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All Journal Jurnal Bios Logos JSME MIPA UNIMA Jurnal Produksi Tanaman Indonesian Journal of Biotechnology Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus SOSCIED Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Makara Journal of Science Indonesian Biodiversity Journal Rumphius Pattimura Biological Journal Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika Jurnal Pengabdian Masyarakat dan Riset Pendidikan Indonesian Biodiversity Journal Polygon: Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam Algoritma: Jurnal Matematika, Ilmu Pengetahuan Alam, Kebumian dan Angkasa J-CEKI Konstanta : Jurnal Matematika dan Ilmu Pengelatuan Alam Advances in Tropical Biodiversity and Environmental Sciences
Helen Joan Lawalata
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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INVENTARISATION OF ORCHID TYPES (Orchidaceae) IN WEST TOMOHON AREA Miracle Abbighael Varencia Welan; Verawati Ida Yani Roring; Helen Joan Lawalata
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6585

Abstract

This study aims to inventory orchid species in the West Tomohon area using the accidental sampling method of exploration in a predetermined place. Orchids are a type of flowering plant that comes from the Orchidaceae family. Each type of orchid has its own characteristics. Tomohon City has abundant natural resources, including ornamental plants such as orchids. Data on orchid diversity in West Tomohon Sub-district has never been reported. Orchid inventory is an activity that records the diversity of orchid species in an area. This research is descriptive-qualitative research. The results of this study have yielded 10 species of orchids belonging to nine genera in one family. The orchid species found in the West Tomohon area consist of Arachnis sp., Arundina graminifolia, Cattleya sp., Dendrobium biggibum, Dendrobium moschatum, Epidendrum secundum, Oncidium flexuosum, Phaius tankervilleae, Phalaenopsis amabilis, and Vanda arcuata. The most common orchid found in this area is the moon orchid, Phalaenopsis amabilis.
Diversity of Echinoderms on the Beach of Ranowangko II Village, Kombi District, Minahasa Regency Letsoin, Imanuel Junisa Adolfina; Rampengan, Mercy Maggy Franky; Roring, Verawati Ida Yani; Lawalata, Helen Joan
RUMPHIUS Vol 6 No 1 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i1p48-55

Abstract

This research is motivated by the lack of studies and absence of research data on the diversity of Echinoderms on the Beach of Ranowangko II Village, Kombi District, Minahasa Regency. The aim of this study is to determine the diversity of the phylum Echinodermata found on the Beach of Ranowangko II Village, Kombi District, Minahasa Regency. Sampling in this research used the quadrat transect method with 3 transect lines, each line being 100 m long, and 10 quadrats, each measuring 1 x 1 m². The results of the study found 4 classes and 5 species including the class Asteroidea (Protoreaster nodosus), class Ophiuroidea (Ophiocoma scolopendrina), class Echinoidea (Tripneustes gratilla), class Holothuroidea (Holothuria atra & Holothuria vagabunda), and there were 21 individuals, and the dominant species is Holothuria atra from the Holothuroidea class of which there are 11 individuals, indicating that the diversity index (H’) of the phylum Echinodermata on the location is moderate with a value of 1.204. These findings offer initial insights into the condition of the local ecosystem, classified as moderate, and may serve as a foundational reference for long-term ecological monitoring.
KARAKTER KIMIA DAN PASTING PROPERTIES PATI JAGUNG (Zea mays) HASIL EKSTRAKSI MENGGUNAKAN NATRIUM METABISULFIT Moko, Emma Mauren; Rey, Feyne Jelita; Lawalata, Helen Joan; Rawung, Livana Dethris; Rahardiyan, Dino
Jurnal Sains dan Teknologi Pangan Vol 9, No 3 (2024):
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v9i3.48082

Abstract

Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh ekstraksi dengan perlakuan perendaman menggunakan natrium metabisulfit terhadap karakter pati jagung (Zea mays). Parameter yang diamati meliputi :kadar proksimat yaitu kadar abu, kadar protein, kadar karbohidrat, kadar lemak, dan kadar air), uji warna yaitu nilai L*, a* dan b*, karakter pasting properties yaitu peak viscosity, through viscosity, breakdoen viscosity, final viscosity, setback viscosity, peak time dan pasting temperature. Hasil penelitian menunjukan bahwa natrium metabisulfit memberikan pengaruh yang berbeda nyata terhadap kadar abu, protein, karbohidrat, lemak, air, warna, rendemen dan pasting properties.
Penerapan Model Pembelajaran TPS Untuk Meningkatkan Hasil Belajar Siswa Pada Materi Virus Luis Sandi Najoan; Helen J. Lawalata; Mariana Rengkuan
Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika Vol. 2 No. 1 (2024): Februari : Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/arjuna.v2i1.395

