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All Journal Jurnal Bios Logos JSME MIPA UNIMA Jurnal Produksi Tanaman Indonesian Journal of Biotechnology Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus SOSCIED Vivabio : Jurnal Pengabdian Multidisiplin Edumedia: Jurnal Keguruan dan Ilmu Pendidikan Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Journal of Comprehensive Science Makara Journal of Science Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam (JURRIMIPA) Indonesian Biodiversity Journal Rumphius Pattimura Biological Journal Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika Jurnal Pengabdian Masyarakat dan Riset Pendidikan Indonesian Biodiversity Journal Polygon: Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam Algoritma: Jurnal Matematika, Ilmu Pengetahuan Alam, Kebumian dan Angkasa Jurnal Cendekia Ilmiah Konstanta : Jurnal Matematika dan Ilmu Pengelatuan Alam Advances in Tropical Biodiversity and Environmental Sciences Metamorfosa: Journal of Biological Science Open Access DRIVERset
Helen Joan Lawalata
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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EFFECT OF GEL FORMULATION CONTAINING Tridax procumbens AND Aloe vera LEAF EXTRACT ON THE HEALING OF SECOND-DEGREE BURNS IN MICE (Mus musculus) Maturbongs, Marsela; Setyawati, Iriani; Manampiring, Nonny; Lawalata, Helen; Taulu, Marthy; Rompas, Christny
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/n2vkg912

Abstract

Tridax procumbens L. and Aloe vera L. are plants commonly used in the treatment of burns. This study was conducted to determine the effect of giving a combination gel of T. procumbens and A. vera leaf extract on second-degree burn healing in mice. This study used 24 male mice (Mus musculus) which were divided into four treatment groups, namely positive control (KP, Bioplacenton®); negative control (KN, gel base); leaf extract of T. procumbens 0.5% and A. vera 0.5% (P1); and leaf extract of T. procumbens 1% and A. vera 0.5% (P2). The process of inducing burns on the backs of mice requires the use of a 1x1 cm iron, which is subjected to heat in a fire for three minutes. The iron is placed on the mice's back for five seconds, which leads to the development of a second-degree burn. The gel was applied twice a day for 21 days. The highest level of burn wound healing was recorded with the Bioplacenton® treatment, which achieved a full recovery of 100% on day 15. Following this, the combined gel treatment of 1% T. procumbens leaf extract and 0.5% A. vera also reached 100% healing by day 18. While it may not be as potent as Bioplacenton®, the gel formulation containing 1% T. procumbens and 0.5% A. vera leaf extract presents a viable alternative for treating second-degree burns.
CHARACTERISTICS OF TURTLE NESTING HABITAT ON POPARENG BEACH, TATAPAAN DISTRICT, SOUTH MINAHASA REGENCY, BUNAKEN NATIONAL PARK AREA Mukuan, Randi; Lawalata, Helen; Tengker, Anita; Setyawati, Iriani; Mege, Revolson Alexius
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/mbaj0069

Abstract

Turtles They are among the world's protected animals and one of the rare, almost extinct species. The preservation of their habitat and knowledge of the traits of turtle-friendly habitats are two ways that the rescue and protection of these turtles must go on. For rescue operations, turtle sustainability, and sustainable coastal management, research is being conducted on the features of the turtle nesting habitats on Popareng Beach. This research aims to determine the characteristics of turtle nesting habitats and identify the parameters that hinder turtles from nesting at Popareng Beach, Tatapaan District, South Minahasa Regency. The method used in this research is the descriptive-exploratory method. With characteristic parameters consisting of beach width measurement, beach slope, sand temperature, sand humidity, sand texture, beach vegetation, and the presence of predators. From the research results, it shows that station 1 and station 2 have results that are suitable for turtle nesting habitats, with values obtained at station 1 being 88.33% and station 2 being 92%. Meanwhile, at station 3, with an evaluation score of 72%, it is considered fairly suitable and the station has tolerable limitations.
THE EFFECT OF THE USE OF CHICKEN MANURE ON RED BEANS (Phaseolus vulgaris L.) Goo, Alince; Satiman, Utari; Lawalata, Helen; Tumbel, Ferny Margo; Wantania, Johanna
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/dc0z3470

