Wirdullutfi, Wirdullutfi
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.10868

Abstract

A healthy lifestyles require both synthetic antioxidants and those sourced from food. Antioxidants can be obtained from food ingredients or can also be synthetic oxidants. Antioxidants derived from plants contain quite high levels. One type of tropical fruit contains antioxidants is the banana, and one cultivar of banana is Musa paradisiaca Forma typiaca, locally name is Ketip. The research aimed to compare the phytochemical profile of peel ethanol extract of this cultivar using different sample preparation techniques and then compare the antioxidant capacity of the extracts using the DPPH method. This research employed two types of techniques in preparing the sample. The first one leaves the sap attached to the sample, the second one uses tissue paper to reduce the follicular substances, soon after chopping the sample. Phytochemical results of the extract prepared by the first technique showed that the extract contains flavonoids, alkaloids, saponins, terpenoids, and tannins, while the extract prepared by the second technique also detected phenolic compounds. Despite the phytochemical profile showing no difference at all from both techniques, their antioxidant capacity showed a significant difference. The IC50 score of the extract prepared by the first technique was 554,84. This score is categorized in the very weak activity group, whereas the IC50 score of the extract prepared by the second technique was 202,13. This score is categorized in the intermediate activity group. It is concluded that the follicular substances significantly increase the IC50 score of ethanol extract of Ketip banana peel.
Phytochemical Screening of Methanol Extract of Neem (Azadirachta indica) Leaves Bahri, Syamsul; Hijriani, Baiq Isti; Widullutfi, Widullutfi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10998

Abstract

Neem (Azadirachta indica) is a highly respected medicinal plant and has been used in traditional medicine for centuries. The use of plants as medicine is important to know the secondary metabolite content in the plant. Phytochemical screening is a qualitative method aimed at tracing the presence of secondary metabolites contained in plants so that their potential as medicinal plants can be used. This research is an experimental laboratory using the maceration extraction method to obtain neem leaf extract. This research aims to identify secondary metabolite compounds contained in the ethanol extract of neem leaves. This study conducted flavonoid, tannin, alkaloid, steroid and saponin tests for the ethanol extract of neem leaves. The results obtained from the neem extract contain flavonoid, tannin, alkaloid, steroid and saponin compounds. In conclusion, phytochemical screening shows that the methanol extract of neem leaves (Azadirachta indica Juss) positively contains alkaloids, flavonoids, tannins, phenols, terpenoids, and steroids.
Antioxidant Activity of Methanol Extract Leaves of Neem (Azadirachta indica) Bahri, Syamsul; Wirdullutfi, Wirdullutfi; Hijriani, Baiq Isti
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.11048

Abstract

Azadirachta indica Juss. (neem), a medicinal plant belonging to the Meliaceae. The leaves of this species contain bioactive compounds such as flavonoids, alkaloids, steroids, saponins, tannins, and azadirachtin. These compounds exhibit antimicrobial activity against Salmonella and Escherichia coli. This study aimed to determine the antioxidant activity of the neem leaves' methanol extract by using the DPPH method. The research method used phytochemical screening by separating chemical compounds from samples using 70% methanol solvent. The result showed that the antioxidant activity of the neem leaves' methanol extract is classified in the weak category with an IC50 score of 426.15 mg/mL. One of the main factors explaining the high IC50 values is the differences in the chemical structure and molecular shape of the secondary metabolites contained in the extracts. The antioxidant activity of flavonoids and phenolics is greatly influenced by the number and position of hydroxyl groups, the degree of conjugation, and the presence of sugar groups.
Qualitative Phytochemical Screening of Ethanolic Extract of Ocimum basilicum L. Leaves Bahri, Syamsul; Wirdullutfi, Wirdullutfi; Hijriani, Baiq Isti
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.11142