Abstract

The Think Pair Share cooperative learning technique is highly effective in fostering diverse discussion patterns. The purpose of Think Pair Share learning is to shape student interaction habits. The Think Pair Share instructional approach provides students with extended opportunities for reflection, articulation, and collaborative assistance. The objective of this study is to enhance the academic performance of students in class X viral material at SMA Negeri 2 Tondano by applying the Think Pair Share instructional approach. The research methodology employed is known as Classroom Action Research (PTK), which is conducted in two cycles, with each cycle comprising four distinct stages: preparation, implementation/action, observation, and reflection. The research findings indicate that the implementation of the Think Pair Share (TPS) instructional approach has resulted in a significant enhancement of student learning outcomes in class 86.66% during cycle II. This indicates that the research findings have met the success criterion, specifically, that at least 75% of students have successfully finished.
Isolation, Identification, and Antibacterial Assay of Indigenous Bacterial Isolates from Apis cerana Honeycomb Simbolon, Kristina Br; Mokosuli, Yermia; Lawalata, Helen J
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3441

Abstract

Apis cerana is a honey bee species with a cavity hive known as the eastern honey bee. Honey bee hives have Indigenous bacteria that have antimicrobial potential. Indigenous bacteria are free bacteria that can synthesize nitrogen compounds, sugars, and other bioactive substances. This study aims to isolate, identify and test the antibacterial of Indigenous bacteria isolate Apis cerana against gram-positive Staphylococus aureus and gram-negative bacteria Escherichia coli. This study used a descriptive research method to obtain the data from laboratory experiments. The study consisted of isolating Indigenous bacteria, identifying and testing the antibacterial agar diffusion method. The study results obtained seven isolates of AC 1, AC 2, AC 3, AC 4, AC 5, AC 6, AC 7 Indigenous bacteria from Apis cerana nests. AC 1 isolate has similarities with the genus Streptococcus, AC 2 isolates have similarities with the Klebsiella genus, while AC 3 isolates have similarities with the Bacillus genus, and AC 4, AC 5, AC 6 and AC 7 isolates have similarities with the Citrobacter genus. Indigenous bacterial isolates with potential antibacterial potential where the most significant inhibition against Staphylococus aureus bacteria was shown by isolate AC 1 (10.79 mm). At the same time, the smallest was found in isolate AC 7 (8.52 mm). The most significant inhibition against Escherichia coli bacteria was shown by isolate AC 1 (9.0 mm) while the smallest was found in isolate AC 3 (7.4 mm). Apis cerana nests have indigenous bacteria that have the potential to produce antibacterial substances
Antimicrobial Activity of Endophyte Bacteria Isolated from Seaweed (Padina sp.) on the Growth of Bacteria Escherichia coli and Staphylococcus aureus Akay, Fanesa; Ngangi, Jantje; Lawalata, Helen J
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.3632

Abstract

Padina sp. seaweed is one of the Chlorophyta which has potential as an antimicrobial. This study aimed to determine the antimicrobial activity of Padina sp. seaweed endophytic bacteria against Escherichia coli and Staphylococcus aureus. The method used in this research is descriptive qualitative. The results of the isolation of endophytic microbes of Padina sp. seaweed obtained were seven isolates DP1, DP2, DP3, DP4, AP1, AP2, and AP3. The results of the antimicrobial activity test showed that the seven isolates of endophytic bacteria had potential as antimicrobials. Incubation time for 24 hours showed that the diameter of the inhibition zone formed was 9.75 – 11.55 mm against S. aureus and 7.15 – 10.0 mm against bacteria. E. coli. The AP3 and DP1 isolates had a wider zone, presumably because their antibacterial substances were higher than the other isolates.
Effectiveness of Chicken Manure Flower Extract (Tagetes ercta L.) Against Mortality and Antifeedant in Chili Aphid Pests Myzus persicae sulz Hotni Doani Purba; Helen J. Lawalata; Utari Satiman; Aser Yalindua; Christny F. E. Rompas
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6291