Abstract

Red beans are a horticultural crop belonging to the annual legume group that grows upright and has a fairly high nutritional content. To obtain maximum growth and yield from red beans, several factors must be considered, including balanced fertilizer application. The factor of providing organic fertilizer from chicken manure with the right dosage is the target of this study. This study aims to determine the effect of red bean growth on the dosage of chicken manure fertilizer. This study was conducted from December 2024 to February 2025 and was carried out on the plantation land of the Department of Science Education, Faculty of Mathematics, Natural Sciences and Earth Sciences, Manado State University. The method used is a quantitative method with a type of experiment in the form of a Randomized Block Design (RAK) with five treatment combinations with each repeated three times. A0 = 0 (control), A1 = 1.00 kg, A2 = 1.50 kg, A3 = 2.00 kg, A4 = 2.50 kg, so there are 15 experimental bed units, and the data obtained are then analyzed using analysis of variance (ANOVA). The results of this study indicate that good plant height growth, number of leaves and stem diameter are found in treatment A1 with a dose of 1.00 kg of chicken manure fertilizer and A2 with a dose of 1.50 kg of chicken manure fertilizer because there is the most maximum growth.
Identification of Endophytic Bacteria from Water Hyacinth (Eichhornia crassipes) in Lake Tondano as Potential Agents for Lead Bioremediation Margi Worang; Helen J. Lawalata; Anita Tengker; Verawati Roring; Dany Posumah; Mercy Rampengan
Advances in Tropical Biodiversity and Environmental Sciences Vol. 10 No. 1 (2026): February 2026
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2026.v10.i01.p04

Abstract

Water hyacinth (Eichhornia crassipes) is a herbaceous plant known to accumulate heavy metals from polluted waters. In Lake Tondano, this plant grows abundantly and helps reduce lead (Pb) levels through bioremediation. This study aimed to identify the genera of endophytic bacteria from water hyacinth (E. crassipes) that have potential as bioremediation agents for lead. This study employed an exploratory, descriptive, quantitative laboratory approach. Samples were collected from E. crassipes plants found in Lake Tondano at three different locations. The isolation method used was direct plating. The research stages included bacterial isolation, lead resistance testing at 50 ppm, lead reduction testing at 10, 20, and 30 ppm, lead concentration analysis, and bacterial identification using morphological and biochemical tests. Three isolates (EG.A1.5, EG.A2.4, and EG.D3.4) were found to be resistant and capable of reducing lead levels. The reduction percentages reached 35.9%, 72.2%, and 55.5%, respectively. Based on morphological and biochemical characteristics, the bacterial genera of three isolates were identified as Salmonella, Enterobacter, and Bacillus.
EFEKTIVITAS SEDIAAN GEL EKSTRAK DAUN PACAR AIR DAN LIDAH BUAYA TERHADAP PENYEMBUHAN LUKA SAYAT MENCIT Gabriela Tasya Paat; Iriani Setyawati; Meity Neltje Tanor; Helen Joan Lawalata; Anita Constanci Christine Tengker; Nonny Manampiring
Edumedia : Jurnal Keguruan dan Ilmu Pendidikan Vol 10 No 1 (2026): Edumedia: Jurnal Keguruan dan Ilmu Pendidikan
Publisher : Universitas Kapuas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51826/edumedia.v10i2.1906

Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas kombinasi ekstrak daun pacar air (Impatiens balsamina, L.) dan lidah buaya (Aloe vera) dalam proses penyembuhan luka sayatan. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Hewan uji yang digunakan adalah mencit (Mus musculus) jantan dewasa yang dibagi dalam 5 kelompok, yaitu kontrol positif (F0, bioplacenton); kontrol negatif (F1, tanpa perlakuan); perlakuan formula sediaan gel dari ekstrak daun pacar air 2,5% dan lidah buaya 2,5% (F2); pacar air 5% dan lidah buaya 5% (F3); pacar air 10% dan lidah buaya 10% (F4). Bagian paha belakang kanan mencit yang sebelumnya telah dicukur dan diolesi alkohol 70% dibuat luka sayat dengan silet sepanjang 10 mm dan kedalaman 2 mm. Pemberian sediaan gel dilakukan setiap hari pada jam 10.00 WITA selama 12 hari. Hasil penyembuhan luka sayat yang paling tinggi ditunjukkan oleh kelompok F4 dan F3, di mana pada hari ke-8 luka sudah menutup sempurna. Hasil penelitian menunjukkan bahwa pemberian sediaan gel ekstrak daun tumbuhan pacar air 5% dan lidah buaya 5% lebih efektif dalam penyembuhan luka sayat daripada Bioplacenton.
GROWTH PRODUCTIVITY OF PAKCOY (Brassica rapa L.) AND LETTUCE (Lactuca sativa L.) PLANTS IN AQUAPONIC SYSTEMS Emma Moko; Jenifer Nasilia Vernanda Kansil Tangkuliwutang; Helen Joan Lawalata; Livana Dethris Rawung; Johanna Zusye Wantania; Dino Rahardiyan
Agrisaintifika: Jurnal Ilmu-Ilmu Pertanian Vol. 10 No. 2 (2026): Agrisaintifika
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/ags.v10i2.7480

