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SUCCESSION OF LACTIC ACID BACTERIA ON CANDIED PAKOBA FRUIT (Syzygium luzonense) Kembuan, Patricia Pericilia; Mokosuli, Yeremia Samuel; Lawalata, Helen J.; Moko, Emma; Roring, Verawati I. Y
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.878

Pakoba fruit is often made into sweets, salad, pickles because it has a very sour taste and is a habitat for lactic acid bacteria. The purpose of this study was to determine the characteristics of lactic acid bacteria succession during the fermentation process of candied pakoba fruit. This research is an explorative research. The number of microbes was observed starting from day 0, 1, 3 and 7. Based on the results of identification data, 12 bacterial isolates were obtained in pakoba fruit fermentation. The isolates obtained were then macroscopically identified and microscopically identified. 12 isolates were round, 12 isolates had enpitire edges, 12 isolates had raised elevations, 12 isolates had covex colony surfaces, 10 isolates were white and 2 isolates were cream-colored. Of the 12 isolates, it was found that the bacteria that grew in the fermentation of candied pakoba fruit after profile matching was suspected of the genus Lactobacilus sp. and Leucunostoc.

ANALISIS STRUKTUR DAN POLA PERTUMBUHAN IKAN PAYANGKA (Ophieleotris aporos) DI DANAU LIMBOTO Mokoginta, Ayatola R.; Mamangkey, Jefry J.; Rampengan, Mercy M. F; Ogi, Nova L I M; Lawalata, Helen J.
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.888

Payangka fish (Ophieleotris aporos) is a fish that lives in freshwater and has the ability to survive in the midst of competition with other fishes that are very tough. This study aims to analyze the structure of payangka fish and the growth pattern of payangka fish in Lake Limboto. Fish samples were caught using a 2 cm net with a net length of 7 m and then a 1 m serok (sibusibu) with a total sample of 1003 fish. The results of the length structure analysis of payangka fish obtained a length range of 9.15 - 19.05cm with an average of 12.45 cm and the results of the weight structure analysis obtained a range of 9.27 - 47.97 grams with an average of 13.57 grams. The results of the analysis of the relationship between length and weight of payangka fish obtained allometric growth pattern (b = 2.92), b < 3 which means that weight gain is faster than length gain..

Endophytics Bacteria from Pangi Fruit (Pangium edule REINW.) and Its Potential as Producer of Antibacterial Compound Sambuaga, Natalia V.; Samuel, Mokosuli Y.; Lawalata, Helen J.
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 7 No. 2 (2024): Oktober 2024
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55724/jbiofartrop.v7i2.470

Natural antibacterials can be derived from various types of plants, one of which is the Pangi plant (Pangium edule REINW.). Pangi fruit contains an antioxidant and antibacterial compound that can inhibit bacterial growth. This study aims to determine the isolates of endophytic bacteria contained in Pangi fruit and its potential as a producer of antibacterial compounds against Escherichia coli and Staphylococcus aureus bacteria. This study used exploratory description research method. Results Isolation research of endophytic bacteria from Pangi fruit obtained seven isolates. Each Isolate has a different morphology both from the shape, color, edges, and colony elevation. Based on the results of antibacterial activity testing, each isolate of endophytic bacteria showed different clear zone diameters. Endophytic bacteria showed different clear zone diameters against E. coli (0.75 mm – 4.5 mm) and S. aureus (1 mm – 5 mm). Endophytic bacterial isolates That have the potential as antibacterial with the largest clear zone diameter are Isolate PA 2.1 (4.5 mm) against E. c oli and isolate PA 3.2 (5 mm) against the bacteria S. aureus. Based on the results of morphological characterization of isolate PA 1.1 has similarities with the genus Basillus sp., isolates PA 1.2, PA 1.3 PA 2.2, PA 3.1 and PA 3.2 have similarity with the genus Basillus sp. PA 3.2 has similarities with the genus Paracoccus alcaliphilus and isolate PA 2.1 has similarities with the genus Alcaligenes sp.

APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.

ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category

TESTING FOR BACTERIAL CONTAMINATION ON CHICKEN (Gallus gallus domesticus) IN THE TRADITIONAL MARKET OF SOUTH LANGOWAN DISTRICT Sambeka, Delvino; Mege, Revolson; Setyawati, Iriani; Lawalata, Helen J; Rompas, Christny Ferdina Evie
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10754

Chicken meat (Gallus gallus domesticus) plays an important role in society with its role as one of the sources of fulfillment of animal protein in Indonesia. With the high consumption of chicken meat by the public, the hygiene and safety of chicken meat must be ensured so as not to incur losses to the people. This study aimed to determine the bacterial contamination based on the testing of Total Plate Counts, Coliform, Escherichia coli, and Salmonella sp. Meet the requirement of the Indonesian National Standard on chicken meat in the traditional market of Langowan Selatan District. The method used in this research is a descriptive method to analyze and describe the results of the study, the testing using several indicators to directly conclude bacterial contamination. The results of the total plate count bacterial contamination test of the colonies, TPC in sample 1 = 29,1x kol/, sample 2 = 29,4x kol/, sample 3 = 27,7x kol/. MPN of Coliform and Escherichia coli results obtained in samples S1, S2, and S3 are 11x kol/gr. The results of microbact Salmonella sp. In samples S1 are positive, and S2 and S3 are negative. Based on observations, it is suspected that contamination occurs because sellers use equipment that is less sterile and there is no cover or barrier so the chicken meat is in direct contact with the air and customer. It is concluded that based on bacterial testing indicators, chicken meat in the traditional market of Langowan Selatan District has exceeded the limit of SNI 7388 2009 and is contaminated.

IDENTIFICATION OF ESCHERICHIA COLI AND SALMONELLA SP. ON FRIED SNACKS SOLD ON THE UNIMA CAMPUS ENVIRONMENT Runtunuwu, Yudistira Deyvan; Lawalata, Helen J; Tengker, Anita
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10756

This study aims to explain how bacterial contamination and the presence of Escherichia coli and Salmonella sp. on fried bananas sold in the UNIMA campus environment. This exploration used clear qualitative subjective observation with the TPC strategy for all total bacterial contamination and involved specific media for Escherichia coli and Salmonella sp. From the results of the review, it was found that 1 out of 3 experimental examples of complete bacterial contamination exceeded the threshold set by the Food and Drug Supervisory Agency of the Republic of Indonesia (BPOM RI) in 2016, with the most extreme obstacle being the number of microorganisms in food being 10-4 colonies/ g. Of the 3 samples tried, all samples were identified with Escherichia coli and Salmonella sp. Based on the research conducted, it can be concluded that, of all the observed fried samples, all detected bacterial colonies

APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.

ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category

TESTING FOR BACTERIAL CONTAMINATION ON CHICKEN (Gallus gallus domesticus) IN THE TRADITIONAL MARKET OF SOUTH LANGOWAN DISTRICT Sambeka, Delvino; Mege, Revolson; Setyawati, Iriani; Lawalata, Helen J; Rompas, Christny Ferdina Evie
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10754

Chicken meat (Gallus gallus domesticus) plays an important role in society with its role as one of the sources of fulfillment of animal protein in Indonesia. With the high consumption of chicken meat by the public, the hygiene and safety of chicken meat must be ensured so as not to incur losses to the people. This study aimed to determine the bacterial contamination based on the testing of Total Plate Counts, Coliform, Escherichia coli, and Salmonella sp. Meet the requirement of the Indonesian National Standard on chicken meat in the traditional market of Langowan Selatan District. The method used in this research is a descriptive method to analyze and describe the results of the study, the testing using several indicators to directly conclude bacterial contamination. The results of the total plate count bacterial contamination test of the colonies, TPC in sample 1 = 29,1x kol/, sample 2 = 29,4x kol/, sample 3 = 27,7x kol/. MPN of Coliform and Escherichia coli results obtained in samples S1, S2, and S3 are 11x kol/gr. The results of microbact Salmonella sp. In samples S1 are positive, and S2 and S3 are negative. Based on observations, it is suspected that contamination occurs because sellers use equipment that is less sterile and there is no cover or barrier so the chicken meat is in direct contact with the air and customer. It is concluded that based on bacterial testing indicators, chicken meat in the traditional market of Langowan Selatan District has exceeded the limit of SNI 7388 2009 and is contaminated.

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