Abstract

This research was motivated by the chicken dung flower plant (Tagetes erecta L.) which is one of the plants that is often found in several areas in North Sulawesi, containing active chemical compounds such as alkaloids, terpenoids, saponins, tannins and flavonoids which can improve health. improve health. improve health. improve health. improve health. improve health. used as a vegetable protector. This study aims to determine the effectiveness of chicken manure flower extract on the mortality and antifeedant effect of chili aphids Myzus persicae sulz. This examination utilized an exploratory technique utilizing the leaf separate strategy utilizing a Totally Randomized Plan (CRD) design comprising of 4 treatments and 4 replications. Each treatment was given chicken manure flower extract with respective concentrations, namely P0 (0%), P1 (5%), P2 (10%), and P3 (15%). The results showed that the administration of chicken manure flower extract had a significant effect on the death of the chili aphid Myzus persicae sulz. The higher the extract from chicken manure flowers the higher the death rate of chili aphids Myzus persicae sulz and chicken manure flowers have an antifeedant effect at a concentration of 15%. So it can be concluded that giving chicken manure flower extract is effective in increasing the mortality of Myzus persicae sulz at a concentration of 15%, namely an average of 4.75 birds and has an antifeedant effect at a concentration of 15.%, namely an average of 0.350 grams.
SUKSESI BAKTERI PADA FERMENTASI KULIT BUAH NANAS (Ananas comosus (L.) Merr) Pelealu, Irna W.; Lawalata, Helen J.; Tengker, Anita C.; Posumah, Danny
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.861

Abstract

The utilization of pineapple peel in the fermentation process produces bacteria and probiotics that are beneficial for human health. The fermentation process is carried out to enhance the nutrition, shelf life, and organoleptic properties of food products. The presence of microbes, especially bacteria, is believed to play an important role in the fermentation process of pineapple peel. However, the specific bacteria involved in the fermentation of pineapple peel are not yet fully known. The aim of this research is to study the bacterial succession during the fermentation of pineapple peel (Ananas comosus (L.) Merr) and to identify the types of bacteria obtained during the fermentation process. This research uses a qualitative descriptive laboratory-based method by conducting morphological identification of bacteria and biochemical testing to determine the types of bacteria present in the fermentation of pineapple peel. Based on the identification data, 11 bacterial isolates were found during the fermentation of pineapple peel. The isolates were identified macroscopically and then subjected to a series of biochemical tests. The results of the research indicate that the microorganisms involved in the fermentation of pineapple peel belong to the genera Staphylococcus sp., Bacillus sp., and Lactobacillus sp.. The microbial succession during pineapple peel fermentation shows that the bacterial population of the genus Staphylococcus sp. dominates on day 0 of fermentation, while the genus Bacillus sp. undergoes a succession pattern, experiencing growth on day 1 and continuing to increase until day 3. Meanwhile, bacteria from the genus Lactobacillus sp. only appeared on day 1 of fermentation.
Isolasi dan Identifikasi Bakteri Penyebab Penyakit pada Ikan Nila (Oreochromis niloticus) di Desa Tatelu Kecamatan Dimembe Kabupaten Minahasa Utara Watugigir, Gerfindo Y. G; Rayer, Debby J. J.; Lawalata, Helen J; Watung, Fernando A; Rampengan, Mercy M. F
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.870

Abstract

Tilapia is a freshwater animal that is always in contact with the aquatic environment so that it is easily infected by bacteria through water. This study aims to identify the types of bacteria that cause diseases that attack Tilapia (Oreochromis niloticus) kept in Dimembe District, North Minahasa Regency. This study uses a descriptive exploratory research method. The results of the study found sixteen isolates and identified them as bacteria that cause Tilapia disease in North Minahasa Regency, including Aeromonas sp. (4 isolates), Plesiomonas sp. (5 isolates), and Enterobacter sp. (1 isolate). based on morphological characteristics (colonies and bacterial cells) and biochemical (catalase, oxidase and motility tests). It was found that periodic and continuous fish disease surveillance is needed to monitor the spread of fish diseases, especially Tilapia disease in North Minahasa
IDENTIFIKASI BAKTERI YANG TUMBUH PADA OLAHAN ABON IKAN CAKALANG (Katsuwonus pelamis L.) DARI UMKM KOTA BITUNG Daud, Putri; Naharia, Orbanus; Lawalata, Helen Joan; Setyawati, Iriani; Nangoy, Wiesye M. S>
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.875