Abstract

Aquaponics is a cultivation method that integrates freshwater fish farming with hydroponics or soil-less plant cultivation, where wastewater from fish ponds is used as a source of nutrients for plants. This aquaponics system not only produces fish and fresh vegetables, but also reduces waste and water usage, making aquaponics an ideal solution for cultivation in small urban areas. This study aims to analyze and compare the growth productivity of pakcoy (Brassica rapa L.) and lettuce (Lactuca sativa L.) in an aquaponics system. This study was conducted at the Lotta Agricultural Center (SAL), Pineleng District, Minahasa Regency, North Sulawesi, from October to December 2024. The research method used a Completely Randomized Design (CRD) with two groups, namely pakcoy and lettuce plants, each with 57 replicates. The data collected included plant height, leaf width, number of leaves, biomass, and leaf chlorophyll content. The results showed that the aquaponics system had a significant effect on the growth of both types of plants. Pakcoy showed faster growth and higher productivity than lettuce, with significant differences in plant height (F = 223.160, Sig. = .000), leaf width (F =121.793, Sig. = .000), number of leaves (F = 734.323, Sig. = .000), and total biomass. Chlorophyll content in pakcoy was also higher than in lettuce, indicating greater photosynthetic capacity. The aquaponics system effectively supports the growth of pakcoy and lettuce, with pakcoy showing a better response. The aquaponics system can be an efficient sustainable agriculture solution in resource use and reducing environmental impact.
The Effect of Glycerol on the Production of Environmentally Friendly Bioplastics From Cassava Peel (Manihot Esculenta) and Corn Cobs (Zea Mays) Merliana Christmas Turangan; Helen Joan Lawalata; Utari Satiman; Rievo Handry Djarang
Journal of Comprehensive Science Vol. 5 No. 2 (2026): Journal of Comprehensive Science
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/jcs.v5i2.4009

Abstract

Conventional plastics made from synthetic polymers have a low biodegradation rate, which contributes greatly to environmental pollution. The use of agricultural waste such as cassava husks (Manihot esculenta) and corn cobs (Zea mays) as bioplastic raw materials is one of the alternatives for the development of environmentally friendly materials. This study aims to determine the effect of variations in the amount of glycerol and the length of decomposition time on the biodegradability of bioplastics made from cassava husks and corn cobs. The study used an experimental method with a Two-Way Factorial Design, covering three concentrations of glycerol (0 mL, 15 mL, and 30 mL) and three decomposition times (3, 6, and 9 days). The results showed that the treatment without glycerol (0 mL) did not form bioplastics so it could not be analyzed. The data was tested using Two Way ANOVA. The results of the biodegradability test showed that the glycerol concentration had a significant effect, and the 30 mL formulation resulted in the highest degradation rate of 91.026% on day 9.
Inhibitory Test of Water Hyacinth Leaf Extract (Eichhornia Crassipes) on the Growth of Escherichia Coli and Propionibacterium Acnes Bacteria Rumyaan, Yuliana; Helen J. Lawalata; Dany Ch. Posumah; Emma M. Moko; Fernando A. Watung; Nonny Manampiring
JURNAL RISET RUMPUN MATEMATIKA DAN ILMU PENGETAHUAN ALAM Vol. 5 No. 2 (2026): Agustus : JURRIMIPA: Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrimipa.v5i2.9447