Abstract

Ocimum basilicum L. (basil) leaves are widely recognized for their potential bioactive compounds. This study aimed to qualitatively evaluate the phytochemical constituents of ethanolic extracts of basil leaves. Dried basil leaves were extracted using 96% ethanol, and the resulting extract was subjected to standard phytochemical screening tests to identify major secondary metabolites. The results revealed a strong presence of alkaloids and steroids, indicating that these compounds are dominant constituents in the ethanolic extract. In addition, flavonoids, tannins, and phenolic compounds were detected at lower intensities, suggesting their presence in smaller amounts. Conversely, terpenoids and saponins were not detected in the extract. These findings indicate that ethanol is an effective solvent for extracting alkaloids and steroids from basil leaves, while its efficiency for terpenoids and saponins may be limited. The presence of these secondary metabolites supports the potential biological activity of Ocimum basilicum leaves and provides a scientific basis for further studies on their pharmacological and biological activities.  
Eksplorasi Kebutuhan Materi Ajar Mikrobiologi untuk Mempersiapkan Calon Guru yang Berdaya Saing dan Memiliki Keterampilan di Bidang Bioteknologi Sakaroni, Rubiyatna; Kusuma, Anindita Suliya Hangesti Mandra; Hijriani, Baiq Isti
Journal of Science and Education Research Vol. 5 No. 1 (2026): Journal of Science and Education Research
Publisher : Yayasan Pendidikan Insan Mulia Utan Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62759/jser.v5i1.397

Abstract

Pembelajaran Mikrobiologi pada program studi Pendidikan Biologi masih cenderung berorientasi pada penguasaan konsep teoretis dan belum sepenuhnya mendukung pengembangan keterampilan aplikatif mahasiswa yang relevan dengan perkembangan bioteknologi. Penelitian ini bertujuan untuk mengeksplorasi kebutuhan materi ajar Mikrobiologi mahasiswa Pendidikan Biologi Universitas Mataram dalam rangka mempersiapkan calon guru biologi yang berdaya saing dan memiliki keterampilan tambahan di bidang bioteknologi. Penelitian ini menggunakan pendekatan deskriptif kualitatif dengan teknik pengumpulan data berupa angket eksplorasi kebutuhan yang diberikan kepada 60 mahasiswa yang sedang atau telah menempuh mata kuliah Mikrobiologi. Data dianalisis melalui tahap reduksi data, penyajian data dalam bentuk tabel dan diagram, serta penarikan kesimpulan. Hasil penelitian menunjukkan bahwa mahasiswa memandang mata kuliah Mikrobiologi sebagai mata kuliah yang sangat penting, namun materi yang disajikan dinilai belum sepenuhnya kontekstual dan aplikatif. Mahasiswa menunjukkan kebutuhan yang tinggi terhadap bahan ajar berbentuk e-modul, berbasis riset, dan berorientasi pada proyek yang mengaitkan peran mikroorganisme dengan bioteknologi terapan. Selain itu, pemahaman mahasiswa terhadap peran mikroorganisme dalam bioteknologi masih bersifat parsial. Temuan ini menegaskan pentingnya pengembangan materi ajar Mikrobiologi yang kontekstual, berbasis riset, dan mendukung keterampilan aplikatif guna menunjang kompetensi profesional dan peluang pengembangan karier calon guru biologi.
Utilization of Filter Paper as an Innovative Transport Medium for Salmonella typhi and Shigella dysenteriae Bacteria: A Review of Effectiveness. Atfal, Bustanul; Hijriani, Baiq Isti; Maola, Seftia Dhina
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.112999

Abstract

The World Health Organization (WHO) released the Guidance on Regulations for the Transport of Infectious Substances 2023–2024 to minimize the risk of disease transmission caused by mishandling or leakage of infectious materials during transport. This initiative is reinforced by the Indonesian Directorate General of Civil Aviation, which restricts the amount of liquid material allowed on both domestic and international flights. In line with these policies, this study aimed to assess the effectiveness of filter paper as an alternative transport medium for Gram-negative bacteria, Salmonella typhi and Shigella dysenteriae, based on different storage durations at 37°C in the URB-Balitbangkes Laboratory in RSUP West Nusa Tenggara. The research employed an experimental laboratory design, using six samples with three repetitions, which resulted in 18 culture cups for each bacterium. Biochemical and Gram-staining tests confirmed positive results for both S. typhi and S. dysenteriae on the filter paper transport media. Bacterial growth remained consistent (+3) across all storage periods of 1, 2, 3, 4, 7, and 14 days. The findings demonstrate that filter paper can function effectively as a dry preservation medium, offering a practical and safe method for transporting infectious bacterial cultures over time.
Exploitation of Antibiofilm Substances by Endophytic Bacteria of Renggak Fruit (Amomum dealbatum) Against Staphylococcus aureus, the Cause of Nosocomial Infections Hijriani, Baiq Isti; Atfal, Bustanul; Kamila, Natasya Cahya
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.113070