Abstract

Skipjack Tuna (Katsuwonus pelamis L.) is a pelagic fish that dominates the waters of the Indian and Pacific Oceans. This fish has unique physical characteristics, including an aerodynamic body, a metallic blue or teal back, and transverse stripes that distinguish it from other species. In Indonesia, skipjack tuna is abundant in the waters of Manado and Maluku. This study aims to determine the types of bacteria present in processed skipjack tuna floss (abon cakalang) based on morphological and physiological characteristics through biochemical tests. This study employs a qualitative descriptive approach conducted in a laboratory setting. Data were gathered through direct observation of samples undergoing various tests and subsequently presented in a descriptive manner. Based on the identification data, 30 bacterial isolates were found in the processed skipjack tuna floss. The isolates were identified macroscopically and microscopically. The findings of the study reveal that the bacteria found in processed skipjack tuna floss, based on biochemical tests and profile matching, are suspected to belong to the Staphylococcus and Vibrio genera.
Co-Authors Akay, Fanesa Andini Maurent Dwiyanti Anita Constanci Christine Tengker Anita Costanci Christine Tengker Anita Costanci Christine Tengker Aser Yalindua Bricita Regina Warangkiran Christny Ferdina Evie Rompas Danny Christian Posumah Daud, Putri Debby Jacqualine Jochebed Rayer Decky D. W. Kamagi Decky David Wewurid Kamagi Delvy Regina Leunufna Dino Rahardiyan Elsa Aru Emma M Moko Endang Sutriswati Rahayu Enjel Larega Esibrena Br Kemit Esrah Anna Adeleid Agimat Etin Ruban Febiola Gabriela Tular Feby Febrianty Toding Rante Fernando Andre Watung Futwembun, Fransiska Gabrielle Warouw Genade Kojongian Helena, Maria Helma Fitriani Herry Maurits Sumampouw Hotni Doani Purba IRIANI SETYAWATI Iriani Setyawati Iriani Setyawati Jantje Ngangi Jefry Jack Mamangkey Jelita Pricilya Tompolumiu Kansile, Elma Fridayanti Kembuan, Patricia Pericilia Langkah Sembiring LANGKAH SEMBIRING Letsoin, Imanuel Junisa Adolfina Lidya Yangse Lumowa Livana Dethris Rawung Luis Sandi Najoan Maiko Polandos Manampiring, Nonny Maradesa, Sartika Marchio Yosua Gratia Mamesah Mariana Rengkuan Marsanda Tandiapa Marthy L S Taulu Marthy Lingkan Stela Tauluh Marthy Lingkan Stella Taulu Marthy Stella Taulu Megawati Meylin Weken Meity Nelltje Tanor Meity Neltje Tanor Melany Piri Mercy Maggy Franky Rampengan Metilistina Sasinggala Meyke Paat Michellia Pamela Sanggor Miracle Abbighael Varencia Welan Moko, Emma Mokoginta, Ayatola R. Mokosuli Yermia Samuel Mokosuli, Yeremia Samuel Mulder, Keysia Stephanie Tesalonica Muli, Efel J. R. Nangoy, Wiesye M. S> Natalina Zalukhu Nofia Poluan Nonny Manampiring Nova L.I.M Ogi Nuriyati Hadia Orbanus Naharia Orbanus Naharia Pelealu, Irna W. Posumah, Danny Posumah, Danny Christian Rahardiyan, Dino Rahayu, Endang Sutriswati Rampengan, Mercy Maggy Franky Regina R. Butarbutar Revanda Stevanie Hasani Revolson Mege Rey, Feyne Jelita Rievo Djarang Rievo Handry Djarang Rompas, Christny Ferdina Evie Roring, Verawati I. Y Roring, Verawati I. Y. Rosenta Sipayung Runtunuwu, Yudistira Deyvan Salaila, Tania Jennet Sambeka, Delvino Sambuaga, Natalia V. Samuel, Mokosuli Y. Simbolon, Kristina Br Sisilia Domitila Sangur Tanorl, Meity N. Tatebale, Rivaldo Tedang, Bybyani Theresia Viviani Putri Ndese Tuelah, Syela Nathasya Utari Satiman Verawati I Y Roring Verawati I.Y. Roring Verawati I.Y. Roring Verawati Ida Yani Roring Watugigir, Gerfindo Y. G Wiesye Maya Selfia Nangoy Wiesye Nangoy Yeremia S. Mokosuli Yermia Samuel Mokosuli YOSUA LINU Yulianus Rompah Matana Zusje Wiesje Merry Warouw