Abstract

Antimicrobial resistance has accelerated the search for alternative antibacterial agents derived from natural products. Water hyacinth (Eichhornia crassipes) is an invasive aquatic weed that is abundant in Indonesian waters, yet rich in secondary metabolites such as flavonoids, tannins, alkaloids, and saponins with antibacterial potential. This study aimed to evaluate the inhibitory activity of the ethanol extract of water hyacinth leaves against Escherichia coli (Gram-negative) and Propionibacterium acnes (Gram-positive). A descriptive-exploratory laboratory design was used. Leaf samples collected from Lake Tondano were extracted by maceration in 96% ethanol and concentrated using a rotary evaporator. Antibacterial activity was assessed by the well-diffusion method at 100% extract concentration, with 30% amoxicillin as the comparator, incubated at 37°C for 24 hours. The extract produced mean inhibition zones of 7.0 mm against E. coli and 5.7 mm against P. acnes, both classified as moderate according to the Davis & Stout scheme, whereas amoxicillin produced zones of 8 mm and 7 mm. The ability of the extract to inhibit both Gram-positive and Gram-negative bacteria indicates a broad spectrum of activity. These findings confirm that water hyacinth leaves are a promising source of natural antibacterials while adding value to aquatic weed management.
Co-Authors Akay, Fanesa Andini Maurent Dwiyanti Anita Constanci Christine Tengker Anita Costanci Christine Tengker Anita Costanci Christine Tengker Aser Yalindua Bricita Regina Warangkiran Britney Meissy Pandoh Christny Ferdina Evie Rompas Danny Christian Posumah Danny Christian Posumah Dany Posumah Daud, Putri Debby Jacqualine Jochebed Rayer Decky D. W. Kamagi Decky David Wewurid Kamagi Delvy Regina Leunufna Desire Atna Sridanty Rumondor Dino Rahardiyan Dino Rahardiyan Djarang, Rievo H Elsa Aru Elsi Elsi Emma M Moko Endang Sutriswati Rahayu Enjel Larega Esibrena Br Kemit Esrah Anna Adeleid Agimat Etin Ruban Febiola Gabriela Tular Feby Febrianty Toding Rante Fernando Andre Watung Futwembun, Fransiska Gabriela Tasya Paat Gabrielle Warouw Genade Kojongian Goo, Alince Helena, Maria Helma Fitriani Herry Maurits Sumampouw Hotni Doani Purba IRIANI SETYAWATI Jacklin Stella Salome Manoppo Jantje Ngangi Jantje Ngangi Jefry Jack Mamangkey Jelita Pricilya Tompolumiu Jenifer Nasilia Vernanda Kansil Tangkuliwutang Johanna Zusye Wantania Kansile, Elma Fridayanti Kembuan, Patricia Pericilia Langkah Sembiring LANGKAH SEMBIRING Letsoin, Imanuel Junisa Adolfina Lidya Yangse Lumowa Livana Dethris Rawung Luis Sandi Najoan Maiko Polandos Maradesa, Sartika Marchio Yosua Gratia Mamesah Margi Worang Mariana Rengkuan Marsanda Tandiapa Marthy L S Taulu Marthy Lingkan Stela Tauluh Marthy Lingkan Stella Taulu Marthy Stella Taulu Maturbongs, Marsela Megawati Meylin Weken Meike Paat Meity Nelltje Tanor Meity Neltje Tanor Melany Piri Mercy Maggy Franky Rampengan Merliana Christmas Turangan Metilistina Sasinggala Michellia Pamela Sanggor Miracle Abbighael Varencia Welan Mokoginta, Ayatola R. Mokosuli Yermia Samuel Mokosuli, Yeremia Samuel Mukuan, Randi Mulder, Keysia Stephanie Tesalonica Muli, Efel J. R. Nangoy, Wiesye M. S> Nangoy, Wiesye Maya Selfia Natalina Zalukhu Nofia Poluan Nonny Manampiring Nova L.I.M Ogi Nuriyati Hadia Orbanus Naharia Patricia Mardiana Silangen Pelealu, Irna W. Posumah, Danny Posumah, Danny Christian Rahardiyan, Dino Rahayu, Endang Sutriswati Rampengan, Mercy Maggy Franky Regina R. Butarbutar Revanda Stevanie Hasani Revolson Mege Rey, Feyne Jelita Rievo Handry Djarang Rosenta Sipayung Rumyaan, Yuliana Runtunuwu, Yudistira Deyvan Runtuwene, Kharly Sakul, Ernest Hanny Sakul, Zefanya Escriva Rafael Salaila, Tania Jennet Sambeka, Delvino Sambuaga, Natalia V. Samuel, Mokosuli Y. Semuel Harpandi Simbolon, Kristina Br Sisilia Domitila Sangur Stanny Sicilia Rawung Sukmarayu Pieter Gedoan Tanorl, Meity N. Tatebale, Rivaldo Tedang, Bybyani Theresia Viviani Putri Ndese Tuelah, Syela Nathasya Utari Satiman Verawati I Y Roring Watugigir, Gerfindo Y. G Wiesye Maya Selfia Nangoy Wiesye Nangoy Yeremia S. Mokosuli YOSUA LINU Yulianus Rompah Matana Zusje Wiesje Merry Warouw