Abstract

Nosocomial infections can be caused by biofilm-forming microorganisms. The ability to form biofilms results in persistent, recurrent, and 100-1000 times more resistant to antibiotics. One of the biofilm-forming bacteria is Staphylococcus aureus. The renggak plant (Amomum dealbatum Roxb.) which contains alkaloids, steroids, flavonoids, terpenoids, tannins, and saponins is known to play a role in inhibiting bacterial biofilm formation. This study used an in vitro experimental design aimed to analyzing the antibiofilm activity of endophytic bacteria against Staphylococcus aureus using the plate tissue culture method. The results obtained five endophytic bacterial isolates, including BI1, BI2, BI3, BI4, and BI5. The percentage of biofilm inhibitory activity by cell-free supernatants of endophytic bacteria was 35%, 0%, 37%, 44%, and 39%, respectively, with a positive control was 42%. Meanwhile, the biofilm revealing activity was 39%, 42%, 41%, 38%, and 36%, respectively, with a positive control was 56%. The results of the One Way ANOVA statistical test obtained a significance result of 0.000 (p<0.005), which means there was a significant difference between the treatment groups in the antibiofilm test. The conclusion is the cell-free supernatant of endophytic bacteria from renggak fruit has inhibitory and degradation activity against Staphylococcus aureus bacterial biofilms.
Penerapan Pembelajaran Berbasis Aktivitas Bermain untuk Meningkatkan Kemandirian Anak SLB Yelli Fitri; Rini Adriani Auliana; Lianda Dewi Sartika; Anak Agung Ngurah Bagus Janitra Dewanta; Ayunda Wulandari; Wirdullutfi; Baiq Isti Hijriani
Jurnal Pengabdian Magister Pendidikan IPA Vol 9 No 2 (2026): April-Juni 2026
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v9i2.15169

Abstract

This community service aimed to improve the independence of students in a Special School (SLB) through structured play-based learning. The main issue addressed was low student engagement and independence due to limited teaching methods. The activity was conducted through preparation, implementation, and evaluation stages by applying structured play activities such as guessing sounds and imitating animal movements. Evaluation was carried out through observation of participation, ability to follow instructions, and student independence. The results showed improvements in engagement, ability to follow instructions, and independence, indicated by reduced reliance on teacher assistance. The learning environment also became more enjoyable and interactive, encouraging students to be more active and confident. This activity provides a practical alternative for teachers to develop engaging and structured learning, and demonstrates that play-based learning can be an effective approach to enhance independence among students with special needs.
Co-Authors Adriyan Suhada Agung Dwi Wahyu Widodo Aini aini aini aini Aini, Aini Ainutajriani Ajeng Dian Pertiwi Ajeng Dian Pertiwi, Ajeng Dian Al-Fariz, Rifqi Aminullah, Andi Muhammad Anak Agung Ngurah Bagus Janitra Dewanta Anisa, Yufi Yuni Ayunda Wulandari Bustanul Atfal Denih Agus Setia Permana Detty Agustin Riscal Dewi, Fitriani Kusuma Dhika Juliana Sukmana Dwi Monika Ningrum Edy Kurniawan, Edy Eni Suyantri Fardani, Roushandy Asri Faujiah, Putri Hardani, H HERU SETIAWAN Irawansyah Irawansyah Ismatullah, Nurul Khatimah Kamila, Natasya Cahya Kusuma, Anindita Suliya Hangesti Mandra Liah Kodariah Lianda Dewi Sartika ManikRetno Wahyunitisari Maola, Seftia Dhina Marosa Robi’atul Adawiyah Mentari, Ika Nurfajri Nurul Hadiatun Nurul Khatimah Ismatullah Pauzan RAI WIADNYA, IDA BAGUS Rini Adriani Auliana Sakaroni, Rubiyatna Siska Zafrida Siti zaetun Siti Zaetun, Siti Solihin Solihin Sri Idawati Sri Sahbany Syamsul Bahri Ulya, Tuhfatul ustiawaty, jumari Ustyawati, Jumari Widullutfi, Widullutfi Widya sriwarsih Wirdullutfi, Wirdullutfi Wulan Ratia Ratulangi Yelli Fitri